Streptomyces sp. Tue6028

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6527 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2A3H5H0|A0A2A3H5H0_9ACTN RNA polymerase subunit sigma-24 OS=Streptomyces sp. Tue6028 OX=2036037 GN=BKI49_12635 PE=3 SV=1
MM1 pKa = 7.39SRR3 pKa = 11.84IAKK6 pKa = 8.22ATVVALGTGAVMLSGAGLAMADD28 pKa = 3.32AGAAGEE34 pKa = 4.37ADD36 pKa = 3.84GSPGAVSGNAVQAPIHH52 pKa = 6.1VPVNLCGDD60 pKa = 3.78TVDD63 pKa = 5.12VVGLLNPVFGNQCEE77 pKa = 4.27NAGTPEE83 pKa = 3.96EE84 pKa = 4.27NRR86 pKa = 11.84DD87 pKa = 3.45GGDD90 pKa = 3.54DD91 pKa = 3.23EE92 pKa = 5.99GYY94 pKa = 11.01GYY96 pKa = 11.41GSS98 pKa = 3.44

Molecular weight: 9.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2A3H1E1|A0A2A3H1E1_9ACTN Histidine kinase OS=Streptomyces sp. Tue6028 OX=2036037 GN=BKI49_22940 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILANRR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.51GRR40 pKa = 11.84ASLSAA45 pKa = 3.83

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6527

0

6527

2208326

37

2895

338.3

36.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.464 ± 0.042

0.765 ± 0.008

6.064 ± 0.024

5.626 ± 0.032

2.783 ± 0.021

9.595 ± 0.028

2.282 ± 0.014

3.111 ± 0.022

2.308 ± 0.027

10.288 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.736 ± 0.012

1.762 ± 0.014

5.994 ± 0.027

2.722 ± 0.016

7.862 ± 0.035

5.16 ± 0.021

6.151 ± 0.024

8.641 ± 0.025

1.528 ± 0.013

2.156 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski