Theileria orientalis strain Shintoku

Taxonomy: cellular organisms; Eukaryota; Sar; Alveolata; Apicomplexa; Aconoidasida; Piroplasmida; Theileriidae; Theileria; Theileria orientalis

Average proteome isoelectric point is 6.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3989 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|J4DQ86|J4DQ86_THEOR GPI mannosyltransferase 1 OS=Theileria orientalis strain Shintoku OX=869250 GN=TOT_040000395 PE=3 SV=1
MM1 pKa = 7.36IADD4 pKa = 4.64PGTTFNNLGATPMKK18 pKa = 10.75NPLTPYY24 pKa = 10.84VLFILINSLNMLDD37 pKa = 5.17FLDD40 pKa = 4.17PMMLFLLFCMSTLPAA55 pKa = 5.13

Molecular weight: 6.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|J4CDH2|J4CDH2_THEOR Uncharacterized protein OS=Theileria orientalis strain Shintoku OX=869250 GN=TOT_030000400 PE=4 SV=1
MM1 pKa = 7.13SHH3 pKa = 6.36GASRR7 pKa = 11.84YY8 pKa = 9.02KK9 pKa = 10.21KK10 pKa = 9.89AHH12 pKa = 6.23AKK14 pKa = 8.78MRR16 pKa = 11.84WKK18 pKa = 9.17WKK20 pKa = 9.51KK21 pKa = 10.24KK22 pKa = 7.88RR23 pKa = 11.84THH25 pKa = 5.93RR26 pKa = 11.84LQRR29 pKa = 11.84KK30 pKa = 6.33RR31 pKa = 11.84RR32 pKa = 11.84KK33 pKa = 8.28MRR35 pKa = 11.84QRR37 pKa = 11.84SRR39 pKa = 3.22

Molecular weight: 5.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3989

0

3989

2048270

12

7144

513.5

58.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.785 ± 0.036

1.657 ± 0.021

5.713 ± 0.023

6.869 ± 0.045

4.47 ± 0.031

5.147 ± 0.037

2.211 ± 0.015

5.737 ± 0.045

7.713 ± 0.035

9.641 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.283 ± 0.021

6.255 ± 0.031

3.763 ± 0.041

3.076 ± 0.026

4.813 ± 0.03

8.702 ± 0.044

5.59 ± 0.035

6.509 ± 0.034

0.823 ± 0.009

4.244 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski