Simian retrovirus Y
Average proteome isoelectric point is 7.53
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|L0MZL7|L0MZL7_9RETR Envelope protein OS=Simian retrovirus Y OX=980635 GN=env PE=4 SV=1
MM1 pKa = 6.93 GQEE4 pKa = 4.61 LSQHH8 pKa = 6.05 DD9 pKa = 5.05 LYY11 pKa = 11.71 VDD13 pKa = 3.59 QLKK16 pKa = 10.53 KK17 pKa = 10.36 ALKK20 pKa = 10.08 ARR22 pKa = 11.84 GVKK25 pKa = 9.66 VKK27 pKa = 10.77 YY28 pKa = 10.54 ADD30 pKa = 3.61 LLKK33 pKa = 11.07 FFDD36 pKa = 4.81 FVKK39 pKa = 9.86 DD40 pKa = 3.41 TCPWFPQEE48 pKa = 3.67 GTIDD52 pKa = 3.17 IKK54 pKa = 10.13 RR55 pKa = 11.84 WRR57 pKa = 11.84 RR58 pKa = 11.84 VGDD61 pKa = 3.88 CFQDD65 pKa = 3.72 YY66 pKa = 10.54 YY67 pKa = 10.97 KK68 pKa = 10.65 TFGPEE73 pKa = 3.94 KK74 pKa = 10.38 VPVTAFSYY82 pKa = 10.07 WNLIKK87 pKa = 10.52 EE88 pKa = 4.72 LIDD91 pKa = 3.58 KK92 pKa = 11.04 KK93 pKa = 10.57 EE94 pKa = 3.93 DD95 pKa = 3.45 HH96 pKa = 6.89 PQVLAAVTQTEE107 pKa = 4.32 EE108 pKa = 4.02 LLKK111 pKa = 10.31 TGSQSDD117 pKa = 4.2 LQHH120 pKa = 6.76 LPPDD124 pKa = 4.34 PEE126 pKa = 4.22 TDD128 pKa = 4.57 LISLDD133 pKa = 3.76 SDD135 pKa = 3.72 EE136 pKa = 5.37 EE137 pKa = 4.4 GAKK140 pKa = 10.52 APSIKK145 pKa = 10.23 DD146 pKa = 3.32 CKK148 pKa = 8.53 TVRR151 pKa = 11.84 NKK153 pKa = 10.14 KK154 pKa = 9.18 PKK156 pKa = 9.96 RR157 pKa = 11.84 FPVLLTSQEE166 pKa = 4.27 SKK168 pKa = 11.28 NDD170 pKa = 3.74 DD171 pKa = 4.15 NDD173 pKa = 4.48 PDD175 pKa = 5.92 DD176 pKa = 6.28 DD177 pKa = 5.91 DD178 pKa = 5.84 PDD180 pKa = 4.0 PQEE183 pKa = 4.81 VNWDD187 pKa = 3.9 DD188 pKa = 4.26 LADD191 pKa = 3.74 AAARR195 pKa = 11.84 YY196 pKa = 8.02 HH197 pKa = 6.25 NPDD200 pKa = 2.99 WPPFLTRR207 pKa = 11.84 PPPYY211 pKa = 10.52 NKK213 pKa = 9.84 AAPSAPEE220 pKa = 3.67 VMAVVNPKK228 pKa = 10.56 EE229 pKa = 3.84 EE230 pKa = 4.31 LKK232 pKa = 11.05 EE233 pKa = 4.15 KK234 pKa = 10.21 IAQLEE239 pKa = 4.17 EE240 pKa = 4.15 QIKK243 pKa = 10.86 LEE245 pKa = 4.18 EE246 pKa = 4.12 LHH248 pKa = 6.4 QSLISRR254 pKa = 11.84 LQKK257 pKa = 10.7 LKK259 pKa = 10.02 TGNEE263 pKa = 3.69 RR264 pKa = 11.84 VTNLDD269 pKa = 3.24 NAGGFPRR276 pKa = 11.84 TPHH279 pKa = 6.86 WPRR282 pKa = 11.84 QHH284 pKa = 5.53 VPKK287 pKa = 10.5 GKK289 pKa = 10.24 SYY291 pKa = 10.85 TNIEE295 pKa = 4.33 RR296 pKa = 11.84 EE297 pKa = 4.2 PSPKK301 pKa = 10.32 DD302 pKa = 2.95 IFPVTEE308 pKa = 3.98 TVDD311 pKa = 3.43 AQGQAWRR318 pKa = 11.84 HH319 pKa = 5.12 HH320 pKa = 6.22 NGFDD324 pKa = 3.27 FTVIKK329 pKa = 9.85 EE330 pKa = 4.31 LKK332 pKa = 8.64 TAASQYY338 pKa = 10.77 GATAPYY344 pKa = 8.96 TLAIVEE350 pKa = 4.48 SVADD354 pKa = 3.63 NWLTPTDD361 pKa = 3.42 WNTLVRR367 pKa = 11.84 AVLSGGDD374 pKa = 3.36 HH375 pKa = 6.8 LLWKK379 pKa = 10.66 SEE381 pKa = 3.58 FFEE384 pKa = 4.44 NCRR387 pKa = 11.84 EE388 pKa = 3.98 TAKK391 pKa = 10.61 RR392 pKa = 11.84 NQQAGNGWDD401 pKa = 3.64 FDD403 pKa = 4.06 MLTGSGNYY411 pKa = 10.15 SNTDD415 pKa = 2.46 AQMQYY420 pKa = 11.44 DD421 pKa = 4.17 PGLFAQIQAAAIKK434 pKa = 9.62 AWRR437 pKa = 11.84 KK438 pKa = 9.78 LPVKK442 pKa = 9.92 GDD444 pKa = 3.67 PGASLTGVKK453 pKa = 9.76 QGPDD457 pKa = 3.08 EE458 pKa = 4.48 PFSEE462 pKa = 4.91 FVHH465 pKa = 7.31 RR466 pKa = 11.84 LITTAGRR473 pKa = 11.84 IFGNAEE479 pKa = 3.61 AGVDD483 pKa = 3.84 YY484 pKa = 10.87 VKK486 pKa = 10.86 QLAYY490 pKa = 10.49 EE491 pKa = 4.22 NANPACQAAIRR502 pKa = 11.84 PYY504 pKa = 10.38 RR505 pKa = 11.84 KK506 pKa = 8.54 KK507 pKa = 9.28 TDD509 pKa = 3.14 LTGYY513 pKa = 9.9 IRR515 pKa = 11.84 LCSDD519 pKa = 3.8 IGPSYY524 pKa = 10.24 QQGLAMAAAFSGQTVKK540 pKa = 11.11 DD541 pKa = 3.64 FLNNKK546 pKa = 8.72 HH547 pKa = 5.96 RR548 pKa = 11.84 EE549 pKa = 3.78 KK550 pKa = 10.93 GGCFKK555 pKa = 10.7 CGKK558 pKa = 9.61 AGHH561 pKa = 6.02 FAKK564 pKa = 10.59 NCQEE568 pKa = 4.14 NIKK571 pKa = 10.95 NPGTKK576 pKa = 9.98 VPGVCPRR583 pKa = 11.84 CKK585 pKa = 9.94 RR586 pKa = 11.84 GKK588 pKa = 9.48 HH589 pKa = 4.71 WANEE593 pKa = 4.47 CKK595 pKa = 10.49 SKK597 pKa = 9.96 TDD599 pKa = 3.38 SQGNPLPPHH608 pKa = 6.29 QGNGWRR614 pKa = 11.84 GQPQAPKK621 pKa = 9.09 QAYY624 pKa = 8.28 GAVSFVPANNNNPFQNLLEE643 pKa = 4.1 PHH645 pKa = 6.15 QEE647 pKa = 4.15 VQDD650 pKa = 3.85 WTSVPPPTQYY660 pKa = 11.76
Molecular weight: 73.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.209
IPC2_protein 6.198
IPC_protein 6.275
Toseland 6.504
ProMoST 6.707
Dawson 6.634
Bjellqvist 6.62
Wikipedia 6.649
Rodwell 6.62
Grimsley 6.634
Solomon 6.634
Lehninger 6.634
Nozaki 6.912
DTASelect 7.059
Thurlkill 7.088
EMBOSS 7.073
Sillero 7.029
Patrickios 4.38
IPC_peptide 6.649
IPC2_peptide 6.781
IPC2.peptide.svr19 6.787
Protein with the highest isoelectric point:
>tr|L0MXX7|L0MXX7_9RETR Gag protein OS=Simian retrovirus Y OX=980635 GN=gag PE=4 SV=1
SS1 pKa = 7.13 RR2 pKa = 11.84 KK3 pKa = 8.55 STPTPSGKK11 pKa = 9.56 RR12 pKa = 11.84 VEE14 pKa = 4.81 GPAPGPEE21 pKa = 3.72 TSLWGGQLCPSQQQQSISEE40 pKa = 4.33 LARR43 pKa = 11.84 ATPGSAGLDD52 pKa = 3.4 LSSASHH58 pKa = 5.85 TVLTPEE64 pKa = 5.01 MGPQALGTGIYY75 pKa = 10.61 GPLPTNTFGLIVGRR89 pKa = 11.84 SSITMKK95 pKa = 10.56 GLQVFPGIIDD105 pKa = 3.44 NDD107 pKa = 3.65 YY108 pKa = 9.78 TGEE111 pKa = 3.96 IKK113 pKa = 10.46 IMAKK117 pKa = 10.17 ALNNIITIPQGDD129 pKa = 4.5 RR130 pKa = 11.84 IAQLVLLPLIKK141 pKa = 9.87 TSNKK145 pKa = 8.09 VQQSFRR151 pKa = 11.84 GQKK154 pKa = 10.43 GFGSSDD160 pKa = 3.44 IYY162 pKa = 10.15 WVQPITNKK170 pKa = 10.15 KK171 pKa = 9.74 PSLTLWLDD179 pKa = 3.53 GKK181 pKa = 11.13 SFTGLMDD188 pKa = 3.6 TGADD192 pKa = 3.3 VTIIKK197 pKa = 10.34 QEE199 pKa = 3.87 DD200 pKa = 4.07 WPPNWPTTEE209 pKa = 3.83 TLTNLRR215 pKa = 11.84 GIGQSNNPRR224 pKa = 11.84 QSSKK228 pKa = 11.03 YY229 pKa = 8.55 LTWKK233 pKa = 10.59 DD234 pKa = 3.32 KK235 pKa = 11.17 EE236 pKa = 4.36 NNTGLIKK243 pKa = 10.5 PFIIPNLPVNLWGRR257 pKa = 11.84 DD258 pKa = 3.49 LLSQMKK264 pKa = 10.38 IMMCSPNDD272 pKa = 3.42 VVTAQMLAQGYY283 pKa = 9.59 SPGKK287 pKa = 10.3 GLGKK291 pKa = 10.29 NEE293 pKa = 4.59 DD294 pKa = 4.75 GILQPIPNLGQFDD307 pKa = 4.08 KK308 pKa = 11.24 KK309 pKa = 11.24 GLGNFF314 pKa = 3.79
Molecular weight: 33.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.877
IPC2_protein 8.624
IPC_protein 8.536
Toseland 9.472
ProMoST 9.107
Dawson 9.677
Bjellqvist 9.341
Wikipedia 9.794
Rodwell 10.16
Grimsley 9.706
Solomon 9.721
Lehninger 9.706
Nozaki 9.545
DTASelect 9.297
Thurlkill 9.531
EMBOSS 9.882
Sillero 9.619
Patrickios 7.644
IPC_peptide 9.721
IPC2_peptide 7.805
IPC2.peptide.svr19 7.877
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
2423
314
864
605.8
67.29
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.181 ± 0.845
2.064 ± 0.544
5.489 ± 0.561
3.673 ± 0.665
3.673 ± 0.387
6.48 ± 1.178
2.435 ± 0.542
6.191 ± 0.99
6.521 ± 0.759
10.194 ± 0.99
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.197 ± 0.345
5.076 ± 0.221
7.222 ± 0.475
6.356 ± 0.257
3.591 ± 0.222
6.067 ± 0.939
6.686 ± 0.464
5.241 ± 0.501
1.94 ± 0.275
2.724 ± 0.274
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here