Staphylococcus phage YMC/09/04/R1988
Average proteome isoelectric point is 6.33
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 62 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|U5U4T5|U5U4T5_9CAUD Uncharacterized protein OS=Staphylococcus phage YMC/09/04/R1988 OX=1399949 GN=BPMRSA60_00580 PE=4 SV=1
MM1 pKa = 7.44 NNRR4 pKa = 11.84 EE5 pKa = 4.36 KK6 pKa = 10.46 IEE8 pKa = 3.81 QSVISASAYY17 pKa = 10.04 NGNDD21 pKa = 3.29 TEE23 pKa = 4.44 GLLKK27 pKa = 10.44 EE28 pKa = 4.35 VEE30 pKa = 4.22 DD31 pKa = 4.44 VYY33 pKa = 11.66 KK34 pKa = 10.66 KK35 pKa = 10.77 AQAFDD40 pKa = 4.66 EE41 pKa = 4.34 ILEE44 pKa = 4.2 GLPNAMQDD52 pKa = 3.39 ALKK55 pKa = 10.69 EE56 pKa = 4.39 DD57 pKa = 3.81 IEE59 pKa = 4.66 LDD61 pKa = 3.61 EE62 pKa = 5.16 AVGIMTGQVVYY73 pKa = 10.18 KK74 pKa = 10.68 YY75 pKa = 11.02 EE76 pKa = 3.83 EE77 pKa = 4.11 AQEE80 pKa = 3.91 NDD82 pKa = 3.02
Molecular weight: 9.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.266
IPC2_protein 4.05
IPC_protein 3.948
Toseland 3.77
ProMoST 3.999
Dawson 3.897
Bjellqvist 4.101
Wikipedia 3.783
Rodwell 3.783
Grimsley 3.681
Solomon 3.884
Lehninger 3.834
Nozaki 4.012
DTASelect 4.139
Thurlkill 3.795
EMBOSS 3.795
Sillero 4.05
Patrickios 3.567
IPC_peptide 3.884
IPC2_peptide 4.037
IPC2.peptide.svr19 3.993
Protein with the highest isoelectric point:
>tr|U5U457|U5U457_9CAUD Uncharacterized protein OS=Staphylococcus phage YMC/09/04/R1988 OX=1399949 GN=BPMRSA60_00170 PE=4 SV=1
MM1 pKa = 7.64 ARR3 pKa = 11.84 RR4 pKa = 11.84 KK5 pKa = 9.81 VIRR8 pKa = 11.84 VRR10 pKa = 11.84 IKK12 pKa = 10.5 GKK14 pKa = 10.6 LMTLRR19 pKa = 11.84 EE20 pKa = 4.12 VSEE23 pKa = 4.8 KK24 pKa = 10.69 YY25 pKa = 10.32 HH26 pKa = 6.64 ISPEE30 pKa = 4.02 LLRR33 pKa = 11.84 YY34 pKa = 9.46 RR35 pKa = 11.84 YY36 pKa = 8.24 KK37 pKa = 10.75 HH38 pKa = 6.21 KK39 pKa = 10.45 MRR41 pKa = 11.84 GDD43 pKa = 3.41 EE44 pKa = 4.19 LLCGRR49 pKa = 11.84 KK50 pKa = 9.44 DD51 pKa = 3.52 SKK53 pKa = 11.56 SKK55 pKa = 11.53 DD56 pKa = 3.15 EE57 pKa = 4.06 VEE59 pKa = 4.23 YY60 pKa = 10.11 MKK62 pKa = 10.94 SQIKK66 pKa = 10.52 DD67 pKa = 3.37 EE68 pKa = 4.26 EE69 pKa = 4.34 KK70 pKa = 10.23 EE71 pKa = 3.86 RR72 pKa = 11.84 EE73 pKa = 4.18 KK74 pKa = 10.64 IRR76 pKa = 11.84 KK77 pKa = 8.59 KK78 pKa = 10.87 AILNLYY84 pKa = 8.27 QRR86 pKa = 11.84 NVRR89 pKa = 11.84 AEE91 pKa = 3.91 YY92 pKa = 10.0 EE93 pKa = 4.17 EE94 pKa = 3.95 EE95 pKa = 3.87 RR96 pKa = 11.84 KK97 pKa = 9.88 RR98 pKa = 11.84 RR99 pKa = 11.84 LRR101 pKa = 11.84 PWLYY105 pKa = 10.66 DD106 pKa = 3.11 GTPQKK111 pKa = 10.53 HH112 pKa = 6.08 SRR114 pKa = 11.84 DD115 pKa = 4.06 PYY117 pKa = 10.08 WFDD120 pKa = 3.01 VTYY123 pKa = 11.33 NQMFKK128 pKa = 10.24 KK129 pKa = 9.84 WSEE132 pKa = 3.95 AQQ134 pKa = 3.19
Molecular weight: 16.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.132
IPC2_protein 9.37
IPC_protein 9.385
Toseland 10.116
ProMoST 9.75
Dawson 10.292
Bjellqvist 9.94
Wikipedia 10.438
Rodwell 10.745
Grimsley 10.35
Solomon 10.306
Lehninger 10.277
Nozaki 10.087
DTASelect 9.926
Thurlkill 10.145
EMBOSS 10.511
Sillero 10.189
Patrickios 10.379
IPC_peptide 10.321
IPC2_peptide 8.463
IPC2.peptide.svr19 8.404
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
62
0
62
13657
46
2066
220.3
25.18
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.763 ± 0.577
0.498 ± 0.097
6.253 ± 0.299
7.835 ± 0.402
3.888 ± 0.214
6.077 ± 0.548
1.743 ± 0.114
7.139 ± 0.393
9.98 ± 0.449
8.245 ± 0.256
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.533 ± 0.119
6.268 ± 0.251
2.695 ± 0.198
3.661 ± 0.158
4.13 ± 0.209
6.48 ± 0.301
5.704 ± 0.223
5.492 ± 0.221
1.289 ± 0.133
4.327 ± 0.365
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here