Alteromonas phage ZP6
Average proteome isoelectric point is 5.98
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 48 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A7D7KLF8|A0A7D7KLF8_9CAUD Uncharacterized protein OS=Alteromonas phage ZP6 OX=2492447 PE=4 SV=1
MM1 pKa = 7.44 NNDD4 pKa = 3.04 ARR6 pKa = 11.84 NALNEE11 pKa = 4.23 ALDD14 pKa = 3.9 NAPVLGNAGRR24 pKa = 11.84 ALDD27 pKa = 4.13 GLLGLAQGSTLEE39 pKa = 4.79 SVLQDD44 pKa = 3.12 APSEE48 pKa = 4.03 YY49 pKa = 10.02 RR50 pKa = 11.84 EE51 pKa = 4.07 QAEE54 pKa = 3.59 AWYY57 pKa = 9.39 EE58 pKa = 3.8 EE59 pKa = 4.27 RR60 pKa = 11.84 VADD63 pKa = 6.13 GIAPEE68 pKa = 3.94 QALEE72 pKa = 4.12 EE73 pKa = 4.23 LEE75 pKa = 4.8 AWLGDD80 pKa = 3.95 CC81 pKa = 5.81
Molecular weight: 8.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.2
IPC2_protein 3.808
IPC_protein 3.694
Toseland 3.516
ProMoST 3.846
Dawson 3.668
Bjellqvist 3.821
Wikipedia 3.567
Rodwell 3.528
Grimsley 3.439
Solomon 3.63
Lehninger 3.592
Nozaki 3.795
DTASelect 3.91
Thurlkill 3.567
EMBOSS 3.579
Sillero 3.808
Patrickios 3.3
IPC_peptide 3.63
IPC2_peptide 3.783
IPC2.peptide.svr19 3.763
Protein with the highest isoelectric point:
>tr|A0A3S9U891|A0A3S9U891_9CAUD Adenylosuccinate synthetase OS=Alteromonas phage ZP6 OX=2492447 PE=4 SV=1
MM1 pKa = 6.76 STAFAKK7 pKa = 10.61 YY8 pKa = 8.94 KK9 pKa = 10.76 AKK11 pKa = 10.18 IRR13 pKa = 11.84 EE14 pKa = 4.01 AAKK17 pKa = 10.38 EE18 pKa = 3.85 GLSRR22 pKa = 11.84 NEE24 pKa = 4.22 LGRR27 pKa = 11.84 KK28 pKa = 9.28 SGVCAEE34 pKa = 4.88 TITAHH39 pKa = 6.64 LNRR42 pKa = 11.84 GRR44 pKa = 11.84 VPSILIAEE52 pKa = 5.02 TILNTLGYY60 pKa = 9.75 EE61 pKa = 3.66 IVIRR65 pKa = 11.84 KK66 pKa = 8.67 RR67 pKa = 11.84 KK68 pKa = 9.79 KK69 pKa = 9.02 II70 pKa = 3.59
Molecular weight: 7.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.251
IPC2_protein 9.619
IPC_protein 9.853
Toseland 10.643
ProMoST 10.175
Dawson 10.73
Bjellqvist 10.35
Wikipedia 10.862
Rodwell 11.213
Grimsley 10.76
Solomon 10.789
Lehninger 10.774
Nozaki 10.613
DTASelect 10.335
Thurlkill 10.628
EMBOSS 11.023
Sillero 10.643
Patrickios 10.979
IPC_peptide 10.789
IPC2_peptide 9.004
IPC2.peptide.svr19 8.652
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
48
0
48
11951
39
1203
249.0
27.75
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.045 ± 0.541
0.946 ± 0.143
6.359 ± 0.206
7.422 ± 0.383
3.305 ± 0.233
7.64 ± 0.525
1.64 ± 0.144
5.414 ± 0.174
5.606 ± 0.386
7.163 ± 0.3
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.996 ± 0.21
5.171 ± 0.287
3.431 ± 0.221
4.217 ± 0.238
5.631 ± 0.298
6.468 ± 0.354
5.974 ± 0.418
6.485 ± 0.327
1.757 ± 0.189
3.33 ± 0.151
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here