Rhizobium sp. NXC24

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium; unclassified Rhizobium

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6922 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2L0W7N5|A0A2L0W7N5_9RHIZ DnaJ family heat shock protein OS=Rhizobium sp. NXC24 OX=2048897 GN=NXC24_CH00910 PE=4 SV=1
MM1 pKa = 7.61SIFGSMKK8 pKa = 9.3TAVSGMNAQANRR20 pKa = 11.84LGTVSDD26 pKa = 4.7NIANSSTTGYY36 pKa = 10.54KK37 pKa = 9.74EE38 pKa = 3.79ASTSFSSLVLPSSAGSYY55 pKa = 10.52SSGGVQTSVHH65 pKa = 6.07RR66 pKa = 11.84AISQQGNISYY76 pKa = 7.58TTSSTDD82 pKa = 4.23LAIQGNGFFVVQSSSGQTFLSRR104 pKa = 11.84SGDD107 pKa = 3.6FSADD111 pKa = 3.06SSGNLVNSAGFTLMGYY127 pKa = 9.34PYY129 pKa = 9.95SSSGTASVVVNGFSGLEE146 pKa = 4.07PINVSEE152 pKa = 4.35NGVTAVATTTGEE164 pKa = 4.07MEE166 pKa = 4.56GNLDD170 pKa = 3.87SNADD174 pKa = 3.33IATSSTTGYY183 pKa = 10.39LPSEE187 pKa = 3.94NTYY190 pKa = 10.73PVTDD194 pKa = 3.83DD195 pKa = 3.61TNKK198 pKa = 9.88VSITGYY204 pKa = 10.42DD205 pKa = 3.43SQGNATTFDD214 pKa = 3.22VYY216 pKa = 9.39YY217 pKa = 10.49TKK219 pKa = 10.27TADD222 pKa = 3.58NTWDD226 pKa = 3.33VTVYY230 pKa = 10.7NQADD234 pKa = 4.27LVSDD238 pKa = 4.02PTSGGALYY246 pKa = 10.85SSDD249 pKa = 3.39PVGSTEE255 pKa = 6.01LDD257 pKa = 3.52FDD259 pKa = 4.0DD260 pKa = 4.98TGALTSGGTFDD271 pKa = 5.86LSFTSGSTTQTIAMDD286 pKa = 3.58MTGFTQVATDD296 pKa = 4.09FNATGSMNGQAPNPVTSVTISKK318 pKa = 10.45DD319 pKa = 3.2GTVNAVYY326 pKa = 10.44KK327 pKa = 10.76DD328 pKa = 3.83SSTKK332 pKa = 10.04ALYY335 pKa = 9.9RR336 pKa = 11.84IPLATVASPDD346 pKa = 3.51NLTLEE351 pKa = 4.42SGNVYY356 pKa = 10.21SANGEE361 pKa = 4.22SGVTVTGFPQTGGFGYY377 pKa = 9.57IQSGALEE384 pKa = 4.23EE385 pKa = 4.93SNVDD389 pKa = 4.04LATEE393 pKa = 4.52LTNMIEE399 pKa = 4.12AQKK402 pKa = 10.97SYY404 pKa = 9.42TANSKK409 pKa = 9.73VFQAGSDD416 pKa = 3.75LLDD419 pKa = 3.26VLVNLQRR426 pKa = 4.57

Molecular weight: 43.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2L0W6L9|A0A2L0W6L9_9RHIZ Uncharacterized protein OS=Rhizobium sp. NXC24 OX=2048897 GN=NXC24_CH00539 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.11GGRR28 pKa = 11.84AVLSARR34 pKa = 11.84RR35 pKa = 11.84NRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.03RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6922

0

6922

2111068

29

5174

305.0

33.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.608 ± 0.035

0.854 ± 0.01

5.721 ± 0.026

5.654 ± 0.029

3.944 ± 0.02

8.144 ± 0.033

2.095 ± 0.013

5.837 ± 0.022

3.817 ± 0.026

9.914 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.517 ± 0.017

3.002 ± 0.017

4.864 ± 0.019

3.232 ± 0.021

6.592 ± 0.031

5.963 ± 0.025

5.379 ± 0.03

7.164 ± 0.022

1.319 ± 0.013

2.379 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski