Candidatus Atribacteria bacterium MT.SAG.1

Taxonomy: cellular organisms; Bacteria; Atribacterota; unclassified Atribacterota

Average proteome isoelectric point is 7.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1304 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y8I4G9|A0A4Y8I4G9_9BACT RNA-splicing ligase RtcB OS=Candidatus Atribacteria bacterium MT.SAG.1 OX=2552984 GN=rtcB PE=3 SV=1
MM1 pKa = 7.48NSMKK5 pKa = 10.26VKK7 pKa = 9.52RR8 pKa = 11.84TIIFLLVIGIMVVVAGCARR27 pKa = 11.84WPDD30 pKa = 3.76GPGPGPGEE38 pKa = 3.89PEE40 pKa = 4.0YY41 pKa = 10.79QLEE44 pKa = 4.28ITVEE48 pKa = 4.03VAGVINSSDD57 pKa = 3.51GIYY60 pKa = 10.51YY61 pKa = 9.93IVLDD65 pKa = 4.44ADD67 pKa = 4.0EE68 pKa = 4.76EE69 pKa = 4.97SIDD72 pKa = 5.02GPEE75 pKa = 4.81DD76 pKa = 4.01DD77 pKa = 5.18AKK79 pKa = 11.18DD80 pKa = 3.56WEE82 pKa = 4.51DD83 pKa = 3.18DD84 pKa = 3.8FYY86 pKa = 11.69YY87 pKa = 11.2VKK89 pKa = 10.79LDD91 pKa = 3.27GMDD94 pKa = 3.29FHH96 pKa = 7.23FARR99 pKa = 11.84VEE101 pKa = 3.95EE102 pKa = 5.39GSASMSLTDD111 pKa = 4.21SSYY114 pKa = 11.61SEE116 pKa = 5.7DD117 pKa = 3.48KK118 pKa = 11.16LQVTIALSDD127 pKa = 3.96LEE129 pKa = 5.05DD130 pKa = 4.61PNSIDD135 pKa = 3.32INVITTDD142 pKa = 3.7SDD144 pKa = 3.74NNTYY148 pKa = 11.33DD149 pKa = 3.62HH150 pKa = 7.44LDD152 pKa = 3.55GYY154 pKa = 8.65FTINTVLGEE163 pKa = 4.23TEE165 pKa = 4.01EE166 pKa = 4.79GVVSEE171 pKa = 4.78GGSEE175 pKa = 3.82EE176 pKa = 4.19GGADD180 pKa = 3.38FDD182 pKa = 4.77IIKK185 pKa = 8.57VTAEE189 pKa = 3.44ITTLYY194 pKa = 10.88

Molecular weight: 21.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y8I0J7|A0A4Y8I0J7_9BACT Uncharacterized protein OS=Candidatus Atribacteria bacterium MT.SAG.1 OX=2552984 GN=E3V08_03490 PE=4 SV=1
MM1 pKa = 7.32ARR3 pKa = 11.84YY4 pKa = 9.69GFFRR8 pKa = 11.84RR9 pKa = 11.84PQRR12 pKa = 11.84KK13 pKa = 8.12VCLFCKK19 pKa = 9.99EE20 pKa = 4.6KK21 pKa = 10.6IVADD25 pKa = 3.97YY26 pKa = 11.33KK27 pKa = 11.39DD28 pKa = 3.34VDD30 pKa = 4.32LLRR33 pKa = 11.84KK34 pKa = 9.88FITEE38 pKa = 3.57QGKK41 pKa = 9.04ILSRR45 pKa = 11.84RR46 pKa = 11.84VTGNCARR53 pKa = 11.84HH54 pKa = 5.04QRR56 pKa = 11.84TLAMSIKK63 pKa = 9.44RR64 pKa = 11.84ARR66 pKa = 11.84EE67 pKa = 3.5IGLLHH72 pKa = 5.97YY73 pKa = 9.61VAKK76 pKa = 10.67

Molecular weight: 9.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1304

0

1304

391623

25

1694

300.3

33.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.93 ± 0.078

1.08 ± 0.026

4.828 ± 0.044

7.614 ± 0.07

4.402 ± 0.055

6.789 ± 0.065

1.479 ± 0.025

10.182 ± 0.072

9.455 ± 0.074

9.788 ± 0.078

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.212 ± 0.027

4.862 ± 0.056

3.517 ± 0.036

2.721 ± 0.033

4.229 ± 0.046

5.784 ± 0.046

4.541 ± 0.041

6.252 ± 0.052

0.804 ± 0.022

3.533 ± 0.046
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski