Amino acid dipepetide frequency for Paenibacillus pasadenensis

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	18.04AlaAla: 18.04 ± 0.234	0.931AlaCys: 0.931 ± 0.025	6.073AlaAsp: 6.073 ± 0.08	8.283AlaGlu: 8.283 ± 0.099	3.708AlaPhe: 3.708 ± 0.05	11.18AlaGly: 11.18 ± 0.141	1.538AlaHis: 1.538 ± 0.033	4.919AlaIle: 4.919 ± 0.061	3.968AlaLys: 3.968 ± 0.054	11.113AlaLeu: 11.113 ± 0.111	2.742AlaMet: 2.742 ± 0.044	2.145AlaAsn: 2.145 ± 0.045	4.76AlaPro: 4.76 ± 0.17	3.061AlaGln: 3.061 ± 0.046	6.197AlaArg: 6.197 ± 0.085	7.45AlaSer: 7.45 ± 0.093	3.842AlaThr: 3.842 ± 0.066	7.913AlaVal: 7.913 ± 0.089	1.402AlaTrp: 1.402 ± 0.035	3.047AlaTyr: 3.047 ± 0.043	0.001AlaXaa: 0.001 ± 0.001
Cys	0.645CysAla: 0.645 ± 0.024	0.106CysCys: 0.106 ± 0.009	0.328CysAsp: 0.328 ± 0.017	0.379CysGlu: 0.379 ± 0.015	0.245CysPhe: 0.245 ± 0.012	0.862CysGly: 0.862 ± 0.026	0.148CysHis: 0.148 ± 0.009	0.309CysIle: 0.309 ± 0.014	0.191CysLys: 0.191 ± 0.011	0.708CysLeu: 0.708 ± 0.021	0.167CysMet: 0.167 ± 0.01	0.176CysAsn: 0.176 ± 0.012	0.368CysPro: 0.368 ± 0.017	0.156CysGln: 0.156 ± 0.01	0.638CysArg: 0.638 ± 0.023	0.549CysSer: 0.549 ± 0.019	0.323CysThr: 0.323 ± 0.017	0.408CysVal: 0.408 ± 0.018	0.094CysTrp: 0.094 ± 0.008	0.181CysTyr: 0.181 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	5.415AspAla: 5.415 ± 0.066	0.328AspCys: 0.328 ± 0.016	2.429AspAsp: 2.429 ± 0.041	3.916AspGlu: 3.916 ± 0.05	1.85AspPhe: 1.85 ± 0.04	5.493AspGly: 5.493 ± 0.08	0.809AspHis: 0.809 ± 0.024	2.745AspIle: 2.745 ± 0.046	2.179AspLys: 2.179 ± 0.047	4.62AspLeu: 4.62 ± 0.058	1.168AspMet: 1.168 ± 0.027	1.336AspAsn: 1.336 ± 0.031	2.87AspPro: 2.87 ± 0.048	1.356AspGln: 1.356 ± 0.031	3.635AspArg: 3.635 ± 0.053	2.764AspSer: 2.764 ± 0.048	2.066AspThr: 2.066 ± 0.038	3.247AspVal: 3.247 ± 0.048	0.895AspTrp: 0.895 ± 0.027	1.964AspTyr: 1.964 ± 0.038	0.0AspXaa: 0.0 ± 0.0
Glu	8.516GluAla: 8.516 ± 0.109	0.303GluCys: 0.303 ± 0.015	2.934GluAsp: 2.934 ± 0.051	5.439GluGlu: 5.439 ± 0.073	1.87GluPhe: 1.87 ± 0.035	4.996GluGly: 4.996 ± 0.066	1.384GluHis: 1.384 ± 0.029	3.241GluIle: 3.241 ± 0.051	2.902GluLys: 2.902 ± 0.044	8.764GluLeu: 8.764 ± 0.1	1.626GluMet: 1.626 ± 0.036	1.524GluAsn: 1.524 ± 0.035	3.511GluPro: 3.511 ± 0.06	3.37GluGln: 3.37 ± 0.049	6.055GluArg: 6.055 ± 0.1	3.577GluSer: 3.577 ± 0.048	3.103GluThr: 3.103 ± 0.04	3.584GluVal: 3.584 ± 0.055	0.912GluTrp: 0.912 ± 0.026	1.406GluTyr: 1.406 ± 0.035	0.0GluXaa: 0.0 ± 0.0
Phe	3.589PheAla: 3.589 ± 0.049	0.295PheCys: 0.295 ± 0.017	2.104PheAsp: 2.104 ± 0.043	2.142PheGlu: 2.142 ± 0.04	1.518PhePhe: 1.518 ± 0.037	3.21PheGly: 3.21 ± 0.046	0.762PheHis: 0.762 ± 0.021	1.893PheIle: 1.893 ± 0.04	1.435PheLys: 1.435 ± 0.031	3.535PheLeu: 3.535 ± 0.052	0.847PheMet: 0.847 ± 0.022	1.162PheAsn: 1.162 ± 0.032	1.489PhePro: 1.489 ± 0.029	1.137PheGln: 1.137 ± 0.029	2.476PheArg: 2.476 ± 0.045	2.349PheSer: 2.349 ± 0.037	1.875PheThr: 1.875 ± 0.039	2.587PheVal: 2.587 ± 0.037	0.476PheTrp: 0.476 ± 0.017	1.256PheTyr: 1.256 ± 0.03	0.0PheXaa: 0.0 ± 0.0
Gly	9.004GlyAla: 9.004 ± 0.113	0.823GlyCys: 0.823 ± 0.027	3.905GlyAsp: 3.905 ± 0.053	5.506GlyGlu: 5.506 ± 0.068	3.261GlyPhe: 3.261 ± 0.05	8.242GlyGly: 8.242 ± 0.132	1.454GlyHis: 1.454 ± 0.031	5.008GlyIle: 5.008 ± 0.063	4.042GlyLys: 4.042 ± 0.065	8.614GlyLeu: 8.614 ± 0.1	2.593GlyMet: 2.593 ± 0.044	2.152GlyAsn: 2.152 ± 0.042	2.812GlyPro: 2.812 ± 0.048	2.836GlyGln: 2.836 ± 0.047	5.972GlyArg: 5.972 ± 0.084	6.309GlySer: 6.309 ± 0.079	4.517GlyThr: 4.517 ± 0.06	5.423GlyVal: 5.423 ± 0.061	1.518GlyTrp: 1.518 ± 0.035	2.945GlyTyr: 2.945 ± 0.047	0.001GlyXaa: 0.001 ± 0.001
His	2.03HisAla: 2.03 ± 0.043	0.139HisCys: 0.139 ± 0.008	0.93HisAsp: 0.93 ± 0.025	1.177HisGlu: 1.177 ± 0.026	0.741HisPhe: 0.741 ± 0.026	1.683HisGly: 1.683 ± 0.033	0.496HisHis: 0.496 ± 0.018	0.803HisIle: 0.803 ± 0.023	0.533HisLys: 0.533 ± 0.018	1.824HisLeu: 1.824 ± 0.039	0.381HisMet: 0.381 ± 0.016	0.413HisAsn: 0.413 ± 0.018	1.366HisPro: 1.366 ± 0.034	0.541HisGln: 0.541 ± 0.018	1.241HisArg: 1.241 ± 0.03	0.928HisSer: 0.928 ± 0.027	0.766HisThr: 0.766 ± 0.024	1.249HisVal: 1.249 ± 0.032	0.248HisTrp: 0.248 ± 0.012	0.675HisTyr: 0.675 ± 0.02	0.0HisXaa: 0.0 ± 0.0
Ile	5.683IleAla: 5.683 ± 0.071	0.416IleCys: 0.416 ± 0.018	2.941IleAsp: 2.941 ± 0.041	3.38IleGlu: 3.38 ± 0.048	1.542IlePhe: 1.542 ± 0.036	5.032IleGly: 5.032 ± 0.063	0.992IleHis: 0.992 ± 0.027	2.26IleIle: 2.26 ± 0.049	1.776IleLys: 1.776 ± 0.038	4.262IleLeu: 4.262 ± 0.07	1.058IleMet: 1.058 ± 0.028	1.374IleAsn: 1.374 ± 0.034	2.38IlePro: 2.38 ± 0.043	1.679IleGln: 1.679 ± 0.037	4.009IleArg: 4.009 ± 0.055	2.894IleSer: 2.894 ± 0.052	2.225IleThr: 2.225 ± 0.047	4.401IleVal: 4.401 ± 0.062	0.56IleTrp: 0.56 ± 0.022	1.425IleTyr: 1.425 ± 0.033	0.0IleXaa: 0.0 ± 0.0
Lys	4.124LysAla: 4.124 ± 0.05	0.137LysCys: 0.137 ± 0.008	2.161LysAsp: 2.161 ± 0.046	3.106LysGlu: 3.106 ± 0.055	1.082LysPhe: 1.082 ± 0.026	3.105LysGly: 3.105 ± 0.046	0.743LysHis: 0.743 ± 0.024	1.85LysIle: 1.85 ± 0.043	1.942LysLys: 1.942 ± 0.047	4.547LysLeu: 4.547 ± 0.06	0.94LysMet: 0.94 ± 0.025	1.172LysAsn: 1.172 ± 0.033	2.191LysPro: 2.191 ± 0.046	1.685LysGln: 1.685 ± 0.04	2.689LysArg: 2.689 ± 0.043	2.193LysSer: 2.193 ± 0.044	2.153LysThr: 2.153 ± 0.043	2.445LysVal: 2.445 ± 0.046	0.49LysTrp: 0.49 ± 0.018	1.058LysTyr: 1.058 ± 0.028	0.0LysXaa: 0.0 ± 0.0
Leu	12.039LeuAla: 12.039 ± 0.099	0.774LeuCys: 0.774 ± 0.022	5.753LeuAsp: 5.753 ± 0.057	7.312LeuGlu: 7.312 ± 0.086	4.104LeuPhe: 4.104 ± 0.056	8.156LeuGly: 8.156 ± 0.081	2.1LeuHis: 2.1 ± 0.039	5.014LeuIle: 5.014 ± 0.068	4.053LeuLys: 4.053 ± 0.054	13.277LeuLeu: 13.277 ± 0.151	2.359LeuMet: 2.359 ± 0.043	2.736LeuAsn: 2.736 ± 0.047	5.645LeuPro: 5.645 ± 0.077	4.212LeuGln: 4.212 ± 0.056	7.24LeuArg: 7.24 ± 0.092	6.774LeuSer: 6.774 ± 0.07	5.234LeuThr: 5.234 ± 0.06	6.742LeuVal: 6.742 ± 0.073	1.139LeuTrp: 1.139 ± 0.032	3.05LeuTyr: 3.05 ± 0.049	0.0LeuXaa: 0.0 ± 0.0
Met	2.487MetAla: 2.487 ± 0.04	0.096MetCys: 0.096 ± 0.008	1.406MetAsp: 1.406 ± 0.031	1.718MetGlu: 1.718 ± 0.034	0.762MetPhe: 0.762 ± 0.023	1.617MetGly: 1.617 ± 0.032	0.372MetHis: 0.372 ± 0.014	1.28MetIle: 1.28 ± 0.032	1.491MetLys: 1.491 ± 0.028	2.796MetLeu: 2.796 ± 0.045	0.699MetMet: 0.699 ± 0.024	1.082MetAsn: 1.082 ± 0.023	1.216MetPro: 1.216 ± 0.031	0.86MetGln: 0.86 ± 0.024	1.548MetArg: 1.548 ± 0.033	1.547MetSer: 1.547 ± 0.032	1.54MetThr: 1.54 ± 0.033	1.317MetVal: 1.317 ± 0.029	0.187MetTrp: 0.187 ± 0.011	0.553MetTyr: 0.553 ± 0.02	0.0MetXaa: 0.0 ± 0.0
Asn	2.507AsnAla: 2.507 ± 0.047	0.155AsnCys: 0.155 ± 0.01	1.286AsnAsp: 1.286 ± 0.03	1.712AsnGlu: 1.712 ± 0.037	0.932AsnPhe: 0.932 ± 0.027	2.561AsnGly: 2.561 ± 0.046	0.472AsnHis: 0.472 ± 0.018	1.334AsnIle: 1.334 ± 0.032	1.053AsnLys: 1.053 ± 0.032	2.357AsnLeu: 2.357 ± 0.043	0.615AsnMet: 0.615 ± 0.019	0.836AsnAsn: 0.836 ± 0.033	1.586AsnPro: 1.586 ± 0.037	0.792AsnGln: 0.792 ± 0.027	1.827AsnArg: 1.827 ± 0.038	1.375AsnSer: 1.375 ± 0.035	1.284AsnThr: 1.284 ± 0.038	1.903AsnVal: 1.903 ± 0.038	0.368AsnTrp: 0.368 ± 0.016	0.882AsnTyr: 0.882 ± 0.026	0.0AsnXaa: 0.0 ± 0.0
Pro	6.057ProAla: 6.057 ± 0.103	0.298ProCys: 0.298 ± 0.014	3.157ProAsp: 3.157 ± 0.049	4.002ProGlu: 4.002 ± 0.064	1.834ProPhe: 1.834 ± 0.031	4.235ProGly: 4.235 ± 0.062	0.934ProHis: 0.934 ± 0.028	2.167ProIle: 2.167 ± 0.038	1.469ProLys: 1.469 ± 0.031	4.932ProLeu: 4.932 ± 0.068	1.027ProMet: 1.027 ± 0.024	1.126ProAsn: 1.126 ± 0.032	2.027ProPro: 2.027 ± 0.05	1.687ProGln: 1.687 ± 0.037	2.201ProArg: 2.201 ± 0.045	3.37ProSer: 3.37 ± 0.061	2.131ProThr: 2.131 ± 0.06	3.73ProVal: 3.73 ± 0.313	0.676ProTrp: 0.676 ± 0.027	1.466ProTyr: 1.466 ± 0.037	0.0ProXaa: 0.0 ± 0.0
Gln	4.38GlnAla: 4.38 ± 0.06	0.128GlnCys: 0.128 ± 0.009	1.559GlnAsp: 1.559 ± 0.032	2.435GlnGlu: 2.435 ± 0.042	1.135GlnPhe: 1.135 ± 0.027	2.652GlnGly: 2.652 ± 0.042	0.603GlnHis: 0.603 ± 0.018	1.65GlnIle: 1.65 ± 0.037	1.36GlnLys: 1.36 ± 0.029	4.258GlnLeu: 4.258 ± 0.058	0.828GlnMet: 0.828 ± 0.025	0.863GlnAsn: 0.863 ± 0.026	1.982GlnPro: 1.982 ± 0.042	1.66GlnGln: 1.66 ± 0.037	2.222GlnArg: 2.222 ± 0.047	1.843GlnSer: 1.843 ± 0.037	1.669GlnThr: 1.669 ± 0.032	2.151GlnVal: 2.151 ± 0.035	0.432GlnTrp: 0.432 ± 0.017	0.898GlnTyr: 0.898 ± 0.026	0.0GlnXaa: 0.0 ± 0.0
Arg	5.536ArgAla: 5.536 ± 0.072	0.485ArgCys: 0.485 ± 0.02	3.039ArgAsp: 3.039 ± 0.047	5.215ArgGlu: 5.215 ± 0.078	2.78ArgPhe: 2.78 ± 0.043	4.44ArgGly: 4.44 ± 0.066	1.446ArgHis: 1.446 ± 0.032	4.113ArgIle: 4.113 ± 0.055	2.821ArgLys: 2.821 ± 0.053	8.531ArgLeu: 8.531 ± 0.119	2.046ArgMet: 2.046 ± 0.034	1.514ArgAsn: 1.514 ± 0.032	3.086ArgPro: 3.086 ± 0.055	2.831ArgGln: 2.831 ± 0.045	5.376ArgArg: 5.376 ± 0.09	4.696ArgSer: 4.696 ± 0.068	3.313ArgThr: 3.313 ± 0.048	3.652ArgVal: 3.652 ± 0.057	1.019ArgTrp: 1.019 ± 0.031	2.104ArgTyr: 2.104 ± 0.036	0.0ArgXaa: 0.0 ± 0.0
Ser	6.795SerAla: 6.795 ± 0.078	0.454SerCys: 0.454 ± 0.018	2.837SerAsp: 2.837 ± 0.047	3.523SerGlu: 3.523 ± 0.052	2.696SerPhe: 2.696 ± 0.044	6.82SerGly: 6.82 ± 0.087	1.042SerHis: 1.042 ± 0.034	3.312SerIle: 3.312 ± 0.049	2.288SerLys: 2.288 ± 0.047	6.508SerLeu: 6.508 ± 0.076	1.597SerMet: 1.597 ± 0.03	1.434SerAsn: 1.434 ± 0.033	3.091SerPro: 3.091 ± 0.054	1.78SerGln: 1.78 ± 0.038	4.379SerArg: 4.379 ± 0.062	4.211SerSer: 4.211 ± 0.062	2.677SerThr: 2.677 ± 0.056	4.363SerVal: 4.363 ± 0.062	0.954SerTrp: 0.954 ± 0.032	2.01SerTyr: 2.01 ± 0.04	0.0SerXaa: 0.0 ± 0.0
Thr	5.281ThrAla: 5.281 ± 0.064	0.25ThrCys: 0.25 ± 0.013	2.513ThrAsp: 2.513 ± 0.042	2.681ThrGlu: 2.681 ± 0.04	1.884ThrPhe: 1.884 ± 0.04	4.435ThrGly: 4.435 ± 0.063	0.772ThrHis: 0.772 ± 0.019	2.781ThrIle: 2.781 ± 0.051	1.64ThrLys: 1.64 ± 0.038	5.007ThrLeu: 5.007 ± 0.051	1.182ThrMet: 1.182 ± 0.027	1.34ThrAsn: 1.34 ± 0.035	2.684ThrPro: 2.684 ± 0.067	1.16ThrGln: 1.16 ± 0.031	2.439ThrArg: 2.439 ± 0.045	2.725ThrSer: 2.725 ± 0.053	2.324ThrThr: 2.324 ± 0.053	3.925ThrVal: 3.925 ± 0.066	0.503ThrTrp: 0.503 ± 0.019	1.527ThrTyr: 1.527 ± 0.039	0.0ThrXaa: 0.0 ± 0.0
Val	5.994ValAla: 5.994 ± 0.15	0.566ValCys: 0.566 ± 0.019	3.467ValAsp: 3.467 ± 0.053	4.211ValGlu: 4.211 ± 0.055	2.499ValPhe: 2.499 ± 0.04	4.622ValGly: 4.622 ± 0.062	1.306ValHis: 1.306 ± 0.03	3.403ValIle: 3.403 ± 0.043	2.95ValLys: 2.95 ± 0.056	7.417ValLeu: 7.417 ± 0.076	1.569ValMet: 1.569 ± 0.033	1.947ValAsn: 1.947 ± 0.043	3.55ValPro: 3.55 ± 0.066	2.487ValGln: 2.487 ± 0.042	4.645ValArg: 4.645 ± 0.058	4.481ValSer: 4.481 ± 0.067	3.702ValThr: 3.702 ± 0.072	4.888ValVal: 4.888 ± 0.077	0.832ValTrp: 0.832 ± 0.024	2.159ValTyr: 2.159 ± 0.039	0.0ValXaa: 0.0 ± 0.0
Trp	1.038TrpAla: 1.038 ± 0.025	0.1TrpCys: 0.1 ± 0.009	0.625TrpAsp: 0.625 ± 0.021	0.779TrpGlu: 0.779 ± 0.023	0.497TrpPhe: 0.497 ± 0.018	0.873TrpGly: 0.873 ± 0.027	0.258TrpHis: 0.258 ± 0.015	0.813TrpIle: 0.813 ± 0.024	0.635TrpLys: 0.635 ± 0.019	1.682TrpLeu: 1.682 ± 0.041	0.43TrpMet: 0.43 ± 0.016	0.556TrpAsn: 0.556 ± 0.021	0.475TrpPro: 0.475 ± 0.016	0.545TrpGln: 0.545 ± 0.019	0.884TrpArg: 0.884 ± 0.025	0.995TrpSer: 0.995 ± 0.03	0.886TrpThr: 0.886 ± 0.025	0.671TrpVal: 0.671 ± 0.022	0.191TrpTrp: 0.191 ± 0.013	0.363TrpTyr: 0.363 ± 0.018	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.155TyrAla: 3.155 ± 0.047	0.201TyrCys: 0.201 ± 0.012	1.638TyrAsp: 1.638 ± 0.035	2.103TyrGlu: 2.103 ± 0.036	1.194TyrPhe: 1.194 ± 0.028	2.723TyrGly: 2.723 ± 0.047	0.534TyrHis: 0.534 ± 0.016	1.395TyrIle: 1.395 ± 0.031	1.085TyrLys: 1.085 ± 0.028	2.959TyrLeu: 2.959 ± 0.047	0.697TyrMet: 0.697 ± 0.021	0.949TyrAsn: 0.949 ± 0.029	1.435TyrPro: 1.435 ± 0.035	0.873TyrGln: 0.873 ± 0.022	2.372TyrArg: 2.372 ± 0.04	1.74TyrSer: 1.74 ± 0.04	1.423TyrThr: 1.423 ± 0.039	2.096TyrVal: 2.096 ± 0.031	0.4TyrTrp: 0.4 ± 0.016	1.064TyrTyr: 1.064 ± 0.032	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.001XaaPro: 0.001 ± 0.001	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.001XaaTyr: 0.001 ± 0.001	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4980 proteins (1626338 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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