Botrytis cinerea mitovirus 1
Average proteome isoelectric point is 8.71
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A5YRF7|A5YRF7_9VIRU RNA-dependent RNA polymerase OS=Botrytis cinerea mitovirus 1 OX=444193 GN=RDRP PE=4 SV=3
MM1 pKa = 7.27 KK2 pKa = 10.2 HH3 pKa = 6.41 LFRR6 pKa = 11.84 IKK8 pKa = 10.14 RR9 pKa = 11.84 KK10 pKa = 9.69 AVIKK14 pKa = 10.78 NSTTRR19 pKa = 11.84 LPDD22 pKa = 3.96 HH23 pKa = 6.8 LWLGYY28 pKa = 10.63 VKK30 pKa = 10.7 VLVWLYY36 pKa = 9.65 DD37 pKa = 3.18 IPSAHH42 pKa = 5.68 RR43 pKa = 11.84 TYY45 pKa = 10.8 FFEE48 pKa = 4.47 LLNRR52 pKa = 11.84 IQSLRR57 pKa = 11.84 RR58 pKa = 11.84 NSGDD62 pKa = 2.81 TWTVGYY68 pKa = 10.28 LKK70 pKa = 10.26 EE71 pKa = 4.06 SHH73 pKa = 6.64 RR74 pKa = 11.84 LSSHH78 pKa = 5.36 WLSGNPDD85 pKa = 3.21 VCSGSIRR92 pKa = 11.84 VSVRR96 pKa = 11.84 GLPLIIPGRR105 pKa = 11.84 LRR107 pKa = 11.84 NVIYY111 pKa = 10.55 SRR113 pKa = 11.84 NNINITRR120 pKa = 11.84 ALLTLLSTFRR130 pKa = 11.84 VMSATPKK137 pKa = 10.49 LKK139 pKa = 10.63 LSTITDD145 pKa = 3.73 PFNGVNEE152 pKa = 4.34 TLPSLEE158 pKa = 3.91 VSRR161 pKa = 11.84 ALKK164 pKa = 10.74 NILQGNKK171 pKa = 7.26 FHH173 pKa = 7.32 FKK175 pKa = 10.76 KK176 pKa = 10.62 EE177 pKa = 4.33 DD178 pKa = 3.09 IKK180 pKa = 11.45 DD181 pKa = 3.69 PYY183 pKa = 10.6 SVNRR187 pKa = 11.84 LMDD190 pKa = 3.2 ITKK193 pKa = 10.1 ADD195 pKa = 3.87 PNGGVSLRR203 pKa = 11.84 NVIIDD208 pKa = 3.97 AYY210 pKa = 10.95 ALMKK214 pKa = 10.0 HH215 pKa = 5.83 PKK217 pKa = 9.69 VYY219 pKa = 10.89 ASLLEE224 pKa = 4.2 LSNVMSPYY232 pKa = 10.47 LGSLLRR238 pKa = 11.84 NQTTIIRR245 pKa = 11.84 AIIDD249 pKa = 3.51 YY250 pKa = 10.87 DD251 pKa = 4.11 PIEE254 pKa = 4.1 WEE256 pKa = 4.05 RR257 pKa = 11.84 IKK259 pKa = 11.07 PFMAPIYY266 pKa = 10.46 QNIKK270 pKa = 10.69 DD271 pKa = 3.84 RR272 pKa = 11.84 TLKK275 pKa = 10.6 LVPDD279 pKa = 4.32 LSCIGKK285 pKa = 10.29 LSTKK289 pKa = 10.2 EE290 pKa = 3.51 EE291 pKa = 4.03 AAGKK295 pKa = 9.74 VRR297 pKa = 11.84 VFAMVDD303 pKa = 2.32 IWTQSILNPLHH314 pKa = 6.71 KK315 pKa = 10.3 KK316 pKa = 8.81 IFSIIRR322 pKa = 11.84 EE323 pKa = 4.43 LPTDD327 pKa = 3.6 GTFDD331 pKa = 3.33 QLKK334 pKa = 10.04 PLDD337 pKa = 4.18 RR338 pKa = 11.84 LHH340 pKa = 6.86 EE341 pKa = 4.63 LSTQDD346 pKa = 3.61 RR347 pKa = 11.84 FSFDD351 pKa = 3.18 LSAATDD357 pKa = 3.93 RR358 pKa = 11.84 LPLTLQKK365 pKa = 10.86 DD366 pKa = 3.44 ILTLLVSPSFAEE378 pKa = 3.88 AWGTALVGRR387 pKa = 11.84 PYY389 pKa = 10.38 KK390 pKa = 10.01 WKK392 pKa = 10.49 FGTTEE397 pKa = 4.12 DD398 pKa = 3.49 EE399 pKa = 4.12 LMYY402 pKa = 10.94 SVGQPMGALSSWGMLALTHH421 pKa = 5.49 HH422 pKa = 7.14 TIVQVAASRR431 pKa = 11.84 AGYY434 pKa = 10.29 KK435 pKa = 10.44 DD436 pKa = 4.38 LFLDD440 pKa = 3.91 YY441 pKa = 11.17 ALLGDD446 pKa = 5.67 DD447 pKa = 3.56 ICIANKK453 pKa = 10.14 AVADD457 pKa = 3.94 NYY459 pKa = 10.88 LLIMRR464 pKa = 11.84 DD465 pKa = 3.3 LGVEE469 pKa = 3.92 INLSKK474 pKa = 11.05 SLISSTGVVEE484 pKa = 4.74 FAKK487 pKa = 10.13 RR488 pKa = 11.84 WRR490 pKa = 11.84 VGQTDD495 pKa = 3.66 VSPASPALITRR506 pKa = 11.84 LLSNMNYY513 pKa = 10.27 LPVLILDD520 pKa = 4.13 LVNRR524 pKa = 11.84 GVKK527 pKa = 9.54 TIQNSEE533 pKa = 4.18 KK534 pKa = 10.53 LLSLSDD540 pKa = 3.79 SLHH543 pKa = 6.42 KK544 pKa = 10.14 IKK546 pKa = 10.79 KK547 pKa = 9.98 SITFSLIPYY556 pKa = 8.96 YY557 pKa = 10.63 DD558 pKa = 3.74 SEE560 pKa = 4.35 FAKK563 pKa = 10.79 CLLPLKK569 pKa = 10.65 GKK571 pKa = 10.21 DD572 pKa = 3.05 KK573 pKa = 11.26 SFSQKK578 pKa = 10.14 EE579 pKa = 3.86 LFALYY584 pKa = 10.42 VHH586 pKa = 6.66 TDD588 pKa = 3.85 RR589 pKa = 11.84 IFNKK593 pKa = 10.27 LGLLEE598 pKa = 3.8 FHH600 pKa = 7.02 KK601 pKa = 11.06 AKK603 pKa = 10.99 DD604 pKa = 3.59 GDD606 pKa = 3.92 QEE608 pKa = 4.12 NSKK611 pKa = 10.69 LSQSYY616 pKa = 10.25 LYY618 pKa = 10.77 NILDD622 pKa = 3.72 NSKK625 pKa = 10.83 LPLPAITSQIDD636 pKa = 3.57 NILIAQMKK644 pKa = 8.92 TITNVPTRR652 pKa = 11.84 NWLNNRR658 pKa = 11.84 DD659 pKa = 3.55 INLLTFEE666 pKa = 4.86 CWHH669 pKa = 6.87 SYY671 pKa = 10.9 LIDD674 pKa = 3.76 TLSEE678 pKa = 3.88 LSSISKK684 pKa = 9.02 VVPDD688 pKa = 3.14 YY689 pKa = 11.18 TIIKK693 pKa = 10.41 DD694 pKa = 3.67 NEE696 pKa = 4.19 KK697 pKa = 10.41 PDD699 pKa = 3.83 NLEE702 pKa = 3.45 MRR704 pKa = 11.84 RR705 pKa = 11.84 NLLFMQEE712 pKa = 4.53 LIKK715 pKa = 10.59 DD716 pKa = 3.99 LKK718 pKa = 10.13 KK719 pKa = 9.04 WNPGIFEE726 pKa = 5.45 IIPEE730 pKa = 4.53 SILKK734 pKa = 10.26 EE735 pKa = 4.12 EE736 pKa = 4.37 EE737 pKa = 4.03 VV738 pKa = 3.56
Molecular weight: 84.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.706
IPC2_protein 8.639
IPC_protein 8.507
Toseland 9.282
ProMoST 9.048
Dawson 9.56
Bjellqvist 9.282
Wikipedia 9.721
Rodwell 9.853
Grimsley 9.633
Solomon 9.589
Lehninger 9.545
Nozaki 9.385
DTASelect 9.238
Thurlkill 9.414
EMBOSS 9.721
Sillero 9.502
Patrickios 4.94
IPC_peptide 9.589
IPC2_peptide 7.79
IPC2.peptide.svr19 7.725
Protein with the highest isoelectric point:
>tr|A5YRF7|A5YRF7_9VIRU RNA-dependent RNA polymerase OS=Botrytis cinerea mitovirus 1 OX=444193 GN=RDRP PE=4 SV=3
MM1 pKa = 7.27 KK2 pKa = 10.2 HH3 pKa = 6.41 LFRR6 pKa = 11.84 IKK8 pKa = 10.14 RR9 pKa = 11.84 KK10 pKa = 9.69 AVIKK14 pKa = 10.78 NSTTRR19 pKa = 11.84 LPDD22 pKa = 3.96 HH23 pKa = 6.8 LWLGYY28 pKa = 10.63 VKK30 pKa = 10.7 VLVWLYY36 pKa = 9.65 DD37 pKa = 3.18 IPSAHH42 pKa = 5.68 RR43 pKa = 11.84 TYY45 pKa = 10.8 FFEE48 pKa = 4.47 LLNRR52 pKa = 11.84 IQSLRR57 pKa = 11.84 RR58 pKa = 11.84 NSGDD62 pKa = 2.81 TWTVGYY68 pKa = 10.28 LKK70 pKa = 10.26 EE71 pKa = 4.06 SHH73 pKa = 6.64 RR74 pKa = 11.84 LSSHH78 pKa = 5.36 WLSGNPDD85 pKa = 3.21 VCSGSIRR92 pKa = 11.84 VSVRR96 pKa = 11.84 GLPLIIPGRR105 pKa = 11.84 LRR107 pKa = 11.84 NVIYY111 pKa = 10.55 SRR113 pKa = 11.84 NNINITRR120 pKa = 11.84 ALLTLLSTFRR130 pKa = 11.84 VMSATPKK137 pKa = 10.49 LKK139 pKa = 10.63 LSTITDD145 pKa = 3.73 PFNGVNEE152 pKa = 4.34 TLPSLEE158 pKa = 3.91 VSRR161 pKa = 11.84 ALKK164 pKa = 10.74 NILQGNKK171 pKa = 7.26 FHH173 pKa = 7.32 FKK175 pKa = 10.76 KK176 pKa = 10.62 EE177 pKa = 4.33 DD178 pKa = 3.09 IKK180 pKa = 11.45 DD181 pKa = 3.69 PYY183 pKa = 10.6 SVNRR187 pKa = 11.84 LMDD190 pKa = 3.2 ITKK193 pKa = 10.1 ADD195 pKa = 3.87 PNGGVSLRR203 pKa = 11.84 NVIIDD208 pKa = 3.97 AYY210 pKa = 10.95 ALMKK214 pKa = 10.0 HH215 pKa = 5.83 PKK217 pKa = 9.69 VYY219 pKa = 10.89 ASLLEE224 pKa = 4.2 LSNVMSPYY232 pKa = 10.47 LGSLLRR238 pKa = 11.84 NQTTIIRR245 pKa = 11.84 AIIDD249 pKa = 3.51 YY250 pKa = 10.87 DD251 pKa = 4.11 PIEE254 pKa = 4.1 WEE256 pKa = 4.05 RR257 pKa = 11.84 IKK259 pKa = 11.07 PFMAPIYY266 pKa = 10.46 QNIKK270 pKa = 10.69 DD271 pKa = 3.84 RR272 pKa = 11.84 TLKK275 pKa = 10.6 LVPDD279 pKa = 4.32 LSCIGKK285 pKa = 10.29 LSTKK289 pKa = 10.2 EE290 pKa = 3.51 EE291 pKa = 4.03 AAGKK295 pKa = 9.74 VRR297 pKa = 11.84 VFAMVDD303 pKa = 2.32 IWTQSILNPLHH314 pKa = 6.71 KK315 pKa = 10.3 KK316 pKa = 8.81 IFSIIRR322 pKa = 11.84 EE323 pKa = 4.43 LPTDD327 pKa = 3.6 GTFDD331 pKa = 3.33 QLKK334 pKa = 10.04 PLDD337 pKa = 4.18 RR338 pKa = 11.84 LHH340 pKa = 6.86 EE341 pKa = 4.63 LSTQDD346 pKa = 3.61 RR347 pKa = 11.84 FSFDD351 pKa = 3.18 LSAATDD357 pKa = 3.93 RR358 pKa = 11.84 LPLTLQKK365 pKa = 10.86 DD366 pKa = 3.44 ILTLLVSPSFAEE378 pKa = 3.88 AWGTALVGRR387 pKa = 11.84 PYY389 pKa = 10.38 KK390 pKa = 10.01 WKK392 pKa = 10.49 FGTTEE397 pKa = 4.12 DD398 pKa = 3.49 EE399 pKa = 4.12 LMYY402 pKa = 10.94 SVGQPMGALSSWGMLALTHH421 pKa = 5.49 HH422 pKa = 7.14 TIVQVAASRR431 pKa = 11.84 AGYY434 pKa = 10.29 KK435 pKa = 10.44 DD436 pKa = 4.38 LFLDD440 pKa = 3.91 YY441 pKa = 11.17 ALLGDD446 pKa = 5.67 DD447 pKa = 3.56 ICIANKK453 pKa = 10.14 AVADD457 pKa = 3.94 NYY459 pKa = 10.88 LLIMRR464 pKa = 11.84 DD465 pKa = 3.3 LGVEE469 pKa = 3.92 INLSKK474 pKa = 11.05 SLISSTGVVEE484 pKa = 4.74 FAKK487 pKa = 10.13 RR488 pKa = 11.84 WRR490 pKa = 11.84 VGQTDD495 pKa = 3.66 VSPASPALITRR506 pKa = 11.84 LLSNMNYY513 pKa = 10.27 LPVLILDD520 pKa = 4.13 LVNRR524 pKa = 11.84 GVKK527 pKa = 9.54 TIQNSEE533 pKa = 4.18 KK534 pKa = 10.53 LLSLSDD540 pKa = 3.79 SLHH543 pKa = 6.42 KK544 pKa = 10.14 IKK546 pKa = 10.79 KK547 pKa = 9.98 SITFSLIPYY556 pKa = 8.96 YY557 pKa = 10.63 DD558 pKa = 3.74 SEE560 pKa = 4.35 FAKK563 pKa = 10.79 CLLPLKK569 pKa = 10.65 GKK571 pKa = 10.21 DD572 pKa = 3.05 KK573 pKa = 11.26 SFSQKK578 pKa = 10.14 EE579 pKa = 3.86 LFALYY584 pKa = 10.42 VHH586 pKa = 6.66 TDD588 pKa = 3.85 RR589 pKa = 11.84 IFNKK593 pKa = 10.27 LGLLEE598 pKa = 3.8 FHH600 pKa = 7.02 KK601 pKa = 11.06 AKK603 pKa = 10.99 DD604 pKa = 3.59 GDD606 pKa = 3.92 QEE608 pKa = 4.12 NSKK611 pKa = 10.69 LSQSYY616 pKa = 10.25 LYY618 pKa = 10.77 NILDD622 pKa = 3.72 NSKK625 pKa = 10.83 LPLPAITSQIDD636 pKa = 3.57 NILIAQMKK644 pKa = 8.92 TITNVPTRR652 pKa = 11.84 NWLNNRR658 pKa = 11.84 DD659 pKa = 3.55 INLLTFEE666 pKa = 4.86 CWHH669 pKa = 6.87 SYY671 pKa = 10.9 LIDD674 pKa = 3.76 TLSEE678 pKa = 3.88 LSSISKK684 pKa = 9.02 VVPDD688 pKa = 3.14 YY689 pKa = 11.18 TIIKK693 pKa = 10.41 DD694 pKa = 3.67 NEE696 pKa = 4.19 KK697 pKa = 10.41 PDD699 pKa = 3.83 NLEE702 pKa = 3.45 MRR704 pKa = 11.84 RR705 pKa = 11.84 NLLFMQEE712 pKa = 4.53 LIKK715 pKa = 10.59 DD716 pKa = 3.99 LKK718 pKa = 10.13 KK719 pKa = 9.04 WNPGIFEE726 pKa = 5.45 IIPEE730 pKa = 4.53 SILKK734 pKa = 10.26 EE735 pKa = 4.12 EE736 pKa = 4.37 EE737 pKa = 4.03 VV738 pKa = 3.56
Molecular weight: 84.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.706
IPC2_protein 8.639
IPC_protein 8.507
Toseland 9.282
ProMoST 9.048
Dawson 9.56
Bjellqvist 9.282
Wikipedia 9.721
Rodwell 9.853
Grimsley 9.633
Solomon 9.589
Lehninger 9.545
Nozaki 9.385
DTASelect 9.238
Thurlkill 9.414
EMBOSS 9.721
Sillero 9.502
Patrickios 4.94
IPC_peptide 9.589
IPC2_peptide 7.79
IPC2.peptide.svr19 7.725
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
738
738
738
738.0
84.03
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.878 ± 0.0
0.678 ± 0.0
6.098 ± 0.0
4.336 ± 0.0
3.523 ± 0.0
4.201 ± 0.0
2.033 ± 0.0
8.13 ± 0.0
7.453 ± 0.0
14.092 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.033 ± 0.0
5.42 ± 0.0
4.743 ± 0.0
2.439 ± 0.0
5.285 ± 0.0
8.537 ± 0.0
5.691 ± 0.0
5.42 ± 0.0
1.762 ± 0.0
3.252 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here