Enterococcus phoeniculicola ATCC BAA-412

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae; Enterococcus; Enterococcus phoeniculicola

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3510 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R3TGN0|R3TGN0_9ENTE Fructokinase OS=Enterococcus phoeniculicola ATCC BAA-412 OX=1158610 GN=UC3_03552 PE=4 SV=1
MM1 pKa = 7.46KK2 pKa = 10.0KK3 pKa = 9.58ILVISVVSGTMLFGMLLASNEE24 pKa = 3.9SHH26 pKa = 6.38AAEE29 pKa = 4.29WTANTPDD36 pKa = 5.03RR37 pKa = 11.84IQISDD42 pKa = 3.68GQTSYY47 pKa = 11.4TMVFGDD53 pKa = 4.1TLWAISQKK61 pKa = 9.89TNLNLQTLADD71 pKa = 4.18LNKK74 pKa = 9.75VNLLAGDD81 pKa = 3.62QYY83 pKa = 11.2GLQVGRR89 pKa = 11.84VISFDD94 pKa = 3.27GATVTVTEE102 pKa = 4.12SDD104 pKa = 3.57GTVVNQATVTDD115 pKa = 4.07EE116 pKa = 4.15NKK118 pKa = 10.05VNSEE122 pKa = 4.07QAVGQAVDD130 pKa = 3.84PTATEE135 pKa = 3.81QNYY138 pKa = 10.44GGSGLAASGYY148 pKa = 9.93VDD150 pKa = 4.3NGDD153 pKa = 3.49GTSTNTSTGEE163 pKa = 4.18VIDD166 pKa = 4.24NATGAPVTPTPTPTEE181 pKa = 4.1PSTPAEE187 pKa = 4.04PTEE190 pKa = 4.1PTEE193 pKa = 4.16PTEE196 pKa = 4.24PTDD199 pKa = 3.64PSEE202 pKa = 4.63PEE204 pKa = 4.24VPEE207 pKa = 4.27GPYY210 pKa = 10.74AGDD213 pKa = 3.15ILSGVEE219 pKa = 3.66NGLVGTYY226 pKa = 10.05TDD228 pKa = 5.7DD229 pKa = 3.68EE230 pKa = 5.25ALMDD234 pKa = 5.3AMDD237 pKa = 4.9AYY239 pKa = 10.08KK240 pKa = 10.53LANDD244 pKa = 3.88LHH246 pKa = 7.67GSEE249 pKa = 4.25WDD251 pKa = 3.67YY252 pKa = 11.74LILSNGYY259 pKa = 8.0GWYY262 pKa = 10.3AVWVNQQ268 pKa = 4.52

Molecular weight: 28.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R3U5S0|R3U5S0_9ENTE Protein RecA OS=Enterococcus phoeniculicola ATCC BAA-412 OX=1158610 GN=recA PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.34QPNKK9 pKa = 9.16RR10 pKa = 11.84KK11 pKa = 9.12RR12 pKa = 11.84QKK14 pKa = 9.38VHH16 pKa = 5.8GFRR19 pKa = 11.84KK20 pKa = 10.04RR21 pKa = 11.84MSTKK25 pKa = 9.46NGRR28 pKa = 11.84RR29 pKa = 11.84VLASRR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.13GRR39 pKa = 11.84KK40 pKa = 8.79VLSAA44 pKa = 4.05

Molecular weight: 5.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3510

0

3510

1105453

27

3052

314.9

35.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.657 ± 0.053

0.642 ± 0.011

5.017 ± 0.039

7.553 ± 0.051

4.81 ± 0.038

6.521 ± 0.048

1.765 ± 0.02

7.735 ± 0.04

7.073 ± 0.034

9.958 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.673 ± 0.022

4.631 ± 0.032

3.366 ± 0.021

4.086 ± 0.034

3.532 ± 0.031

6.364 ± 0.033

6.006 ± 0.044

6.765 ± 0.034

1.012 ± 0.016

3.835 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski