Collichthys lucidus (Big head croaker) (Sciaena lucida)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii; Actinopteri; Neopterygii; Teleostei; Osteoglossocephalai; Clupeocephala; Euteleosteomorpha; Neoteleostei; Eurypterygia;

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 27208 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4U5TVY1|A0A4U5TVY1_COLLU Protein unc-80-like protein OS=Collichthys lucidus OX=240159 GN=D9C73_027713 PE=4 SV=1
MM1 pKa = 7.96IDD3 pKa = 4.76RR4 pKa = 11.84DD5 pKa = 4.14MIDD8 pKa = 4.84CDD10 pKa = 5.55VIDD13 pKa = 5.85CDD15 pKa = 5.73MIDD18 pKa = 4.12CDD20 pKa = 4.44VFDD23 pKa = 5.56RR24 pKa = 11.84DD25 pKa = 3.67VFDD28 pKa = 5.68RR29 pKa = 11.84DD30 pKa = 3.45VFDD33 pKa = 5.68RR34 pKa = 11.84DD35 pKa = 3.45VFDD38 pKa = 6.19RR39 pKa = 11.84DD40 pKa = 4.02VFDD43 pKa = 6.24CDD45 pKa = 5.14VFDD48 pKa = 6.49CDD50 pKa = 5.84VFDD53 pKa = 6.49CDD55 pKa = 5.84VFDD58 pKa = 5.74CDD60 pKa = 4.46VFDD63 pKa = 6.55RR64 pKa = 11.84DD65 pKa = 4.94VFDD68 pKa = 5.6CDD70 pKa = 3.53VFDD73 pKa = 6.55RR74 pKa = 11.84DD75 pKa = 4.94VFDD78 pKa = 5.6CDD80 pKa = 3.53VFDD83 pKa = 6.55RR84 pKa = 11.84DD85 pKa = 4.94VFDD88 pKa = 6.24CDD90 pKa = 5.14VFDD93 pKa = 5.74CDD95 pKa = 4.46VFDD98 pKa = 5.74RR99 pKa = 11.84DD100 pKa = 3.67VFDD103 pKa = 6.19RR104 pKa = 11.84DD105 pKa = 4.02VFDD108 pKa = 5.6CDD110 pKa = 3.53VFDD113 pKa = 6.55RR114 pKa = 11.84DD115 pKa = 4.94VFDD118 pKa = 6.24CDD120 pKa = 5.14VFDD123 pKa = 5.74CDD125 pKa = 4.46VFDD128 pKa = 6.55RR129 pKa = 11.84DD130 pKa = 4.94VFDD133 pKa = 5.6CDD135 pKa = 3.53VFDD138 pKa = 6.55RR139 pKa = 11.84DD140 pKa = 4.94VFDD143 pKa = 5.6CDD145 pKa = 3.53VFDD148 pKa = 6.55RR149 pKa = 11.84DD150 pKa = 4.94VFDD153 pKa = 5.6CDD155 pKa = 3.53VFDD158 pKa = 6.55RR159 pKa = 11.84DD160 pKa = 4.94VFDD163 pKa = 5.6CDD165 pKa = 3.53VFDD168 pKa = 5.74RR169 pKa = 11.84DD170 pKa = 3.67VFDD173 pKa = 5.68RR174 pKa = 11.84DD175 pKa = 3.45VFDD178 pKa = 5.68RR179 pKa = 11.84DD180 pKa = 3.45VFDD183 pKa = 6.19RR184 pKa = 11.84DD185 pKa = 4.02VFDD188 pKa = 6.24CDD190 pKa = 5.14VFDD193 pKa = 5.74CDD195 pKa = 4.46VFDD198 pKa = 6.55RR199 pKa = 11.84DD200 pKa = 4.94VFDD203 pKa = 6.24CDD205 pKa = 5.14VFDD208 pKa = 6.49CDD210 pKa = 5.84VFDD213 pKa = 5.74CDD215 pKa = 4.46VFDD218 pKa = 6.55RR219 pKa = 11.84DD220 pKa = 4.94VFDD223 pKa = 5.6CDD225 pKa = 3.49VFDD228 pKa = 6.49RR229 pKa = 11.84DD230 pKa = 5.38VIDD233 pKa = 5.75CDD235 pKa = 4.82VFDD238 pKa = 5.81CDD240 pKa = 5.13VIDD243 pKa = 5.91RR244 pKa = 11.84DD245 pKa = 4.62VFDD248 pKa = 5.66CDD250 pKa = 4.13VIDD253 pKa = 4.92RR254 pKa = 11.84DD255 pKa = 4.15VIDD258 pKa = 5.57RR259 pKa = 11.84DD260 pKa = 4.0VFDD263 pKa = 6.13CDD265 pKa = 4.55VFDD268 pKa = 5.74CNVIDD273 pKa = 6.05RR274 pKa = 11.84DD275 pKa = 4.14VFDD278 pKa = 5.66CDD280 pKa = 4.27VIDD283 pKa = 4.72RR284 pKa = 11.84DD285 pKa = 3.86VFDD288 pKa = 6.07RR289 pKa = 11.84DD290 pKa = 3.94VFDD293 pKa = 5.55CDD295 pKa = 4.27VIDD298 pKa = 5.91RR299 pKa = 11.84DD300 pKa = 4.79VFDD303 pKa = 6.04CDD305 pKa = 5.14VFDD308 pKa = 6.49CDD310 pKa = 5.84VFDD313 pKa = 6.49CDD315 pKa = 5.84VFDD318 pKa = 6.49CDD320 pKa = 5.84VFDD323 pKa = 6.49CDD325 pKa = 5.84VFDD328 pKa = 5.74CDD330 pKa = 4.46VFDD333 pKa = 5.74RR334 pKa = 11.84DD335 pKa = 3.67VFDD338 pKa = 6.19RR339 pKa = 11.84DD340 pKa = 4.02VFDD343 pKa = 6.24CDD345 pKa = 5.14VFDD348 pKa = 5.74CDD350 pKa = 4.26VFDD353 pKa = 5.78RR354 pKa = 11.84DD355 pKa = 4.18VIDD358 pKa = 4.79RR359 pKa = 11.84DD360 pKa = 3.69VFDD363 pKa = 5.72RR364 pKa = 11.84DD365 pKa = 3.45VFDD368 pKa = 5.68RR369 pKa = 11.84DD370 pKa = 3.45VFDD373 pKa = 5.68RR374 pKa = 11.84DD375 pKa = 3.45VFDD378 pKa = 5.68RR379 pKa = 11.84DD380 pKa = 3.45VFDD383 pKa = 5.68RR384 pKa = 11.84DD385 pKa = 3.45VFDD388 pKa = 6.19RR389 pKa = 11.84DD390 pKa = 4.02VFDD393 pKa = 6.24CDD395 pKa = 5.14VFDD398 pKa = 6.49CDD400 pKa = 5.84VFDD403 pKa = 5.6CDD405 pKa = 5.14VFDD408 pKa = 6.11FMNHH412 pKa = 6.56DD413 pKa = 4.18YY414 pKa = 10.18MNHH417 pKa = 4.38STMPMKK423 pKa = 10.3HH424 pKa = 6.36DD425 pKa = 3.4HH426 pKa = 6.93HH427 pKa = 7.38NDD429 pKa = 2.44HH430 pKa = 6.38TMAVPTTTGHH440 pKa = 4.77IHH442 pKa = 6.76GGGGNDD448 pKa = 3.9GNHH451 pKa = 6.71GGHH454 pKa = 6.76GGMAMTFYY462 pKa = 10.77FGCNNVEE469 pKa = 4.16LLFSGLLINSPGEE482 pKa = 3.92MVGACIGVFLLAVLYY497 pKa = 10.14EE498 pKa = 4.05GLKK501 pKa = 9.13MGRR504 pKa = 11.84EE505 pKa = 3.9ALLRR509 pKa = 11.84RR510 pKa = 11.84SQVNVRR516 pKa = 11.84YY517 pKa = 10.52NSMPLPGADD526 pKa = 2.86GTVLMEE532 pKa = 3.68THH534 pKa = 5.73KK535 pKa = 9.83TVGQRR540 pKa = 11.84MLSPAHH546 pKa = 6.28FLQTLLHH553 pKa = 6.87IIQVVVSYY561 pKa = 10.47FLMLIFMTYY570 pKa = 9.8NAYY573 pKa = 10.68LCIAVAAGAGMGYY586 pKa = 10.54FLFSWRR592 pKa = 11.84KK593 pKa = 8.91AVVVDD598 pKa = 3.55ITEE601 pKa = 4.18HH602 pKa = 5.39CHH604 pKa = 5.81

Molecular weight: 70.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4U5V576|A0A4U5V576_COLLU Upstream stimulatory factor 2 OS=Collichthys lucidus OX=240159 GN=D9C73_016529 PE=4 SV=1
MM1 pKa = 7.52LNATRR6 pKa = 11.84MLNATRR12 pKa = 11.84MLNATRR18 pKa = 11.84MLNATRR24 pKa = 11.84MLNGTRR30 pKa = 11.84MLNAATRR37 pKa = 11.84MLNAATRR44 pKa = 11.84MLNAATRR51 pKa = 11.84MLNATRR57 pKa = 11.84MLNATRR63 pKa = 11.84MLNATRR69 pKa = 11.84MLNATRR75 pKa = 11.84MLNATRR81 pKa = 11.84MLNATRR87 pKa = 11.84MLNATRR93 pKa = 11.84MLNATHH99 pKa = 6.6MLNAATRR106 pKa = 11.84MLNAATRR113 pKa = 11.84MLNATRR119 pKa = 11.84MLNAATRR126 pKa = 11.84MLNATRR132 pKa = 11.84MLNAATRR139 pKa = 11.84MLNATRR145 pKa = 11.84MLNAATRR152 pKa = 11.84MLNATRR158 pKa = 11.84MLNAATRR165 pKa = 11.84MLNATRR171 pKa = 11.84MLNAATRR178 pKa = 11.84MLNATRR184 pKa = 11.84MLNARR189 pKa = 11.84VCC191 pKa = 3.72

Molecular weight: 21.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

27208

0

27208

15347301

49

38162

564.1

62.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.629 ± 0.014

2.195 ± 0.011

5.257 ± 0.011

6.964 ± 0.024

3.439 ± 0.01

6.322 ± 0.021

2.679 ± 0.009

4.217 ± 0.013

5.553 ± 0.02

9.421 ± 0.023

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.387 ± 0.008

3.771 ± 0.011

5.796 ± 0.021

4.813 ± 0.015

5.812 ± 0.015

8.786 ± 0.021

5.81 ± 0.019

6.354 ± 0.014

1.127 ± 0.006

2.667 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski