Amino acid dipepetide frequency for Streptococcus phage IPP19

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.95AlaAla: 3.95 ± 1.38
0.47AlaCys: 0.47 ± 0.24
5.83AlaAsp: 5.83 ± 0.77
5.548AlaGlu: 5.548 ± 0.76
2.633AlaPhe: 2.633 ± 0.36
3.95AlaGly: 3.95 ± 0.889
0.376AlaHis: 0.376 ± 0.155
5.36AlaIle: 5.36 ± 1.302
3.856AlaLys: 3.856 ± 0.633
8.181AlaLeu: 8.181 ± 1.017
1.975AlaMet: 1.975 ± 0.328
4.232AlaAsn: 4.232 ± 0.916
1.222AlaPro: 1.222 ± 0.318
3.479AlaGln: 3.479 ± 0.987
2.821AlaArg: 2.821 ± 0.529
3.762AlaSer: 3.762 ± 0.513
4.232AlaThr: 4.232 ± 1.053
4.514AlaVal: 4.514 ± 0.613
1.222AlaTrp: 1.222 ± 0.477
1.975AlaTyr: 1.975 ± 0.49
0.0AlaXaa: 0.0 ± 0.0
Cys
0.094CysAla: 0.094 ± 0.105
0.0CysCys: 0.0 ± 0.0
0.188CysAsp: 0.188 ± 0.138
0.658CysGlu: 0.658 ± 0.295
0.376CysPhe: 0.376 ± 0.232
0.282CysGly: 0.282 ± 0.143
0.094CysHis: 0.094 ± 0.099
0.376CysIle: 0.376 ± 0.221
0.376CysLys: 0.376 ± 0.226
0.658CysLeu: 0.658 ± 0.301
0.0CysMet: 0.0 ± 0.0
0.0CysAsn: 0.0 ± 0.0
0.188CysPro: 0.188 ± 0.125
0.188CysGln: 0.188 ± 0.133
0.47CysArg: 0.47 ± 0.228
0.282CysSer: 0.282 ± 0.205
0.282CysThr: 0.282 ± 0.189
0.282CysVal: 0.282 ± 0.142
0.0CysTrp: 0.0 ± 0.0
0.658CysTyr: 0.658 ± 0.22
0.0CysXaa: 0.0 ± 0.0
Asp
4.044AspAla: 4.044 ± 0.485
0.282AspCys: 0.282 ± 0.167
3.385AspAsp: 3.385 ± 0.766
4.608AspGlu: 4.608 ± 1.4
2.351AspPhe: 2.351 ± 0.642
4.608AspGly: 4.608 ± 0.687
0.94AspHis: 0.94 ± 0.317
5.266AspIle: 5.266 ± 0.729
4.89AspLys: 4.89 ± 0.482
5.078AspLeu: 5.078 ± 0.75
1.317AspMet: 1.317 ± 0.418
2.445AspAsn: 2.445 ± 0.536
1.222AspPro: 1.222 ± 0.412
1.505AspGln: 1.505 ± 0.379
1.881AspArg: 1.881 ± 0.527
2.539AspSer: 2.539 ± 0.509
2.821AspThr: 2.821 ± 0.466
3.479AspVal: 3.479 ± 0.584
1.222AspTrp: 1.222 ± 0.355
3.385AspTyr: 3.385 ± 0.708
0.0AspXaa: 0.0 ± 0.0
Glu
6.301GluAla: 6.301 ± 1.494
0.47GluCys: 0.47 ± 0.236
3.573GluAsp: 3.573 ± 0.731
7.147GluGlu: 7.147 ± 1.221
3.95GluPhe: 3.95 ± 0.545
3.573GluGly: 3.573 ± 0.572
0.752GluHis: 0.752 ± 0.272
6.112GluIle: 6.112 ± 0.719
8.181GluLys: 8.181 ± 1.222
9.216GluLeu: 9.216 ± 1.041
3.009GluMet: 3.009 ± 0.7
5.454GluAsn: 5.454 ± 0.744
1.975GluPro: 1.975 ± 0.646
4.232GluGln: 4.232 ± 1.117
3.479GluArg: 3.479 ± 0.651
4.42GluSer: 4.42 ± 0.643
4.514GluThr: 4.514 ± 0.899
5.36GluVal: 5.36 ± 0.883
1.128GluTrp: 1.128 ± 0.327
2.351GluTyr: 2.351 ± 0.496
0.0GluXaa: 0.0 ± 0.0
Phe
2.163PheAla: 2.163 ± 0.503
0.188PheCys: 0.188 ± 0.141
3.667PheAsp: 3.667 ± 0.628
2.727PheGlu: 2.727 ± 0.541
1.881PhePhe: 1.881 ± 0.686
3.103PheGly: 3.103 ± 0.824
0.564PheHis: 0.564 ± 0.279
3.385PheIle: 3.385 ± 0.882
3.856PheLys: 3.856 ± 0.519
2.727PheLeu: 2.727 ± 0.583
1.317PheMet: 1.317 ± 0.42
3.667PheAsn: 3.667 ± 0.764
0.94PhePro: 0.94 ± 0.368
1.505PheGln: 1.505 ± 0.396
1.505PheArg: 1.505 ± 0.287
2.821PheSer: 2.821 ± 0.966
2.445PheThr: 2.445 ± 0.32
2.163PheVal: 2.163 ± 0.427
0.752PheTrp: 0.752 ± 0.279
1.599PheTyr: 1.599 ± 0.371
0.0PheXaa: 0.0 ± 0.0
Gly
4.232GlyAla: 4.232 ± 0.845
0.188GlyCys: 0.188 ± 0.165
3.573GlyAsp: 3.573 ± 0.642
3.385GlyGlu: 3.385 ± 0.45
2.633GlyPhe: 2.633 ± 0.554
3.856GlyGly: 3.856 ± 0.449
0.658GlyHis: 0.658 ± 0.224
4.514GlyIle: 4.514 ± 0.745
4.514GlyLys: 4.514 ± 0.412
4.984GlyLeu: 4.984 ± 0.7
1.787GlyMet: 1.787 ± 0.53
3.479GlyAsn: 3.479 ± 0.798
0.752GlyPro: 0.752 ± 0.219
2.821GlyGln: 2.821 ± 0.539
3.667GlyArg: 3.667 ± 0.838
4.044GlySer: 4.044 ± 0.765
3.479GlyThr: 3.479 ± 0.474
3.762GlyVal: 3.762 ± 0.424
1.505GlyTrp: 1.505 ± 0.614
2.821GlyTyr: 2.821 ± 0.574
0.0GlyXaa: 0.0 ± 0.0
His
0.564HisAla: 0.564 ± 0.213
0.188HisCys: 0.188 ± 0.141
0.658HisAsp: 0.658 ± 0.334
1.128HisGlu: 1.128 ± 0.34
1.128HisPhe: 1.128 ± 0.37
0.564HisGly: 0.564 ± 0.286
0.282HisHis: 0.282 ± 0.194
1.693HisIle: 1.693 ± 0.392
0.752HisLys: 0.752 ± 0.256
1.222HisLeu: 1.222 ± 0.372
0.376HisMet: 0.376 ± 0.164
0.846HisAsn: 0.846 ± 0.352
0.376HisPro: 0.376 ± 0.176
0.658HisGln: 0.658 ± 0.329
0.658HisArg: 0.658 ± 0.287
1.222HisSer: 1.222 ± 0.557
0.658HisThr: 0.658 ± 0.345
0.94HisVal: 0.94 ± 0.302
0.0HisTrp: 0.0 ± 0.0
0.564HisTyr: 0.564 ± 0.255
0.0HisXaa: 0.0 ± 0.0
Ile
5.266IleAla: 5.266 ± 0.533
0.282IleCys: 0.282 ± 0.199
3.197IleAsp: 3.197 ± 0.656
5.736IleGlu: 5.736 ± 0.755
2.351IlePhe: 2.351 ± 0.521
3.667IleGly: 3.667 ± 0.778
0.94IleHis: 0.94 ± 0.303
3.385IleIle: 3.385 ± 0.884
6.395IleLys: 6.395 ± 0.832
4.984IleLeu: 4.984 ± 0.846
1.411IleMet: 1.411 ± 0.361
3.385IleAsn: 3.385 ± 0.487
1.693IlePro: 1.693 ± 0.325
2.539IleGln: 2.539 ± 0.375
3.762IleArg: 3.762 ± 0.569
5.36IleSer: 5.36 ± 0.977
5.172IleThr: 5.172 ± 0.624
4.42IleVal: 4.42 ± 0.974
0.752IleTrp: 0.752 ± 0.267
1.599IleTyr: 1.599 ± 0.539
0.0IleXaa: 0.0 ± 0.0
Lys
5.83LysAla: 5.83 ± 1.155
0.376LysCys: 0.376 ± 0.173
5.924LysAsp: 5.924 ± 0.788
7.523LysGlu: 7.523 ± 1.215
2.821LysPhe: 2.821 ± 0.491
4.608LysGly: 4.608 ± 0.777
1.975LysHis: 1.975 ± 0.421
4.044LysIle: 4.044 ± 0.768
7.147LysLys: 7.147 ± 1.338
6.583LysLeu: 6.583 ± 0.657
2.163LysMet: 2.163 ± 0.408
4.42LysAsn: 4.42 ± 0.569
2.539LysPro: 2.539 ± 0.555
3.856LysGln: 3.856 ± 0.486
4.044LysArg: 4.044 ± 0.891
5.454LysSer: 5.454 ± 0.626
6.018LysThr: 6.018 ± 0.787
4.89LysVal: 4.89 ± 0.688
1.034LysTrp: 1.034 ± 0.328
2.821LysTyr: 2.821 ± 0.43
0.0LysXaa: 0.0 ± 0.0
Leu
6.489LeuAla: 6.489 ± 0.784
0.188LeuCys: 0.188 ± 0.134
5.172LeuAsp: 5.172 ± 0.608
8.369LeuGlu: 8.369 ± 0.829
3.479LeuPhe: 3.479 ± 0.597
6.395LeuGly: 6.395 ± 0.516
1.222LeuHis: 1.222 ± 0.357
4.702LeuIle: 4.702 ± 0.676
7.805LeuLys: 7.805 ± 0.977
5.924LeuLeu: 5.924 ± 1.249
1.505LeuMet: 1.505 ± 0.518
4.984LeuAsn: 4.984 ± 0.596
2.257LeuPro: 2.257 ± 0.494
3.103LeuGln: 3.103 ± 1.07
4.796LeuArg: 4.796 ± 0.815
6.395LeuSer: 6.395 ± 0.691
4.608LeuThr: 4.608 ± 0.436
3.762LeuVal: 3.762 ± 0.626
0.752LeuTrp: 0.752 ± 0.23
2.633LeuTyr: 2.633 ± 0.467
0.0LeuXaa: 0.0 ± 0.0
Met
1.881MetAla: 1.881 ± 0.57
0.188MetCys: 0.188 ± 0.144
1.222MetAsp: 1.222 ± 0.397
2.163MetGlu: 2.163 ± 0.575
0.846MetPhe: 0.846 ± 0.356
1.222MetGly: 1.222 ± 0.302
0.0MetHis: 0.0 ± 0.0
1.317MetIle: 1.317 ± 0.473
2.163MetLys: 2.163 ± 0.443
2.069MetLeu: 2.069 ± 0.614
0.47MetMet: 0.47 ± 0.22
1.693MetAsn: 1.693 ± 0.422
0.376MetPro: 0.376 ± 0.183
0.658MetGln: 0.658 ± 0.21
1.411MetArg: 1.411 ± 0.437
1.787MetSer: 1.787 ± 0.508
2.069MetThr: 2.069 ± 0.435
1.505MetVal: 1.505 ± 0.34
0.094MetTrp: 0.094 ± 0.083
0.47MetTyr: 0.47 ± 0.218
0.0MetXaa: 0.0 ± 0.0
Asn
3.479AsnAla: 3.479 ± 0.822
0.376AsnCys: 0.376 ± 0.237
2.445AsnAsp: 2.445 ± 0.425
5.078AsnGlu: 5.078 ± 0.602
2.915AsnPhe: 2.915 ± 0.661
3.95AsnGly: 3.95 ± 0.731
1.411AsnHis: 1.411 ± 0.365
3.762AsnIle: 3.762 ± 0.664
5.36AsnLys: 5.36 ± 0.648
4.796AsnLeu: 4.796 ± 0.738
0.752AsnMet: 0.752 ± 0.39
2.633AsnAsn: 2.633 ± 0.549
2.539AsnPro: 2.539 ± 0.567
3.762AsnGln: 3.762 ± 0.64
1.881AsnArg: 1.881 ± 0.375
3.479AsnSer: 3.479 ± 0.759
2.821AsnThr: 2.821 ± 0.409
4.232AsnVal: 4.232 ± 0.634
0.564AsnTrp: 0.564 ± 0.222
0.658AsnTyr: 0.658 ± 0.211
0.0AsnXaa: 0.0 ± 0.0
Pro
1.881ProAla: 1.881 ± 0.418
0.282ProCys: 0.282 ± 0.193
2.163ProAsp: 2.163 ± 0.634
2.163ProGlu: 2.163 ± 0.59
0.94ProPhe: 0.94 ± 0.369
0.846ProGly: 0.846 ± 0.358
0.658ProHis: 0.658 ± 0.217
1.693ProIle: 1.693 ± 0.45
2.351ProLys: 2.351 ± 0.501
2.163ProLeu: 2.163 ± 0.492
0.188ProMet: 0.188 ± 0.15
0.752ProAsn: 0.752 ± 0.324
0.282ProPro: 0.282 ± 0.261
1.317ProGln: 1.317 ± 0.478
0.94ProArg: 0.94 ± 0.294
1.505ProSer: 1.505 ± 0.478
1.787ProThr: 1.787 ± 0.582
2.069ProVal: 2.069 ± 0.458
0.282ProTrp: 0.282 ± 0.187
0.752ProTyr: 0.752 ± 0.373
0.0ProXaa: 0.0 ± 0.0
Gln
4.796GlnAla: 4.796 ± 1.187
0.094GlnCys: 0.094 ± 0.109
1.034GlnAsp: 1.034 ± 0.302
4.89GlnGlu: 4.89 ± 0.807
1.505GlnPhe: 1.505 ± 0.283
2.539GlnGly: 2.539 ± 0.472
0.376GlnHis: 0.376 ± 0.244
1.787GlnIle: 1.787 ± 0.454
3.573GlnLys: 3.573 ± 0.591
3.197GlnLeu: 3.197 ± 0.707
1.411GlnMet: 1.411 ± 0.419
2.633GlnAsn: 2.633 ± 0.505
1.411GlnPro: 1.411 ± 0.368
1.222GlnGln: 1.222 ± 0.432
2.163GlnArg: 2.163 ± 0.6
3.197GlnSer: 3.197 ± 0.551
3.009GlnThr: 3.009 ± 0.444
2.539GlnVal: 2.539 ± 0.574
0.376GlnTrp: 0.376 ± 0.215
1.034GlnTyr: 1.034 ± 0.405
0.0GlnXaa: 0.0 ± 0.0
Arg
3.197ArgAla: 3.197 ± 0.55
0.282ArgCys: 0.282 ± 0.146
2.163ArgAsp: 2.163 ± 0.477
3.95ArgGlu: 3.95 ± 0.624
1.975ArgPhe: 1.975 ± 0.538
1.599ArgGly: 1.599 ± 0.51
0.564ArgHis: 0.564 ± 0.286
3.197ArgIle: 3.197 ± 0.637
3.856ArgLys: 3.856 ± 0.811
4.326ArgLeu: 4.326 ± 0.858
1.034ArgMet: 1.034 ± 0.246
3.667ArgAsn: 3.667 ± 0.418
1.505ArgPro: 1.505 ± 0.461
2.351ArgGln: 2.351 ± 0.691
2.445ArgArg: 2.445 ± 0.604
1.505ArgSer: 1.505 ± 0.36
3.197ArgThr: 3.197 ± 1.074
2.163ArgVal: 2.163 ± 0.571
0.846ArgTrp: 0.846 ± 0.284
2.727ArgTyr: 2.727 ± 0.593
0.0ArgXaa: 0.0 ± 0.0
Ser
5.454SerAla: 5.454 ± 1.276
0.282SerCys: 0.282 ± 0.136
2.915SerAsp: 2.915 ± 0.58
5.736SerGlu: 5.736 ± 1.056
3.197SerPhe: 3.197 ± 0.765
4.42SerGly: 4.42 ± 0.871
0.94SerHis: 0.94 ± 0.39
3.573SerIle: 3.573 ± 0.652
4.702SerLys: 4.702 ± 0.626
4.514SerLeu: 4.514 ± 0.524
1.317SerMet: 1.317 ± 0.477
3.291SerAsn: 3.291 ± 0.677
1.693SerPro: 1.693 ± 0.338
2.445SerGln: 2.445 ± 0.666
3.009SerArg: 3.009 ± 0.722
3.667SerSer: 3.667 ± 0.757
3.856SerThr: 3.856 ± 0.754
3.573SerVal: 3.573 ± 0.903
1.034SerTrp: 1.034 ± 0.337
2.257SerTyr: 2.257 ± 0.471
0.0SerXaa: 0.0 ± 0.0
Thr
4.796ThrAla: 4.796 ± 1.234
0.282ThrCys: 0.282 ± 0.185
4.984ThrAsp: 4.984 ± 0.964
4.044ThrGlu: 4.044 ± 0.612
3.667ThrPhe: 3.667 ± 1.125
4.232ThrGly: 4.232 ± 0.82
1.034ThrHis: 1.034 ± 0.322
4.608ThrIle: 4.608 ± 0.767
4.796ThrLys: 4.796 ± 0.618
4.984ThrLeu: 4.984 ± 0.658
0.658ThrMet: 0.658 ± 0.295
3.291ThrAsn: 3.291 ± 0.522
0.658ThrPro: 0.658 ± 0.207
2.727ThrGln: 2.727 ± 0.745
2.821ThrArg: 2.821 ± 0.399
3.856ThrSer: 3.856 ± 0.871
3.762ThrThr: 3.762 ± 0.876
4.796ThrVal: 4.796 ± 0.77
0.752ThrTrp: 0.752 ± 0.261
1.881ThrTyr: 1.881 ± 0.496
0.0ThrXaa: 0.0 ± 0.0
Val
2.915ValAla: 2.915 ± 0.543
0.282ValCys: 0.282 ± 0.175
3.103ValAsp: 3.103 ± 0.5
6.207ValGlu: 6.207 ± 0.812
2.445ValPhe: 2.445 ± 0.446
4.42ValGly: 4.42 ± 0.723
0.94ValHis: 0.94 ± 0.323
3.573ValIle: 3.573 ± 0.57
6.583ValLys: 6.583 ± 0.959
3.95ValLeu: 3.95 ± 0.776
1.599ValMet: 1.599 ± 0.437
3.009ValAsn: 3.009 ± 0.418
1.505ValPro: 1.505 ± 0.421
1.975ValGln: 1.975 ± 0.425
2.257ValArg: 2.257 ± 0.468
3.762ValSer: 3.762 ± 0.735
5.924ValThr: 5.924 ± 0.845
4.608ValVal: 4.608 ± 0.645
1.034ValTrp: 1.034 ± 0.393
1.505ValTyr: 1.505 ± 0.603
0.0ValXaa: 0.0 ± 0.0
Trp
0.564TrpAla: 0.564 ± 0.218
0.188TrpCys: 0.188 ± 0.149
0.282TrpAsp: 0.282 ± 0.188
1.128TrpGlu: 1.128 ± 0.432
0.376TrpPhe: 0.376 ± 0.198
0.752TrpGly: 0.752 ± 0.207
0.188TrpHis: 0.188 ± 0.148
1.599TrpIle: 1.599 ± 0.497
1.034TrpLys: 1.034 ± 0.363
1.034TrpLeu: 1.034 ± 0.3
0.376TrpMet: 0.376 ± 0.167
1.693TrpAsn: 1.693 ± 0.484
0.282TrpPro: 0.282 ± 0.176
0.846TrpGln: 0.846 ± 0.303
0.282TrpArg: 0.282 ± 0.158
0.846TrpSer: 0.846 ± 0.259
0.658TrpThr: 0.658 ± 0.211
0.94TrpVal: 0.94 ± 0.292
0.282TrpTrp: 0.282 ± 0.143
0.752TrpTyr: 0.752 ± 0.708
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.787TyrAla: 1.787 ± 0.389
0.564TyrCys: 0.564 ± 0.231
1.787TyrAsp: 1.787 ± 0.423
3.009TyrGlu: 3.009 ± 0.524
1.505TyrPhe: 1.505 ± 0.586
2.069TyrGly: 2.069 ± 0.443
0.564TyrHis: 0.564 ± 0.203
2.351TyrIle: 2.351 ± 0.404
1.881TyrLys: 1.881 ± 0.377
3.95TyrLeu: 3.95 ± 0.793
0.846TyrMet: 0.846 ± 0.369
1.317TyrAsn: 1.317 ± 0.47
1.599TyrPro: 1.599 ± 0.399
1.599TyrGln: 1.599 ± 0.529
2.257TyrArg: 2.257 ± 0.483
1.975TyrSer: 1.975 ± 0.45
1.317TyrThr: 1.317 ± 0.313
1.599TyrVal: 1.599 ± 0.35
0.47TyrTrp: 0.47 ± 0.198
1.975TyrTyr: 1.975 ± 0.948
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 48 proteins (10635 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski