Gordonia phage Reyja

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 65 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6T816|A0A4D6T816_9CAUD Tape measure protein OS=Gordonia phage Reyja OX=2571250 GN=17 PE=4 SV=1
MM1 pKa = 7.02TLEE4 pKa = 4.17HH5 pKa = 6.6RR6 pKa = 11.84TTVTDD11 pKa = 3.32AAGRR15 pKa = 11.84LDD17 pKa = 5.05DD18 pKa = 6.32DD19 pKa = 4.59GEE21 pKa = 4.16NWTITLVDD29 pKa = 3.93PRR31 pKa = 11.84RR32 pKa = 11.84TAYY35 pKa = 10.26GIQRR39 pKa = 11.84VHH41 pKa = 6.08EE42 pKa = 4.09VDD44 pKa = 3.32PRR46 pKa = 11.84RR47 pKa = 11.84VPMHH51 pKa = 5.59VLEE54 pKa = 5.16AVMVWAHH61 pKa = 6.6AGLNPDD67 pKa = 4.29IDD69 pKa = 4.63DD70 pKa = 4.2ATDD73 pKa = 3.67PPTDD77 pKa = 3.64EE78 pKa = 6.02DD79 pKa = 4.39IIDD82 pKa = 3.86AEE84 pKa = 4.36IVDD87 pKa = 4.38DD88 pKa = 4.63AGFYY92 pKa = 10.71CVACGHH98 pKa = 6.17NAHH101 pKa = 7.26APGQCNTLVEE111 pKa = 4.52VTSGGVEE118 pKa = 3.87STDD121 pKa = 3.08ACRR124 pKa = 11.84CSVMQEE130 pKa = 3.94APRR133 pKa = 11.84SS134 pKa = 3.73

Molecular weight: 14.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6TAI5|A0A4D6TAI5_9CAUD Uncharacterized protein OS=Gordonia phage Reyja OX=2571250 GN=41 PE=4 SV=1
MM1 pKa = 7.69RR2 pKa = 11.84RR3 pKa = 11.84LEE5 pKa = 4.22TDD7 pKa = 3.34LGAPILTATTADD19 pKa = 3.6LAEE22 pKa = 4.24WLSRR26 pKa = 11.84DD27 pKa = 3.37DD28 pKa = 4.03LAPVTRR34 pKa = 11.84SVYY37 pKa = 10.65HH38 pKa = 6.44SMITGFFGWAISVGHH53 pKa = 6.56HH54 pKa = 6.64PGPNPMDD61 pKa = 5.47PIRR64 pKa = 11.84AAKK67 pKa = 9.86RR68 pKa = 11.84PRR70 pKa = 11.84RR71 pKa = 11.84QPRR74 pKa = 11.84PLTAAQYY81 pKa = 10.77RR82 pKa = 11.84RR83 pKa = 11.84LLDD86 pKa = 3.75AAGDD90 pKa = 3.93DD91 pKa = 3.6HH92 pKa = 8.93AMRR95 pKa = 11.84AMLLLAGMQGLRR107 pKa = 11.84VHH109 pKa = 7.1EE110 pKa = 4.08IARR113 pKa = 11.84FHH115 pKa = 6.7SRR117 pKa = 11.84HH118 pKa = 6.21LDD120 pKa = 3.31VEE122 pKa = 4.18ARR124 pKa = 11.84TLEE127 pKa = 4.18VVGKK131 pKa = 10.69GGTSYY136 pKa = 11.13LLPAASPVIEE146 pKa = 4.9HH147 pKa = 6.66ARR149 pKa = 11.84HH150 pKa = 5.23MPASGFWFPSQRR162 pKa = 11.84AKK164 pKa = 10.86HH165 pKa = 5.51LGGRR169 pKa = 11.84TVSEE173 pKa = 4.67RR174 pKa = 11.84IHH176 pKa = 7.64LHH178 pKa = 5.49MIRR181 pKa = 11.84SRR183 pKa = 11.84VPGTPHH189 pKa = 7.81ALRR192 pKa = 11.84HH193 pKa = 5.29TYY195 pKa = 8.43GTEE198 pKa = 3.71LVAGGTDD205 pKa = 3.26LRR207 pKa = 11.84VAQEE211 pKa = 4.05LLRR214 pKa = 11.84HH215 pKa = 5.97SSLQTTAIYY224 pKa = 10.28VAASDD229 pKa = 3.58EE230 pKa = 4.32RR231 pKa = 11.84KK232 pKa = 9.74RR233 pKa = 11.84DD234 pKa = 3.61AITRR238 pKa = 11.84LEE240 pKa = 4.09SLVAA244 pKa = 4.26

Molecular weight: 27.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

65

0

65

13596

41

1411

209.2

22.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.636 ± 0.715

0.647 ± 0.132

6.671 ± 0.311

5.936 ± 0.275

2.405 ± 0.18

8.841 ± 0.442

2.302 ± 0.237

4.435 ± 0.168

2.846 ± 0.19

7.642 ± 0.274

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.015 ± 0.145

3.148 ± 0.214

5.958 ± 0.299

3.016 ± 0.198

7.561 ± 0.453

5.09 ± 0.254

7.502 ± 0.306

7.296 ± 0.281

1.927 ± 0.153

2.126 ± 0.127
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski