Hymenobacter sp. DG25B

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Hymenobacteraceae; Hymenobacter; unclassified Hymenobacter

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3153 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A7LSZ4|A0A0A7LSZ4_9BACT Uncharacterized protein OS=Hymenobacter sp. DG25B OX=1385664 GN=PK28_18500 PE=4 SV=1
MM1 pKa = 7.51RR2 pKa = 11.84QACEE6 pKa = 3.72QQLNTVYY13 pKa = 10.49EE14 pKa = 4.0VDD16 pKa = 3.32ADD18 pKa = 3.66SRR20 pKa = 11.84VYY22 pKa = 10.77VDD24 pKa = 3.34QLIYY28 pKa = 10.57LAWLWANDD36 pKa = 3.25FVFYY40 pKa = 10.44DD41 pKa = 4.29EE42 pKa = 4.98RR43 pKa = 11.84DD44 pKa = 3.41GMHH47 pKa = 6.32WVDD50 pKa = 4.43GRR52 pKa = 11.84YY53 pKa = 9.76EE54 pKa = 4.07FDD56 pKa = 5.08NGWFDD61 pKa = 5.06NNIIDD66 pKa = 4.59NIYY69 pKa = 10.71SEE71 pKa = 4.7YY72 pKa = 10.82EE73 pKa = 3.61LDD75 pKa = 3.97KK76 pKa = 11.09LVPLLRR82 pKa = 11.84EE83 pKa = 3.93PAASTEE89 pKa = 4.06

Molecular weight: 10.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A7LJK0|A0A0A7LJK0_9BACT Uncharacterized protein OS=Hymenobacter sp. DG25B OX=1385664 GN=PK28_07285 PE=3 SV=1
MM1 pKa = 7.84PLLNGIINLPSAILPHH17 pKa = 5.89RR18 pKa = 11.84HH19 pKa = 6.42IIHH22 pKa = 4.84VVIRR26 pKa = 11.84IIRR29 pKa = 11.84LLLKK33 pKa = 10.35LHH35 pKa = 5.36VHH37 pKa = 6.58LLVSGPAAASNVSRR51 pKa = 11.84FARR54 pKa = 11.84RR55 pKa = 11.84IVVLVV60 pKa = 3.43

Molecular weight: 6.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3153

0

3153

1029997

52

2485

326.7

36.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.936 ± 0.054

0.714 ± 0.013

4.865 ± 0.032

5.645 ± 0.044

4.192 ± 0.025

7.392 ± 0.044

2.141 ± 0.026

4.742 ± 0.035

4.309 ± 0.038

10.928 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.091 ± 0.021

3.82 ± 0.039

5.037 ± 0.03

4.815 ± 0.04

5.911 ± 0.041

5.49 ± 0.035

6.128 ± 0.049

7.09 ± 0.031

1.196 ± 0.018

3.558 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski