Amino acid dipepetide frequency for Dickeya phage Amaethon

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.724AlaAla: 9.724 ± 1.818
0.723AlaCys: 0.723 ± 0.244
4.902AlaAsp: 4.902 ± 0.619
6.831AlaGlu: 6.831 ± 0.861
2.331AlaPhe: 2.331 ± 0.339
5.063AlaGly: 5.063 ± 0.86
1.527AlaHis: 1.527 ± 0.367
5.626AlaIle: 5.626 ± 0.711
6.108AlaLys: 6.108 ± 0.847
9.001AlaLeu: 9.001 ± 0.831
2.25AlaMet: 2.25 ± 0.363
5.545AlaAsn: 5.545 ± 0.706
2.17AlaPro: 2.17 ± 0.375
3.777AlaGln: 3.777 ± 0.726
4.661AlaArg: 4.661 ± 0.535
5.385AlaSer: 5.385 ± 0.901
5.867AlaThr: 5.867 ± 0.683
6.108AlaVal: 6.108 ± 0.693
1.768AlaTrp: 1.768 ± 0.411
2.893AlaTyr: 2.893 ± 0.582
0.0AlaXaa: 0.0 ± 0.0
Cys
1.045CysAla: 1.045 ± 0.315
0.08CysCys: 0.08 ± 0.096
0.643CysAsp: 0.643 ± 0.241
0.402CysGlu: 0.402 ± 0.173
0.482CysPhe: 0.482 ± 0.175
0.241CysGly: 0.241 ± 0.124
0.241CysHis: 0.241 ± 0.143
0.321CysIle: 0.321 ± 0.162
0.643CysLys: 0.643 ± 0.254
1.205CysLeu: 1.205 ± 0.281
0.08CysMet: 0.08 ± 0.087
0.402CysAsn: 0.402 ± 0.16
0.321CysPro: 0.321 ± 0.187
0.321CysGln: 0.321 ± 0.153
0.241CysArg: 0.241 ± 0.125
0.804CysSer: 0.804 ± 0.221
0.321CysThr: 0.321 ± 0.163
0.643CysVal: 0.643 ± 0.21
0.0CysTrp: 0.0 ± 0.0
0.482CysTyr: 0.482 ± 0.184
0.0CysXaa: 0.0 ± 0.0
Asp
5.224AspAla: 5.224 ± 0.611
0.964AspCys: 0.964 ± 0.274
3.616AspAsp: 3.616 ± 0.536
3.858AspGlu: 3.858 ± 0.508
2.17AspPhe: 2.17 ± 0.358
5.063AspGly: 5.063 ± 0.666
0.321AspHis: 0.321 ± 0.168
4.501AspIle: 4.501 ± 0.526
3.616AspLys: 3.616 ± 0.558
5.063AspLeu: 5.063 ± 0.411
1.848AspMet: 1.848 ± 0.341
2.411AspAsn: 2.411 ± 0.287
3.215AspPro: 3.215 ± 0.592
1.447AspGln: 1.447 ± 0.284
2.25AspArg: 2.25 ± 0.342
5.143AspSer: 5.143 ± 0.55
3.616AspThr: 3.616 ± 0.424
4.501AspVal: 4.501 ± 0.558
1.125AspTrp: 1.125 ± 0.294
3.616AspTyr: 3.616 ± 0.359
0.0AspXaa: 0.0 ± 0.0
Glu
5.465GluAla: 5.465 ± 0.516
0.482GluCys: 0.482 ± 0.171
4.34GluAsp: 4.34 ± 0.551
3.616GluGlu: 3.616 ± 0.515
2.17GluPhe: 2.17 ± 0.402
3.697GluGly: 3.697 ± 0.632
0.804GluHis: 0.804 ± 0.276
2.411GluIle: 2.411 ± 0.387
2.893GluLys: 2.893 ± 0.541
6.429GluLeu: 6.429 ± 0.818
1.607GluMet: 1.607 ± 0.403
2.974GluAsn: 2.974 ± 0.55
1.527GluPro: 1.527 ± 0.345
2.652GluGln: 2.652 ± 0.487
2.974GluArg: 2.974 ± 0.455
2.411GluSer: 2.411 ± 0.392
4.018GluThr: 4.018 ± 0.564
4.983GluVal: 4.983 ± 0.646
0.804GluTrp: 0.804 ± 0.223
3.134GluTyr: 3.134 ± 0.433
0.0GluXaa: 0.0 ± 0.0
Phe
1.848PheAla: 1.848 ± 0.343
0.482PheCys: 0.482 ± 0.178
2.572PheAsp: 2.572 ± 0.538
1.688PheGlu: 1.688 ± 0.397
0.723PhePhe: 0.723 ± 0.243
3.134PheGly: 3.134 ± 0.472
0.402PheHis: 0.402 ± 0.17
2.411PheIle: 2.411 ± 0.536
2.974PheLys: 2.974 ± 0.479
2.652PheLeu: 2.652 ± 0.374
0.723PheMet: 0.723 ± 0.192
2.893PheAsn: 2.893 ± 0.43
0.884PhePro: 0.884 ± 0.238
1.125PheGln: 1.125 ± 0.257
1.125PheArg: 1.125 ± 0.225
3.054PheSer: 3.054 ± 0.446
2.331PheThr: 2.331 ± 0.506
1.848PheVal: 1.848 ± 0.354
0.402PheTrp: 0.402 ± 0.188
1.045PheTyr: 1.045 ± 0.313
0.0PheXaa: 0.0 ± 0.0
Gly
5.143GlyAla: 5.143 ± 0.956
0.321GlyCys: 0.321 ± 0.17
3.215GlyAsp: 3.215 ± 0.655
3.375GlyGlu: 3.375 ± 0.504
2.813GlyPhe: 2.813 ± 0.421
4.581GlyGly: 4.581 ± 0.651
0.884GlyHis: 0.884 ± 0.272
4.501GlyIle: 4.501 ± 0.573
4.259GlyLys: 4.259 ± 0.582
5.063GlyLeu: 5.063 ± 0.675
1.205GlyMet: 1.205 ± 0.322
4.179GlyAsn: 4.179 ± 0.599
2.17GlyPro: 2.17 ± 0.434
2.491GlyGln: 2.491 ± 0.5
2.572GlyArg: 2.572 ± 0.397
3.777GlySer: 3.777 ± 0.549
5.304GlyThr: 5.304 ± 0.85
4.902GlyVal: 4.902 ± 0.607
1.527GlyTrp: 1.527 ± 0.347
2.813GlyTyr: 2.813 ± 0.595
0.0GlyXaa: 0.0 ± 0.0
His
1.205HisAla: 1.205 ± 0.272
0.321HisCys: 0.321 ± 0.157
0.402HisAsp: 0.402 ± 0.199
0.563HisGlu: 0.563 ± 0.197
0.964HisPhe: 0.964 ± 0.342
0.884HisGly: 0.884 ± 0.237
0.402HisHis: 0.402 ± 0.181
0.884HisIle: 0.884 ± 0.282
1.205HisLys: 1.205 ± 0.36
1.366HisLeu: 1.366 ± 0.541
0.402HisMet: 0.402 ± 0.154
1.205HisAsn: 1.205 ± 0.34
0.723HisPro: 0.723 ± 0.196
0.482HisGln: 0.482 ± 0.287
0.482HisArg: 0.482 ± 0.179
0.482HisSer: 0.482 ± 0.175
1.286HisThr: 1.286 ± 0.295
0.643HisVal: 0.643 ± 0.216
0.241HisTrp: 0.241 ± 0.141
1.366HisTyr: 1.366 ± 0.378
0.0HisXaa: 0.0 ± 0.0
Ile
5.706IleAla: 5.706 ± 0.752
0.482IleCys: 0.482 ± 0.173
3.938IleAsp: 3.938 ± 0.514
4.34IleGlu: 4.34 ± 0.66
1.205IlePhe: 1.205 ± 0.204
3.375IleGly: 3.375 ± 0.476
0.563IleHis: 0.563 ± 0.181
3.938IleIle: 3.938 ± 0.585
3.536IleLys: 3.536 ± 0.512
3.295IleLeu: 3.295 ± 0.628
1.366IleMet: 1.366 ± 0.4
3.456IleAsn: 3.456 ± 0.61
2.25IlePro: 2.25 ± 0.408
1.286IleGln: 1.286 ± 0.288
3.054IleArg: 3.054 ± 0.401
4.34IleSer: 4.34 ± 0.537
4.259IleThr: 4.259 ± 0.519
2.974IleVal: 2.974 ± 0.496
0.643IleTrp: 0.643 ± 0.216
2.17IleTyr: 2.17 ± 0.438
0.0IleXaa: 0.0 ± 0.0
Lys
6.269LysAla: 6.269 ± 0.96
0.482LysCys: 0.482 ± 0.19
5.385LysAsp: 5.385 ± 0.601
3.777LysGlu: 3.777 ± 0.578
2.17LysPhe: 2.17 ± 0.355
3.777LysGly: 3.777 ± 0.554
1.286LysHis: 1.286 ± 0.337
2.572LysIle: 2.572 ± 0.482
3.054LysLys: 3.054 ± 0.41
6.67LysLeu: 6.67 ± 0.66
1.286LysMet: 1.286 ± 0.228
2.652LysAsn: 2.652 ± 0.503
2.09LysPro: 2.09 ± 0.483
3.054LysGln: 3.054 ± 0.492
2.652LysArg: 2.652 ± 0.38
3.616LysSer: 3.616 ± 0.475
3.536LysThr: 3.536 ± 0.595
4.501LysVal: 4.501 ± 0.519
0.964LysTrp: 0.964 ± 0.275
1.848LysTyr: 1.848 ± 0.399
0.0LysXaa: 0.0 ± 0.0
Leu
7.876LeuAla: 7.876 ± 0.96
0.723LeuCys: 0.723 ± 0.281
6.429LeuAsp: 6.429 ± 0.751
6.429LeuGlu: 6.429 ± 0.671
2.25LeuPhe: 2.25 ± 0.519
5.224LeuGly: 5.224 ± 0.636
1.768LeuHis: 1.768 ± 0.329
4.179LeuIle: 4.179 ± 0.651
5.304LeuLys: 5.304 ± 0.544
6.831LeuLeu: 6.831 ± 0.763
2.491LeuMet: 2.491 ± 0.495
4.983LeuAsn: 4.983 ± 0.645
2.813LeuPro: 2.813 ± 0.471
4.661LeuGln: 4.661 ± 0.504
3.697LeuArg: 3.697 ± 0.506
6.269LeuSer: 6.269 ± 0.828
6.188LeuThr: 6.188 ± 0.475
5.867LeuVal: 5.867 ± 0.606
1.125LeuTrp: 1.125 ± 0.361
2.813LeuTyr: 2.813 ± 0.514
0.0LeuXaa: 0.0 ± 0.0
Met
3.054MetAla: 3.054 ± 0.421
0.08MetCys: 0.08 ± 0.077
1.205MetAsp: 1.205 ± 0.301
0.884MetGlu: 0.884 ± 0.243
0.482MetPhe: 0.482 ± 0.175
1.205MetGly: 1.205 ± 0.258
0.804MetHis: 0.804 ± 0.255
1.045MetIle: 1.045 ± 0.415
1.607MetLys: 1.607 ± 0.344
1.848MetLeu: 1.848 ± 0.342
0.482MetMet: 0.482 ± 0.197
1.045MetAsn: 1.045 ± 0.271
1.205MetPro: 1.205 ± 0.36
2.009MetGln: 2.009 ± 0.402
0.964MetArg: 0.964 ± 0.279
2.25MetSer: 2.25 ± 0.484
1.688MetThr: 1.688 ± 0.426
0.723MetVal: 0.723 ± 0.193
0.08MetTrp: 0.08 ± 0.09
1.205MetTyr: 1.205 ± 0.308
0.0MetXaa: 0.0 ± 0.0
Asn
4.179AsnAla: 4.179 ± 0.637
0.321AsnCys: 0.321 ± 0.178
2.491AsnAsp: 2.491 ± 0.33
2.652AsnGlu: 2.652 ± 0.531
2.17AsnPhe: 2.17 ± 0.447
3.777AsnGly: 3.777 ± 0.688
0.402AsnHis: 0.402 ± 0.214
3.295AsnIle: 3.295 ± 0.508
3.938AsnLys: 3.938 ± 0.627
4.581AsnLeu: 4.581 ± 0.682
1.366AsnMet: 1.366 ± 0.319
2.893AsnAsn: 2.893 ± 0.629
2.25AsnPro: 2.25 ± 0.377
2.09AsnGln: 2.09 ± 0.324
2.17AsnArg: 2.17 ± 0.449
3.536AsnSer: 3.536 ± 0.519
3.616AsnThr: 3.616 ± 0.43
3.456AsnVal: 3.456 ± 0.473
0.723AsnTrp: 0.723 ± 0.217
2.732AsnTyr: 2.732 ± 0.45
0.0AsnXaa: 0.0 ± 0.0
Pro
3.215ProAla: 3.215 ± 0.525
0.643ProCys: 0.643 ± 0.292
2.572ProAsp: 2.572 ± 0.472
2.009ProGlu: 2.009 ± 0.344
0.964ProPhe: 0.964 ± 0.265
1.527ProGly: 1.527 ± 0.3
0.241ProHis: 0.241 ± 0.151
1.929ProIle: 1.929 ± 0.427
2.893ProLys: 2.893 ± 0.444
2.09ProLeu: 2.09 ± 0.417
0.643ProMet: 0.643 ± 0.318
1.205ProAsn: 1.205 ± 0.357
0.964ProPro: 0.964 ± 0.311
1.205ProGln: 1.205 ± 0.334
1.768ProArg: 1.768 ± 0.337
2.652ProSer: 2.652 ± 0.42
2.732ProThr: 2.732 ± 0.493
3.375ProVal: 3.375 ± 0.551
0.402ProTrp: 0.402 ± 0.14
1.205ProTyr: 1.205 ± 0.304
0.0ProXaa: 0.0 ± 0.0
Gln
5.063GlnAla: 5.063 ± 0.798
0.321GlnCys: 0.321 ± 0.142
1.929GlnAsp: 1.929 ± 0.381
2.652GlnGlu: 2.652 ± 0.61
1.366GlnPhe: 1.366 ± 0.508
2.411GlnGly: 2.411 ± 0.385
0.884GlnHis: 0.884 ± 0.242
2.009GlnIle: 2.009 ± 0.329
1.768GlnLys: 1.768 ± 0.305
5.465GlnLeu: 5.465 ± 0.5
1.286GlnMet: 1.286 ± 0.312
1.688GlnAsn: 1.688 ± 0.352
1.447GlnPro: 1.447 ± 0.32
3.375GlnGln: 3.375 ± 0.541
1.607GlnArg: 1.607 ± 0.444
2.652GlnSer: 2.652 ± 0.435
2.732GlnThr: 2.732 ± 0.408
2.652GlnVal: 2.652 ± 0.454
0.723GlnTrp: 0.723 ± 0.311
2.17GlnTyr: 2.17 ± 0.363
0.0GlnXaa: 0.0 ± 0.0
Arg
3.938ArgAla: 3.938 ± 0.571
0.08ArgCys: 0.08 ± 0.09
2.17ArgAsp: 2.17 ± 0.42
2.572ArgGlu: 2.572 ± 0.518
2.009ArgPhe: 2.009 ± 0.389
2.732ArgGly: 2.732 ± 0.348
0.884ArgHis: 0.884 ± 0.253
3.215ArgIle: 3.215 ± 0.385
2.491ArgLys: 2.491 ± 0.515
3.697ArgLeu: 3.697 ± 0.518
0.964ArgMet: 0.964 ± 0.233
2.09ArgAsn: 2.09 ± 0.367
1.447ArgPro: 1.447 ± 0.295
2.893ArgGln: 2.893 ± 0.48
2.009ArgArg: 2.009 ± 0.448
2.411ArgSer: 2.411 ± 0.396
2.17ArgThr: 2.17 ± 0.379
2.652ArgVal: 2.652 ± 0.432
0.804ArgTrp: 0.804 ± 0.289
2.732ArgTyr: 2.732 ± 0.601
0.0ArgXaa: 0.0 ± 0.0
Ser
7.233SerAla: 7.233 ± 0.895
0.482SerCys: 0.482 ± 0.181
4.822SerAsp: 4.822 ± 0.577
3.375SerGlu: 3.375 ± 0.397
2.974SerPhe: 2.974 ± 0.556
5.947SerGly: 5.947 ± 0.718
0.804SerHis: 0.804 ± 0.287
2.813SerIle: 2.813 ± 0.465
3.697SerLys: 3.697 ± 0.458
5.465SerLeu: 5.465 ± 0.632
1.768SerMet: 1.768 ± 0.425
3.456SerAsn: 3.456 ± 0.424
2.732SerPro: 2.732 ± 0.351
2.491SerGln: 2.491 ± 0.43
2.411SerArg: 2.411 ± 0.415
4.581SerSer: 4.581 ± 0.897
4.983SerThr: 4.983 ± 0.563
3.375SerVal: 3.375 ± 0.576
0.804SerTrp: 0.804 ± 0.284
2.009SerTyr: 2.009 ± 0.344
0.0SerXaa: 0.0 ± 0.0
Thr
6.51ThrAla: 6.51 ± 0.68
0.563ThrCys: 0.563 ± 0.227
4.259ThrAsp: 4.259 ± 0.581
3.375ThrGlu: 3.375 ± 0.401
2.732ThrPhe: 2.732 ± 0.492
5.063ThrGly: 5.063 ± 0.7
1.045ThrHis: 1.045 ± 0.389
3.777ThrIle: 3.777 ± 0.589
4.42ThrLys: 4.42 ± 0.56
6.269ThrLeu: 6.269 ± 0.599
0.964ThrMet: 0.964 ± 0.248
2.893ThrAsn: 2.893 ± 0.576
2.652ThrPro: 2.652 ± 0.387
2.25ThrGln: 2.25 ± 0.522
3.375ThrArg: 3.375 ± 0.551
4.501ThrSer: 4.501 ± 0.684
4.42ThrThr: 4.42 ± 0.587
4.822ThrVal: 4.822 ± 0.609
0.563ThrTrp: 0.563 ± 0.228
2.813ThrTyr: 2.813 ± 0.614
0.0ThrXaa: 0.0 ± 0.0
Val
6.108ValAla: 6.108 ± 0.588
0.723ValCys: 0.723 ± 0.258
4.742ValAsp: 4.742 ± 0.62
4.018ValGlu: 4.018 ± 0.575
2.652ValPhe: 2.652 ± 0.541
4.259ValGly: 4.259 ± 0.578
1.205ValHis: 1.205 ± 0.264
4.018ValIle: 4.018 ± 0.598
3.858ValLys: 3.858 ± 0.505
4.983ValLeu: 4.983 ± 0.475
1.527ValMet: 1.527 ± 0.33
2.893ValAsn: 2.893 ± 0.493
1.527ValPro: 1.527 ± 0.367
4.099ValGln: 4.099 ± 0.568
2.974ValArg: 2.974 ± 0.474
4.501ValSer: 4.501 ± 0.592
3.777ValThr: 3.777 ± 0.599
4.902ValVal: 4.902 ± 0.654
0.884ValTrp: 0.884 ± 0.276
2.732ValTyr: 2.732 ± 0.38
0.0ValXaa: 0.0 ± 0.0
Trp
0.563TrpAla: 0.563 ± 0.208
0.241TrpCys: 0.241 ± 0.132
1.205TrpAsp: 1.205 ± 0.445
0.964TrpGlu: 0.964 ± 0.226
0.241TrpPhe: 0.241 ± 0.13
0.723TrpGly: 0.723 ± 0.202
0.321TrpHis: 0.321 ± 0.149
0.482TrpIle: 0.482 ± 0.174
0.723TrpLys: 0.723 ± 0.291
1.768TrpLeu: 1.768 ± 0.435
0.321TrpMet: 0.321 ± 0.186
0.964TrpAsn: 0.964 ± 0.271
0.643TrpPro: 0.643 ± 0.238
1.045TrpGln: 1.045 ± 0.302
0.884TrpArg: 0.884 ± 0.298
0.884TrpSer: 0.884 ± 0.22
0.723TrpThr: 0.723 ± 0.22
1.205TrpVal: 1.205 ± 0.301
0.0TrpTrp: 0.0 ± 0.0
0.321TrpTyr: 0.321 ± 0.129
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.893TyrAla: 2.893 ± 0.422
0.402TyrCys: 0.402 ± 0.167
2.732TyrAsp: 2.732 ± 0.324
2.009TyrGlu: 2.009 ± 0.449
1.447TyrPhe: 1.447 ± 0.364
2.411TyrGly: 2.411 ± 0.378
0.723TyrHis: 0.723 ± 0.244
2.25TyrIle: 2.25 ± 0.357
2.652TyrLys: 2.652 ± 0.344
4.179TyrLeu: 4.179 ± 0.659
1.205TyrMet: 1.205 ± 0.314
2.813TyrAsn: 2.813 ± 0.431
1.205TyrPro: 1.205 ± 0.338
1.447TyrGln: 1.447 ± 0.212
2.09TyrArg: 2.09 ± 0.273
2.974TyrSer: 2.974 ± 0.441
3.777TyrThr: 3.777 ± 0.455
2.25TyrVal: 2.25 ± 0.368
0.643TyrTrp: 0.643 ± 0.28
2.09TyrTyr: 2.09 ± 0.532
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 49 proteins (12444 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski