Sewage-associated circular DNA virus-10

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 7.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A075J446|A0A075J446_9VIRU Capsid protein OS=Sewage-associated circular DNA virus-10 OX=1519386 PE=4 SV=1
MM1 pKa = 7.04SQNLEE6 pKa = 4.0SGRR9 pKa = 11.84RR10 pKa = 11.84EE11 pKa = 4.11ALKK14 pKa = 9.97MRR16 pKa = 11.84AWCFTHH22 pKa = 7.11NNFPYY27 pKa = 10.51AAADD31 pKa = 3.69LPLCKK36 pKa = 9.99DD37 pKa = 2.89EE38 pKa = 6.24RR39 pKa = 11.84YY40 pKa = 10.05VVWQHH45 pKa = 5.57EE46 pKa = 4.64KK47 pKa = 10.7VDD49 pKa = 3.61TDD51 pKa = 4.4HH52 pKa = 6.29IQGYY56 pKa = 9.76IEE58 pKa = 4.06LSKK61 pKa = 9.98PQRR64 pKa = 11.84ISAMIKK70 pKa = 8.6WLPGAHH76 pKa = 5.71FEE78 pKa = 4.35EE79 pKa = 5.13RR80 pKa = 11.84RR81 pKa = 11.84GTPDD85 pKa = 2.96QARR88 pKa = 11.84KK89 pKa = 10.14YY90 pKa = 10.79CMEE93 pKa = 4.25EE94 pKa = 3.82DD95 pKa = 3.74TRR97 pKa = 11.84VEE99 pKa = 4.47GPWEE103 pKa = 4.12RR104 pKa = 11.84GSYY107 pKa = 8.62GTTQGKK113 pKa = 10.06RR114 pKa = 11.84SDD116 pKa = 3.13IDD118 pKa = 3.61VVRR121 pKa = 11.84EE122 pKa = 4.38AIAAGADD129 pKa = 3.22RR130 pKa = 11.84RR131 pKa = 11.84EE132 pKa = 4.35VYY134 pKa = 9.84NAHH137 pKa = 6.6SDD139 pKa = 3.18IAAKK143 pKa = 9.81YY144 pKa = 7.76PRR146 pKa = 11.84YY147 pKa = 10.02VEE149 pKa = 3.95TMLRR153 pKa = 11.84FAKK156 pKa = 10.12EE157 pKa = 3.8DD158 pKa = 3.58AVDD161 pKa = 4.22KK162 pKa = 11.07ILVFEE167 pKa = 4.51PRR169 pKa = 11.84QGFQTDD175 pKa = 4.09LLDD178 pKa = 4.51MISGPADD185 pKa = 3.13SRR187 pKa = 11.84SIHH190 pKa = 5.14WVYY193 pKa = 11.47DD194 pKa = 3.47RR195 pKa = 11.84VGNNGKK201 pKa = 8.01TYY203 pKa = 9.04FAKK206 pKa = 10.91YY207 pKa = 10.3LVDD210 pKa = 3.82KK211 pKa = 11.1FGAFYY216 pKa = 10.56TNGGKK221 pKa = 9.79SVDD224 pKa = 3.27LAYY227 pKa = 10.25AYY229 pKa = 10.17GGEE232 pKa = 4.34SIVIFDD238 pKa = 3.74YY239 pKa = 11.36VRR241 pKa = 11.84DD242 pKa = 3.71AEE244 pKa = 4.4EE245 pKa = 3.86YY246 pKa = 9.18VGYY249 pKa = 10.63GVIEE253 pKa = 3.85QLKK256 pKa = 10.32NGIAMSTKK264 pKa = 9.9YY265 pKa = 10.88EE266 pKa = 4.59SITKK270 pKa = 9.99RR271 pKa = 11.84FNIPHH276 pKa = 6.34VIVLANFKK284 pKa = 9.51PQEE287 pKa = 4.27GKK289 pKa = 10.67FSSDD293 pKa = 3.55RR294 pKa = 11.84IKK296 pKa = 10.43MINVTT301 pKa = 3.77

Molecular weight: 34.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A075J446|A0A075J446_9VIRU Capsid protein OS=Sewage-associated circular DNA virus-10 OX=1519386 PE=4 SV=1
MM1 pKa = 6.67YY2 pKa = 10.15RR3 pKa = 11.84RR4 pKa = 11.84RR5 pKa = 11.84KK6 pKa = 6.37TARR9 pKa = 11.84KK10 pKa = 8.21SRR12 pKa = 11.84RR13 pKa = 11.84VKK15 pKa = 9.48TRR17 pKa = 11.84RR18 pKa = 11.84SSLGRR23 pKa = 11.84RR24 pKa = 11.84KK25 pKa = 9.85RR26 pKa = 11.84SLRR29 pKa = 11.84KK30 pKa = 8.5VSRR33 pKa = 11.84RR34 pKa = 11.84KK35 pKa = 9.8RR36 pKa = 11.84KK37 pKa = 7.64STPRR41 pKa = 11.84SGMKK45 pKa = 9.14MRR47 pKa = 11.84RR48 pKa = 11.84SVAKK52 pKa = 10.01SANPTMMDD60 pKa = 3.29VQAVAIEE67 pKa = 4.06TSKK70 pKa = 10.83RR71 pKa = 11.84SSARR75 pKa = 11.84LKK77 pKa = 10.91SRR79 pKa = 11.84GNFINKK85 pKa = 8.51VVTANLQSSILRR97 pKa = 11.84FQAINRR103 pKa = 11.84YY104 pKa = 6.85MNRR107 pKa = 11.84TEE109 pKa = 4.07LQRR112 pKa = 11.84NTAFTYY118 pKa = 10.28GGAYY122 pKa = 10.16SLQNVGVPTQTFCPLHH138 pKa = 7.5LYY140 pKa = 10.58DD141 pKa = 4.38ITCSPNIVNTTVTQPVVAQVLTLRR165 pKa = 11.84TGAVPFSTPTSTNFEE180 pKa = 4.33YY181 pKa = 10.69LYY183 pKa = 10.9GDD185 pKa = 3.89SLAGSDD191 pKa = 4.17GVSTGWQQEE200 pKa = 4.02QLPSGFGTVVYY211 pKa = 9.68PNRR214 pKa = 11.84RR215 pKa = 11.84TLLEE219 pKa = 4.2WVSLDD224 pKa = 3.37MFCYY228 pKa = 10.0GAILMPVTYY237 pKa = 8.93TVSVVKK243 pKa = 10.36FSKK246 pKa = 10.5DD247 pKa = 3.37YY248 pKa = 10.62LHH250 pKa = 7.25PGMGGIDD257 pKa = 3.75FNAIDD262 pKa = 4.66DD263 pKa = 4.04VRR265 pKa = 11.84LDD267 pKa = 4.38RR268 pKa = 11.84LNFWLRR274 pKa = 11.84QIKK277 pKa = 10.34SDD279 pKa = 3.21ISHH282 pKa = 7.33PLALEE287 pKa = 4.26GQTGNARR294 pKa = 11.84LKK296 pKa = 10.21VLKK299 pKa = 8.62TQSFTLQPSSNAVGEE314 pKa = 4.29GGITNPAPRR323 pKa = 11.84TKK325 pKa = 10.47RR326 pKa = 11.84FTMKK330 pKa = 10.35IAINDD335 pKa = 3.41VMKK338 pKa = 10.87YY339 pKa = 10.26DD340 pKa = 3.19WNDD343 pKa = 3.26RR344 pKa = 11.84VNTDD348 pKa = 4.29NNILDD353 pKa = 4.24PGSTIEE359 pKa = 4.53NKK361 pKa = 10.8GEE363 pKa = 3.53MSTYY367 pKa = 9.98VDD369 pKa = 3.61YY370 pKa = 11.19KK371 pKa = 9.8KK372 pKa = 10.39RR373 pKa = 11.84IYY375 pKa = 10.84IMVQATNRR383 pKa = 11.84APTEE387 pKa = 3.99NDD389 pKa = 2.9SSDD392 pKa = 2.77WRR394 pKa = 11.84FSPSYY399 pKa = 10.62DD400 pKa = 2.74IVIRR404 pKa = 11.84KK405 pKa = 9.54KK406 pKa = 9.48YY407 pKa = 9.26TNITT411 pKa = 3.44

Molecular weight: 46.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

712

301

411

356.0

40.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.601 ± 0.985

0.843 ± 0.089

5.758 ± 0.704

4.494 ± 1.626

3.792 ± 0.304

6.461 ± 0.49

1.404 ± 0.532

5.618 ± 0.401

6.32 ± 0.187

5.899 ± 0.721

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.949 ± 0.168

5.197 ± 0.891

4.213 ± 0.323

3.652 ± 0.19

8.567 ± 0.727

7.444 ± 1.23

7.163 ± 1.643

7.303 ± 0.189

1.404 ± 0.148

4.916 ± 0.615
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski