Mycobacterium phage Fowlmouth

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fowlmouthvirus; Mycobacterium virus Fowlmouth

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 120 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G2KGC4|A0A3G2KGC4_9CAUD Uncharacterized protein OS=Mycobacterium phage Fowlmouth OX=2419978 GN=65 PE=4 SV=1
MM1 pKa = 7.1TAPIVLNVLGYY12 pKa = 8.76FSPSPKK18 pKa = 9.48YY19 pKa = 9.37ANSVAMVLDD28 pKa = 4.61LTDD31 pKa = 3.1SWDD34 pKa = 3.69DD35 pKa = 3.7DD36 pKa = 4.33FGDD39 pKa = 3.44VDD41 pKa = 3.75TYY43 pKa = 9.96GTQVMVFYY51 pKa = 10.96RR52 pKa = 11.84FTIQEE57 pKa = 3.67AYY59 pKa = 10.37AAVNAYY65 pKa = 8.84IQNTTTGFNSFVPWRR80 pKa = 11.84DD81 pKa = 2.74ASQLIDD87 pKa = 5.03IITEE91 pKa = 4.02GQNWIVTTDD100 pKa = 2.62SDD102 pKa = 4.04GFVNAEE108 pKa = 4.06CFPNLEE114 pKa = 4.05EE115 pKa = 5.09AEE117 pKa = 4.35RR118 pKa = 11.84IYY120 pKa = 11.1LDD122 pKa = 3.32RR123 pKa = 11.84RR124 pKa = 11.84TDD126 pKa = 3.77FQVWAKK132 pKa = 9.77EE133 pKa = 4.11RR134 pKa = 11.84PP135 pKa = 3.5

Molecular weight: 15.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G2KGF0|A0A3G2KGF0_9CAUD Resolvase OS=Mycobacterium phage Fowlmouth OX=2419978 GN=91 PE=4 SV=1
MM1 pKa = 7.47KK2 pKa = 10.38FSDD5 pKa = 3.35IRR7 pKa = 11.84GTVFKK12 pKa = 9.65GTPEE16 pKa = 4.01DD17 pKa = 3.89EE18 pKa = 4.64YY19 pKa = 11.63NRR21 pKa = 11.84HH22 pKa = 6.25AGVATPRR29 pKa = 11.84RR30 pKa = 11.84VAPQVHH36 pKa = 6.44KK37 pKa = 11.21ASMDD41 pKa = 3.53LKK43 pKa = 11.15KK44 pKa = 10.4EE45 pKa = 4.04FKK47 pKa = 10.52DD48 pKa = 3.8AGFTRR53 pKa = 11.84ADD55 pKa = 3.79LMAPQQNRR63 pKa = 11.84EE64 pKa = 3.71YY65 pKa = 9.95RR66 pKa = 11.84RR67 pKa = 11.84AMRR70 pKa = 11.84KK71 pKa = 8.53PFGRR75 pKa = 11.84NRR77 pKa = 11.84LRR79 pKa = 11.84HH80 pKa = 4.54QLKK83 pKa = 9.52TNPHH87 pKa = 4.93WFKK90 pKa = 11.05RR91 pKa = 11.84HH92 pKa = 4.9MIEE95 pKa = 3.96VNMRR99 pKa = 11.84KK100 pKa = 9.42ALEE103 pKa = 3.67QDD105 pKa = 3.83RR106 pKa = 11.84IRR108 pKa = 11.84RR109 pKa = 11.84GLPSFDD115 pKa = 3.86DD116 pKa = 4.25AYY118 pKa = 10.84EE119 pKa = 4.12RR120 pKa = 11.84MII122 pKa = 5.06

Molecular weight: 14.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

120

0

120

21999

37

1837

183.3

20.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.923 ± 0.392

1.0 ± 0.155

6.441 ± 0.192

6.6 ± 0.269

3.964 ± 0.126

7.723 ± 0.4

1.9 ± 0.16

5.764 ± 0.215

5.673 ± 0.274

7.678 ± 0.16

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.509 ± 0.178

4.496 ± 0.206

4.977 ± 0.179

4.1 ± 0.258

5.741 ± 0.375

6.682 ± 0.241

5.532 ± 0.27

6.432 ± 0.183

1.932 ± 0.145

2.932 ± 0.176
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski