Amino acid dipepetide frequency for Bacillus phage Deep Blue

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.603AlaAla: 2.603 ± 0.31
0.384AlaCys: 0.384 ± 0.088
3.393AlaAsp: 3.393 ± 0.299
4.545AlaGlu: 4.545 ± 0.359
2.539AlaPhe: 2.539 ± 0.239
4.823AlaGly: 4.823 ± 0.434
1.046AlaHis: 1.046 ± 0.152
4.588AlaIle: 4.588 ± 0.319
4.93AlaLys: 4.93 ± 0.377
4.887AlaLeu: 4.887 ± 0.36
1.707AlaMet: 1.707 ± 0.21
3.158AlaAsn: 3.158 ± 0.357
1.878AlaPro: 1.878 ± 0.312
2.433AlaGln: 2.433 ± 0.233
2.475AlaArg: 2.475 ± 0.291
3.372AlaSer: 3.372 ± 0.366
4.417AlaThr: 4.417 ± 0.328
3.777AlaVal: 3.777 ± 0.243
0.832AlaTrp: 0.832 ± 0.137
2.86AlaTyr: 2.86 ± 0.264
0.0AlaXaa: 0.0 ± 0.0
Cys
0.277CysAla: 0.277 ± 0.085
0.085CysCys: 0.085 ± 0.045
0.576CysAsp: 0.576 ± 0.121
0.491CysGlu: 0.491 ± 0.089
0.32CysPhe: 0.32 ± 0.082
0.662CysGly: 0.662 ± 0.133
0.256CysHis: 0.256 ± 0.067
0.448CysIle: 0.448 ± 0.112
0.619CysLys: 0.619 ± 0.124
0.427CysLeu: 0.427 ± 0.094
0.149CysMet: 0.149 ± 0.062
0.384CysAsn: 0.384 ± 0.085
0.256CysPro: 0.256 ± 0.076
0.149CysGln: 0.149 ± 0.058
0.235CysArg: 0.235 ± 0.069
0.555CysSer: 0.555 ± 0.109
0.405CysThr: 0.405 ± 0.091
0.534CysVal: 0.534 ± 0.108
0.107CysTrp: 0.107 ± 0.054
0.469CysTyr: 0.469 ± 0.119
0.0CysXaa: 0.0 ± 0.0
Asp
3.244AspAla: 3.244 ± 0.29
0.512AspCys: 0.512 ± 0.113
3.414AspAsp: 3.414 ± 0.328
4.545AspGlu: 4.545 ± 0.322
2.646AspPhe: 2.646 ± 0.246
4.332AspGly: 4.332 ± 0.373
0.918AspHis: 0.918 ± 0.144
4.93AspIle: 4.93 ± 0.304
5.548AspLys: 5.548 ± 0.373
5.207AspLeu: 5.207 ± 0.335
1.43AspMet: 1.43 ± 0.172
3.478AspAsn: 3.478 ± 0.289
2.262AspPro: 2.262 ± 0.232
1.387AspGln: 1.387 ± 0.193
2.646AspArg: 2.646 ± 0.259
3.542AspSer: 3.542 ± 0.222
3.457AspThr: 3.457 ± 0.295
4.183AspVal: 4.183 ± 0.317
0.982AspTrp: 0.982 ± 0.14
3.457AspTyr: 3.457 ± 0.282
0.0AspXaa: 0.0 ± 0.0
Glu
4.119GluAla: 4.119 ± 0.362
0.512GluCys: 0.512 ± 0.109
5.271GluAsp: 5.271 ± 0.367
8.493GluGlu: 8.493 ± 0.648
3.158GluPhe: 3.158 ± 0.277
4.759GluGly: 4.759 ± 0.336
1.536GluHis: 1.536 ± 0.185
5.164GluIle: 5.164 ± 0.357
5.933GluLys: 5.933 ± 0.467
7.704GluLeu: 7.704 ± 0.457
2.582GluMet: 2.582 ± 0.252
3.777GluAsn: 3.777 ± 0.29
1.899GluPro: 1.899 ± 0.283
3.671GluGln: 3.671 ± 0.299
3.436GluArg: 3.436 ± 0.3
3.094GluSer: 3.094 ± 0.255
3.457GluThr: 3.457 ± 0.265
5.933GluVal: 5.933 ± 0.486
0.96GluTrp: 0.96 ± 0.142
3.457GluTyr: 3.457 ± 0.234
0.0GluXaa: 0.0 ± 0.0
Phe
2.027PheAla: 2.027 ± 0.185
0.32PheCys: 0.32 ± 0.084
2.668PheAsp: 2.668 ± 0.235
3.265PheGlu: 3.265 ± 0.265
1.451PhePhe: 1.451 ± 0.169
2.283PheGly: 2.283 ± 0.224
0.832PheHis: 0.832 ± 0.142
2.454PheIle: 2.454 ± 0.232
2.924PheLys: 2.924 ± 0.249
2.753PheLeu: 2.753 ± 0.259
1.302PheMet: 1.302 ± 0.174
2.603PheAsn: 2.603 ± 0.307
1.131PhePro: 1.131 ± 0.154
1.366PheGln: 1.366 ± 0.174
1.494PheArg: 1.494 ± 0.164
2.902PheSer: 2.902 ± 0.269
2.625PheThr: 2.625 ± 0.278
2.881PheVal: 2.881 ± 0.273
0.405PheTrp: 0.405 ± 0.097
1.857PheTyr: 1.857 ± 0.207
0.0PheXaa: 0.0 ± 0.0
Gly
4.097GlyAla: 4.097 ± 0.462
0.555GlyCys: 0.555 ± 0.121
3.649GlyAsp: 3.649 ± 0.282
4.289GlyGlu: 4.289 ± 0.343
2.369GlyPhe: 2.369 ± 0.253
6.082GlyGly: 6.082 ± 0.865
1.195GlyHis: 1.195 ± 0.159
3.969GlyIle: 3.969 ± 0.293
4.972GlyLys: 4.972 ± 0.329
4.93GlyLeu: 4.93 ± 0.347
2.006GlyMet: 2.006 ± 0.271
3.777GlyAsn: 3.777 ± 0.309
0.128GlyPro: 0.128 ± 0.07
2.838GlyGln: 2.838 ± 0.288
2.539GlyArg: 2.539 ± 0.232
5.164GlySer: 5.164 ± 0.416
5.399GlyThr: 5.399 ± 0.469
5.058GlyVal: 5.058 ± 0.296
0.96GlyTrp: 0.96 ± 0.148
3.308GlyTyr: 3.308 ± 0.226
0.0GlyXaa: 0.0 ± 0.0
His
1.088HisAla: 1.088 ± 0.134
0.128HisCys: 0.128 ± 0.053
0.832HisAsp: 0.832 ± 0.125
1.451HisGlu: 1.451 ± 0.176
0.747HisPhe: 0.747 ± 0.139
0.918HisGly: 0.918 ± 0.158
0.448HisHis: 0.448 ± 0.116
1.387HisIle: 1.387 ± 0.187
1.174HisLys: 1.174 ± 0.187
1.451HisLeu: 1.451 ± 0.178
0.512HisMet: 0.512 ± 0.091
0.918HisAsn: 0.918 ± 0.136
0.64HisPro: 0.64 ± 0.111
0.469HisGln: 0.469 ± 0.101
1.195HisArg: 1.195 ± 0.166
0.768HisSer: 0.768 ± 0.134
1.152HisThr: 1.152 ± 0.163
1.643HisVal: 1.643 ± 0.232
0.277HisTrp: 0.277 ± 0.086
0.619HisTyr: 0.619 ± 0.147
0.0HisXaa: 0.0 ± 0.0
Ile
4.46IleAla: 4.46 ± 0.315
0.363IleCys: 0.363 ± 0.096
4.055IleAsp: 4.055 ± 0.336
5.527IleGlu: 5.527 ± 0.378
1.771IlePhe: 1.771 ± 0.192
4.204IleGly: 4.204 ± 0.338
1.259IleHis: 1.259 ± 0.185
3.457IleIle: 3.457 ± 0.357
5.143IleLys: 5.143 ± 0.352
4.268IleLeu: 4.268 ± 0.277
1.536IleMet: 1.536 ± 0.197
3.905IleAsn: 3.905 ± 0.285
2.475IlePro: 2.475 ± 0.233
2.134IleGln: 2.134 ± 0.162
2.796IleArg: 2.796 ± 0.266
4.14IleSer: 4.14 ± 0.365
4.652IleThr: 4.652 ± 0.417
4.631IleVal: 4.631 ± 0.367
0.448IleTrp: 0.448 ± 0.086
2.305IleTyr: 2.305 ± 0.192
0.0IleXaa: 0.0 ± 0.0
Lys
5.015LysAla: 5.015 ± 0.323
0.405LysCys: 0.405 ± 0.107
5.484LysAsp: 5.484 ± 0.323
7.832LysGlu: 7.832 ± 0.557
2.902LysPhe: 2.902 ± 0.285
4.46LysGly: 4.46 ± 0.35
1.259LysHis: 1.259 ± 0.189
4.268LysIle: 4.268 ± 0.255
6.487LysLys: 6.487 ± 0.439
6.21LysLeu: 6.21 ± 0.333
2.326LysMet: 2.326 ± 0.237
3.286LysAsn: 3.286 ± 0.266
2.817LysPro: 2.817 ± 0.285
3.457LysGln: 3.457 ± 0.297
3.5LysArg: 3.5 ± 0.336
3.884LysSer: 3.884 ± 0.355
4.14LysThr: 4.14 ± 0.283
5.826LysVal: 5.826 ± 0.383
0.96LysTrp: 0.96 ± 0.159
3.116LysTyr: 3.116 ± 0.285
0.0LysXaa: 0.0 ± 0.0
Leu
5.314LeuAla: 5.314 ± 0.351
0.598LeuCys: 0.598 ± 0.106
5.207LeuAsp: 5.207 ± 0.344
6.658LeuGlu: 6.658 ± 0.418
3.606LeuPhe: 3.606 ± 0.259
5.591LeuGly: 5.591 ± 0.38
1.302LeuHis: 1.302 ± 0.161
4.588LeuIle: 4.588 ± 0.369
5.783LeuLys: 5.783 ± 0.361
6.445LeuLeu: 6.445 ± 0.449
1.942LeuMet: 1.942 ± 0.234
4.396LeuAsn: 4.396 ± 0.326
2.603LeuPro: 2.603 ± 0.262
3.414LeuGln: 3.414 ± 0.247
3.713LeuArg: 3.713 ± 0.374
4.567LeuSer: 4.567 ± 0.336
5.399LeuThr: 5.399 ± 0.348
5.378LeuVal: 5.378 ± 0.329
0.768LeuTrp: 0.768 ± 0.132
3.329LeuTyr: 3.329 ± 0.254
0.0LeuXaa: 0.0 ± 0.0
Met
2.113MetAla: 2.113 ± 0.195
0.128MetCys: 0.128 ± 0.043
2.006MetAsp: 2.006 ± 0.2
2.049MetGlu: 2.049 ± 0.193
1.088MetPhe: 1.088 ± 0.154
1.28MetGly: 1.28 ± 0.182
0.341MetHis: 0.341 ± 0.08
1.558MetIle: 1.558 ± 0.198
2.689MetLys: 2.689 ± 0.227
2.113MetLeu: 2.113 ± 0.197
0.747MetMet: 0.747 ± 0.141
1.75MetAsn: 1.75 ± 0.205
0.832MetPro: 0.832 ± 0.205
0.982MetGln: 0.982 ± 0.144
1.174MetArg: 1.174 ± 0.159
1.835MetSer: 1.835 ± 0.196
1.793MetThr: 1.793 ± 0.215
1.515MetVal: 1.515 ± 0.209
0.363MetTrp: 0.363 ± 0.09
0.896MetTyr: 0.896 ± 0.13
0.0MetXaa: 0.0 ± 0.0
Asn
3.116AsnAla: 3.116 ± 0.271
0.491AsnCys: 0.491 ± 0.116
2.582AsnAsp: 2.582 ± 0.256
3.414AsnGlu: 3.414 ± 0.265
2.049AsnPhe: 2.049 ± 0.224
4.076AsnGly: 4.076 ± 0.414
1.003AsnHis: 1.003 ± 0.134
3.628AsnIle: 3.628 ± 0.294
4.204AsnLys: 4.204 ± 0.303
4.119AsnLeu: 4.119 ± 0.272
1.707AsnMet: 1.707 ± 0.177
3.052AsnAsn: 3.052 ± 0.319
2.326AsnPro: 2.326 ± 0.267
1.857AsnGln: 1.857 ± 0.189
2.411AsnArg: 2.411 ± 0.233
2.625AsnSer: 2.625 ± 0.223
3.671AsnThr: 3.671 ± 0.329
3.948AsnVal: 3.948 ± 0.324
0.662AsnTrp: 0.662 ± 0.124
2.091AsnTyr: 2.091 ± 0.209
0.0AsnXaa: 0.0 ± 0.0
Pro
1.643ProAla: 1.643 ± 0.223
0.235ProCys: 0.235 ± 0.075
1.835ProAsp: 1.835 ± 0.232
2.07ProGlu: 2.07 ± 0.238
1.408ProPhe: 1.408 ± 0.208
1.729ProGly: 1.729 ± 0.236
0.662ProHis: 0.662 ± 0.128
1.899ProIle: 1.899 ± 0.196
2.988ProLys: 2.988 ± 0.292
2.475ProLeu: 2.475 ± 0.233
0.939ProMet: 0.939 ± 0.165
1.601ProAsn: 1.601 ± 0.21
0.79ProPro: 0.79 ± 0.132
1.302ProGln: 1.302 ± 0.191
1.216ProArg: 1.216 ± 0.178
1.857ProSer: 1.857 ± 0.219
2.603ProThr: 2.603 ± 0.422
2.454ProVal: 2.454 ± 0.249
0.277ProTrp: 0.277 ± 0.097
1.366ProTyr: 1.366 ± 0.169
0.0ProXaa: 0.0 ± 0.0
Gln
2.924GlnAla: 2.924 ± 0.277
0.384GlnCys: 0.384 ± 0.09
2.155GlnAsp: 2.155 ± 0.205
3.5GlnGlu: 3.5 ± 0.279
1.216GlnPhe: 1.216 ± 0.137
2.582GlnGly: 2.582 ± 0.259
0.832GlnHis: 0.832 ± 0.138
2.113GlnIle: 2.113 ± 0.24
2.475GlnLys: 2.475 ± 0.257
3.478GlnLeu: 3.478 ± 0.314
0.96GlnMet: 0.96 ± 0.159
1.707GlnAsn: 1.707 ± 0.149
1.451GlnPro: 1.451 ± 0.299
1.793GlnGln: 1.793 ± 0.236
1.601GlnArg: 1.601 ± 0.178
2.177GlnSer: 2.177 ± 0.213
2.027GlnThr: 2.027 ± 0.211
2.817GlnVal: 2.817 ± 0.25
0.491GlnTrp: 0.491 ± 0.107
1.366GlnTyr: 1.366 ± 0.184
0.0GlnXaa: 0.0 ± 0.0
Arg
2.411ArgAla: 2.411 ± 0.286
0.32ArgCys: 0.32 ± 0.074
2.475ArgAsp: 2.475 ± 0.223
3.606ArgGlu: 3.606 ± 0.261
1.793ArgPhe: 1.793 ± 0.188
2.945ArgGly: 2.945 ± 0.276
0.512ArgHis: 0.512 ± 0.101
2.924ArgIle: 2.924 ± 0.228
3.18ArgLys: 3.18 ± 0.295
3.542ArgLeu: 3.542 ± 0.315
1.43ArgMet: 1.43 ± 0.16
2.091ArgAsn: 2.091 ± 0.207
1.216ArgPro: 1.216 ± 0.173
1.536ArgGln: 1.536 ± 0.18
2.027ArgArg: 2.027 ± 0.245
2.347ArgSer: 2.347 ± 0.235
2.561ArgThr: 2.561 ± 0.234
3.329ArgVal: 3.329 ± 0.273
0.469ArgTrp: 0.469 ± 0.106
1.921ArgTyr: 1.921 ± 0.216
0.0ArgXaa: 0.0 ± 0.0
Ser
3.18SerAla: 3.18 ± 0.227
0.32SerCys: 0.32 ± 0.094
3.777SerAsp: 3.777 ± 0.323
3.457SerGlu: 3.457 ± 0.257
2.945SerPhe: 2.945 ± 0.292
4.204SerGly: 4.204 ± 0.37
0.832SerHis: 0.832 ± 0.119
3.393SerIle: 3.393 ± 0.265
4.161SerLys: 4.161 ± 0.249
4.972SerLeu: 4.972 ± 0.355
1.472SerMet: 1.472 ± 0.182
2.753SerAsn: 2.753 ± 0.258
1.771SerPro: 1.771 ± 0.163
2.177SerGln: 2.177 ± 0.228
2.561SerArg: 2.561 ± 0.229
4.375SerSer: 4.375 ± 0.604
4.161SerThr: 4.161 ± 0.332
4.332SerVal: 4.332 ± 0.329
0.726SerTrp: 0.726 ± 0.168
2.753SerTyr: 2.753 ± 0.236
0.0SerXaa: 0.0 ± 0.0
Thr
4.353ThrAla: 4.353 ± 0.463
0.405ThrCys: 0.405 ± 0.096
3.969ThrAsp: 3.969 ± 0.298
4.353ThrGlu: 4.353 ± 0.363
2.902ThrPhe: 2.902 ± 0.263
4.545ThrGly: 4.545 ± 0.336
1.11ThrHis: 1.11 ± 0.15
4.908ThrIle: 4.908 ± 0.354
4.055ThrLys: 4.055 ± 0.271
5.186ThrLeu: 5.186 ± 0.38
1.238ThrMet: 1.238 ± 0.15
3.286ThrAsn: 3.286 ± 0.37
2.945ThrPro: 2.945 ± 0.309
2.134ThrGln: 2.134 ± 0.19
2.603ThrArg: 2.603 ± 0.276
3.436ThrSer: 3.436 ± 0.286
3.756ThrThr: 3.756 ± 0.349
5.975ThrVal: 5.975 ± 0.383
0.662ThrTrp: 0.662 ± 0.121
2.988ThrTyr: 2.988 ± 0.234
0.0ThrXaa: 0.0 ± 0.0
Val
5.015ValAla: 5.015 ± 0.335
0.534ValCys: 0.534 ± 0.093
4.609ValAsp: 4.609 ± 0.35
5.548ValGlu: 5.548 ± 0.419
2.561ValPhe: 2.561 ± 0.239
4.204ValGly: 4.204 ± 0.335
1.259ValHis: 1.259 ± 0.167
4.332ValIle: 4.332 ± 0.309
5.762ValLys: 5.762 ± 0.37
5.911ValLeu: 5.911 ± 0.364
1.985ValMet: 1.985 ± 0.196
3.777ValAsn: 3.777 ± 0.337
2.625ValPro: 2.625 ± 0.258
2.796ValGln: 2.796 ± 0.232
3.094ValArg: 3.094 ± 0.273
4.524ValSer: 4.524 ± 0.315
5.228ValThr: 5.228 ± 0.337
5.57ValVal: 5.57 ± 0.399
1.003ValTrp: 1.003 ± 0.149
3.116ValTyr: 3.116 ± 0.248
0.0ValXaa: 0.0 ± 0.0
Trp
0.832TrpAla: 0.832 ± 0.145
0.213TrpCys: 0.213 ± 0.071
0.918TrpAsp: 0.918 ± 0.148
1.11TrpGlu: 1.11 ± 0.153
0.555TrpPhe: 0.555 ± 0.115
0.683TrpGly: 0.683 ± 0.129
0.171TrpHis: 0.171 ± 0.053
0.726TrpIle: 0.726 ± 0.124
0.918TrpLys: 0.918 ± 0.163
0.96TrpLeu: 0.96 ± 0.152
0.235TrpMet: 0.235 ± 0.084
0.747TrpAsn: 0.747 ± 0.164
0.0TrpPro: 0.0 ± 0.0
0.448TrpGln: 0.448 ± 0.108
0.448TrpArg: 0.448 ± 0.095
0.598TrpSer: 0.598 ± 0.109
0.811TrpThr: 0.811 ± 0.155
0.832TrpVal: 0.832 ± 0.156
0.213TrpTrp: 0.213 ± 0.067
0.555TrpTyr: 0.555 ± 0.097
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.817TyrAla: 2.817 ± 0.236
0.512TyrCys: 0.512 ± 0.1
3.244TyrAsp: 3.244 ± 0.225
2.732TyrGlu: 2.732 ± 0.242
1.515TyrPhe: 1.515 ± 0.188
2.924TyrGly: 2.924 ± 0.264
1.046TyrHis: 1.046 ± 0.162
2.796TyrIle: 2.796 ± 0.228
3.692TyrLys: 3.692 ± 0.29
3.564TyrLeu: 3.564 ± 0.25
1.024TyrMet: 1.024 ± 0.146
2.582TyrAsn: 2.582 ± 0.228
1.302TyrPro: 1.302 ± 0.172
1.707TyrGln: 1.707 ± 0.147
1.472TyrArg: 1.472 ± 0.18
2.561TyrSer: 2.561 ± 0.227
3.094TyrThr: 3.094 ± 0.246
2.838TyrVal: 2.838 ± 0.238
0.405TyrTrp: 0.405 ± 0.087
1.793TyrTyr: 1.793 ± 0.212
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 226 proteins (46861 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski