Agrococcus casei LMG 22410

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Agrococcus; Agrococcus casei

Average proteome isoelectric point is 5.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2698 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1R4GJN6|A0A1R4GJN6_9MICO Chromosome (Plasmid) partitioning protein ParA OS=Agrococcus casei LMG 22410 OX=1255656 GN=CZ674_12595 PE=4 SV=1
MM1 pKa = 7.0IHH3 pKa = 6.46VRR5 pKa = 11.84TLTKK9 pKa = 10.56KK10 pKa = 10.48LGIVALSATVAASGLTAITPAPAAQAAPSTGLVISEE46 pKa = 4.51AYY48 pKa = 10.29GGGGNGGAKK57 pKa = 10.12FNQDD61 pKa = 3.72FIEE64 pKa = 4.97LYY66 pKa = 9.74NFSAAEE72 pKa = 3.89VSLDD76 pKa = 3.26GVTVSYY82 pKa = 10.85YY83 pKa = 10.69SSSGNLGASTEE94 pKa = 4.49LEE96 pKa = 4.25GSLQPGEE103 pKa = 4.55HH104 pKa = 6.29YY105 pKa = 10.87LVGAAYY111 pKa = 10.04GSNRR115 pKa = 11.84DD116 pKa = 3.61LPSFEE121 pKa = 5.13SDD123 pKa = 2.83ATFNAAMSGSKK134 pKa = 10.69GSVEE138 pKa = 3.68IAAGDD143 pKa = 3.83TVIDD147 pKa = 3.86LVGWGDD153 pKa = 3.19AALFEE158 pKa = 4.73GAPAHH163 pKa = 5.57GTSNSVSVARR173 pKa = 11.84VDD175 pKa = 3.81PASDD179 pKa = 3.39TDD181 pKa = 4.06DD182 pKa = 3.91NSVDD186 pKa = 3.96FATGEE191 pKa = 4.01PTPQGLAEE199 pKa = 5.64DD200 pKa = 4.47GTEE203 pKa = 4.3EE204 pKa = 4.71PPVDD208 pKa = 4.05PPADD212 pKa = 3.87PEE214 pKa = 4.14TATISEE220 pKa = 4.25IQGEE224 pKa = 4.49GAEE227 pKa = 4.62TPFMDD232 pKa = 4.94QPVITQGIVTAVYY245 pKa = 9.01TGQGSKK251 pKa = 10.75NGFNIQEE258 pKa = 4.37PGVVDD263 pKa = 4.04IASHH267 pKa = 5.92AASTGVFVYY276 pKa = 10.34GSSHH280 pKa = 6.59AEE282 pKa = 3.78SVQIGDD288 pKa = 3.71SVEE291 pKa = 3.97VHH293 pKa = 5.61GTATEE298 pKa = 4.47YY299 pKa = 11.53YY300 pKa = 10.25DD301 pKa = 3.45VTQIEE306 pKa = 4.42ASSVTALGEE315 pKa = 4.34SLGEE319 pKa = 3.89ATAVEE324 pKa = 4.24LAEE327 pKa = 4.18WPTEE331 pKa = 4.01EE332 pKa = 4.18EE333 pKa = 3.84VRR335 pKa = 11.84EE336 pKa = 4.13LFEE339 pKa = 5.34GMLLAPQGTYY349 pKa = 8.7TVSNNYY355 pKa = 10.11ALNQYY360 pKa = 10.54GEE362 pKa = 4.31VGLAFGDD369 pKa = 4.0SALVQPTEE377 pKa = 4.02VGAPGSDD384 pKa = 2.91AAYY387 pKa = 10.22AQAEE391 pKa = 4.3ANAAASVLLDD401 pKa = 5.59DD402 pKa = 4.8GQTWDD407 pKa = 4.01YY408 pKa = 10.1MSNDD412 pKa = 3.52AAKK415 pKa = 10.39DD416 pKa = 3.55SPLPYY421 pKa = 9.35LTLEE425 pKa = 4.22TPVTIGAVAAFTDD438 pKa = 4.89GVVLDD443 pKa = 4.59YY444 pKa = 11.76SHH446 pKa = 6.34GTWRR450 pKa = 11.84FQPTQPVHH458 pKa = 5.05GTEE461 pKa = 3.93NAPVAFSDD469 pKa = 3.74VRR471 pKa = 11.84EE472 pKa = 4.04QAPEE476 pKa = 3.95DD477 pKa = 3.51VGGDD481 pKa = 3.23ITIGTFNVLNYY492 pKa = 7.4FTTLGDD498 pKa = 3.68TEE500 pKa = 4.86AGCEE504 pKa = 3.93AYY506 pKa = 9.79EE507 pKa = 4.9DD508 pKa = 4.4RR509 pKa = 11.84DD510 pKa = 3.96GNPIATDD517 pKa = 3.39YY518 pKa = 7.54CTPRR522 pKa = 11.84GAYY525 pKa = 9.6DD526 pKa = 3.24AEE528 pKa = 4.19NFEE531 pKa = 4.28RR532 pKa = 11.84QQAKK536 pKa = 9.68IVTAINTLDD545 pKa = 3.49ASVVALEE552 pKa = 4.69EE553 pKa = 4.57IEE555 pKa = 4.83DD556 pKa = 3.9SSDD559 pKa = 3.27FGNDD563 pKa = 2.42RR564 pKa = 11.84DD565 pKa = 3.7ASIEE569 pKa = 4.07HH570 pKa = 6.55LVGEE574 pKa = 4.69LNAAADD580 pKa = 3.94EE581 pKa = 4.47DD582 pKa = 3.65RR583 pKa = 11.84WAFVPSPEE591 pKa = 4.52WIPATGDD598 pKa = 3.2DD599 pKa = 4.56VIRR602 pKa = 11.84TGYY605 pKa = 9.32IYY607 pKa = 10.77KK608 pKa = 9.67PAEE611 pKa = 3.58VSYY614 pKa = 11.08IEE616 pKa = 4.84GSAEE620 pKa = 4.09LLDD623 pKa = 4.71DD624 pKa = 4.67AAFEE628 pKa = 4.07NARR631 pKa = 11.84APFAALFAPADD642 pKa = 3.72EE643 pKa = 4.62ALSGNEE649 pKa = 3.51FLVIANHH656 pKa = 6.31FKK658 pKa = 11.12SKK660 pKa = 10.52GGSGDD665 pKa = 3.27GDD667 pKa = 3.48NANRR671 pKa = 11.84DD672 pKa = 3.92DD673 pKa = 4.38EE674 pKa = 5.38LGPAHH679 pKa = 7.26AVGGWNGDD687 pKa = 3.17RR688 pKa = 11.84VRR690 pKa = 11.84QAEE693 pKa = 4.26AVVEE697 pKa = 4.38FADD700 pKa = 4.5TLEE703 pKa = 4.26QQHH706 pKa = 5.73GTDD709 pKa = 3.86LVFITGDD716 pKa = 3.54LNSYY720 pKa = 9.17AQEE723 pKa = 4.48DD724 pKa = 4.6PVTTILNAGYY734 pKa = 8.77TNLTAGGPEE743 pKa = 3.84YY744 pKa = 10.75SYY746 pKa = 11.74AFGGMVGSLDD756 pKa = 3.26HH757 pKa = 6.64VLASEE762 pKa = 4.18AATALVTGADD772 pKa = 3.28LWTINAYY779 pKa = 10.06EE780 pKa = 4.14PVALEE785 pKa = 4.07YY786 pKa = 10.77SRR788 pKa = 11.84HH789 pKa = 5.51NYY791 pKa = 9.11NVAQLYY797 pKa = 10.36AADD800 pKa = 3.83QFRR803 pKa = 11.84SSDD806 pKa = 3.64HH807 pKa = 6.34NPALVGVSFAEE818 pKa = 4.7DD819 pKa = 3.5DD820 pKa = 5.05DD821 pKa = 5.18IEE823 pKa = 5.96ADD825 pKa = 3.68EE826 pKa = 4.6PLLVIEE832 pKa = 4.37QEE834 pKa = 4.34QYY836 pKa = 9.58TQTEE840 pKa = 4.62SIDD843 pKa = 3.79GVSYY847 pKa = 10.73AATGLRR853 pKa = 11.84EE854 pKa = 3.97GTAVVEE860 pKa = 4.86LIDD863 pKa = 3.97AGGSVSVIEE872 pKa = 4.87GAAQTEE878 pKa = 4.29AGALAGEE885 pKa = 4.37VSYY888 pKa = 8.88EE889 pKa = 4.07TEE891 pKa = 3.63AGEE894 pKa = 4.3RR895 pKa = 11.84LRR897 pKa = 11.84MPVGTYY903 pKa = 7.07TLRR906 pKa = 11.84VTQEE910 pKa = 3.71GSGGAEE916 pKa = 3.92SIVLEE921 pKa = 4.09AAFEE925 pKa = 4.32VVADD929 pKa = 3.95EE930 pKa = 4.77TGDD933 pKa = 4.69DD934 pKa = 3.84GTGDD938 pKa = 4.0DD939 pKa = 4.69GSFDD943 pKa = 3.92GDD945 pKa = 3.25EE946 pKa = 5.1DD947 pKa = 4.17GQPDD951 pKa = 3.84LPEE954 pKa = 4.15TGFDD958 pKa = 3.15GGLAPFVLALALMLGGTGFVLLRR981 pKa = 11.84RR982 pKa = 11.84RR983 pKa = 11.84NAA985 pKa = 3.24

Molecular weight: 102.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1R4ERB9|A0A1R4ERB9_9MICO Uncharacterized protein OS=Agrococcus casei LMG 22410 OX=1255656 GN=CZ674_00375 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 9.97KK16 pKa = 9.33HH17 pKa = 4.25GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILAARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.74GRR40 pKa = 11.84SSVSAA45 pKa = 3.76

Molecular weight: 5.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2698

0

2698

827534

37

3196

306.7

33.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.571 ± 0.067

0.483 ± 0.011

6.297 ± 0.046

6.41 ± 0.052

3.181 ± 0.034

8.588 ± 0.041

2.095 ± 0.022

4.917 ± 0.035

2.469 ± 0.038

9.724 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.199 ± 0.023

2.198 ± 0.03

4.843 ± 0.039

3.436 ± 0.027

6.705 ± 0.061

5.991 ± 0.031

6.0 ± 0.042

8.545 ± 0.041

1.43 ± 0.025

1.917 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski