Sphingobacterium wenxiniae
Average proteome isoelectric point is 6.44
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3412 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1I6Q3Y3|A0A1I6Q3Y3_9SPHI Starch-binding associating with outer membrane OS=Sphingobacterium wenxiniae OX=683125 GN=SAMN05660206_102103 PE=3 SV=1
MM1 pKa = 7.48 KK2 pKa = 10.11 KK3 pKa = 9.56 IRR5 pKa = 11.84 YY6 pKa = 8.81 ILISLHH12 pKa = 6.8 LVLLLLGGISSLHH25 pKa = 5.58 AQCVDD30 pKa = 3.24 NTGLIAFTGYY40 pKa = 10.55 QIADD44 pKa = 3.28 AGNPQGQSDD53 pKa = 3.92 RR54 pKa = 11.84 FSFVTLDD61 pKa = 3.9 SIPPNTPIRR70 pKa = 11.84 FTDD73 pKa = 3.09 RR74 pKa = 11.84 GFTGGSSFQDD84 pKa = 3.31 FGNALTDD91 pKa = 4.65 GDD93 pKa = 4.85 FSWSDD98 pKa = 3.27 ASGISPGTHH107 pKa = 4.49 IVVTLGSGTATSTIGSITGYY127 pKa = 10.4 LGNFNLGLSGDD138 pKa = 3.77 QIFAYY143 pKa = 8.62 TASPSFKK150 pKa = 9.61 IHH152 pKa = 6.73 AAMLINKK159 pKa = 7.29 SAWDD163 pKa = 3.76 TEE165 pKa = 4.05 MLEE168 pKa = 4.23 LGNPIVTSSKK178 pKa = 10.78 SLNPAIAPVRR188 pKa = 11.84 QSITVYY194 pKa = 10.96 NSTDD198 pKa = 3.23 DD199 pKa = 4.07 AFQAKK204 pKa = 9.85 LNPITLTGSRR214 pKa = 11.84 TALRR218 pKa = 11.84 NQLFAGIFNIANSLNTDD235 pKa = 3.19 AVNLSVSPLTWNASVPTYY253 pKa = 10.74 TPISLNNKK261 pKa = 8.59 ILSSTVTDD269 pKa = 2.99 ACYY272 pKa = 10.24 IQWQSSIDD280 pKa = 3.88 GINFSNINDD289 pKa = 3.56 GTAFSGTKK297 pKa = 8.68 TASLTINTPLVADD310 pKa = 3.77 TWFRR314 pKa = 11.84 MHH316 pKa = 6.74 LTGAAYY322 pKa = 8.48 TVTDD326 pKa = 5.33 AIKK329 pKa = 9.35 LTLPAVTAHH338 pKa = 6.77 PLNRR342 pKa = 11.84 SICVGEE348 pKa = 3.94 NTSFSVTATGTDD360 pKa = 3.66 LQYY363 pKa = 11.02 QWQEE367 pKa = 3.73 DD368 pKa = 3.85 RR369 pKa = 11.84 GSGFTDD375 pKa = 3.89 LSDD378 pKa = 3.35 GGVYY382 pKa = 10.41 SGVTTSTLNISGATAGMDD400 pKa = 3.95 GYY402 pKa = 10.37 QYY404 pKa = 10.81 RR405 pKa = 11.84 VRR407 pKa = 11.84 VYY409 pKa = 10.91 SSIANPVTSNSATLSVSNIQATISKK434 pKa = 9.15 TDD436 pKa = 3.21 VGCFGEE442 pKa = 4.44 ASGSATVSNVMGGIGSYY459 pKa = 8.9 TYY461 pKa = 10.33 SWVRR465 pKa = 11.84 QGTTDD470 pKa = 3.92 IISDD474 pKa = 3.85 TEE476 pKa = 4.23 TASGLTEE483 pKa = 3.7 GTYY486 pKa = 10.1 TVTIRR491 pKa = 11.84 DD492 pKa = 3.72 AIGCVKK498 pKa = 9.68 TLDD501 pKa = 4.03 VIIMQPSALTASTTQTNVSCFNGSNGTATVSVSGGTGPYY540 pKa = 8.7 TYY542 pKa = 10.73 SWLPSGGTSQTATGLSAGTYY562 pKa = 7.78 TVTVTDD568 pKa = 4.67 ANSCQITRR576 pKa = 11.84 SFTITHH582 pKa = 7.15 PPALMASDD590 pKa = 3.96 GGKK593 pKa = 8.73 TNVSCNGGSNGTATVNVVGGTGAYY617 pKa = 8.11 TYY619 pKa = 9.18 TYY621 pKa = 9.87 SWAPFGGTSQTATGLSAGLYY641 pKa = 8.09 TVTVTDD647 pKa = 4.59 ANGCQTTQNFEE658 pKa = 3.77 ISEE661 pKa = 4.22 PTSAVVITTSDD672 pKa = 2.93 IMHH675 pKa = 6.66 VSTVGGSDD683 pKa = 3.21 GSATVDD689 pKa = 3.36 VMGGTAPYY697 pKa = 8.8 TYY699 pKa = 10.06 SWSPTGGTGATATGLSAGVYY719 pKa = 8.4 TVTVTDD725 pKa = 5.15 ANTCTYY731 pKa = 9.51 SHH733 pKa = 6.68 NVTIEE738 pKa = 3.91 QQAVNQAPVISNLNGDD754 pKa = 3.63 AVTFTEE760 pKa = 5.2 DD761 pKa = 3.25 GPAVLLDD768 pKa = 3.45 VFGNATVSDD777 pKa = 4.37 PDD779 pKa = 3.74 SPDD782 pKa = 3.38 FNEE785 pKa = 4.23 GNLTVSIVTNGVAAEE800 pKa = 4.0 DD801 pKa = 3.6 RR802 pKa = 11.84 LGIRR806 pKa = 11.84 HH807 pKa = 6.3 EE808 pKa = 4.5 GSGPGLIDD816 pKa = 3.31 VAGGDD821 pKa = 3.8 VYY823 pKa = 11.64 YY824 pKa = 10.75 GGTPIGSLTGGIAGQDD840 pKa = 3.25 LVVTFLPNSTPAAAQALIRR859 pKa = 11.84 NLTYY863 pKa = 11.39 SNVNTGNPSSAPRR876 pKa = 11.84 TVYY879 pKa = 8.89 ITLSDD884 pKa = 3.78 GDD886 pKa = 4.25 GGTSAVQAVTVSIKK900 pKa = 9.55 TVNDD904 pKa = 3.28 APTISVPVQINVTEE918 pKa = 4.25 DD919 pKa = 3.35 VPVALTGITFYY930 pKa = 11.22 DD931 pKa = 3.45 VDD933 pKa = 3.92 NTDD936 pKa = 2.92 VTVTFRR942 pKa = 11.84 TTIAGAGSGTLTATSTADD960 pKa = 3.33 VTVSGSGTNEE970 pKa = 3.43 LTMAGPWASINNLIAANGLVFLTDD994 pKa = 3.32 QDD996 pKa = 3.8 NVNTVFLNIQVSDD1009 pKa = 3.83 GALADD1014 pKa = 3.73 STATSLIVAAVNDD1027 pKa = 4.09 APQISVPLLQTVLQDD1042 pKa = 3.25 ATLTFNAANGNPISISDD1059 pKa = 3.58 VDD1061 pKa = 4.34 AGTNPIKK1068 pKa = 11.01 LNLTTTRR1075 pKa = 11.84 GLLSLGSTNGLSFDD1089 pKa = 4.28 IGNGTADD1096 pKa = 3.18 NTMEE1100 pKa = 4.25 FTGTLADD1107 pKa = 4.31 INAALNGLAFEE1118 pKa = 4.55 PTGGYY1123 pKa = 9.51 YY1124 pKa = 10.57 GPAAINITADD1134 pKa = 3.51 DD1135 pKa = 3.77 QGATGAGGPKK1145 pKa = 9.87 SVTRR1149 pKa = 11.84 EE1150 pKa = 3.47 IPITVEE1156 pKa = 3.45 PLNPKK1161 pKa = 7.98 VTSVSASTADD1171 pKa = 3.65 GLYY1174 pKa = 10.64 KK1175 pKa = 10.38 ADD1177 pKa = 4.61 DD1178 pKa = 4.59 VITLTVTFNQNVVVNTNGGDD1198 pKa = 3.57 TPTLLLEE1205 pKa = 4.11 TGIIDD1210 pKa = 4.16 RR1211 pKa = 11.84 SAIYY1215 pKa = 9.77 MGGSGSNTLTFIYY1228 pKa = 9.41 TVQPGDD1234 pKa = 3.3 VSADD1238 pKa = 3.35 LDD1240 pKa = 4.13 YY1241 pKa = 11.8 VSISALSLNGATILSSYY1258 pKa = 10.51 NGANAVLTLPTVGGASSIAGQKK1280 pKa = 10.51 NIVIDD1285 pKa = 4.54 GIVPVVNAVAVPANAYY1301 pKa = 9.63 YY1302 pKa = 10.94 GSGDD1306 pKa = 3.3 QLYY1309 pKa = 7.98 FTVEE1313 pKa = 3.92 FSEE1316 pKa = 4.38 AVAVNTTGGSPALSLTIGSQTVSAQYY1342 pKa = 9.05 LTEE1345 pKa = 4.36 SSTTTDD1351 pKa = 3.19 LLFRR1355 pKa = 11.84 YY1356 pKa = 9.27 SVAAGMQDD1364 pKa = 2.72 HH1365 pKa = 7.46 DD1366 pKa = 4.9 GIEE1369 pKa = 4.07 IGTLSLNGATIRR1381 pKa = 11.84 DD1382 pKa = 3.65 AAGNDD1387 pKa = 3.48 AVLTLNNVGATTHH1400 pKa = 5.63 VKK1402 pKa = 10.04 VDD1404 pKa = 3.36 AKK1406 pKa = 11.08 SPVVTMTINGGATHH1420 pKa = 6.53 TNDD1423 pKa = 3.65 RR1424 pKa = 11.84 NVTLTLTTADD1434 pKa = 3.33 TDD1436 pKa = 3.87 VATMSFMNSPIFFPGSGASQPEE1458 pKa = 4.03 PFAPTKK1464 pKa = 10.3 AWQLTAGDD1472 pKa = 3.69 GMKK1475 pKa = 9.97 FVSGIVIDD1483 pKa = 4.04 GAGNLTSVLAVITLDD1498 pKa = 3.1 QTAPIVTGVDD1508 pKa = 2.82 EE1509 pKa = 4.88 GEE1511 pKa = 4.48 SYY1513 pKa = 9.37 NTDD1516 pKa = 2.8 RR1517 pKa = 11.84 TITFNEE1523 pKa = 4.26 GTATLNGATFISGTTVSQDD1542 pKa = 2.77 GAYY1545 pKa = 9.12 TLIVTDD1551 pKa = 3.64 EE1552 pKa = 4.75 AGNSTTLSFAIDD1564 pKa = 3.35 QTAPAVPAGLTATPDD1579 pKa = 3.14 AGNINLQWTANQEE1592 pKa = 3.93 ADD1594 pKa = 3.18 LAAYY1598 pKa = 10.09 RR1599 pKa = 11.84 LYY1601 pKa = 11.0 SGTAADD1607 pKa = 4.0 QLSVLADD1614 pKa = 3.65 IPAGTTTYY1622 pKa = 9.45 THH1624 pKa = 6.96 GPLTMGQRR1632 pKa = 11.84 YY1633 pKa = 9.05 YY1634 pKa = 11.24 YY1635 pKa = 10.52 AITAIDD1641 pKa = 3.5 ALGNEE1646 pKa = 4.75 SAQSVVVDD1654 pKa = 5.08 ALPQDD1659 pKa = 3.76 TQTITFGTLNDD1670 pKa = 3.75 MTYY1673 pKa = 11.38 GDD1675 pKa = 4.77 APFALTATTTSGLTVAYY1692 pKa = 9.92 SSSDD1696 pKa = 3.26 NSIASIAGDD1705 pKa = 3.51 VLTIHH1710 pKa = 6.18 QAGDD1714 pKa = 3.54 VVITASQNGNNAFLAATPVTQALHH1738 pKa = 5.9 INKK1741 pKa = 9.57 ADD1743 pKa = 3.68 FAGITFDD1750 pKa = 5.28 DD1751 pKa = 3.86 KK1752 pKa = 11.26 TVTYY1756 pKa = 10.19 DD1757 pKa = 3.76 GSAHH1761 pKa = 6.8 SIFVSNAPTDD1771 pKa = 3.39 ATVIYY1776 pKa = 9.97 TNNNQINAGTYY1787 pKa = 7.96 TVMATVSQANYY1798 pKa = 10.5 NDD1800 pKa = 4.14 LPLTAEE1806 pKa = 4.12 LTIEE1810 pKa = 4.16 KK1811 pKa = 10.35 ADD1813 pKa = 3.72 FAGITFNDD1821 pKa = 3.25 ATVTYY1826 pKa = 9.67 DD1827 pKa = 3.8 GSAHH1831 pKa = 6.51 SIAVANTPTGATITYY1846 pKa = 9.54 VGNAQINAGTYY1857 pKa = 6.69 TVRR1860 pKa = 11.84 ATVSQANYY1868 pKa = 10.1 NDD1870 pKa = 3.87 LVLTAEE1876 pKa = 4.31 LEE1878 pKa = 4.07 ITKK1881 pKa = 10.97 AMLTDD1886 pKa = 3.23 IALRR1890 pKa = 11.84 NNSFVYY1896 pKa = 10.57 DD1897 pKa = 4.04 GNAKK1901 pKa = 10.08 SLEE1904 pKa = 3.95 ISGILPTGVQVIYY1917 pKa = 10.17 TGNNQVNAGMYY1928 pKa = 8.74 TVVAEE1933 pKa = 4.36 IADD1936 pKa = 4.34 TQNHH1940 pKa = 5.42 TGLRR1944 pKa = 11.84 LEE1946 pKa = 4.73 AQLQIDD1952 pKa = 4.62 KK1953 pKa = 10.8 APQTITFTSPGMLGRR1968 pKa = 11.84 DD1969 pKa = 3.64 AGTVALDD1976 pKa = 3.33 VRR1978 pKa = 11.84 SDD1980 pKa = 3.24 SGLPVSLSVDD1990 pKa = 3.27 DD1991 pKa = 5.43 DD1992 pKa = 3.78 MVGRR1996 pKa = 11.84 IEE1998 pKa = 4.17 GLNLEE2003 pKa = 4.29 ILRR2006 pKa = 11.84 LGTVTVTAIQPGNGNYY2022 pKa = 8.47 EE2023 pKa = 3.79 AAEE2026 pKa = 3.97 PVSFSVRR2033 pKa = 11.84 VANDD2037 pKa = 2.79 ATAAVPIRR2045 pKa = 11.84 VHH2047 pKa = 5.9 QAVSPNGDD2055 pKa = 3.81 GINDD2059 pKa = 3.82 FLMMEE2064 pKa = 5.35 GILDD2068 pKa = 3.73 YY2069 pKa = 11.02 PEE2071 pKa = 4.26 NKK2073 pKa = 8.16 VTIFDD2078 pKa = 3.82 KK2079 pKa = 10.61 SGKK2082 pKa = 9.83 VIDD2085 pKa = 5.03 VIEE2088 pKa = 4.52 SYY2090 pKa = 11.44 NNRR2093 pKa = 11.84 DD2094 pKa = 3.13 RR2095 pKa = 11.84 VFTGQFVNDD2104 pKa = 3.69 GTYY2107 pKa = 10.64 YY2108 pKa = 10.88 YY2109 pKa = 10.78 YY2110 pKa = 10.15 IDD2112 pKa = 3.96 VKK2114 pKa = 11.23 DD2115 pKa = 3.51 GGVWKK2120 pKa = 10.22 RR2121 pKa = 11.84 EE2122 pKa = 3.58 KK2123 pKa = 11.03 GFFVVKK2129 pKa = 10.55 RR2130 pKa = 11.84 SVNN2133 pKa = 3.26
Molecular weight: 220.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.78
IPC2_protein 3.999
IPC_protein 4.05
Toseland 3.821
ProMoST 4.215
Dawson 4.05
Bjellqvist 4.202
Wikipedia 3.999
Rodwell 3.872
Grimsley 3.732
Solomon 4.05
Lehninger 4.012
Nozaki 4.151
DTASelect 4.457
Thurlkill 3.872
EMBOSS 4.012
Sillero 4.177
Patrickios 1.354
IPC_peptide 4.05
IPC2_peptide 4.151
IPC2.peptide.svr19 4.033
Protein with the highest isoelectric point:
>tr|A0A1I6NV61|A0A1I6NV61_9SPHI De-hypoxanthine futalosine cyclase OS=Sphingobacterium wenxiniae OX=683125 GN=SAMN05660206_101117 PE=4 SV=1
MM1 pKa = 7.29 YY2 pKa = 10.36 NGSLVMSFRR11 pKa = 11.84 PLVMNIGSLVMSIRR25 pKa = 11.84 SLVMSIRR32 pKa = 11.84 PLVMSIRR39 pKa = 11.84 PLVMSFRR46 pKa = 11.84 LLVMSFRR53 pKa = 11.84 PLVMSIRR60 pKa = 11.84 SLVMSIRR67 pKa = 11.84 SLVMSIRR74 pKa = 11.84 LLVMSIRR81 pKa = 11.84 PLVMSFRR88 pKa = 11.84 PLVMNIGSLVMNIRR102 pKa = 11.84 PLLMHH107 pKa = 6.63 IGKK110 pKa = 8.87 WLLTEE115 pKa = 4.15 KK116 pKa = 10.9
Molecular weight: 13.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.343
IPC2_protein 10.877
IPC_protein 12.325
Toseland 12.486
ProMoST 12.983
Dawson 12.486
Bjellqvist 12.486
Wikipedia 12.954
Rodwell 12.062
Grimsley 12.515
Solomon 12.983
Lehninger 12.881
Nozaki 12.486
DTASelect 12.486
Thurlkill 12.486
EMBOSS 12.983
Sillero 12.486
Patrickios 11.798
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.135
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3412
0
3412
1141595
14
3684
334.6
37.72
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.082 ± 0.043
0.739 ± 0.014
5.482 ± 0.029
6.483 ± 0.045
4.912 ± 0.032
6.709 ± 0.043
1.982 ± 0.022
7.266 ± 0.038
6.704 ± 0.045
9.51 ± 0.041
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.32 ± 0.019
5.317 ± 0.04
3.565 ± 0.021
4.019 ± 0.027
4.232 ± 0.028
6.201 ± 0.03
5.581 ± 0.043
6.529 ± 0.035
1.155 ± 0.017
4.213 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here