Herpetosiphon aurantiacus (strain ATCC 23779 / DSM 785 / 114-95)
Average proteome isoelectric point is 6.19
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5254 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A9B7N3|A9B7N3_HERA2 Uncharacterized protein OS=Herpetosiphon aurantiacus (strain ATCC 23779 / DSM 785 / 114-95) OX=316274 GN=Haur_0355 PE=4 SV=1
MM1 pKa = 8.07 RR2 pKa = 11.84 LRR4 pKa = 11.84 IGFIGLMIALLGLTGGLRR22 pKa = 11.84 SLTSVQAVPNAPQNLAAHH40 pKa = 5.8 QLNTPQGFARR50 pKa = 11.84 ISEE53 pKa = 4.3 QEE55 pKa = 4.33 SNDD58 pKa = 3.85 TPAMAQPIIGDD69 pKa = 3.66 SAVIRR74 pKa = 11.84 GYY76 pKa = 8.6 ITANDD81 pKa = 3.89 SDD83 pKa = 4.31 FLAIPLTSVQRR94 pKa = 11.84 VVAATMTSASATNIVDD110 pKa = 4.69 SILTLYY116 pKa = 10.41 QPDD119 pKa = 3.85 GTTVLEE125 pKa = 4.58 LDD127 pKa = 3.78 NDD129 pKa = 4.09 DD130 pKa = 5.22 GIFGVSSSVISSAIITTTATYY151 pKa = 9.77 YY152 pKa = 11.19 LKK154 pKa = 9.78 VTANNTTNKK163 pKa = 9.53 IYY165 pKa = 10.83 YY166 pKa = 7.88 YY167 pKa = 10.29 DD168 pKa = 3.95 LYY170 pKa = 11.62 VRR172 pKa = 11.84 ILTSEE177 pKa = 4.71 PIVEE181 pKa = 4.13 QEE183 pKa = 4.17 PNEE186 pKa = 4.06 PAQMLPAAGIVSGIISQTGDD206 pKa = 2.66 IDD208 pKa = 4.26 RR209 pKa = 11.84 FQLALNPGDD218 pKa = 3.86 TLFTTLDD225 pKa = 3.39 IDD227 pKa = 3.9 PEE229 pKa = 4.25 RR230 pKa = 11.84 DD231 pKa = 3.62 GISWNGRR238 pKa = 11.84 VTIGPFGPNIITINDD253 pKa = 3.6 GNAVSPNAEE262 pKa = 4.19 SAQLTVKK269 pKa = 10.32 EE270 pKa = 4.1 AGNYY274 pKa = 9.02 IISVDD279 pKa = 3.59 NLSAINNSTYY289 pKa = 10.54 ILQVLIIPAEE299 pKa = 4.07 EE300 pKa = 3.8 QANCQTYY307 pKa = 9.55 MSTDD311 pKa = 3.28 VNKK314 pKa = 9.95 TIPPDD319 pKa = 3.68 PGMITSGLNIPDD331 pKa = 3.69 NLLIGDD337 pKa = 5.06 LDD339 pKa = 5.24 LIIQLDD345 pKa = 3.69 HH346 pKa = 6.53 TFIPDD351 pKa = 4.11 LDD353 pKa = 4.2 AQLTTPDD360 pKa = 3.98 GNILGLFSDD369 pKa = 4.12 IGITTSAPASMNLIIDD385 pKa = 4.44 DD386 pKa = 4.07 EE387 pKa = 4.47 AALPSFQALSVNGLINLPEE406 pKa = 3.96 AAYY409 pKa = 10.59 RR410 pKa = 11.84 MSWFDD415 pKa = 3.43 GQRR418 pKa = 11.84 TQGNWTLTLYY428 pKa = 11.03 DD429 pKa = 4.72 DD430 pKa = 3.91 ATGDD434 pKa = 4.69 GGTLLNWGLQVCAALPPADD453 pKa = 4.79 CPDD456 pKa = 3.52 GTVSSTIYY464 pKa = 9.88 STDD467 pKa = 3.38 FEE469 pKa = 6.09 ANDD472 pKa = 3.93 GGFTHH477 pKa = 7.13 SGTTDD482 pKa = 2.84 PWQYY486 pKa = 10.64 GQPNSAPIVGSYY498 pKa = 10.12 SGSNSWKK505 pKa = 9.31 TNLTGNYY512 pKa = 7.01 PALMNANLTSPAIDD526 pKa = 3.45 LSGVVGPIQVQWQQRR541 pKa = 11.84 YY542 pKa = 8.76 QIEE545 pKa = 4.17 SAAFDD550 pKa = 3.51 NYY552 pKa = 10.46 AAQIVGSSNQVLFQHH567 pKa = 7.02 RR568 pKa = 11.84 DD569 pKa = 2.97 GVMRR573 pKa = 11.84 EE574 pKa = 4.24 SVGNPLVTVQASTGWSRR591 pKa = 11.84 QLHH594 pKa = 6.76 DD595 pKa = 3.2 ISSFVGQSIQLQFHH609 pKa = 7.0 MDD611 pKa = 3.3 TDD613 pKa = 4.14 SSVEE617 pKa = 3.93 LAGVAIDD624 pKa = 4.29 DD625 pKa = 4.13 VLVTGCVVLPTATPTEE641 pKa = 4.63 TPTNTPTNTPTEE653 pKa = 4.5 TPTNTPTVTVTPSNTPIVTPSVTAGPTTIPVYY685 pKa = 10.72 LPLVSKK691 pKa = 11.49 GEE693 pKa = 4.0
Molecular weight: 73.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.693
IPC2_protein 3.834
IPC_protein 3.872
Toseland 3.63
ProMoST 4.024
Dawson 3.859
Bjellqvist 4.037
Wikipedia 3.808
Rodwell 3.681
Grimsley 3.541
Solomon 3.859
Lehninger 3.821
Nozaki 3.973
DTASelect 4.24
Thurlkill 3.694
EMBOSS 3.821
Sillero 3.986
Patrickios 1.418
IPC_peptide 3.859
IPC2_peptide 3.961
IPC2.peptide.svr19 3.882
Protein with the highest isoelectric point:
>tr|A9AXS7|A9AXS7_HERA2 Uncharacterized protein OS=Herpetosiphon aurantiacus (strain ATCC 23779 / DSM 785 / 114-95) OX=316274 GN=Haur_2253 PE=4 SV=1
MM1 pKa = 8.05 PKK3 pKa = 9.18 RR4 pKa = 11.84 TWQPKK9 pKa = 7.6 RR10 pKa = 11.84 IKK12 pKa = 9.86 RR13 pKa = 11.84 RR14 pKa = 11.84 RR15 pKa = 11.84 VHH17 pKa = 6.51 GFLEE21 pKa = 4.43 RR22 pKa = 11.84 MQTRR26 pKa = 11.84 TGRR29 pKa = 11.84 AVLKK33 pKa = 10.54 ARR35 pKa = 11.84 RR36 pKa = 11.84 QRR38 pKa = 11.84 GRR40 pKa = 11.84 WKK42 pKa = 9.76 LTVSDD47 pKa = 3.88 KK48 pKa = 10.8 RR49 pKa = 11.84 RR50 pKa = 11.84 PAGPGGHH57 pKa = 6.3 RR58 pKa = 3.81
Molecular weight: 7.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.398
IPC2_protein 10.847
IPC_protein 12.442
Toseland 12.603
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.281
Grimsley 12.647
Solomon 13.1
Lehninger 13.013
Nozaki 12.603
DTASelect 12.603
Thurlkill 12.603
EMBOSS 13.1
Sillero 12.603
Patrickios 12.003
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.102
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5254
0
5254
1962024
38
6661
373.4
41.12
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.671 ± 0.045
0.815 ± 0.01
5.036 ± 0.026
5.167 ± 0.031
3.443 ± 0.021
7.107 ± 0.031
2.247 ± 0.017
5.993 ± 0.031
2.629 ± 0.025
11.132 ± 0.04
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.094 ± 0.015
3.704 ± 0.031
5.165 ± 0.031
5.541 ± 0.034
5.86 ± 0.031
5.883 ± 0.029
6.088 ± 0.039
6.775 ± 0.032
1.737 ± 0.016
2.913 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here