Herpetosiphon aurantiacus (strain ATCC 23779 / DSM 785 / 114-95)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Chloroflexi; Chloroflexia; Herpetosiphonales; Herpetosiphonaceae; Herpetosiphon; Herpetosiphon aurantiacus

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5254 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A9B7N3|A9B7N3_HERA2 Uncharacterized protein OS=Herpetosiphon aurantiacus (strain ATCC 23779 / DSM 785 / 114-95) OX=316274 GN=Haur_0355 PE=4 SV=1
MM1 pKa = 8.07RR2 pKa = 11.84LRR4 pKa = 11.84IGFIGLMIALLGLTGGLRR22 pKa = 11.84SLTSVQAVPNAPQNLAAHH40 pKa = 5.8QLNTPQGFARR50 pKa = 11.84ISEE53 pKa = 4.3QEE55 pKa = 4.33SNDD58 pKa = 3.85TPAMAQPIIGDD69 pKa = 3.66SAVIRR74 pKa = 11.84GYY76 pKa = 8.6ITANDD81 pKa = 3.89SDD83 pKa = 4.31FLAIPLTSVQRR94 pKa = 11.84VVAATMTSASATNIVDD110 pKa = 4.69SILTLYY116 pKa = 10.41QPDD119 pKa = 3.85GTTVLEE125 pKa = 4.58LDD127 pKa = 3.78NDD129 pKa = 4.09DD130 pKa = 5.22GIFGVSSSVISSAIITTTATYY151 pKa = 9.77YY152 pKa = 11.19LKK154 pKa = 9.78VTANNTTNKK163 pKa = 9.53IYY165 pKa = 10.83YY166 pKa = 7.88YY167 pKa = 10.29DD168 pKa = 3.95LYY170 pKa = 11.62VRR172 pKa = 11.84ILTSEE177 pKa = 4.71PIVEE181 pKa = 4.13QEE183 pKa = 4.17PNEE186 pKa = 4.06PAQMLPAAGIVSGIISQTGDD206 pKa = 2.66IDD208 pKa = 4.26RR209 pKa = 11.84FQLALNPGDD218 pKa = 3.86TLFTTLDD225 pKa = 3.39IDD227 pKa = 3.9PEE229 pKa = 4.25RR230 pKa = 11.84DD231 pKa = 3.62GISWNGRR238 pKa = 11.84VTIGPFGPNIITINDD253 pKa = 3.6GNAVSPNAEE262 pKa = 4.19SAQLTVKK269 pKa = 10.32EE270 pKa = 4.1AGNYY274 pKa = 9.02IISVDD279 pKa = 3.59NLSAINNSTYY289 pKa = 10.54ILQVLIIPAEE299 pKa = 4.07EE300 pKa = 3.8QANCQTYY307 pKa = 9.55MSTDD311 pKa = 3.28VNKK314 pKa = 9.95TIPPDD319 pKa = 3.68PGMITSGLNIPDD331 pKa = 3.69NLLIGDD337 pKa = 5.06LDD339 pKa = 5.24LIIQLDD345 pKa = 3.69HH346 pKa = 6.53TFIPDD351 pKa = 4.11LDD353 pKa = 4.2AQLTTPDD360 pKa = 3.98GNILGLFSDD369 pKa = 4.12IGITTSAPASMNLIIDD385 pKa = 4.44DD386 pKa = 4.07EE387 pKa = 4.47AALPSFQALSVNGLINLPEE406 pKa = 3.96AAYY409 pKa = 10.59RR410 pKa = 11.84MSWFDD415 pKa = 3.43GQRR418 pKa = 11.84TQGNWTLTLYY428 pKa = 11.03DD429 pKa = 4.72DD430 pKa = 3.91ATGDD434 pKa = 4.69GGTLLNWGLQVCAALPPADD453 pKa = 4.79CPDD456 pKa = 3.52GTVSSTIYY464 pKa = 9.88STDD467 pKa = 3.38FEE469 pKa = 6.09ANDD472 pKa = 3.93GGFTHH477 pKa = 7.13SGTTDD482 pKa = 2.84PWQYY486 pKa = 10.64GQPNSAPIVGSYY498 pKa = 10.12SGSNSWKK505 pKa = 9.31TNLTGNYY512 pKa = 7.01PALMNANLTSPAIDD526 pKa = 3.45LSGVVGPIQVQWQQRR541 pKa = 11.84YY542 pKa = 8.76QIEE545 pKa = 4.17SAAFDD550 pKa = 3.51NYY552 pKa = 10.46AAQIVGSSNQVLFQHH567 pKa = 7.02RR568 pKa = 11.84DD569 pKa = 2.97GVMRR573 pKa = 11.84EE574 pKa = 4.24SVGNPLVTVQASTGWSRR591 pKa = 11.84QLHH594 pKa = 6.76DD595 pKa = 3.2ISSFVGQSIQLQFHH609 pKa = 7.0MDD611 pKa = 3.3TDD613 pKa = 4.14SSVEE617 pKa = 3.93LAGVAIDD624 pKa = 4.29DD625 pKa = 4.13VLVTGCVVLPTATPTEE641 pKa = 4.63TPTNTPTNTPTEE653 pKa = 4.5TPTNTPTVTVTPSNTPIVTPSVTAGPTTIPVYY685 pKa = 10.72LPLVSKK691 pKa = 11.49GEE693 pKa = 4.0

Molecular weight:
73.61 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A9AXS7|A9AXS7_HERA2 Uncharacterized protein OS=Herpetosiphon aurantiacus (strain ATCC 23779 / DSM 785 / 114-95) OX=316274 GN=Haur_2253 PE=4 SV=1
MM1 pKa = 8.05PKK3 pKa = 9.18RR4 pKa = 11.84TWQPKK9 pKa = 7.6RR10 pKa = 11.84IKK12 pKa = 9.86RR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84VHH17 pKa = 6.51GFLEE21 pKa = 4.43RR22 pKa = 11.84MQTRR26 pKa = 11.84TGRR29 pKa = 11.84AVLKK33 pKa = 10.54ARR35 pKa = 11.84RR36 pKa = 11.84QRR38 pKa = 11.84GRR40 pKa = 11.84WKK42 pKa = 9.76LTVSDD47 pKa = 3.88KK48 pKa = 10.8RR49 pKa = 11.84RR50 pKa = 11.84PAGPGGHH57 pKa = 6.3RR58 pKa = 3.81

Molecular weight:
7.0 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5254

0

5254

1962024

38

6661

373.4

41.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.671 ± 0.045

0.815 ± 0.01

5.036 ± 0.026

5.167 ± 0.031

3.443 ± 0.021

7.107 ± 0.031

2.247 ± 0.017

5.993 ± 0.031

2.629 ± 0.025

11.132 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.094 ± 0.015

3.704 ± 0.031

5.165 ± 0.031

5.541 ± 0.034

5.86 ± 0.031

5.883 ± 0.029

6.088 ± 0.039

6.775 ± 0.032

1.737 ± 0.016

2.913 ± 0.018

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski