Arsenicibacter rosenii
Average proteome isoelectric point is 6.84
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5902 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1S2VHF7|A0A1S2VHF7_9BACT Uncharacterized protein OS=Arsenicibacter rosenii OX=1750698 GN=BLX24_18155 PE=4 SV=1
MM1 pKa = 7.11 KK2 pKa = 9.87 TLTNFLADD10 pKa = 3.81 EE11 pKa = 5.56 AILSPAEE18 pKa = 3.74 AKK20 pKa = 10.75 AIVGGSAIYY29 pKa = 10.86 YY30 pKa = 10.04 NGILVATTDD39 pKa = 3.94 DD40 pKa = 3.77 EE41 pKa = 4.93 TGVTTVHH48 pKa = 5.83 NQALYY53 pKa = 10.8 DD54 pKa = 4.29 LYY56 pKa = 11.06 LANPSIQGFGSMLSVLDD73 pKa = 3.58 TDD75 pKa = 4.76 FF76 pKa = 5.55
Molecular weight: 8.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.768
IPC2_protein 3.872
IPC_protein 3.745
Toseland 3.554
ProMoST 3.961
Dawson 3.77
Bjellqvist 3.935
Wikipedia 3.745
Rodwell 3.592
Grimsley 3.478
Solomon 3.732
Lehninger 3.681
Nozaki 3.91
DTASelect 4.113
Thurlkill 3.643
EMBOSS 3.745
Sillero 3.884
Patrickios 0.604
IPC_peptide 3.719
IPC2_peptide 3.846
IPC2.peptide.svr19 3.799
Protein with the highest isoelectric point:
>tr|A0A1S2VCL0|A0A1S2VCL0_9BACT Ribosome-recycling factor OS=Arsenicibacter rosenii OX=1750698 GN=frr PE=3 SV=1
MM1 pKa = 7.66 ACLKK5 pKa = 10.22 RR6 pKa = 11.84 LRR8 pKa = 11.84 AVAPAVEE15 pKa = 4.3 RR16 pKa = 11.84 EE17 pKa = 4.13 VVYY20 pKa = 10.75 AGYY23 pKa = 10.67 AFEE26 pKa = 4.48 EE27 pKa = 4.78 SMVAQEE33 pKa = 3.91 FRR35 pKa = 11.84 AAARR39 pKa = 11.84 GDD41 pKa = 4.05 LNSQHH46 pKa = 5.33 TVQRR50 pKa = 11.84 EE51 pKa = 3.74 LRR53 pKa = 11.84 EE54 pKa = 3.92 RR55 pKa = 11.84 LQQRR59 pKa = 11.84 GRR61 pKa = 11.84 LFKK64 pKa = 9.82 NTAGFIVSNPLHH76 pKa = 6.17 SAISTLKK83 pKa = 10.46 RR84 pKa = 11.84 SLII87 pKa = 4.04
Molecular weight: 9.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.345
IPC2_protein 9.56
IPC_protein 10.526
Toseland 10.613
ProMoST 10.379
Dawson 10.716
Bjellqvist 10.467
Wikipedia 10.95
Rodwell 10.804
Grimsley 10.774
Solomon 10.862
Lehninger 10.833
Nozaki 10.599
DTASelect 10.452
Thurlkill 10.613
EMBOSS 11.023
Sillero 10.657
Patrickios 10.599
IPC_peptide 10.862
IPC2_peptide 9.575
IPC2.peptide.svr19 8.589
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5902
0
5902
2137068
26
3503
362.1
40.28
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.54 ± 0.033
0.803 ± 0.012
5.289 ± 0.025
5.113 ± 0.035
4.462 ± 0.022
7.39 ± 0.036
1.833 ± 0.018
5.847 ± 0.026
5.033 ± 0.035
9.78 ± 0.04
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.209 ± 0.015
4.653 ± 0.03
4.636 ± 0.021
4.38 ± 0.025
5.3 ± 0.033
6.049 ± 0.035
6.604 ± 0.054
6.864 ± 0.03
1.345 ± 0.014
3.867 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here