Amino acid dipepetide frequency for Prochlorococcus marinus (strain MIT 9211)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.412AlaAla: 6.412 ± 0.362	1.044AlaCys: 1.044 ± 0.052	3.248AlaAsp: 3.248 ± 0.097	4.081AlaGlu: 4.081 ± 0.095	2.921AlaPhe: 2.921 ± 0.1	5.016AlaGly: 5.016 ± 0.125	1.117AlaHis: 1.117 ± 0.048	5.929AlaIle: 5.929 ± 0.118	4.388AlaLys: 4.388 ± 0.1	8.721AlaLeu: 8.721 ± 0.143	1.802AlaMet: 1.802 ± 0.061	2.881AlaAsn: 2.881 ± 0.072	2.271AlaPro: 2.271 ± 0.076	2.457AlaGln: 2.457 ± 0.074	3.483AlaArg: 3.483 ± 0.095	5.279AlaSer: 5.279 ± 0.102	3.293AlaThr: 3.293 ± 0.078	4.513AlaVal: 4.513 ± 0.104	0.921AlaTrp: 0.921 ± 0.048	1.828AlaTyr: 1.828 ± 0.057	0.0AlaXaa: 0.0 ± 0.0
Cys	0.651CysAla: 0.651 ± 0.031	0.206CysCys: 0.206 ± 0.022	0.608CysAsp: 0.608 ± 0.033	0.683CysGlu: 0.683 ± 0.037	0.588CysPhe: 0.588 ± 0.036	1.02CysGly: 1.02 ± 0.043	0.255CysHis: 0.255 ± 0.022	0.885CysIle: 0.885 ± 0.039	0.768CysLys: 0.768 ± 0.041	1.465CysLeu: 1.465 ± 0.064	0.19CysMet: 0.19 ± 0.021	0.606CysAsn: 0.606 ± 0.036	0.594CysPro: 0.594 ± 0.029	0.358CysGln: 0.358 ± 0.029	0.622CysArg: 0.622 ± 0.041	0.943CysSer: 0.943 ± 0.049	0.56CysThr: 0.56 ± 0.036	0.6CysVal: 0.6 ± 0.034	0.228CysTrp: 0.228 ± 0.02	0.309CysTyr: 0.309 ± 0.025	0.0CysXaa: 0.0 ± 0.0
Asp	2.988AspAla: 2.988 ± 0.089	0.584AspCys: 0.584 ± 0.033	2.15AspAsp: 2.15 ± 0.075	3.087AspGlu: 3.087 ± 0.096	2.248AspPhe: 2.248 ± 0.074	3.263AspGly: 3.263 ± 0.09	0.992AspHis: 0.992 ± 0.052	3.384AspIle: 3.384 ± 0.081	2.804AspLys: 2.804 ± 0.098	7.077AspLeu: 7.077 ± 0.116	0.956AspMet: 0.956 ± 0.041	1.99AspAsn: 1.99 ± 0.075	2.824AspPro: 2.824 ± 0.074	2.232AspGln: 2.232 ± 0.063	2.586AspArg: 2.586 ± 0.082	3.588AspSer: 3.588 ± 0.098	1.824AspThr: 1.824 ± 0.061	2.721AspVal: 2.721 ± 0.078	0.887AspTrp: 0.887 ± 0.038	1.471AspTyr: 1.471 ± 0.062	0.0AspXaa: 0.0 ± 0.0
Glu	5.04GluAla: 5.04 ± 0.105	0.61GluCys: 0.61 ± 0.036	3.115GluAsp: 3.115 ± 0.077	4.721GluGlu: 4.721 ± 0.114	2.026GluPhe: 2.026 ± 0.074	4.22GluGly: 4.22 ± 0.099	1.022GluHis: 1.022 ± 0.049	5.17GluIle: 5.17 ± 0.114	4.602GluLys: 4.602 ± 0.11	7.75GluLeu: 7.75 ± 0.14	1.382GluMet: 1.382 ± 0.061	2.853GluAsn: 2.853 ± 0.087	2.172GluPro: 2.172 ± 0.071	2.218GluGln: 2.218 ± 0.067	3.554GluArg: 3.554 ± 0.092	4.267GluSer: 4.267 ± 0.11	2.919GluThr: 2.919 ± 0.083	4.194GluVal: 4.194 ± 0.104	0.927GluTrp: 0.927 ± 0.044	1.293GluTyr: 1.293 ± 0.047	0.0GluXaa: 0.0 ± 0.0
Phe	2.749PheAla: 2.749 ± 0.076	0.6PheCys: 0.6 ± 0.035	2.432PheAsp: 2.432 ± 0.072	2.267PheGlu: 2.267 ± 0.068	1.887PhePhe: 1.887 ± 0.077	3.065PheGly: 3.065 ± 0.083	0.741PheHis: 0.741 ± 0.041	2.725PheIle: 2.725 ± 0.073	2.21PheLys: 2.21 ± 0.067	4.479PheLeu: 4.479 ± 0.141	0.679PheMet: 0.679 ± 0.041	2.002PheAsn: 2.002 ± 0.077	1.616PhePro: 1.616 ± 0.062	1.459PheGln: 1.459 ± 0.05	1.899PheArg: 1.899 ± 0.063	3.392PheSer: 3.392 ± 0.097	1.881PheThr: 1.881 ± 0.058	2.129PheVal: 2.129 ± 0.071	0.642PheTrp: 0.642 ± 0.035	1.018PheTyr: 1.018 ± 0.049	0.0PheXaa: 0.0 ± 0.0
Gly	4.871GlyAla: 4.871 ± 0.123	1.05GlyCys: 1.05 ± 0.046	3.354GlyAsp: 3.354 ± 0.088	3.964GlyGlu: 3.964 ± 0.092	3.354GlyPhe: 3.354 ± 0.086	5.35GlyGly: 5.35 ± 0.17	1.426GlyHis: 1.426 ± 0.065	5.869GlyIle: 5.869 ± 0.12	4.095GlyLys: 4.095 ± 0.099	8.174GlyLeu: 8.174 ± 0.13	1.675GlyMet: 1.675 ± 0.059	2.774GlyAsn: 2.774 ± 0.072	2.455GlyPro: 2.455 ± 0.079	2.347GlyGln: 2.347 ± 0.076	3.467GlyArg: 3.467 ± 0.088	4.954GlySer: 4.954 ± 0.107	3.408GlyThr: 3.408 ± 0.081	4.665GlyVal: 4.665 ± 0.1	1.246GlyTrp: 1.246 ± 0.058	2.028GlyTyr: 2.028 ± 0.061	0.0GlyXaa: 0.0 ± 0.0
His	1.147HisAla: 1.147 ± 0.053	0.297HisCys: 0.297 ± 0.024	0.721HisAsp: 0.721 ± 0.046	0.941HisGlu: 0.941 ± 0.046	0.762HisPhe: 0.762 ± 0.046	1.436HisGly: 1.436 ± 0.061	0.588HisHis: 0.588 ± 0.042	1.164HisIle: 1.164 ± 0.051	0.962HisLys: 0.962 ± 0.05	2.362HisLeu: 2.362 ± 0.082	0.283HisMet: 0.283 ± 0.026	0.76HisAsn: 0.76 ± 0.039	1.16HisPro: 1.16 ± 0.043	0.867HisGln: 0.867 ± 0.041	1.085HisArg: 1.085 ± 0.044	1.414HisSer: 1.414 ± 0.058	0.764HisThr: 0.764 ± 0.041	0.828HisVal: 0.828 ± 0.037	0.376HisTrp: 0.376 ± 0.03	0.511HisTyr: 0.511 ± 0.031	0.0HisXaa: 0.0 ± 0.0
Ile	6.046IleAla: 6.046 ± 0.11	1.075IleCys: 1.075 ± 0.051	4.653IleAsp: 4.653 ± 0.101	5.117IleGlu: 5.117 ± 0.115	2.808IlePhe: 2.808 ± 0.089	5.729IleGly: 5.729 ± 0.112	1.408IleHis: 1.408 ± 0.054	4.521IleIle: 4.521 ± 0.099	4.547IleLys: 4.547 ± 0.114	6.875IleLeu: 6.875 ± 0.134	0.988IleMet: 0.988 ± 0.045	4.246IleAsn: 4.246 ± 0.091	3.689IlePro: 3.689 ± 0.081	2.519IleGln: 2.519 ± 0.069	3.535IleArg: 3.535 ± 0.096	6.218IleSer: 6.218 ± 0.123	3.846IleThr: 3.846 ± 0.094	4.0IleVal: 4.0 ± 0.096	0.925IleTrp: 0.925 ± 0.041	1.754IleTyr: 1.754 ± 0.049	0.0IleXaa: 0.0 ± 0.0
Lys	4.814LysAla: 4.814 ± 0.118	0.547LysCys: 0.547 ± 0.032	3.6LysAsp: 3.6 ± 0.098	5.184LysGlu: 5.184 ± 0.124	1.85LysPhe: 1.85 ± 0.064	4.248LysGly: 4.248 ± 0.082	0.889LysHis: 0.889 ± 0.045	3.958LysIle: 3.958 ± 0.107	4.63LysLys: 4.63 ± 0.132	6.602LysLeu: 6.602 ± 0.135	1.113LysMet: 1.113 ± 0.042	3.283LysAsn: 3.283 ± 0.08	2.644LysPro: 2.644 ± 0.07	2.343LysGln: 2.343 ± 0.079	3.4LysArg: 3.4 ± 0.075	4.737LysSer: 4.737 ± 0.106	3.061LysThr: 3.061 ± 0.078	3.83LysVal: 3.83 ± 0.084	0.766LysTrp: 0.766 ± 0.04	1.554LysTyr: 1.554 ± 0.062	0.0LysXaa: 0.0 ± 0.0
Leu	8.857LeuAla: 8.857 ± 0.151	1.325LeuCys: 1.325 ± 0.057	5.778LeuAsp: 5.778 ± 0.115	8.103LeuGlu: 8.103 ± 0.152	4.147LeuPhe: 4.147 ± 0.097	8.232LeuGly: 8.232 ± 0.154	1.98LeuHis: 1.98 ± 0.073	9.455LeuIle: 9.455 ± 0.167	7.44LeuLys: 7.44 ± 0.125	13.275LeuLeu: 13.275 ± 0.218	2.372LeuMet: 2.372 ± 0.07	5.196LeuAsn: 5.196 ± 0.091	5.467LeuPro: 5.467 ± 0.097	4.099LeuGln: 4.099 ± 0.096	5.901LeuArg: 5.901 ± 0.118	8.919LeuSer: 8.919 ± 0.137	5.541LeuThr: 5.541 ± 0.108	7.024LeuVal: 7.024 ± 0.125	1.444LeuTrp: 1.444 ± 0.059	2.176LeuTyr: 2.176 ± 0.073	0.0LeuXaa: 0.0 ± 0.0
Met	1.952MetAla: 1.952 ± 0.071	0.121MetCys: 0.121 ± 0.015	0.861MetAsp: 0.861 ± 0.047	1.194MetGlu: 1.194 ± 0.045	0.58MetPhe: 0.58 ± 0.036	1.545MetGly: 1.545 ± 0.059	0.352MetHis: 0.352 ± 0.027	1.257MetIle: 1.257 ± 0.052	1.289MetLys: 1.289 ± 0.051	1.895MetLeu: 1.895 ± 0.062	0.321MetMet: 0.321 ± 0.028	1.063MetAsn: 1.063 ± 0.044	1.145MetPro: 1.145 ± 0.057	0.796MetGln: 0.796 ± 0.043	1.02MetArg: 1.02 ± 0.041	1.531MetSer: 1.531 ± 0.051	1.271MetThr: 1.271 ± 0.048	1.21MetVal: 1.21 ± 0.049	0.103MetTrp: 0.103 ± 0.017	0.273MetTyr: 0.273 ± 0.02	0.0MetXaa: 0.0 ± 0.0
Asn	2.927AsnAla: 2.927 ± 0.074	0.653AsnCys: 0.653 ± 0.04	2.23AsnAsp: 2.23 ± 0.072	2.911AsnGlu: 2.911 ± 0.076	1.792AsnPhe: 1.792 ± 0.066	2.81AsnGly: 2.81 ± 0.067	0.941AsnHis: 0.941 ± 0.039	3.186AsnIle: 3.186 ± 0.085	3.073AsnLys: 3.073 ± 0.084	5.25AsnLeu: 5.25 ± 0.102	0.873AsnMet: 0.873 ± 0.042	2.572AsnAsn: 2.572 ± 0.096	2.525AsnPro: 2.525 ± 0.084	2.388AsnGln: 2.388 ± 0.07	2.347AsnArg: 2.347 ± 0.075	3.982AsnSer: 3.982 ± 0.093	2.081AsnThr: 2.081 ± 0.07	2.129AsnVal: 2.129 ± 0.068	0.826AsnTrp: 0.826 ± 0.042	1.343AsnTyr: 1.343 ± 0.059	0.0AsnXaa: 0.0 ± 0.0
Pro	2.457ProAla: 2.457 ± 0.068	0.444ProCys: 0.444 ± 0.028	2.216ProAsp: 2.216 ± 0.059	3.127ProGlu: 3.127 ± 0.083	1.962ProPhe: 1.962 ± 0.074	3.137ProGly: 3.137 ± 0.081	0.818ProHis: 0.818 ± 0.045	3.495ProIle: 3.495 ± 0.073	2.758ProLys: 2.758 ± 0.074	5.117ProLeu: 5.117 ± 0.104	0.79ProMet: 0.79 ± 0.037	2.113ProAsn: 2.113 ± 0.06	1.671ProPro: 1.671 ± 0.063	1.461ProGln: 1.461 ± 0.053	1.855ProArg: 1.855 ± 0.064	3.556ProSer: 3.556 ± 0.092	2.133ProThr: 2.133 ± 0.065	2.701ProVal: 2.701 ± 0.074	0.713ProTrp: 0.713 ± 0.038	1.2ProTyr: 1.2 ± 0.055	0.0ProXaa: 0.0 ± 0.0
Gln	2.768GlnAla: 2.768 ± 0.072	0.378GlnCys: 0.378 ± 0.029	1.483GlnAsp: 1.483 ± 0.05	2.786GlnGlu: 2.786 ± 0.079	1.297GlnPhe: 1.297 ± 0.056	2.386GlnGly: 2.386 ± 0.066	0.588GlnHis: 0.588 ± 0.036	2.784GlnIle: 2.784 ± 0.07	2.543GlnLys: 2.543 ± 0.071	4.743GlnLeu: 4.743 ± 0.105	0.788GlnMet: 0.788 ± 0.039	1.58GlnAsn: 1.58 ± 0.053	1.477GlnPro: 1.477 ± 0.054	1.465GlnGln: 1.465 ± 0.057	2.107GlnArg: 2.107 ± 0.068	2.77GlnSer: 2.77 ± 0.077	1.6GlnThr: 1.6 ± 0.058	2.269GlnVal: 2.269 ± 0.067	0.612GlnTrp: 0.612 ± 0.034	0.727GlnTyr: 0.727 ± 0.043	0.0GlnXaa: 0.0 ± 0.0
Arg	2.992ArgAla: 2.992 ± 0.073	0.541ArgCys: 0.541 ± 0.033	2.378ArgAsp: 2.378 ± 0.068	3.255ArgGlu: 3.255 ± 0.1	2.325ArgPhe: 2.325 ± 0.061	2.891ArgGly: 2.891 ± 0.086	1.014ArgHis: 1.014 ± 0.046	3.834ArgIle: 3.834 ± 0.09	3.299ArgLys: 3.299 ± 0.093	6.475ArgLeu: 6.475 ± 0.129	1.053ArgMet: 1.053 ± 0.044	2.384ArgAsn: 2.384 ± 0.065	2.073ArgPro: 2.073 ± 0.062	2.046ArgGln: 2.046 ± 0.075	3.386ArgArg: 3.386 ± 0.109	3.689ArgSer: 3.689 ± 0.09	2.083ArgThr: 2.083 ± 0.066	3.002ArgVal: 3.002 ± 0.092	0.889ArgTrp: 0.889 ± 0.043	1.44ArgTyr: 1.44 ± 0.055	0.0ArgXaa: 0.0 ± 0.0
Ser	4.539SerAla: 4.539 ± 0.118	0.923SerCys: 0.923 ± 0.04	3.665SerAsp: 3.665 ± 0.098	4.242SerGlu: 4.242 ± 0.1	3.354SerPhe: 3.354 ± 0.097	5.188SerGly: 5.188 ± 0.106	1.509SerHis: 1.509 ± 0.051	6.113SerIle: 6.113 ± 0.134	5.093SerLys: 5.093 ± 0.105	9.259SerLeu: 9.259 ± 0.136	1.657SerMet: 1.657 ± 0.056	3.962SerAsn: 3.962 ± 0.083	3.216SerPro: 3.216 ± 0.083	3.032SerGln: 3.032 ± 0.077	3.774SerArg: 3.774 ± 0.083	6.624SerSer: 6.624 ± 0.148	3.897SerThr: 3.897 ± 0.1	3.62SerVal: 3.62 ± 0.097	1.23SerTrp: 1.23 ± 0.053	1.913SerTyr: 1.913 ± 0.071	0.0SerXaa: 0.0 ± 0.0
Thr	3.392ThrAla: 3.392 ± 0.087	0.574ThrCys: 0.574 ± 0.03	2.19ThrAsp: 2.19 ± 0.064	2.487ThrGlu: 2.487 ± 0.088	1.933ThrPhe: 1.933 ± 0.06	3.77ThrGly: 3.77 ± 0.105	0.81ThrHis: 0.81 ± 0.042	3.42ThrIle: 3.42 ± 0.084	2.84ThrLys: 2.84 ± 0.074	5.164ThrLeu: 5.164 ± 0.12	0.871ThrMet: 0.871 ± 0.041	2.394ThrAsn: 2.394 ± 0.074	2.475ThrPro: 2.475 ± 0.062	1.505ThrGln: 1.505 ± 0.055	2.097ThrArg: 2.097 ± 0.065	3.947ThrSer: 3.947 ± 0.086	2.539ThrThr: 2.539 ± 0.083	2.669ThrVal: 2.669 ± 0.081	0.582ThrTrp: 0.582 ± 0.036	1.194ThrTyr: 1.194 ± 0.052	0.0ThrXaa: 0.0 ± 0.0
Val	4.547ValAla: 4.547 ± 0.101	0.602ValCys: 0.602 ± 0.036	3.269ValAsp: 3.269 ± 0.09	3.606ValGlu: 3.606 ± 0.085	2.38ValPhe: 2.38 ± 0.071	4.162ValGly: 4.162 ± 0.098	1.154ValHis: 1.154 ± 0.045	4.689ValIle: 4.689 ± 0.104	3.279ValLys: 3.279 ± 0.085	6.972ValLeu: 6.972 ± 0.128	1.192ValMet: 1.192 ± 0.048	2.677ValAsn: 2.677 ± 0.068	2.446ValPro: 2.446 ± 0.064	1.893ValGln: 1.893 ± 0.058	2.75ValArg: 2.75 ± 0.086	4.038ValSer: 4.038 ± 0.089	2.651ValThr: 2.651 ± 0.086	4.261ValVal: 4.261 ± 0.107	0.665ValTrp: 0.665 ± 0.042	1.152ValTyr: 1.152 ± 0.051	0.0ValXaa: 0.0 ± 0.0
Trp	0.943TrpAla: 0.943 ± 0.048	0.208TrpCys: 0.208 ± 0.02	0.697TrpAsp: 0.697 ± 0.041	0.877TrpGlu: 0.877 ± 0.042	0.6TrpPhe: 0.6 ± 0.039	0.943TrpGly: 0.943 ± 0.047	0.406TrpHis: 0.406 ± 0.03	1.103TrpIle: 1.103 ± 0.052	0.81TrpLys: 0.81 ± 0.044	2.069TrpLeu: 2.069 ± 0.074	0.368TrpMet: 0.368 ± 0.03	0.624TrpAsn: 0.624 ± 0.037	0.642TrpPro: 0.642 ± 0.038	0.669TrpGln: 0.669 ± 0.04	0.772TrpArg: 0.772 ± 0.042	1.042TrpSer: 1.042 ± 0.043	0.558TrpThr: 0.558 ± 0.031	0.814TrpVal: 0.814 ± 0.04	0.281TrpTrp: 0.281 ± 0.025	0.299TrpTyr: 0.299 ± 0.023	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.513TyrAla: 1.513 ± 0.061	0.36TyrCys: 0.36 ± 0.028	1.008TyrAsp: 1.008 ± 0.049	1.422TyrGlu: 1.422 ± 0.057	1.057TyrPhe: 1.057 ± 0.055	2.119TyrGly: 2.119 ± 0.075	0.42TyrHis: 0.42 ± 0.026	1.554TyrIle: 1.554 ± 0.052	1.622TyrLys: 1.622 ± 0.057	3.004TyrLeu: 3.004 ± 0.087	0.422TyrMet: 0.422 ± 0.027	0.875TyrAsn: 0.875 ± 0.041	1.162TyrPro: 1.162 ± 0.044	0.998TyrGln: 0.998 ± 0.045	1.408TyrArg: 1.408 ± 0.049	1.923TyrSer: 1.923 ± 0.064	0.943TyrThr: 0.943 ± 0.052	1.21TyrVal: 1.21 ± 0.05	0.463TyrTrp: 0.463 ± 0.029	0.63TyrTyr: 0.63 ± 0.042	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 1855 proteins (505001 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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