Phocoena spinipinnis papillomavirus (isolate Burmeister s porpoise/Peru/PsPV1) (PsPV)
Average proteome isoelectric point is 6.57
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q8UZ14|Q8UZ14_PSPVP E5b protein (Fragment) OS=Phocoena spinipinnis papillomavirus (isolate Burmeister's porpoise/Peru/PsPV1) OX=654916 GN=e5b PE=4 SV=1
MM1 pKa = 6.98 VRR3 pKa = 11.84 VKK5 pKa = 10.36 RR6 pKa = 11.84 RR7 pKa = 11.84 RR8 pKa = 11.84 RR9 pKa = 11.84 AAEE12 pKa = 3.75 GDD14 pKa = 3.74 LYY16 pKa = 11.14 AGCRR20 pKa = 11.84 RR21 pKa = 11.84 GQDD24 pKa = 3.42 CPDD27 pKa = 4.52 DD28 pKa = 3.73 IKK30 pKa = 11.33 PKK32 pKa = 10.54 FEE34 pKa = 3.81 QDD36 pKa = 2.38 TWADD40 pKa = 3.89 RR41 pKa = 11.84 FLKK44 pKa = 9.65 WFSSIIYY51 pKa = 9.6 LGNLGISTGRR61 pKa = 11.84 GAGGSTGYY69 pKa = 10.76 VPVGSGGGRR78 pKa = 11.84 GVRR81 pKa = 11.84 PAMGGQPSRR90 pKa = 11.84 PNVVVEE96 pKa = 4.1 NVGPAEE102 pKa = 4.18 VPVDD106 pKa = 3.75 GAVDD110 pKa = 3.9 ASAPSVITPSEE121 pKa = 4.16 STVVVGGSTTPHH133 pKa = 6.33 EE134 pKa = 5.15 EE135 pKa = 3.85 IPLVPLHH142 pKa = 6.78 PEE144 pKa = 3.81 VGPDD148 pKa = 3.84 PEE150 pKa = 6.17 PGLPLPPPEE159 pKa = 5.21 SGGPAVLDD167 pKa = 3.24 VTLNVTSTYY176 pKa = 9.5 THH178 pKa = 7.39 DD179 pKa = 3.91 PSIIHH184 pKa = 6.36 PRR186 pKa = 11.84 VSSLGEE192 pKa = 3.9 SAGAEE197 pKa = 3.69 APLFPTISLQPLDD210 pKa = 4.04 VSLLPGEE217 pKa = 5.33 SSFQPHH223 pKa = 6.97 AYY225 pKa = 8.98 IDD227 pKa = 3.73 LSGSFEE233 pKa = 4.45 EE234 pKa = 4.75 IEE236 pKa = 4.23 LDD238 pKa = 3.55 AFTSGPQDD246 pKa = 3.72 PQTSTPMSRR255 pKa = 11.84 VDD257 pKa = 3.0 SGLRR261 pKa = 11.84 SVRR264 pKa = 11.84 RR265 pKa = 11.84 AYY267 pKa = 10.47 SRR269 pKa = 11.84 RR270 pKa = 11.84 TGALRR275 pKa = 11.84 RR276 pKa = 11.84 LYY278 pKa = 10.46 HH279 pKa = 7.34 RR280 pKa = 11.84 LTQQVRR286 pKa = 11.84 VNRR289 pKa = 11.84 PEE291 pKa = 3.7 FLRR294 pKa = 11.84 RR295 pKa = 11.84 PSQLVSYY302 pKa = 10.5 VFDD305 pKa = 3.83 NAAFDD310 pKa = 4.75 PDD312 pKa = 3.97 TTLHH316 pKa = 6.61 FPQASEE322 pKa = 5.2 DD323 pKa = 3.8 VLQAPDD329 pKa = 5.37 LDD331 pKa = 4.15 FQDD334 pKa = 3.7 VGTLHH339 pKa = 6.77 RR340 pKa = 11.84 PIYY343 pKa = 8.41 STEE346 pKa = 3.64 GGYY349 pKa = 10.54 VRR351 pKa = 11.84 VSRR354 pKa = 11.84 FGEE357 pKa = 3.95 RR358 pKa = 11.84 EE359 pKa = 3.98 TIRR362 pKa = 11.84 TRR364 pKa = 11.84 SGAAIGARR372 pKa = 11.84 VHH374 pKa = 7.1 FYY376 pKa = 10.8 TDD378 pKa = 3.65 LSSIQSFAEE387 pKa = 3.74 QLPSVGSLGPDD398 pKa = 3.06 VADD401 pKa = 4.55 PGIEE405 pKa = 3.61 LHH407 pKa = 6.88 LFGEE411 pKa = 5.1 GTGDD415 pKa = 3.37 TSIADD420 pKa = 3.64 AQGGGVSLSNGTLHH434 pKa = 6.87 TEE436 pKa = 4.24 TEE438 pKa = 4.22 FTNASNGSLHH448 pKa = 6.81 SEE450 pKa = 4.32 YY451 pKa = 10.77 SNSMLLDD458 pKa = 3.9 SYY460 pKa = 11.63 TEE462 pKa = 4.22 TFNDD466 pKa = 3.41 AQLALMDD473 pKa = 4.73 SEE475 pKa = 5.5 GSTQVLSIPEE485 pKa = 4.02 LARR488 pKa = 11.84 PVRR491 pKa = 11.84 GFAEE495 pKa = 4.47 STGGLSVSYY504 pKa = 9.51 PVDD507 pKa = 3.5 MEE509 pKa = 4.14 ISGSSTSFIHH519 pKa = 6.8 NIPGPPSILLFYY531 pKa = 9.82 PDD533 pKa = 4.64 SSPSFYY539 pKa = 10.86 LHH541 pKa = 7.07 PSLLRR546 pKa = 11.84 RR547 pKa = 11.84 KK548 pKa = 9.49 RR549 pKa = 11.84 KK550 pKa = 9.18 RR551 pKa = 11.84 VFYY554 pKa = 10.92
Molecular weight: 59.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.035
IPC2_protein 5.041
IPC_protein 5.016
Toseland 4.991
ProMoST 5.169
Dawson 5.029
Bjellqvist 5.143
Wikipedia 4.914
Rodwell 4.94
Grimsley 4.927
Solomon 5.029
Lehninger 4.991
Nozaki 5.156
DTASelect 5.321
Thurlkill 4.991
EMBOSS 4.978
Sillero 5.232
Patrickios 4.037
IPC_peptide 5.041
IPC2_peptide 5.232
IPC2.peptide.svr19 5.134
Protein with the highest isoelectric point:
>tr|Q8UZ16|Q8UZ16_PSPVP E4 protein (Fragment) OS=Phocoena spinipinnis papillomavirus (isolate Burmeister's porpoise/Peru/PsPV1) OX=654916 GN=e4 PE=4 SV=1
TT1 pKa = 7.19 AQRR4 pKa = 11.84 CSCWQRR10 pKa = 11.84 KK11 pKa = 7.39 SAFLALFSFVCICAYY26 pKa = 9.64 LCTYY30 pKa = 9.55 ISLYY34 pKa = 9.98 IALL37 pKa = 4.57
Molecular weight: 4.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.031
IPC2_protein 7.717
IPC_protein 7.702
Toseland 7.029
ProMoST 8.141
Dawson 8.273
Bjellqvist 8.756
Wikipedia 8.17
Rodwell 8.258
Grimsley 6.927
Solomon 8.434
Lehninger 8.448
Nozaki 9.048
DTASelect 8.39
Thurlkill 8.434
EMBOSS 8.492
Sillero 8.814
Patrickios 0.006
IPC_peptide 8.419
IPC2_peptide 8.653
IPC2.peptide.svr19 8.786
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
0
8
2558
34
642
319.8
35.4
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.646 ± 0.571
2.658 ± 0.772
5.786 ± 0.629
5.786 ± 0.403
3.753 ± 0.484
7.193 ± 0.814
2.737 ± 0.64
3.714 ± 0.386
4.457 ± 0.962
8.835 ± 0.842
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.798 ± 0.336
3.245 ± 0.519
7.545 ± 0.984
3.909 ± 0.398
5.708 ± 0.396
9.03 ± 0.927
6.255 ± 0.311
6.216 ± 0.425
1.642 ± 0.348
3.088 ± 0.308
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here