Papillibacter cinnamivorans DSM 12816
Average proteome isoelectric point is 6.3
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2640 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1W1YI55|A0A1W1YI55_9FIRM Purine-binding chemotaxis protein CheW OS=Papillibacter cinnamivorans DSM 12816 OX=1122930 GN=SAMN02745168_0438 PE=4 SV=1
MM1 pKa = 7.67 SSTFLGIQIGKK12 pKa = 9.71 SGLNAAQINLNITGQNISNADD33 pKa = 3.5 TQGYY37 pKa = 6.33 TRR39 pKa = 11.84 QSVITSSAPPSGAGYY54 pKa = 9.97 VIRR57 pKa = 11.84 QVTQSSNVGQGVRR70 pKa = 11.84 VLSVDD75 pKa = 3.6 QLRR78 pKa = 11.84 SAYY81 pKa = 10.48 LDD83 pKa = 3.35 EE84 pKa = 4.84 QYY86 pKa = 10.96 RR87 pKa = 11.84 SQYY90 pKa = 11.38 SDD92 pKa = 3.27 FCSSEE97 pKa = 3.51 YY98 pKa = 9.38 RR99 pKa = 11.84 TQGLSYY105 pKa = 11.06 LEE107 pKa = 4.71 DD108 pKa = 4.3 LFDD111 pKa = 4.42 EE112 pKa = 5.49 LDD114 pKa = 4.31 DD115 pKa = 4.16 NTSLTASISDD125 pKa = 3.84 FFDD128 pKa = 3.69 ALSDD132 pKa = 3.86 FAGDD136 pKa = 3.78 TTSEE140 pKa = 4.12 AARR143 pKa = 11.84 TTVQQTARR151 pKa = 11.84 SMTDD155 pKa = 3.24 NFNMIYY161 pKa = 10.89 GEE163 pKa = 4.49 MVDD166 pKa = 5.57 LYY168 pKa = 11.01 NDD170 pKa = 3.18 QNTSVRR176 pKa = 11.84 TVAEE180 pKa = 4.29 QISSLASQIADD191 pKa = 3.92 LNATICDD198 pKa = 3.88 YY199 pKa = 10.99 EE200 pKa = 4.92 RR201 pKa = 11.84 SGVTANDD208 pKa = 3.65 LRR210 pKa = 11.84 DD211 pKa = 3.68 QRR213 pKa = 11.84 NLLLDD218 pKa = 3.84 KK219 pKa = 11.13 LSGYY223 pKa = 11.17 SDD225 pKa = 3.92 FTCSEE230 pKa = 4.27 DD231 pKa = 3.24 AKK233 pKa = 11.35 GMVNVWIAGEE243 pKa = 3.94 ALVDD247 pKa = 3.89 GKK249 pKa = 8.79 TAGSISITSAADD261 pKa = 4.06 EE262 pKa = 4.81 IDD264 pKa = 3.75 TLCQQLSILNGGVLSAGIITPDD286 pKa = 3.87 QEE288 pKa = 4.26 TQRR291 pKa = 11.84 DD292 pKa = 4.93 GICSALQQISGKK304 pKa = 10.02 ISCNVNADD312 pKa = 3.21 GTASVSIDD320 pKa = 3.53 YY321 pKa = 10.68 VSASMTQVTDD331 pKa = 3.57 SLVDD335 pKa = 3.86 GSTCGSVSEE344 pKa = 4.37 EE345 pKa = 3.66 AVKK348 pKa = 10.94 AFDD351 pKa = 3.87 GADD354 pKa = 3.36 MEE356 pKa = 5.12 SVLKK360 pKa = 11.0 LGEE363 pKa = 4.44 TYY365 pKa = 11.09 LNTDD369 pKa = 3.53 TVSGGEE375 pKa = 4.07 LFAHH379 pKa = 6.95 LSLRR383 pKa = 11.84 GGDD386 pKa = 3.54 TSGDD390 pKa = 3.28 AGVPYY395 pKa = 10.76 YY396 pKa = 10.0 IGRR399 pKa = 11.84 LDD401 pKa = 3.73 DD402 pKa = 3.83 LARR405 pKa = 11.84 TVAEE409 pKa = 4.28 TVNEE413 pKa = 4.13 CMNKK417 pKa = 9.88 GYY419 pKa = 9.1 TYY421 pKa = 10.34 PDD423 pKa = 3.55 EE424 pKa = 4.81 EE425 pKa = 4.74 NGFSSVTGTDD435 pKa = 2.52 VDD437 pKa = 4.33 LFADD441 pKa = 4.96 FGNQYY446 pKa = 11.74 ALVTAGNFSVSDD458 pKa = 3.9 SVLSSVWNIAGSDD471 pKa = 3.91 SEE473 pKa = 4.96 IDD475 pKa = 3.67 LSTDD479 pKa = 2.97 STQTSNNKK487 pKa = 8.95 VALLLADD494 pKa = 5.62 LINNTDD500 pKa = 3.72 YY501 pKa = 11.96 GDD503 pKa = 3.87 MLDD506 pKa = 4.52 GLISHH511 pKa = 7.69 LGQTVSGSQSLLDD524 pKa = 3.58 TRR526 pKa = 11.84 EE527 pKa = 4.01 SLVEE531 pKa = 3.78 STEE534 pKa = 3.86 NQRR537 pKa = 11.84 QSISGVSVDD546 pKa = 3.97 EE547 pKa = 4.3 EE548 pKa = 4.33 AVNLIMYY555 pKa = 7.22 QQTYY559 pKa = 8.23 NACSRR564 pKa = 11.84 VITTMDD570 pKa = 3.01 QMLDD574 pKa = 3.22 KK575 pKa = 10.66 LINGTGTVGLL585 pKa = 4.32
Molecular weight: 62.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.719
IPC2_protein 3.783
IPC_protein 3.821
Toseland 3.592
ProMoST 3.986
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.757
Rodwell 3.643
Grimsley 3.503
Solomon 3.821
Lehninger 3.77
Nozaki 3.923
DTASelect 4.19
Thurlkill 3.643
EMBOSS 3.77
Sillero 3.948
Patrickios 1.888
IPC_peptide 3.808
IPC2_peptide 3.923
IPC2.peptide.svr19 3.843
Protein with the highest isoelectric point:
>tr|A0A1W1Z0F6|A0A1W1Z0F6_9FIRM Stage II sporulation protein R OS=Papillibacter cinnamivorans DSM 12816 OX=1122930 GN=SAMN02745168_0836 PE=4 SV=1
MM1 pKa = 7.74 WLYY4 pKa = 11.28 KK5 pKa = 10.34 LGRR8 pKa = 11.84 SFRR11 pKa = 11.84 YY12 pKa = 9.17 AGRR15 pKa = 11.84 GFAHH19 pKa = 6.11 VLRR22 pKa = 11.84 RR23 pKa = 11.84 EE24 pKa = 3.98 RR25 pKa = 11.84 NLRR28 pKa = 11.84 IHH30 pKa = 6.36 LTAVFYY36 pKa = 10.16 VSWAGILGRR45 pKa = 11.84 LGGAEE50 pKa = 3.97 WALLWICFAMVIGAEE65 pKa = 4.15 LFNTAIEE72 pKa = 4.31 TLGNAVTRR80 pKa = 11.84 EE81 pKa = 4.02 KK82 pKa = 11.33 NEE84 pKa = 4.17 TVGKK88 pKa = 10.29 AKK90 pKa = 10.46 DD91 pKa = 3.59 VAAGAVLVCAVGSVGAAAGIFGCTGAWKK119 pKa = 8.72 TVLRR123 pKa = 11.84 SLGSSTPLSVIAIATVPAAILFIRR147 pKa = 11.84 GRR149 pKa = 11.84 GTWKK153 pKa = 10.6
Molecular weight: 16.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.35
IPC2_protein 9.677
IPC_protein 10.438
Toseland 10.657
ProMoST 10.423
Dawson 10.76
Bjellqvist 10.482
Wikipedia 10.965
Rodwell 10.994
Grimsley 10.818
Solomon 10.877
Lehninger 10.847
Nozaki 10.672
DTASelect 10.467
Thurlkill 10.657
EMBOSS 11.067
Sillero 10.701
Patrickios 10.76
IPC_peptide 10.877
IPC2_peptide 9.633
IPC2.peptide.svr19 8.55
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2640
0
2640
814393
29
4547
308.5
33.95
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.244 ± 0.049
1.421 ± 0.02
5.24 ± 0.031
6.855 ± 0.066
4.005 ± 0.038
8.224 ± 0.051
1.552 ± 0.018
6.286 ± 0.047
5.067 ± 0.045
9.893 ± 0.058
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.68 ± 0.025
3.43 ± 0.029
4.119 ± 0.032
2.72 ± 0.03
5.735 ± 0.063
6.45 ± 0.054
5.548 ± 0.067
7.126 ± 0.047
0.89 ± 0.018
3.514 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here