Streptococcus phage CHPC1091
Average proteome isoelectric point is 6.43
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 46 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3G8FB52|A0A3G8FB52_9CAUD Uncharacterized protein OS=Streptococcus phage CHPC1091 OX=2365026 GN=CHPC1091_0044 PE=4 SV=1
MM1 pKa = 7.3 SVSKK5 pKa = 9.08 EE6 pKa = 4.26 TIMQTLNLDD15 pKa = 3.8 EE16 pKa = 4.97 TDD18 pKa = 3.78 DD19 pKa = 3.8 TALIPAYY26 pKa = 9.65 IEE28 pKa = 3.9 SARR31 pKa = 11.84 QYY33 pKa = 10.3 VVNSVGDD40 pKa = 3.54 DD41 pKa = 3.37 PKK43 pKa = 10.95 FYY45 pKa = 11.07 NLDD48 pKa = 3.41 SVRR51 pKa = 11.84 ALFDD55 pKa = 3.34 TAVIALTSSYY65 pKa = 8.66 FTYY68 pKa = 10.07 RR69 pKa = 11.84 VALTDD74 pKa = 3.37 TATYY78 pKa = 9.41 PVNLTLNSIIGQLRR92 pKa = 11.84 GLYY95 pKa = 8.66 ATYY98 pKa = 10.56 SEE100 pKa = 4.46 EE101 pKa = 5.06 RR102 pKa = 11.84 GDD104 pKa = 3.53
Molecular weight: 11.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.863
IPC2_protein 4.151
IPC_protein 4.062
Toseland 3.859
ProMoST 4.139
Dawson 4.062
Bjellqvist 4.279
Wikipedia 3.999
Rodwell 3.897
Grimsley 3.77
Solomon 4.05
Lehninger 3.999
Nozaki 4.177
DTASelect 4.406
Thurlkill 3.923
EMBOSS 4.012
Sillero 4.19
Patrickios 1.964
IPC_peptide 4.037
IPC2_peptide 4.164
IPC2.peptide.svr19 4.1
Protein with the highest isoelectric point:
>tr|A0A3G8FDT5|A0A3G8FDT5_9CAUD Uncharacterized protein OS=Streptococcus phage CHPC1091 OX=2365026 GN=CHPC1091_0019 PE=4 SV=1
MM1 pKa = 7.58 ARR3 pKa = 11.84 GRR5 pKa = 11.84 YY6 pKa = 8.88 LPSDD10 pKa = 3.93 FRR12 pKa = 11.84 YY13 pKa = 9.99 KK14 pKa = 10.95 ADD16 pKa = 3.48 FGTYY20 pKa = 9.38 QSTPNKK26 pKa = 8.16 FTGVSVPKK34 pKa = 10.14 FVKK37 pKa = 10.38 QFTLHH42 pKa = 5.9 YY43 pKa = 9.16 KK44 pKa = 9.46 PHH46 pKa = 6.1 TRR48 pKa = 11.84 TLNQEE53 pKa = 3.69 YY54 pKa = 10.23 LAIQNGEE61 pKa = 3.55 NDD63 pKa = 3.11 TRR65 pKa = 11.84 VIVIRR70 pKa = 11.84 HH71 pKa = 4.8 NAKK74 pKa = 9.68 VLEE77 pKa = 4.46 GQVVTLNGTQYY88 pKa = 11.15 DD89 pKa = 3.86 IVRR92 pKa = 11.84 ISPDD96 pKa = 3.31 DD97 pKa = 3.47 NFGFNHH103 pKa = 6.65 YY104 pKa = 11.01 DD105 pKa = 3.15 FLTLRR110 pKa = 11.84 KK111 pKa = 8.91 HH112 pKa = 6.08 KK113 pKa = 10.69 KK114 pKa = 9.55 VGG116 pKa = 3.25
Molecular weight: 13.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.144
IPC2_protein 9.341
IPC_protein 9.37
Toseland 9.823
ProMoST 9.589
Dawson 10.087
Bjellqvist 9.78
Wikipedia 10.277
Rodwell 10.394
Grimsley 10.175
Solomon 10.116
Lehninger 10.072
Nozaki 9.78
DTASelect 9.78
Thurlkill 9.911
EMBOSS 10.233
Sillero 9.984
Patrickios 7.6
IPC_peptide 10.116
IPC2_peptide 8.346
IPC2.peptide.svr19 8.369
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
46
0
46
11262
45
1560
244.8
27.71
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.571 ± 0.492
0.524 ± 0.119
6.713 ± 0.248
6.517 ± 0.468
4.191 ± 0.179
6.97 ± 0.469
1.563 ± 0.116
6.66 ± 0.278
8.009 ± 0.476
7.734 ± 0.423
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.522 ± 0.18
6.304 ± 0.225
3.161 ± 0.213
4.156 ± 0.258
4.182 ± 0.268
6.065 ± 0.296
6.287 ± 0.302
6.074 ± 0.228
1.616 ± 0.148
4.182 ± 0.338
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here