Cyanophage P-RSM6

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Libanvirus; unclassified Libanvirus

Average proteome isoelectric point is 5.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 221 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M4SLE5|M4SLE5_9CAUD Uncharacterized protein OS=Cyanophage P-RSM6 OX=929832 GN=CPXG_00010 PE=4 SV=1
MM1 pKa = 7.54DD2 pKa = 5.0QIAFYY7 pKa = 10.94YY8 pKa = 10.62LAFFLTIGSFLLGFVVSWNLKK29 pKa = 9.64HH30 pKa = 7.24VFDD33 pKa = 3.85TWEE36 pKa = 3.9EE37 pKa = 3.95KK38 pKa = 10.62ADD40 pKa = 3.63YY41 pKa = 10.63AEE43 pKa = 4.79QILHH47 pKa = 6.8PEE49 pKa = 4.25MYY51 pKa = 10.32DD52 pKa = 3.53ADD54 pKa = 4.39GEE56 pKa = 4.4PLASPDD62 pKa = 3.56EE63 pKa = 4.0ILYY66 pKa = 10.71LRR68 pKa = 11.84FTDD71 pKa = 4.28VSDD74 pKa = 4.37TIDD77 pKa = 4.17DD78 pKa = 4.1EE79 pKa = 4.6EE80 pKa = 4.54DD81 pKa = 2.99

Molecular weight: 9.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M4T2C3|M4T2C3_9CAUD Uncharacterized protein OS=Cyanophage P-RSM6 OX=929832 GN=CPXG_00181 PE=4 SV=1
MM1 pKa = 7.53ISSLILITLGKK12 pKa = 9.44QVSIGQVEE20 pKa = 4.38QVFKK24 pKa = 11.57SNLSVNRR31 pKa = 11.84FVFSRR36 pKa = 11.84HH37 pKa = 5.64LLLSTLTTPTTPRR50 pKa = 11.84KK51 pKa = 9.56RR52 pKa = 11.84RR53 pKa = 11.84TRR55 pKa = 11.84KK56 pKa = 5.58TTPAKK61 pKa = 10.36PKK63 pKa = 9.31VDD65 pKa = 4.1VIKK68 pKa = 11.04VEE70 pKa = 4.13PTKK73 pKa = 10.64TEE75 pKa = 3.83PDD77 pKa = 3.48FSKK80 pKa = 11.0LSGIDD85 pKa = 4.02FVILPLIYY93 pKa = 10.78LEE95 pKa = 4.23AFVVNFLMNCGLKK108 pKa = 10.51VPDD111 pKa = 3.7RR112 pKa = 11.84VAVKK116 pKa = 10.43

Molecular weight: 13.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

221

0

221

61893

50

8573

280.1

31.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.577 ± 0.22

0.966 ± 0.072

6.867 ± 0.168

6.216 ± 0.32

4.199 ± 0.113

7.7 ± 0.295

1.86 ± 0.102

6.316 ± 0.205

6.041 ± 0.467

7.031 ± 0.204

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.097 ± 0.191

6.017 ± 0.221

4.157 ± 0.164

3.703 ± 0.078

4.015 ± 0.192

6.804 ± 0.183

7.469 ± 0.52

6.39 ± 0.183

1.309 ± 0.084

4.265 ± 0.136
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski