Porcine circovirus 2 (PCV2)
Average proteome isoelectric point is 9.31
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q5QHY2|Q5QHY2_PCV2 27.9 kDa capsid protein OS=Porcine circovirus 2 OX=85708 GN=Cap PE=3 SV=1
MM1 pKa = 7.46 TYY3 pKa = 9.97 PRR5 pKa = 11.84 RR6 pKa = 11.84 RR7 pKa = 11.84 YY8 pKa = 8.69 RR9 pKa = 11.84 RR10 pKa = 11.84 RR11 pKa = 11.84 RR12 pKa = 11.84 HH13 pKa = 5.74 RR14 pKa = 11.84 PRR16 pKa = 11.84 SHH18 pKa = 7.16 LGQILRR24 pKa = 11.84 RR25 pKa = 11.84 RR26 pKa = 11.84 PWLVHH31 pKa = 4.91 PRR33 pKa = 11.84 HH34 pKa = 6.49 RR35 pKa = 11.84 YY36 pKa = 6.63 RR37 pKa = 11.84 WRR39 pKa = 11.84 RR40 pKa = 11.84 KK41 pKa = 9.16 NGIFNTRR48 pKa = 11.84 LSRR51 pKa = 11.84 TFGYY55 pKa = 8.51 TIKK58 pKa = 9.94 RR59 pKa = 11.84 TTVKK63 pKa = 9.57 TPSWAVDD70 pKa = 2.85 MMRR73 pKa = 11.84 FNINDD78 pKa = 3.93 FLPPGGGSNPRR89 pKa = 11.84 SVPFEE94 pKa = 3.82 YY95 pKa = 10.9 YY96 pKa = 10.3 RR97 pKa = 11.84 IRR99 pKa = 11.84 KK100 pKa = 8.77 VKK102 pKa = 10.6 VEE104 pKa = 4.87 FWPCSPITQGDD115 pKa = 3.72 RR116 pKa = 11.84 GVGSSAVILDD126 pKa = 3.93 DD127 pKa = 4.47 NFVTKK132 pKa = 9.83 ATALTYY138 pKa = 10.54 DD139 pKa = 3.69 PYY141 pKa = 11.23 VNYY144 pKa = 10.43 SSRR147 pKa = 11.84 HH148 pKa = 5.56 TITQPFSYY156 pKa = 9.51 HH157 pKa = 5.32 SRR159 pKa = 11.84 YY160 pKa = 7.59 FTPKK164 pKa = 9.45 PVLDD168 pKa = 3.46 STIDD172 pKa = 3.44 YY173 pKa = 7.55 FQPNNKK179 pKa = 9.15 RR180 pKa = 11.84 NQLWLRR186 pKa = 11.84 LQTTGNVDD194 pKa = 3.27 HH195 pKa = 7.02 VGLGTAFEE203 pKa = 4.08 NSIYY207 pKa = 10.28 DD208 pKa = 3.1 QEE210 pKa = 4.38 YY211 pKa = 8.85 NIRR214 pKa = 11.84 VTMYY218 pKa = 9.33 VQFRR222 pKa = 11.84 EE223 pKa = 4.54 FNLKK227 pKa = 10.36 DD228 pKa = 3.49 PPLNPP233 pKa = 4.04
Molecular weight: 27.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.311
IPC2_protein 10.116
IPC_protein 11.023
Toseland 10.789
ProMoST 10.76
Dawson 10.921
Bjellqvist 10.745
Wikipedia 11.228
Rodwell 10.95
Grimsley 11.008
Solomon 11.096
Lehninger 11.038
Nozaki 10.76
DTASelect 10.745
Thurlkill 10.818
EMBOSS 11.213
Sillero 10.862
Patrickios 10.584
IPC_peptide 11.096
IPC2_peptide 9.897
IPC2.peptide.svr19 8.104
Protein with the highest isoelectric point:
>tr|Q5QHY2|Q5QHY2_PCV2 27.9 kDa capsid protein OS=Porcine circovirus 2 OX=85708 GN=Cap PE=3 SV=1
MM1 pKa = 7.46 TYY3 pKa = 9.97 PRR5 pKa = 11.84 RR6 pKa = 11.84 RR7 pKa = 11.84 YY8 pKa = 8.69 RR9 pKa = 11.84 RR10 pKa = 11.84 RR11 pKa = 11.84 RR12 pKa = 11.84 HH13 pKa = 5.74 RR14 pKa = 11.84 PRR16 pKa = 11.84 SHH18 pKa = 7.16 LGQILRR24 pKa = 11.84 RR25 pKa = 11.84 RR26 pKa = 11.84 PWLVHH31 pKa = 4.91 PRR33 pKa = 11.84 HH34 pKa = 6.49 RR35 pKa = 11.84 YY36 pKa = 6.63 RR37 pKa = 11.84 WRR39 pKa = 11.84 RR40 pKa = 11.84 KK41 pKa = 9.16 NGIFNTRR48 pKa = 11.84 LSRR51 pKa = 11.84 TFGYY55 pKa = 8.51 TIKK58 pKa = 9.94 RR59 pKa = 11.84 TTVKK63 pKa = 9.57 TPSWAVDD70 pKa = 2.85 MMRR73 pKa = 11.84 FNINDD78 pKa = 3.93 FLPPGGGSNPRR89 pKa = 11.84 SVPFEE94 pKa = 3.82 YY95 pKa = 10.9 YY96 pKa = 10.3 RR97 pKa = 11.84 IRR99 pKa = 11.84 KK100 pKa = 8.77 VKK102 pKa = 10.6 VEE104 pKa = 4.87 FWPCSPITQGDD115 pKa = 3.72 RR116 pKa = 11.84 GVGSSAVILDD126 pKa = 3.93 DD127 pKa = 4.47 NFVTKK132 pKa = 9.83 ATALTYY138 pKa = 10.54 DD139 pKa = 3.69 PYY141 pKa = 11.23 VNYY144 pKa = 10.43 SSRR147 pKa = 11.84 HH148 pKa = 5.56 TITQPFSYY156 pKa = 9.51 HH157 pKa = 5.32 SRR159 pKa = 11.84 YY160 pKa = 7.59 FTPKK164 pKa = 9.45 PVLDD168 pKa = 3.46 STIDD172 pKa = 3.44 YY173 pKa = 7.55 FQPNNKK179 pKa = 9.15 RR180 pKa = 11.84 NQLWLRR186 pKa = 11.84 LQTTGNVDD194 pKa = 3.27 HH195 pKa = 7.02 VGLGTAFEE203 pKa = 4.08 NSIYY207 pKa = 10.28 DD208 pKa = 3.1 QEE210 pKa = 4.38 YY211 pKa = 8.85 NIRR214 pKa = 11.84 VTMYY218 pKa = 9.33 VQFRR222 pKa = 11.84 EE223 pKa = 4.54 FNLKK227 pKa = 10.36 DD228 pKa = 3.49 PPLNPP233 pKa = 4.04
Molecular weight: 27.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.311
IPC2_protein 10.116
IPC_protein 11.023
Toseland 10.789
ProMoST 10.76
Dawson 10.921
Bjellqvist 10.745
Wikipedia 11.228
Rodwell 10.95
Grimsley 11.008
Solomon 11.096
Lehninger 11.038
Nozaki 10.76
DTASelect 10.745
Thurlkill 10.818
EMBOSS 11.213
Sillero 10.862
Patrickios 10.584
IPC_peptide 11.096
IPC2_peptide 9.897
IPC2.peptide.svr19 8.104
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
233
233
233
233.0
27.88
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
2.146 ± 0.0
0.429 ± 0.0
4.721 ± 0.0
2.146 ± 0.0
5.579 ± 0.0
5.15 ± 0.0
3.004 ± 0.0
4.721 ± 0.0
3.863 ± 0.0
6.009 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.717 ± 0.0
6.438 ± 0.0
8.155 ± 0.0
3.433 ± 0.0
13.305 ± 0.0
6.009 ± 0.0
8.155 ± 0.0
6.438 ± 0.0
2.146 ± 0.0
6.438 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here