Amino acid dipepetide frequency for Escherichia phage PGN590

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.26AlaAla: 7.26 ± 0.853
0.844AlaCys: 0.844 ± 0.304
4.052AlaAsp: 4.052 ± 0.858
5.403AlaGlu: 5.403 ± 0.795
2.955AlaPhe: 2.955 ± 0.534
5.234AlaGly: 5.234 ± 0.637
0.844AlaHis: 0.844 ± 0.281
6.247AlaIle: 6.247 ± 0.944
7.007AlaLys: 7.007 ± 0.984
6.585AlaLeu: 6.585 ± 0.707
2.448AlaMet: 2.448 ± 0.442
4.136AlaAsn: 4.136 ± 0.727
1.604AlaPro: 1.604 ± 0.317
3.714AlaGln: 3.714 ± 0.638
5.403AlaArg: 5.403 ± 0.692
5.74AlaSer: 5.74 ± 0.761
4.643AlaThr: 4.643 ± 0.771
5.825AlaVal: 5.825 ± 0.838
1.351AlaTrp: 1.351 ± 0.335
2.279AlaTyr: 2.279 ± 0.484
0.0AlaXaa: 0.0 ± 0.0
Cys
1.097CysAla: 1.097 ± 0.307
0.169CysCys: 0.169 ± 0.133
1.351CysAsp: 1.351 ± 0.304
0.844CysGlu: 0.844 ± 0.279
0.675CysPhe: 0.675 ± 0.308
1.351CysGly: 1.351 ± 0.422
0.422CysHis: 0.422 ± 0.2
0.675CysIle: 0.675 ± 0.226
1.266CysLys: 1.266 ± 0.395
1.013CysLeu: 1.013 ± 0.334
0.591CysMet: 0.591 ± 0.209
0.76CysAsn: 0.76 ± 0.285
0.422CysPro: 0.422 ± 0.191
0.253CysGln: 0.253 ± 0.149
0.591CysArg: 0.591 ± 0.296
0.675CysSer: 0.675 ± 0.292
0.844CysThr: 0.844 ± 0.297
1.097CysVal: 1.097 ± 0.277
0.338CysTrp: 0.338 ± 0.148
0.253CysTyr: 0.253 ± 0.163
0.0CysXaa: 0.0 ± 0.0
Asp
5.149AspAla: 5.149 ± 0.648
0.422AspCys: 0.422 ± 0.188
3.377AspAsp: 3.377 ± 0.53
4.305AspGlu: 4.305 ± 0.5
1.942AspPhe: 1.942 ± 0.369
5.487AspGly: 5.487 ± 0.881
1.013AspHis: 1.013 ± 0.259
2.364AspIle: 2.364 ± 0.353
4.221AspLys: 4.221 ± 0.51
4.052AspLeu: 4.052 ± 0.582
1.773AspMet: 1.773 ± 0.429
2.364AspAsn: 2.364 ± 0.456
2.026AspPro: 2.026 ± 0.407
2.026AspGln: 2.026 ± 0.362
2.195AspArg: 2.195 ± 0.416
3.292AspSer: 3.292 ± 0.655
3.377AspThr: 3.377 ± 0.492
2.955AspVal: 2.955 ± 0.458
1.097AspTrp: 1.097 ± 0.284
2.786AspTyr: 2.786 ± 0.466
0.0AspXaa: 0.0 ± 0.0
Glu
5.403GluAla: 5.403 ± 0.857
0.591GluCys: 0.591 ± 0.238
2.87GluAsp: 2.87 ± 0.456
3.546GluGlu: 3.546 ± 0.491
3.039GluPhe: 3.039 ± 0.515
2.955GluGly: 2.955 ± 0.388
1.182GluHis: 1.182 ± 0.276
5.825GluIle: 5.825 ± 0.632
4.643GluLys: 4.643 ± 0.612
5.149GluLeu: 5.149 ± 0.715
2.364GluMet: 2.364 ± 0.392
2.955GluAsn: 2.955 ± 0.494
1.351GluPro: 1.351 ± 0.424
2.955GluGln: 2.955 ± 0.51
3.039GluArg: 3.039 ± 0.688
4.136GluSer: 4.136 ± 0.511
3.714GluThr: 3.714 ± 0.693
4.221GluVal: 4.221 ± 0.609
0.76GluTrp: 0.76 ± 0.35
2.533GluTyr: 2.533 ± 0.525
0.0GluXaa: 0.0 ± 0.0
Phe
2.533PheAla: 2.533 ± 0.413
0.507PheCys: 0.507 ± 0.188
2.533PheAsp: 2.533 ± 0.59
2.11PheGlu: 2.11 ± 0.4
1.688PhePhe: 1.688 ± 0.517
2.364PheGly: 2.364 ± 0.553
0.422PheHis: 0.422 ± 0.182
3.292PheIle: 3.292 ± 0.628
2.955PheLys: 2.955 ± 0.375
2.448PheLeu: 2.448 ± 0.53
1.013PheMet: 1.013 ± 0.303
2.364PheAsn: 2.364 ± 0.347
1.604PhePro: 1.604 ± 0.446
1.773PheGln: 1.773 ± 0.408
2.364PheArg: 2.364 ± 0.426
2.364PheSer: 2.364 ± 0.413
2.955PheThr: 2.955 ± 0.503
2.533PheVal: 2.533 ± 0.498
0.591PheTrp: 0.591 ± 0.235
1.013PheTyr: 1.013 ± 0.345
0.0PheXaa: 0.0 ± 0.0
Gly
4.305GlyAla: 4.305 ± 0.864
1.182GlyCys: 1.182 ± 0.307
4.052GlyAsp: 4.052 ± 0.599
5.318GlyGlu: 5.318 ± 0.734
2.533GlyPhe: 2.533 ± 0.441
6.838GlyGly: 6.838 ± 1.346
1.097GlyHis: 1.097 ± 0.39
4.727GlyIle: 4.727 ± 0.693
6.162GlyLys: 6.162 ± 0.64
4.981GlyLeu: 4.981 ± 0.55
1.942GlyMet: 1.942 ± 0.369
3.377GlyAsn: 3.377 ± 0.619
0.591GlyPro: 0.591 ± 0.268
2.279GlyGln: 2.279 ± 0.403
2.701GlyArg: 2.701 ± 0.355
6.331GlySer: 6.331 ± 0.733
2.701GlyThr: 2.701 ± 0.466
5.825GlyVal: 5.825 ± 0.692
1.351GlyTrp: 1.351 ± 0.345
3.546GlyTyr: 3.546 ± 0.515
0.0GlyXaa: 0.0 ± 0.0
His
0.338HisAla: 0.338 ± 0.165
0.253HisCys: 0.253 ± 0.192
1.097HisAsp: 1.097 ± 0.337
0.844HisGlu: 0.844 ± 0.342
1.182HisPhe: 1.182 ± 0.417
1.773HisGly: 1.773 ± 0.476
0.253HisHis: 0.253 ± 0.275
1.604HisIle: 1.604 ± 0.411
1.435HisLys: 1.435 ± 0.346
1.688HisLeu: 1.688 ± 0.4
0.253HisMet: 0.253 ± 0.183
1.013HisAsn: 1.013 ± 0.385
0.591HisPro: 0.591 ± 0.285
0.675HisGln: 0.675 ± 0.295
1.013HisArg: 1.013 ± 0.291
0.929HisSer: 0.929 ± 0.295
0.675HisThr: 0.675 ± 0.306
1.351HisVal: 1.351 ± 0.358
0.338HisTrp: 0.338 ± 0.156
1.182HisTyr: 1.182 ± 0.316
0.0HisXaa: 0.0 ± 0.0
Ile
6.162IleAla: 6.162 ± 0.696
1.688IleCys: 1.688 ± 0.456
4.559IleAsp: 4.559 ± 0.685
4.474IleGlu: 4.474 ± 0.515
2.11IlePhe: 2.11 ± 0.491
4.305IleGly: 4.305 ± 0.507
1.52IleHis: 1.52 ± 0.364
4.136IleIle: 4.136 ± 0.642
5.403IleLys: 5.403 ± 0.759
3.292IleLeu: 3.292 ± 0.44
1.942IleMet: 1.942 ± 0.454
3.292IleAsn: 3.292 ± 0.579
2.701IlePro: 2.701 ± 0.503
2.955IleGln: 2.955 ± 0.627
2.955IleArg: 2.955 ± 0.525
4.136IleSer: 4.136 ± 0.631
5.656IleThr: 5.656 ± 0.783
4.221IleVal: 4.221 ± 0.689
1.013IleTrp: 1.013 ± 0.344
2.195IleTyr: 2.195 ± 0.407
0.0IleXaa: 0.0 ± 0.0
Lys
6.585LysAla: 6.585 ± 1.213
0.929LysCys: 0.929 ± 0.296
4.896LysAsp: 4.896 ± 0.609
5.74LysGlu: 5.74 ± 0.674
2.448LysPhe: 2.448 ± 0.465
4.39LysGly: 4.39 ± 0.631
1.604LysHis: 1.604 ± 0.414
4.052LysIle: 4.052 ± 0.694
3.968LysLys: 3.968 ± 0.704
5.065LysLeu: 5.065 ± 0.666
2.533LysMet: 2.533 ± 0.496
3.377LysAsn: 3.377 ± 0.641
3.714LysPro: 3.714 ± 0.603
3.714LysGln: 3.714 ± 0.655
4.896LysArg: 4.896 ± 0.718
3.799LysSer: 3.799 ± 0.562
4.305LysThr: 4.305 ± 0.561
4.643LysVal: 4.643 ± 0.475
1.013LysTrp: 1.013 ± 0.289
1.351LysTyr: 1.351 ± 0.32
0.0LysXaa: 0.0 ± 0.0
Leu
6.247LeuAla: 6.247 ± 0.773
1.097LeuCys: 1.097 ± 0.375
2.955LeuAsp: 2.955 ± 0.485
3.883LeuGlu: 3.883 ± 0.624
2.617LeuPhe: 2.617 ± 0.684
3.63LeuGly: 3.63 ± 0.78
1.351LeuHis: 1.351 ± 0.452
4.896LeuIle: 4.896 ± 0.631
5.909LeuLys: 5.909 ± 0.764
5.065LeuLeu: 5.065 ± 0.762
2.364LeuMet: 2.364 ± 0.457
3.461LeuAsn: 3.461 ± 0.616
3.799LeuPro: 3.799 ± 0.589
1.857LeuGln: 1.857 ± 0.407
3.546LeuArg: 3.546 ± 0.567
4.896LeuSer: 4.896 ± 0.635
4.39LeuThr: 4.39 ± 0.544
3.292LeuVal: 3.292 ± 0.412
1.097LeuTrp: 1.097 ± 0.293
2.617LeuTyr: 2.617 ± 0.42
0.0LeuXaa: 0.0 ± 0.0
Met
2.87MetAla: 2.87 ± 0.493
0.338MetCys: 0.338 ± 0.166
1.351MetAsp: 1.351 ± 0.296
1.351MetGlu: 1.351 ± 0.402
0.929MetPhe: 0.929 ± 0.318
0.76MetGly: 0.76 ± 0.227
0.844MetHis: 0.844 ± 0.292
3.039MetIle: 3.039 ± 0.503
2.195MetLys: 2.195 ± 0.417
2.026MetLeu: 2.026 ± 0.445
1.182MetMet: 1.182 ± 0.357
1.351MetAsn: 1.351 ± 0.39
1.52MetPro: 1.52 ± 0.522
0.929MetGln: 0.929 ± 0.259
1.942MetArg: 1.942 ± 0.322
2.11MetSer: 2.11 ± 0.383
1.688MetThr: 1.688 ± 0.391
1.52MetVal: 1.52 ± 0.348
0.084MetTrp: 0.084 ± 0.088
0.844MetTyr: 0.844 ± 0.239
0.0MetXaa: 0.0 ± 0.0
Asn
4.896AsnAla: 4.896 ± 0.586
0.422AsnCys: 0.422 ± 0.183
2.617AsnAsp: 2.617 ± 0.591
2.87AsnGlu: 2.87 ± 0.422
1.857AsnPhe: 1.857 ± 0.327
5.318AsnGly: 5.318 ± 0.756
1.013AsnHis: 1.013 ± 0.293
3.461AsnIle: 3.461 ± 0.458
3.377AsnLys: 3.377 ± 0.567
2.448AsnLeu: 2.448 ± 0.451
0.929AsnMet: 0.929 ± 0.267
2.533AsnAsn: 2.533 ± 0.509
2.11AsnPro: 2.11 ± 0.392
1.688AsnGln: 1.688 ± 0.438
1.857AsnArg: 1.857 ± 0.402
2.87AsnSer: 2.87 ± 0.522
1.604AsnThr: 1.604 ± 0.332
3.208AsnVal: 3.208 ± 0.458
0.844AsnTrp: 0.844 ± 0.232
1.604AsnTyr: 1.604 ± 0.402
0.0AsnXaa: 0.0 ± 0.0
Pro
3.039ProAla: 3.039 ± 0.656
0.76ProCys: 0.76 ± 0.263
2.701ProAsp: 2.701 ± 0.634
3.208ProGlu: 3.208 ± 0.581
1.604ProPhe: 1.604 ± 0.371
2.701ProGly: 2.701 ± 0.553
0.929ProHis: 0.929 ± 0.229
2.448ProIle: 2.448 ± 0.603
1.52ProLys: 1.52 ± 0.302
2.026ProLeu: 2.026 ± 0.39
1.351ProMet: 1.351 ± 0.278
1.857ProAsn: 1.857 ± 0.459
1.604ProPro: 1.604 ± 0.407
1.435ProGln: 1.435 ± 0.378
1.688ProArg: 1.688 ± 0.44
2.11ProSer: 2.11 ± 0.401
1.435ProThr: 1.435 ± 0.301
2.786ProVal: 2.786 ± 0.504
0.422ProTrp: 0.422 ± 0.189
1.351ProTyr: 1.351 ± 0.358
0.0ProXaa: 0.0 ± 0.0
Gln
3.123GlnAla: 3.123 ± 0.526
0.675GlnCys: 0.675 ± 0.233
1.857GlnAsp: 1.857 ± 0.347
2.026GlnGlu: 2.026 ± 0.392
1.097GlnPhe: 1.097 ± 0.299
2.195GlnGly: 2.195 ± 0.37
0.844GlnHis: 0.844 ± 0.278
3.123GlnIle: 3.123 ± 0.615
2.279GlnLys: 2.279 ± 0.454
2.87GlnLeu: 2.87 ± 0.633
0.929GlnMet: 0.929 ± 0.32
1.604GlnAsn: 1.604 ± 0.329
1.604GlnPro: 1.604 ± 0.558
3.039GlnGln: 3.039 ± 0.913
2.364GlnArg: 2.364 ± 0.407
2.955GlnSer: 2.955 ± 0.46
1.604GlnThr: 1.604 ± 0.372
2.955GlnVal: 2.955 ± 0.5
0.591GlnTrp: 0.591 ± 0.265
1.688GlnTyr: 1.688 ± 0.358
0.0GlnXaa: 0.0 ± 0.0
Arg
4.981ArgAla: 4.981 ± 0.605
0.929ArgCys: 0.929 ± 0.395
2.026ArgAsp: 2.026 ± 0.452
2.701ArgGlu: 2.701 ± 0.406
2.701ArgPhe: 2.701 ± 0.519
3.292ArgGly: 3.292 ± 0.383
0.507ArgHis: 0.507 ± 0.206
3.461ArgIle: 3.461 ± 0.494
4.39ArgLys: 4.39 ± 0.553
3.377ArgLeu: 3.377 ± 0.708
2.026ArgMet: 2.026 ± 0.412
2.448ArgAsn: 2.448 ± 0.503
1.942ArgPro: 1.942 ± 0.49
2.026ArgGln: 2.026 ± 0.483
3.799ArgArg: 3.799 ± 0.592
3.123ArgSer: 3.123 ± 0.543
1.773ArgThr: 1.773 ± 0.368
3.968ArgVal: 3.968 ± 0.617
0.591ArgTrp: 0.591 ± 0.248
2.448ArgTyr: 2.448 ± 0.478
0.0ArgXaa: 0.0 ± 0.0
Ser
5.909SerAla: 5.909 ± 0.693
1.013SerCys: 1.013 ± 0.368
3.63SerAsp: 3.63 ± 0.475
4.812SerGlu: 4.812 ± 0.619
3.123SerPhe: 3.123 ± 0.481
5.234SerGly: 5.234 ± 0.723
1.351SerHis: 1.351 ± 0.304
4.136SerIle: 4.136 ± 0.551
4.474SerLys: 4.474 ± 0.58
5.234SerLeu: 5.234 ± 0.681
0.844SerMet: 0.844 ± 0.212
2.279SerAsn: 2.279 ± 0.402
3.039SerPro: 3.039 ± 0.464
2.364SerGln: 2.364 ± 0.445
3.123SerArg: 3.123 ± 0.507
4.727SerSer: 4.727 ± 1.046
3.714SerThr: 3.714 ± 0.698
4.559SerVal: 4.559 ± 0.58
0.507SerTrp: 0.507 ± 0.201
2.448SerTyr: 2.448 ± 0.533
0.0SerXaa: 0.0 ± 0.0
Thr
4.812ThrAla: 4.812 ± 0.641
1.097ThrCys: 1.097 ± 0.341
3.208ThrAsp: 3.208 ± 0.563
2.533ThrGlu: 2.533 ± 0.455
2.448ThrPhe: 2.448 ± 0.516
6.162ThrGly: 6.162 ± 0.893
0.507ThrHis: 0.507 ± 0.175
3.799ThrIle: 3.799 ± 0.675
3.546ThrLys: 3.546 ± 0.734
4.136ThrLeu: 4.136 ± 0.445
1.182ThrMet: 1.182 ± 0.368
3.292ThrAsn: 3.292 ± 0.462
2.364ThrPro: 2.364 ± 0.429
2.026ThrGln: 2.026 ± 0.384
1.857ThrArg: 1.857 ± 0.385
4.221ThrSer: 4.221 ± 0.645
2.279ThrThr: 2.279 ± 0.438
3.377ThrVal: 3.377 ± 0.404
0.591ThrTrp: 0.591 ± 0.214
1.773ThrTyr: 1.773 ± 0.4
0.0ThrXaa: 0.0 ± 0.0
Val
4.981ValAla: 4.981 ± 0.618
1.097ValCys: 1.097 ± 0.3
4.474ValAsp: 4.474 ± 0.451
4.052ValGlu: 4.052 ± 0.564
2.533ValPhe: 2.533 ± 0.573
4.052ValGly: 4.052 ± 0.697
1.52ValHis: 1.52 ± 0.36
4.727ValIle: 4.727 ± 0.547
4.727ValLys: 4.727 ± 0.668
4.727ValLeu: 4.727 ± 0.481
1.688ValMet: 1.688 ± 0.398
3.292ValAsn: 3.292 ± 0.483
2.026ValPro: 2.026 ± 0.688
1.773ValGln: 1.773 ± 0.396
3.63ValArg: 3.63 ± 0.531
4.896ValSer: 4.896 ± 0.706
4.221ValThr: 4.221 ± 0.565
4.39ValVal: 4.39 ± 0.506
0.591ValTrp: 0.591 ± 0.207
1.857ValTyr: 1.857 ± 0.395
0.0ValXaa: 0.0 ± 0.0
Trp
0.76TrpAla: 0.76 ± 0.343
0.338TrpCys: 0.338 ± 0.235
0.675TrpAsp: 0.675 ± 0.215
0.675TrpGlu: 0.675 ± 0.209
0.76TrpPhe: 0.76 ± 0.342
1.266TrpGly: 1.266 ± 0.354
0.591TrpHis: 0.591 ± 0.235
1.182TrpIle: 1.182 ± 0.279
0.76TrpLys: 0.76 ± 0.218
1.013TrpLeu: 1.013 ± 0.293
0.422TrpMet: 0.422 ± 0.178
0.253TrpAsn: 0.253 ± 0.18
0.253TrpPro: 0.253 ± 0.178
0.422TrpGln: 0.422 ± 0.171
1.52TrpArg: 1.52 ± 0.389
0.844TrpSer: 0.844 ± 0.3
0.76TrpThr: 0.76 ± 0.248
0.422TrpVal: 0.422 ± 0.158
0.169TrpTrp: 0.169 ± 0.101
0.591TrpTyr: 0.591 ± 0.234
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.123TyrAla: 3.123 ± 0.618
0.338TyrCys: 0.338 ± 0.17
1.942TyrAsp: 1.942 ± 0.376
2.533TyrGlu: 2.533 ± 0.434
1.182TyrPhe: 1.182 ± 0.302
2.533TyrGly: 2.533 ± 0.439
0.675TyrHis: 0.675 ± 0.276
1.52TyrIle: 1.52 ± 0.341
2.87TyrLys: 2.87 ± 0.482
1.773TyrLeu: 1.773 ± 0.418
1.013TyrMet: 1.013 ± 0.254
1.52TyrAsn: 1.52 ± 0.36
2.11TyrPro: 2.11 ± 0.495
1.52TyrGln: 1.52 ± 0.259
2.026TyrArg: 2.026 ± 0.444
2.364TyrSer: 2.364 ± 0.501
2.87TyrThr: 2.87 ± 0.459
2.11TyrVal: 2.11 ± 0.298
0.338TyrTrp: 0.338 ± 0.148
1.52TyrTyr: 1.52 ± 0.312
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 51 proteins (11847 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski