Enterobacteria phage N4 (Bacteriophage N4)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Schitoviridae; Enquatrovirinae; Enquatrovirus

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 72 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0MZA9|A0MZA9_BPN4 Gp19 OS=Enterobacteria phage N4 OX=10752 PE=4 SV=1
MM1 pKa = 7.5ANKK4 pKa = 9.6NRR6 pKa = 11.84LRR8 pKa = 11.84VWHH11 pKa = 6.79IPQVPGKK18 pKa = 9.58AFYY21 pKa = 11.29VEE23 pKa = 3.89VDD25 pKa = 3.55SVEE28 pKa = 3.97EE29 pKa = 4.04GVRR32 pKa = 11.84IIDD35 pKa = 3.24ILANYY40 pKa = 10.59DD41 pKa = 3.41LFQYY45 pKa = 10.19EE46 pKa = 4.25NNIKK50 pKa = 10.58GDD52 pKa = 3.66YY53 pKa = 10.15CNVSGLQMYY62 pKa = 9.93DD63 pKa = 3.06EE64 pKa = 5.96HH65 pKa = 6.79ITDD68 pKa = 4.98DD69 pKa = 3.7EE70 pKa = 4.33MEE72 pKa = 4.31EE73 pKa = 3.95MGLTDD78 pKa = 5.19RR79 pKa = 11.84WVDD82 pKa = 2.88WYY84 pKa = 11.67YY85 pKa = 11.35EE86 pKa = 4.07DD87 pKa = 5.36DD88 pKa = 4.63QNFFDD93 pKa = 5.39NPKK96 pKa = 10.5EE97 pKa = 3.87YY98 pKa = 11.17LNNLKK103 pKa = 10.38KK104 pKa = 10.79

Molecular weight: 12.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0MZB3|A0MZB3_BPN4 Gp23 OS=Enterobacteria phage N4 OX=10752 PE=4 SV=1
MM1 pKa = 7.18AQEE4 pKa = 3.98KK5 pKa = 10.18NRR7 pKa = 11.84RR8 pKa = 11.84QTLIEE13 pKa = 4.82RR14 pKa = 11.84IQARR18 pKa = 11.84IKK20 pKa = 10.87LQDD23 pKa = 3.26LGYY26 pKa = 8.55TVNGQPSLCHH36 pKa = 6.0IWTGSHH42 pKa = 6.17SGNGRR47 pKa = 11.84GGQYY51 pKa = 11.06GRR53 pKa = 11.84ISVNGVTSATHH64 pKa = 6.33IIAYY68 pKa = 5.27THH70 pKa = 4.68YY71 pKa = 10.48HH72 pKa = 6.22GYY74 pKa = 9.71IPNKK78 pKa = 8.42MQVDD82 pKa = 3.97HH83 pKa = 6.93LCNNRR88 pKa = 11.84LCCNPEE94 pKa = 3.64HH95 pKa = 6.89LEE97 pKa = 4.37LVTHH101 pKa = 6.2QEE103 pKa = 3.88NQRR106 pKa = 11.84RR107 pKa = 11.84RR108 pKa = 11.84AKK110 pKa = 10.22RR111 pKa = 11.84SKK113 pKa = 10.95SKK115 pKa = 10.9GNKK118 pKa = 8.6

Molecular weight: 13.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

72

0

72

22029

42

3500

306.0

34.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.475 ± 0.545

0.922 ± 0.173

5.824 ± 0.154

6.532 ± 0.256

3.545 ± 0.187

6.401 ± 0.229

1.879 ± 0.165

5.983 ± 0.194

6.7 ± 0.279

8.239 ± 0.283

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.978 ± 0.1

5.588 ± 0.18

4.104 ± 0.186

4.372 ± 0.293

4.312 ± 0.179

5.774 ± 0.237

6.573 ± 0.247

6.818 ± 0.216

1.316 ± 0.194

3.663 ± 0.248
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski