Arthrobacter phage Shoya

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 67 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G6XI33|A0A6G6XI33_9CAUD Major capsid protein OS=Arthrobacter phage Shoya OX=2704035 GN=5 PE=4 SV=1
MM1 pKa = 7.06THH3 pKa = 6.15YY4 pKa = 10.66CPAVTFAGRR13 pKa = 11.84FYY15 pKa = 11.21VDD17 pKa = 3.79PEE19 pKa = 4.11PPEE22 pKa = 4.1TCGTEE27 pKa = 4.02VEE29 pKa = 4.42EE30 pKa = 4.67PGYY33 pKa = 10.79CPAHH37 pKa = 6.45EE38 pKa = 4.37EE39 pKa = 4.14DD40 pKa = 4.46DD41 pKa = 4.98RR42 pKa = 11.84SDD44 pKa = 3.85EE45 pKa = 5.29LYY47 pKa = 10.76DD48 pKa = 4.07AYY50 pKa = 11.38LDD52 pKa = 3.65AKK54 pKa = 10.95YY55 pKa = 10.6EE56 pKa = 4.08EE57 pKa = 4.55NQLDD61 pKa = 3.7PWGDD65 pKa = 3.57DD66 pKa = 3.18

Molecular weight: 7.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G6XHZ0|A0A6G6XHZ0_9CAUD Uncharacterized protein OS=Arthrobacter phage Shoya OX=2704035 GN=51 PE=4 SV=1
MM1 pKa = 7.63TKK3 pKa = 10.04PKK5 pKa = 10.28QRR7 pKa = 11.84TAPEE11 pKa = 3.82PTHH14 pKa = 5.88TFRR17 pKa = 11.84AVWPIIPGHH26 pKa = 6.55GIAEE30 pKa = 4.15TDD32 pKa = 3.43AEE34 pKa = 5.09LIAQAIGDD42 pKa = 4.41LPNVAHH48 pKa = 7.12RR49 pKa = 11.84HH50 pKa = 4.09QARR53 pKa = 11.84IVGPPRR59 pKa = 11.84ACIADD64 pKa = 3.58GRR66 pKa = 11.84RR67 pKa = 11.84VPGSGGAHH75 pKa = 3.19QVVVIEE81 pKa = 4.54APAVAAAGRR90 pKa = 11.84GYY92 pKa = 10.56RR93 pKa = 11.84HH94 pKa = 5.58QQ95 pKa = 3.91

Molecular weight: 10.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

67

0

67

11825

39

1129

176.5

19.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.448 ± 0.426

0.744 ± 0.161

5.987 ± 0.274

5.852 ± 0.415

2.977 ± 0.301

8.431 ± 0.533

2.021 ± 0.204

4.507 ± 0.269

4.389 ± 0.251

7.391 ± 0.32

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.266 ± 0.176

3.121 ± 0.191

5.751 ± 0.242

3.865 ± 0.228

6.309 ± 0.478

5.328 ± 0.233

6.841 ± 0.419

7.383 ± 0.416

2.08 ± 0.164

2.309 ± 0.192
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski