Mesoplasma corruscae
Average proteome isoelectric point is 7.27
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 701 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2S5RGV7|A0A2S5RGV7_9MOLU F0F1 ATP synthase subunit epsilon OS=Mesoplasma corruscae OX=216874 GN=atpC PE=3 SV=1
MM1 pKa = 7.63 NNVLDD6 pKa = 4.18 LNNYY10 pKa = 8.43 EE11 pKa = 4.44 FFNGIDD17 pKa = 3.93 PNNSLIQTDD26 pKa = 4.47 IEE28 pKa = 4.63 SFWISLKK35 pKa = 10.01 CTVICNIPRR44 pKa = 11.84 EE45 pKa = 4.44 SEE47 pKa = 3.88 KK48 pKa = 11.2 SEE50 pKa = 3.87
Molecular weight: 5.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.22
IPC2_protein 4.228
IPC_protein 3.986
Toseland 3.834
ProMoST 4.05
Dawson 3.961
Bjellqvist 4.24
Wikipedia 3.872
Rodwell 3.834
Grimsley 3.757
Solomon 3.923
Lehninger 3.884
Nozaki 4.101
DTASelect 4.19
Thurlkill 3.884
EMBOSS 3.884
Sillero 4.101
Patrickios 3.3
IPC_peptide 3.935
IPC2_peptide 4.088
IPC2.peptide.svr19 4.053
Protein with the highest isoelectric point:
>tr|A0A2S5RHA6|A0A2S5RHA6_9MOLU Peptidyl-tRNA hydrolase OS=Mesoplasma corruscae OX=216874 GN=pth PE=3 SV=1
MM1 pKa = 7.32 KK2 pKa = 9.47 RR3 pKa = 11.84 TWQPSKK9 pKa = 9.91 IKK11 pKa = 10.06 HH12 pKa = 5.46 SRR14 pKa = 11.84 VHH16 pKa = 6.2 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.31 NGRR28 pKa = 11.84 KK29 pKa = 9.01 IIKK32 pKa = 9.57 ARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.34 GRR39 pKa = 11.84 AKK41 pKa = 9.64 LTAA44 pKa = 4.21
Molecular weight: 5.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.494
IPC2_protein 11.125
IPC_protein 12.691
Toseland 12.866
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.676
Grimsley 12.896
Solomon 13.349
Lehninger 13.247
Nozaki 12.866
DTASelect 12.852
Thurlkill 12.866
EMBOSS 13.349
Sillero 12.866
Patrickios 12.398
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.084
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
701
0
701
249984
29
2889
356.6
40.66
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.099 ± 0.096
0.607 ± 0.025
5.385 ± 0.094
6.648 ± 0.092
5.351 ± 0.09
5.014 ± 0.091
1.283 ± 0.04
10.421 ± 0.098
10.132 ± 0.094
9.307 ± 0.075
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.089 ± 0.039
7.487 ± 0.111
2.437 ± 0.055
3.295 ± 0.043
2.595 ± 0.059
6.891 ± 0.091
5.348 ± 0.101
5.876 ± 0.074
1.031 ± 0.034
3.703 ± 0.058
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here