Lactobacillus thailandensis DSM 22698 = JCM 13996

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Lacticaseibacillus; Lacticaseibacillus thailandensis

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1860 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0R2C7R1|A0A0R2C7R1_9LACO C-di-GMP-specific phosphodiesterase OS=Lactobacillus thailandensis DSM 22698 = JCM 13996 OX=1423810 GN=FD19_GL001104 PE=4 SV=1
MM1 pKa = 7.95LSPQVQNVAASTATAGSTTEE21 pKa = 4.04EE22 pKa = 4.05TSGITNTSVAHH33 pKa = 6.36SSADD37 pKa = 3.34VKK39 pKa = 11.3ASVASTSSATVVNTGNDD56 pKa = 3.17SSTATSATSTASASTSVSAVASATNGSAEE85 pKa = 4.17SLAATSAATAATTSVAVDD103 pKa = 3.94DD104 pKa = 4.93SSAALPASSAATAGAEE120 pKa = 4.29TSSATSGSSAASAQTVTSVAHH141 pKa = 4.67QALRR145 pKa = 11.84TRR147 pKa = 11.84SLASSAAPADD157 pKa = 4.05SEE159 pKa = 4.48VTTAINSGAANAPATVSYY177 pKa = 10.87HH178 pKa = 6.11NGSTQPTISVMVSGNFQAGDD198 pKa = 3.66VVTVYY203 pKa = 11.26LDD205 pKa = 3.49TTAMSVTAATTVKK218 pKa = 10.4GATEE222 pKa = 4.3SNVDD226 pKa = 2.93VSTNDD231 pKa = 2.63GRR233 pKa = 11.84TVKK236 pKa = 10.26GAQYY240 pKa = 10.61QFSSDD245 pKa = 3.57YY246 pKa = 10.94NGAFPVVFVLNGTADD261 pKa = 3.65DD262 pKa = 4.34QINNGTSTATIAVDD276 pKa = 3.22VDD278 pKa = 3.77KK279 pKa = 11.5NGTIVATNSVVLDD292 pKa = 3.58KK293 pKa = 10.82SYY295 pKa = 10.48TMTQPNDD302 pKa = 3.62TIRR305 pKa = 11.84RR306 pKa = 11.84NILNGTNGVTAGVIAQGAVTSEE328 pKa = 3.92LTLRR332 pKa = 11.84AQLQGLQYY340 pKa = 10.69FGDD343 pKa = 3.94YY344 pKa = 8.83STLVNSSPYY353 pKa = 10.35EE354 pKa = 4.0KK355 pKa = 9.79LTAASTVTFDD365 pKa = 6.0FSDD368 pKa = 3.51QLKK371 pKa = 10.21VDD373 pKa = 4.02SAVLSDD379 pKa = 4.17DD380 pKa = 3.84AVATGVTLTRR390 pKa = 11.84SGNSVTLTLPSGLTLAAISKK410 pKa = 9.8LAGYY414 pKa = 8.69LQVGLLPVTVPTTSTRR430 pKa = 11.84YY431 pKa = 9.63VLPTLTATWTIDD443 pKa = 3.13GQTNTVTEE451 pKa = 4.51PGNSGSSHH459 pKa = 6.3ANEE462 pKa = 3.7FAYY465 pKa = 10.64NIQTYY470 pKa = 7.62TAVGTVTVSSSSSYY484 pKa = 11.08LGLLQQGTDD493 pKa = 3.19NDD495 pKa = 4.39YY496 pKa = 10.83GLQWVVANEE505 pKa = 4.32SNFASGTTTTGSLSITSSPDD525 pKa = 2.86NSVGITSVNGLDD537 pKa = 3.88GNYY540 pKa = 7.86LTSAGLSKK548 pKa = 10.96NLTITFTTADD558 pKa = 3.63GQSQSFEE565 pKa = 4.31YY566 pKa = 11.11VDD568 pKa = 3.6GTTSYY573 pKa = 11.08VATVGSATDD582 pKa = 3.68PVTQVQIVDD591 pKa = 4.04HH592 pKa = 6.77AAQTAPYY599 pKa = 7.97VTGSISHH606 pKa = 6.1LQVGLIAYY614 pKa = 9.29GDD616 pKa = 3.77EE617 pKa = 4.31KK618 pKa = 9.81TTVTATADD626 pKa = 3.17TSLYY630 pKa = 11.0DD631 pKa = 3.67GDD633 pKa = 4.31GKK635 pKa = 11.11LVLDD639 pKa = 4.43TASSTDD645 pKa = 3.02SDD647 pKa = 4.14VVRR650 pKa = 11.84EE651 pKa = 4.01RR652 pKa = 11.84TVTKK656 pKa = 9.67TYY658 pKa = 10.42YY659 pKa = 8.19PQPSIVLSAGSNGVDD674 pKa = 3.01NQQGNTAQQIYY685 pKa = 10.71NPGDD689 pKa = 3.55EE690 pKa = 4.4LTEE693 pKa = 4.31SVNLLDD699 pKa = 4.95GGTNSGSASDD709 pKa = 3.52QVGRR713 pKa = 11.84IGNGDD718 pKa = 3.51GKK720 pKa = 10.56QPAVEE725 pKa = 4.67AVAAQNPMTIVVVAPTNTDD744 pKa = 3.49FADD747 pKa = 4.09DD748 pKa = 3.76ATLLSTLNKK757 pKa = 10.16SDD759 pKa = 3.33ISSSGDD765 pKa = 3.41LEE767 pKa = 4.2ITHH770 pKa = 7.15LDD772 pKa = 3.82DD773 pKa = 3.65VDD775 pKa = 3.81GRR777 pKa = 11.84PVIAITGVDD786 pKa = 3.67HH787 pKa = 5.71YY788 pKa = 9.22TASSRR793 pKa = 11.84LPIVLQVNADD803 pKa = 4.0AIGGSTISALNTIYY817 pKa = 11.2VMPSAVLSQYY827 pKa = 11.71NNGTILTSDD836 pKa = 3.59GSLATDD842 pKa = 3.72WATGNMYY849 pKa = 10.68NLSTGGSQLGTIITASSFTANASITGSADD878 pKa = 2.58SSAQVQTGTINVDD891 pKa = 3.03DD892 pKa = 4.83DD893 pKa = 3.67QATGKK898 pKa = 6.27TTLYY902 pKa = 10.86NGTTNPVTDD911 pKa = 3.41TLTTVNLVDD920 pKa = 4.47ADD922 pKa = 3.81GNQYY926 pKa = 8.16ITLTGPVQVVDD937 pKa = 3.9DD938 pKa = 4.8AGNTVAAAVTYY949 pKa = 7.38YY950 pKa = 11.03QNGAVVTDD958 pKa = 3.93PTQADD963 pKa = 3.97SFTVSGVSLAKK974 pKa = 9.97KK975 pKa = 10.1GSYY978 pKa = 10.44AVISYY983 pKa = 10.24GLTVVPSQLAGALTNKK999 pKa = 7.7TWAYY1003 pKa = 6.81QTSTQGYY1010 pKa = 5.64TAGTVATGNLVPLGAAISNTLVLQAIRR1037 pKa = 11.84DD1038 pKa = 4.02LTDD1041 pKa = 2.43TWYY1044 pKa = 10.33VAQRR1048 pKa = 11.84KK1049 pKa = 9.29ADD1051 pKa = 3.46GTYY1054 pKa = 10.46VVDD1057 pKa = 3.76SAVAAVTTTGVVGRR1071 pKa = 11.84IYY1073 pKa = 10.86NGYY1076 pKa = 7.5TLMGTSAADD1085 pKa = 3.1VGQAYY1090 pKa = 10.28GDD1092 pKa = 4.15DD1093 pKa = 3.88GAVLVGVYY1101 pKa = 10.29DD1102 pKa = 3.6ATTGQMVADD1111 pKa = 4.65GSMITYY1117 pKa = 10.32AEE1119 pKa = 4.11QVNSDD1124 pKa = 3.57GTITGHH1130 pKa = 5.76TYY1132 pKa = 11.26YY1133 pKa = 10.95LVTEE1137 pKa = 4.12QDD1139 pKa = 3.42VATKK1143 pKa = 10.79LNDD1146 pKa = 3.13VTVTYY1151 pKa = 10.55NGQTAASEE1159 pKa = 4.47VAGLQATANKK1169 pKa = 9.77VNVGLTSDD1177 pKa = 5.21DD1178 pKa = 3.21ITVVKK1183 pKa = 10.7DD1184 pKa = 3.74GINAGTYY1191 pKa = 8.91TYY1193 pKa = 11.03QLNAQGLAKK1202 pKa = 10.37LQAAVGNDD1210 pKa = 3.83AIVTDD1215 pKa = 3.92VGVTGTITIQPAVTTVTLNPGALTYY1240 pKa = 10.62DD1241 pKa = 3.14GRR1243 pKa = 11.84TPASGASLTATVAGQTLKK1261 pKa = 11.06LNSGDD1266 pKa = 3.44VTVADD1271 pKa = 4.78DD1272 pKa = 5.08GIDD1275 pKa = 3.35VGTYY1279 pKa = 9.01TYY1281 pKa = 11.18QLTAAGLSSLQSQVGSNYY1299 pKa = 9.79VVTTGTPGMIVISPEE1314 pKa = 3.83QMPVTLSSGSLVYY1327 pKa = 10.42DD1328 pKa = 4.38GKK1330 pKa = 9.4TKK1332 pKa = 10.87ASGAAVIATVNGEE1345 pKa = 4.31PLTLNDD1351 pKa = 5.4DD1352 pKa = 5.25DD1353 pKa = 4.2ITVTNDD1359 pKa = 3.87GINVGTYY1366 pKa = 10.1NYY1368 pKa = 10.43QLNQTGLEE1376 pKa = 4.03KK1377 pKa = 10.83LQTLVGDD1384 pKa = 4.13DD1385 pKa = 3.66FTVVVGDD1392 pKa = 4.47PGTITITPAVVDD1404 pKa = 3.71VVLNDD1409 pKa = 3.82GSFTYY1414 pKa = 10.46DD1415 pKa = 2.78GNTAASAAKK1424 pKa = 10.42GLVATVNGTPVDD1436 pKa = 4.38LDD1438 pKa = 3.54NSDD1441 pKa = 4.72IEE1443 pKa = 4.47VTDD1446 pKa = 4.59DD1447 pKa = 4.67GVDD1450 pKa = 3.22AGNYY1454 pKa = 9.6AYY1456 pKa = 10.49QLNDD1460 pKa = 3.17AGLAKK1465 pKa = 10.29VQLVVGSNYY1474 pKa = 9.37QVSVAVTGTITITPASITAALSDD1497 pKa = 4.11GGFTYY1502 pKa = 10.69DD1503 pKa = 3.36GATKK1507 pKa = 10.35ASEE1510 pKa = 4.28ATGLTATANSQAVDD1524 pKa = 3.71LSGEE1528 pKa = 4.42DD1529 pKa = 3.61IEE1531 pKa = 4.63VTNDD1535 pKa = 2.79GTGAGNYY1542 pKa = 9.65AYY1544 pKa = 10.13QLSDD1548 pKa = 3.09AGLAKK1553 pKa = 10.06VQSVVGTNYY1562 pKa = 9.75TVVLGGSGTVTIAQVVATTVXQSAVGPNYY1591 pKa = 9.78TVGVGDD1597 pKa = 4.13TGTITITPASITTTLSDD1614 pKa = 4.17GGFTYY1619 pKa = 10.71DD1620 pKa = 3.48GTTKK1624 pKa = 10.93ASGAPKK1630 pKa = 9.24LTATVVGQTVEE1641 pKa = 4.48LDD1643 pKa = 3.52SSDD1646 pKa = 4.41VDD1648 pKa = 3.8VTDD1651 pKa = 3.89NGSAAGDD1658 pKa = 3.44YY1659 pKa = 10.62VYY1661 pKa = 10.92QLNANGLEE1669 pKa = 4.38KK1670 pKa = 10.81VQSAAGANYY1679 pKa = 9.17IVRR1682 pKa = 11.84VGDD1685 pKa = 3.34TGTITIAKK1693 pKa = 9.71APATTTLSDD1702 pKa = 3.74GGFTYY1707 pKa = 10.71DD1708 pKa = 3.48GTTKK1712 pKa = 10.93ASGAPKK1718 pKa = 9.24LTATVVGQTVEE1729 pKa = 4.67LDD1731 pKa = 3.4SSDD1734 pKa = 3.09IDD1736 pKa = 4.18VINDD1740 pKa = 4.49AIAVGSYY1747 pKa = 10.35DD1748 pKa = 3.6YY1749 pKa = 11.14QLNATGLAKK1758 pKa = 10.47VQSAAGGNYY1767 pKa = 8.62TVGFGDD1773 pKa = 4.4AGTITITPASITTTLSDD1790 pKa = 4.17GGFTYY1795 pKa = 10.71DD1796 pKa = 3.48GTTKK1800 pKa = 10.93ASGAPKK1806 pKa = 9.34XFGDD1810 pKa = 4.38AGTITITPASITTTLSDD1827 pKa = 4.17GGFTYY1832 pKa = 10.71DD1833 pKa = 3.48GTTKK1837 pKa = 10.93ASGAPKK1843 pKa = 9.25LTATVIGQTVDD1854 pKa = 4.05LDD1856 pKa = 3.86SSDD1859 pKa = 4.12IDD1861 pKa = 3.52ITNDD1865 pKa = 2.45ATTAGNYY1872 pKa = 9.62DD1873 pKa = 3.63YY1874 pKa = 11.24QLNATGLAKK1883 pKa = 10.26VQSVVGPNYY1892 pKa = 9.58TVVLGGSGTVSISQVVATTVLSDD1915 pKa = 3.75GGFTFDD1921 pKa = 3.52GTTKK1925 pKa = 10.64ASEE1928 pKa = 4.37VTNLTASVVGQTVEE1942 pKa = 4.67LDD1944 pKa = 3.31SDD1946 pKa = 4.65DD1947 pKa = 5.94IIVTDD1952 pKa = 5.1DD1953 pKa = 3.28GSAAQDD1959 pKa = 2.63SWARR1963 pKa = 3.58

Molecular weight: 197.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0R2CGU8|A0A0R2CGU8_9LACO 50S ribosomal protein L30 OS=Lactobacillus thailandensis DSM 22698 = JCM 13996 OX=1423810 GN=rpmD PE=3 SV=1
MM1 pKa = 5.72TTKK4 pKa = 9.85RR5 pKa = 11.84TFQPKK10 pKa = 8.26KK11 pKa = 7.4RR12 pKa = 11.84HH13 pKa = 5.36RR14 pKa = 11.84ARR16 pKa = 11.84VHH18 pKa = 6.05GFMKK22 pKa = 10.54RR23 pKa = 11.84MSTKK27 pKa = 10.23NGRR30 pKa = 11.84HH31 pKa = 3.67VLARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84AKK39 pKa = 10.16GRR41 pKa = 11.84KK42 pKa = 9.04KK43 pKa = 9.67LTAA46 pKa = 4.2

Molecular weight: 5.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1860

0

1860

581517

46

2509

312.6

34.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.422 ± 0.079

0.416 ± 0.012

6.111 ± 0.06

4.387 ± 0.053

3.794 ± 0.037

7.368 ± 0.053

2.598 ± 0.031

5.897 ± 0.048

4.305 ± 0.062

9.561 ± 0.064

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.749 ± 0.03

3.965 ± 0.04

3.927 ± 0.038

4.673 ± 0.042

5.063 ± 0.047

5.334 ± 0.077

6.912 ± 0.078

8.147 ± 0.047

1.104 ± 0.022

3.264 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski