Penicillium aurantiogriseum totivirus 1
Average proteome isoelectric point is 6.68
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0S2GML3|A0A0S2GML3_9VIRU RNA-directed RNA polymerase OS=Penicillium aurantiogriseum totivirus 1 OX=1755467 GN=RdRp PE=3 SV=1
MM1 pKa = 7.83 ASGQGFPQQATSPLTGTIADD21 pKa = 3.82 PVGGVIADD29 pKa = 3.63 SNSYY33 pKa = 9.44 RR34 pKa = 11.84 RR35 pKa = 11.84 YY36 pKa = 8.77 RR37 pKa = 11.84 AGVYY41 pKa = 8.22 MQLPDD46 pKa = 3.65 HH47 pKa = 6.83 GSTTSHH53 pKa = 6.21 ARR55 pKa = 11.84 SIFYY59 pKa = 10.34 EE60 pKa = 3.77 VGRR63 pKa = 11.84 RR64 pKa = 11.84 HH65 pKa = 6.43 SGAKK69 pKa = 9.63 PLFARR74 pKa = 11.84 PPEE77 pKa = 3.95 SSIPIDD83 pKa = 3.5 CSVDD87 pKa = 3.18 INSAEE92 pKa = 3.76 ASNFEE97 pKa = 3.72 GTARR101 pKa = 11.84 RR102 pKa = 11.84 FSNFSPQWVKK112 pKa = 10.55 MDD114 pKa = 4.42 LAAIAEE120 pKa = 4.17 RR121 pKa = 11.84 LAKK124 pKa = 10.26 GVAGYY129 pKa = 10.3 SLYY132 pKa = 11.13 GAVDD136 pKa = 3.59 TTTMRR141 pKa = 11.84 GGQPIRR147 pKa = 11.84 VVALGTLDD155 pKa = 3.82 SPQTASTSSVFIPRR169 pKa = 11.84 TVDD172 pKa = 3.11 TVGNDD177 pKa = 2.9 HH178 pKa = 6.29 VFAVLVSAANGEE190 pKa = 4.5 GASVTTDD197 pKa = 3.28 VVRR200 pKa = 11.84 LDD202 pKa = 4.16 ANTNQPIIPAVAGGAFPSACVEE224 pKa = 3.94 ALRR227 pKa = 11.84 VLGANMEE234 pKa = 4.16 ASGAGDD240 pKa = 3.48 IFAYY244 pKa = 10.29 AVTRR248 pKa = 11.84 GIHH251 pKa = 5.59 AAVSVVGHH259 pKa = 5.54 TDD261 pKa = 2.53 EE262 pKa = 5.24 GGLMRR267 pKa = 11.84 GLLRR271 pKa = 11.84 HH272 pKa = 6.27 DD273 pKa = 4.29 RR274 pKa = 11.84 FRR276 pKa = 11.84 VPYY279 pKa = 10.46 GGINQALRR287 pKa = 11.84 DD288 pKa = 4.16 YY289 pKa = 10.85 PSLPALASTSPSAIAAWVDD308 pKa = 4.31 AIALKK313 pKa = 9.12 TAAAVGHH320 pKa = 6.94 CDD322 pKa = 3.33 PLVPGEE328 pKa = 4.55 GGWFPSVFSARR339 pKa = 11.84 EE340 pKa = 3.88 GALLPAGSAGEE351 pKa = 4.07 EE352 pKa = 4.21 ATDD355 pKa = 3.31 QDD357 pKa = 3.04 ARR359 pKa = 11.84 AIGRR363 pKa = 11.84 QIAADD368 pKa = 4.32 LGRR371 pKa = 11.84 FAPLYY376 pKa = 9.45 TRR378 pKa = 11.84 ALTRR382 pKa = 11.84 IFGLHH387 pKa = 5.58 TSSGVAEE394 pKa = 4.23 AHH396 pKa = 6.37 FSTAGSRR403 pKa = 11.84 ALNQEE408 pKa = 4.2 TAVDD412 pKa = 3.39 RR413 pKa = 11.84 HH414 pKa = 6.13 LKK416 pKa = 9.93 FKK418 pKa = 10.17 TVAPYY423 pKa = 9.8 FWIEE427 pKa = 3.83 PTSLLEE433 pKa = 3.99 STAFGSLAEE442 pKa = 4.19 SEE444 pKa = 4.96 GYY446 pKa = 8.46 GAKK449 pKa = 8.63 VTPGDD454 pKa = 3.84 TATVPCFEE462 pKa = 4.99 RR463 pKa = 11.84 FRR465 pKa = 11.84 SLAKK469 pKa = 10.65 GLTATHH475 pKa = 5.88 ATIAFKK481 pKa = 10.39 MRR483 pKa = 11.84 TARR486 pKa = 11.84 TSGLVSALAANPAALADD503 pKa = 3.37 IKK505 pKa = 11.01 LFQFDD510 pKa = 3.99 TASVALPGDD519 pKa = 3.49 QGPTNGTVSEE529 pKa = 4.19 KK530 pKa = 10.66 HH531 pKa = 4.98 QAADD535 pKa = 3.81 PLSSYY540 pKa = 10.7 LWTRR544 pKa = 11.84 GQSCFPAPAEE554 pKa = 4.2 FININSNYY562 pKa = 8.4 GGKK565 pKa = 10.05 VKK567 pKa = 10.65 LVDD570 pKa = 3.13 WDD572 pKa = 3.49 QDD574 pKa = 3.76 FNATLSDD581 pKa = 4.0 LPNDD585 pKa = 3.65 SEE587 pKa = 4.38 MASDD591 pKa = 5.4 LITWRR596 pKa = 11.84 VSVPAGLPTGPSNASDD612 pKa = 3.6 RR613 pKa = 11.84 NAKK616 pKa = 9.66 RR617 pKa = 11.84 ARR619 pKa = 11.84 SRR621 pKa = 11.84 AASALAQAVARR632 pKa = 11.84 SRR634 pKa = 11.84 SFGLAISPTMEE645 pKa = 3.93 VSEE648 pKa = 4.57 VPPSFDD654 pKa = 3.26 EE655 pKa = 4.05 PSRR658 pKa = 11.84 PFKK661 pKa = 10.36 DD662 pKa = 3.54 TEE664 pKa = 4.14 PTGLGIDD671 pKa = 3.87 PGVTTTKK678 pKa = 10.62 AEE680 pKa = 3.97 PSGIDD685 pKa = 2.66 TGRR688 pKa = 11.84 TVRR691 pKa = 11.84 GAALNPIAHH700 pKa = 7.06 HH701 pKa = 6.23 EE702 pKa = 4.14 PQRR705 pKa = 11.84 APQMPQRR712 pKa = 11.84 GAGFAAGPPPAPQGGAPPPPPGAGGAPPPPPSQGPPINPDD752 pKa = 3.84 PLPTPPTSLPDD763 pKa = 3.46 AAANAPAAPAQQ774 pKa = 3.59
Molecular weight: 80.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.914
IPC2_protein 5.779
IPC_protein 5.855
Toseland 6.3
ProMoST 6.198
Dawson 6.135
Bjellqvist 6.135
Wikipedia 6.148
Rodwell 6.109
Grimsley 6.478
Solomon 6.135
Lehninger 6.122
Nozaki 6.402
DTASelect 6.59
Thurlkill 6.605
EMBOSS 6.576
Sillero 6.504
Patrickios 4.431
IPC_peptide 6.148
IPC2_peptide 6.491
IPC2.peptide.svr19 6.422
Protein with the highest isoelectric point:
>tr|A0A0S2GML3|A0A0S2GML3_9VIRU RNA-directed RNA polymerase OS=Penicillium aurantiogriseum totivirus 1 OX=1755467 GN=RdRp PE=3 SV=1
MM1 pKa = 7.29 IGSRR5 pKa = 11.84 VDD7 pKa = 3.38 DD8 pKa = 3.76 RR9 pKa = 11.84 TNAAGPIGLFLRR21 pKa = 11.84 GLLDD25 pKa = 3.71 PTWIDD30 pKa = 3.67 QIIKK34 pKa = 10.36 LPYY37 pKa = 9.2 AEE39 pKa = 4.97 QISEE43 pKa = 4.26 VYY45 pKa = 10.51 KK46 pKa = 10.98 PMFNGQRR53 pKa = 11.84 VSEE56 pKa = 4.27 LQRR59 pKa = 11.84 TAAAFLVPDD68 pKa = 4.99 FPVQTLLSEE77 pKa = 4.37 GGIMTLVSNVIPDD90 pKa = 3.92 PPTLGSSQAVRR101 pKa = 11.84 WANSIAQTVKK111 pKa = 10.89 LFPLKK116 pKa = 10.53 KK117 pKa = 10.47 NPAAANKK124 pKa = 9.49 VNVYY128 pKa = 10.54 LNEE131 pKa = 3.75 ILGDD135 pKa = 4.11 LKK137 pKa = 10.94 RR138 pKa = 11.84 EE139 pKa = 4.03 SPMQFIAAASALYY152 pKa = 10.19 HH153 pKa = 5.69 FRR155 pKa = 11.84 NRR157 pKa = 11.84 LFNDD161 pKa = 2.92 QAGAVVLYY169 pKa = 10.22 GAGLISSGVPDD180 pKa = 4.81 AFWVAANLVADD191 pKa = 4.26 PALAKK196 pKa = 10.76 AFTTVLKK203 pKa = 10.82 AVGANGSRR211 pKa = 11.84 LGAMLVEE218 pKa = 4.6 ANTLRR223 pKa = 11.84 GRR225 pKa = 11.84 DD226 pKa = 3.71 VDD228 pKa = 3.94 PCDD231 pKa = 3.98 LKK233 pKa = 11.57 AEE235 pKa = 4.21 ALYY238 pKa = 8.69 RR239 pKa = 11.84 TTTAVNDD246 pKa = 3.8 KK247 pKa = 10.67 LAVFPEE253 pKa = 3.9 EE254 pKa = 5.31 DD255 pKa = 3.14 LRR257 pKa = 11.84 QACRR261 pKa = 11.84 AIFSEE266 pKa = 4.96 EE267 pKa = 3.89 IAHH270 pKa = 6.08 GPEE273 pKa = 4.16 GLLVQYY279 pKa = 7.53 PTLEE283 pKa = 4.28 DD284 pKa = 3.17 HH285 pKa = 7.11 WDD287 pKa = 3.84 SRR289 pKa = 11.84 WGWAVNGAHH298 pKa = 6.54 SGHH301 pKa = 5.91 VSKK304 pKa = 10.72 EE305 pKa = 3.7 YY306 pKa = 11.08 ARR308 pKa = 11.84 APKK311 pKa = 8.77 PAGMLRR317 pKa = 11.84 EE318 pKa = 4.09 HH319 pKa = 6.28 RR320 pKa = 11.84 RR321 pKa = 11.84 AWLEE325 pKa = 4.02 SVSDD329 pKa = 4.11 DD330 pKa = 5.36 PRR332 pKa = 11.84 PTWDD336 pKa = 2.53 GHH338 pKa = 5.35 TYY340 pKa = 10.77 VSVSPKK346 pKa = 10.44 LEE348 pKa = 4.11 SGKK351 pKa = 8.28 TRR353 pKa = 11.84 AIFACDD359 pKa = 3.19 TVSYY363 pKa = 10.61 LAFEE367 pKa = 4.63 HH368 pKa = 6.77 LLAPVEE374 pKa = 4.12 RR375 pKa = 11.84 RR376 pKa = 11.84 WRR378 pKa = 11.84 HH379 pKa = 4.66 KK380 pKa = 10.95 NIILDD385 pKa = 3.89 PGKK388 pKa = 10.29 GGHH391 pKa = 6.24 VGMTFKK397 pKa = 10.45 TRR399 pKa = 11.84 AARR402 pKa = 11.84 DD403 pKa = 3.39 RR404 pKa = 11.84 AGVSMMLDD412 pKa = 3.13 YY413 pKa = 11.56 DD414 pKa = 4.41 DD415 pKa = 6.4 FNSQHH420 pKa = 6.56 SNRR423 pKa = 11.84 SMQILFEE430 pKa = 4.28 EE431 pKa = 4.94 LCDD434 pKa = 3.29 ITGYY438 pKa = 9.92 PSHH441 pKa = 7.06 LKK443 pKa = 9.19 DD444 pKa = 3.35 TLINSFDD451 pKa = 3.26 KK452 pKa = 11.14 CDD454 pKa = 3.45 IYY456 pKa = 11.31 LGAQRR461 pKa = 11.84 IGRR464 pKa = 11.84 ALGTLMSGHH473 pKa = 7.18 RR474 pKa = 11.84 GTTFINSVLNRR485 pKa = 11.84 VYY487 pKa = 10.84 LVLVLGADD495 pKa = 3.27 IVDD498 pKa = 4.49 DD499 pKa = 3.95 STTLHH504 pKa = 6.34 VGDD507 pKa = 4.49 DD508 pKa = 3.62 VYY510 pKa = 11.06 MGVRR514 pKa = 11.84 TYY516 pKa = 10.95 QRR518 pKa = 11.84 AGLICQKK525 pKa = 10.34 LASSCLRR532 pKa = 11.84 MNPIKK537 pKa = 10.5 QSVGHH542 pKa = 5.46 TTTEE546 pKa = 3.94 FLRR549 pKa = 11.84 NATSGRR555 pKa = 11.84 DD556 pKa = 3.33 TRR558 pKa = 11.84 GYY560 pKa = 9.55 FARR563 pKa = 11.84 GVAGIIAGNWANEE576 pKa = 4.36 TKK578 pKa = 10.7 LSPSEE583 pKa = 4.48 ALTSMLASCRR593 pKa = 11.84 TLINRR598 pKa = 11.84 SGVEE602 pKa = 4.06 RR603 pKa = 11.84 LPLLLFTGLVRR614 pKa = 11.84 LTGLPRR620 pKa = 11.84 EE621 pKa = 4.21 DD622 pKa = 3.41 HH623 pKa = 6.64 KK624 pKa = 11.44 KK625 pKa = 10.65 LRR627 pKa = 11.84 DD628 pKa = 3.43 LMLGVTALDD637 pKa = 3.7 NGPLFHH643 pKa = 7.23 HH644 pKa = 6.82 GGYY647 pKa = 9.07 YY648 pKa = 9.93 RR649 pKa = 11.84 SVRR652 pKa = 11.84 LHH654 pKa = 6.1 TGVTASDD661 pKa = 3.29 QHH663 pKa = 7.56 GYY665 pKa = 8.01 TPLPNSATASFLSRR679 pKa = 11.84 AAQPLEE685 pKa = 4.17 VTVLTQAGVSLVDD698 pKa = 3.4 TMQEE702 pKa = 3.62 ASFKK706 pKa = 10.55 KK707 pKa = 10.26 SLPARR712 pKa = 11.84 YY713 pKa = 8.96 RR714 pKa = 11.84 SYY716 pKa = 9.98 EE717 pKa = 4.09 TVRR720 pKa = 11.84 LGALCLTTAVGSADD734 pKa = 3.4 VADD737 pKa = 4.87 LVNTPPPRR745 pKa = 11.84 GVLQKK750 pKa = 11.16 YY751 pKa = 9.44 PLLTLARR758 pKa = 11.84 NRR760 pKa = 11.84 LDD762 pKa = 3.27 EE763 pKa = 4.34 SLVRR767 pKa = 11.84 WAVKK771 pKa = 9.71 QAGGNPNAVDD781 pKa = 4.62 LEE783 pKa = 4.26 MEE785 pKa = 3.67 AWGEE789 pKa = 4.06 YY790 pKa = 9.51 KK791 pKa = 10.24 HH792 pKa = 6.41 GCIIASALSYY802 pKa = 11.1 SDD804 pKa = 2.92 AAMYY808 pKa = 8.67 GHH810 pKa = 6.54 RR811 pKa = 11.84 TVSSVLTCPINVYY824 pKa = 8.96 VV825 pKa = 3.98
Molecular weight: 90.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.442
IPC2_protein 7.79
IPC_protein 7.644
Toseland 7.41
ProMoST 8.2
Dawson 8.346
Bjellqvist 8.668
Wikipedia 8.287
Rodwell 8.375
Grimsley 7.483
Solomon 8.419
Lehninger 8.419
Nozaki 8.785
DTASelect 8.434
Thurlkill 8.478
EMBOSS 8.551
Sillero 8.77
Patrickios 4.406
IPC_peptide 8.404
IPC2_peptide 7.644
IPC2.peptide.svr19 7.735
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1599
774
825
799.5
85.27
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.57 ± 1.619
1.001 ± 0.225
5.378 ± 0.134
3.94 ± 0.123
3.502 ± 0.32
8.568 ± 0.549
2.314 ± 0.239
3.877 ± 0.001
2.877 ± 0.515
9.006 ± 1.537
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.751 ± 0.292
3.502 ± 0.337
7.192 ± 1.506
2.939 ± 0.103
6.442 ± 0.317
7.692 ± 0.531
6.754 ± 0.224
6.942 ± 0.471
1.188 ± 0.18
2.564 ± 0.48
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here