Fusarium asiaticum victorivirus 1
Average proteome isoelectric point is 7.23
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A386HVJ6|A0A386HVJ6_9VIRU RNA-directed RNA polymerase OS=Fusarium asiaticum victorivirus 1 OX=2268743 PE=3 SV=1
MM1 pKa = 7.52 AALVRR6 pKa = 11.84 NNFLAAVLANARR18 pKa = 11.84 GRR20 pKa = 11.84 HH21 pKa = 5.63 IGATDD26 pKa = 2.97 HH27 pKa = 6.47 RR28 pKa = 11.84 RR29 pKa = 11.84 YY30 pKa = 8.26 RR31 pKa = 11.84 TVMRR35 pKa = 11.84 TTSKK39 pKa = 10.35 IGGVNDD45 pKa = 3.46 SRR47 pKa = 11.84 SASIFYY53 pKa = 10.41 EE54 pKa = 3.49 IGRR57 pKa = 11.84 GVNSIEE63 pKa = 4.08 AALATPVDD71 pKa = 4.99 AIPRR75 pKa = 11.84 VEE77 pKa = 4.15 AAYY80 pKa = 7.4 PTNTVLAEE88 pKa = 4.09 DD89 pKa = 5.26 FIGLAKK95 pKa = 10.22 KK96 pKa = 10.41 YY97 pKa = 10.75 SNFASTFEE105 pKa = 4.1 YY106 pKa = 10.97 SSLAALAEE114 pKa = 4.03 RR115 pKa = 11.84 LARR118 pKa = 11.84 GLAAHH123 pKa = 6.49 SVFEE127 pKa = 4.49 EE128 pKa = 4.47 DD129 pKa = 2.92 WDD131 pKa = 4.32 STFLTGGRR139 pKa = 11.84 AIVVHH144 pKa = 6.9 ALGTYY149 pKa = 9.6 DD150 pKa = 4.07 GPVNSMTDD158 pKa = 3.1 TVFIPRR164 pKa = 11.84 LVNTNISGDD173 pKa = 3.67 VFGVLVAAAAGEE185 pKa = 4.38 GSAVATDD192 pKa = 3.82 VLEE195 pKa = 4.22 VDD197 pKa = 3.32 AVTRR201 pKa = 11.84 QAVIPVVGHH210 pKa = 5.47 TALPFALVDD219 pKa = 3.51 ALRR222 pKa = 11.84 VVGGNMIASDD232 pKa = 3.98 QGPLFALAVTRR243 pKa = 11.84 GIHH246 pKa = 5.82 RR247 pKa = 11.84 VLSVVGHH254 pKa = 5.37 TDD256 pKa = 2.51 EE257 pKa = 5.39 GGTVRR262 pKa = 11.84 DD263 pKa = 4.38 LLRR266 pKa = 11.84 CAAFASPFGGIHH278 pKa = 7.11 CSLNDD283 pKa = 3.75 YY284 pKa = 11.03 AGLPALSTNGVSVVSSYY301 pKa = 11.74 VDD303 pKa = 4.69 GIALTTAALVAHH315 pKa = 6.98 SDD317 pKa = 3.67 PGNEE321 pKa = 4.21 YY322 pKa = 10.03 QGEE325 pKa = 4.29 WFPTFYY331 pKa = 10.95 LGAPDD336 pKa = 3.36 SAGDD340 pKa = 3.73 KK341 pKa = 10.75 RR342 pKa = 11.84 PGEE345 pKa = 4.25 HH346 pKa = 6.37 TEE348 pKa = 3.96 GTAAMAGAIRR358 pKa = 11.84 AQILADD364 pKa = 3.31 QGRR367 pKa = 11.84 FWEE370 pKa = 4.65 NYY372 pKa = 9.09 ARR374 pKa = 11.84 GLGLIFCAEE383 pKa = 4.46 GSTSTAVRR391 pKa = 11.84 FATTASYY398 pKa = 11.57 NLGGDD403 pKa = 3.79 PRR405 pKa = 11.84 HH406 pKa = 6.08 LRR408 pKa = 11.84 YY409 pKa = 10.12 ASVAPWFWIEE419 pKa = 4.01 PTSLLPAYY427 pKa = 10.18 FLGTEE432 pKa = 4.2 AEE434 pKa = 4.75 TVGHH438 pKa = 6.75 ASFGGRR444 pKa = 11.84 DD445 pKa = 3.64 TQSQLPGWEE454 pKa = 4.4 AIVPAGGRR462 pKa = 11.84 DD463 pKa = 3.6 TTFSAYY469 pKa = 9.62 HH470 pKa = 5.43 VRR472 pKa = 11.84 LPSARR477 pKa = 11.84 RR478 pKa = 11.84 AYY480 pKa = 9.77 MLLHH484 pKa = 6.65 WLAHH488 pKa = 5.89 PLNGLGALSVRR499 pKa = 11.84 QLDD502 pKa = 3.73 PTAIIHH508 pKa = 6.55 PGACAAHH515 pKa = 6.96 PDD517 pKa = 3.26 VRR519 pKa = 11.84 DD520 pKa = 3.43 RR521 pKa = 11.84 VEE523 pKa = 3.88 AALPLTDD530 pKa = 4.05 YY531 pKa = 11.13 LWTRR535 pKa = 11.84 GQSPFAAPGEE545 pKa = 3.98 FLNLGVTAGIFVRR558 pKa = 11.84 HH559 pKa = 5.37 ATYY562 pKa = 10.57 TDD564 pKa = 3.94 DD565 pKa = 5.09 GDD567 pKa = 3.42 ITMSHH572 pKa = 6.77 LPSARR577 pKa = 11.84 EE578 pKa = 3.81 MVDD581 pKa = 2.83 MTVEE585 pKa = 4.03 IEE587 pKa = 4.12 VGRR590 pKa = 11.84 PASIANGASNAADD603 pKa = 3.39 AGTRR607 pKa = 11.84 RR608 pKa = 11.84 ARR610 pKa = 11.84 TRR612 pKa = 11.84 AARR615 pKa = 11.84 EE616 pKa = 3.43 LAAVKK621 pKa = 10.22 RR622 pKa = 11.84 RR623 pKa = 11.84 AALFGVADD631 pKa = 4.09 MFEE634 pKa = 4.34 MPVMTSAPRR643 pKa = 11.84 MDD645 pKa = 4.65 RR646 pKa = 11.84 PAPPAPPQAPGGPGAAPAPTRR667 pKa = 11.84 PPQQGVGAGPGAAAPTGDD685 pKa = 4.01 PLPPVGHH692 pKa = 7.04 HH693 pKa = 5.87 DD694 pKa = 3.96 HH695 pKa = 6.51 YY696 pKa = 10.77 RR697 pKa = 11.84 APPGPRR703 pKa = 11.84 PGGPLGVRR711 pKa = 11.84 GGGVGAGGQGGPGAPPPPPPPQNDD735 pKa = 3.56 DD736 pKa = 3.53 DD737 pKa = 5.15 DD738 pKa = 4.53 DD739 pKa = 4.19 RR740 pKa = 11.84 RR741 pKa = 11.84 AAEE744 pKa = 4.06 PLAPADD750 pKa = 3.88 APAEE754 pKa = 3.99 RR755 pKa = 11.84 AAPQQ759 pKa = 3.08
Molecular weight: 79.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.746
IPC2_protein 5.588
IPC_protein 5.652
Toseland 6.16
ProMoST 6.008
Dawson 5.931
Bjellqvist 5.931
Wikipedia 5.944
Rodwell 5.906
Grimsley 6.364
Solomon 5.931
Lehninger 5.919
Nozaki 6.198
DTASelect 6.389
Thurlkill 6.402
EMBOSS 6.364
Sillero 6.3
Patrickios 4.228
IPC_peptide 5.944
IPC2_peptide 6.326
IPC2.peptide.svr19 6.169
Protein with the highest isoelectric point:
>tr|A0A386HVJ6|A0A386HVJ6_9VIRU RNA-directed RNA polymerase OS=Fusarium asiaticum victorivirus 1 OX=2268743 PE=3 SV=1
MM1 pKa = 7.17 HH2 pKa = 7.5 RR3 pKa = 11.84 SAARR7 pKa = 11.84 AAEE10 pKa = 3.94 FALLGGNLLTLARR23 pKa = 11.84 SIPGLVHH30 pKa = 7.05 SYY32 pKa = 10.47 DD33 pKa = 3.21 GHH35 pKa = 6.94 DD36 pKa = 3.87 FTRR39 pKa = 11.84 QLIQLQSDD47 pKa = 3.81 TAGLARR53 pKa = 11.84 VNMLLPAAVSLLLADD68 pKa = 5.09 FPVQLSEE75 pKa = 5.58 GMALTNALARR85 pKa = 11.84 SAYY88 pKa = 9.79 SAPPYY93 pKa = 10.73 SLFPPEE99 pKa = 5.03 IIFSVPGVRR108 pKa = 11.84 HH109 pKa = 6.92 DD110 pKa = 3.85 KK111 pKa = 10.42 TSAPQRR117 pKa = 11.84 YY118 pKa = 8.64 ARR120 pKa = 11.84 GVFNILMHH128 pKa = 6.74 DD129 pKa = 3.79 KK130 pKa = 10.51 EE131 pKa = 4.43 VLARR135 pKa = 11.84 AFPRR139 pKa = 11.84 KK140 pKa = 9.3 AHH142 pKa = 6.21 AAANEE147 pKa = 4.02 KK148 pKa = 10.18 PLLHH152 pKa = 6.85 LGGLLAGLRR161 pKa = 11.84 KK162 pKa = 8.1 TRR164 pKa = 11.84 GVGAAAMMVVRR175 pKa = 11.84 AAGRR179 pKa = 11.84 LTEE182 pKa = 4.38 DD183 pKa = 3.31 QVISLILYY191 pKa = 7.32 QTALTPLFGQQSFDD205 pKa = 4.02 LAWWALTEE213 pKa = 3.93 PANAKK218 pKa = 9.98 GLSNGLKK225 pKa = 10.55 ALGLNATAHH234 pKa = 5.96 GAALVEE240 pKa = 4.26 ANAMQGRR247 pKa = 11.84 ATAPVDD253 pKa = 3.25 MDD255 pKa = 3.92 AEE257 pKa = 4.36 VAFRR261 pKa = 11.84 VEE263 pKa = 4.23 PARR266 pKa = 11.84 VAAKK270 pKa = 10.38 VLAPDD275 pKa = 3.59 EE276 pKa = 4.35 GDD278 pKa = 2.97 IRR280 pKa = 11.84 SHH282 pKa = 6.03 IRR284 pKa = 11.84 HH285 pKa = 6.65 ILTQEE290 pKa = 3.68 LRR292 pKa = 11.84 GDD294 pKa = 3.93 LEE296 pKa = 4.81 LPPLSDD302 pKa = 3.7 FWSSRR307 pKa = 11.84 WLWCVNGSNTSTSDD321 pKa = 2.77 RR322 pKa = 11.84 AMGLNPDD329 pKa = 4.61 EE330 pKa = 4.59 YY331 pKa = 11.15 ASTHH335 pKa = 4.0 TRR337 pKa = 11.84 TYY339 pKa = 10.62 RR340 pKa = 11.84 RR341 pKa = 11.84 MVAEE345 pKa = 4.14 TLEE348 pKa = 4.29 KK349 pKa = 10.77 EE350 pKa = 5.0 PISSWDD356 pKa = 3.13 GTTSVSASVKK366 pKa = 10.81 LEE368 pKa = 4.04 TAKK371 pKa = 10.7 SRR373 pKa = 11.84 AIFACDD379 pKa = 3.03 TRR381 pKa = 11.84 SYY383 pKa = 11.08 FAFSWILNDD392 pKa = 3.42 VQKK395 pKa = 10.63 RR396 pKa = 11.84 WRR398 pKa = 11.84 NDD400 pKa = 3.02 RR401 pKa = 11.84 VLLDD405 pKa = 3.89 PGKK408 pKa = 11.03 GGMAGIDD415 pKa = 3.23 RR416 pKa = 11.84 RR417 pKa = 11.84 IRR419 pKa = 11.84 AAGRR423 pKa = 11.84 RR424 pKa = 11.84 GGVNVMLDD432 pKa = 3.4 YY433 pKa = 11.58 DD434 pKa = 4.74 DD435 pKa = 5.9 FNSHH439 pKa = 6.21 HH440 pKa = 6.28 STRR443 pKa = 11.84 TMQVLFEE450 pKa = 4.43 EE451 pKa = 5.27 LCHH454 pKa = 7.33 LYY456 pKa = 10.08 HH457 pKa = 7.24 APQWYY462 pKa = 9.59 EE463 pKa = 3.8 DD464 pKa = 3.78 ALVASFDD471 pKa = 3.39 RR472 pKa = 11.84 SYY474 pKa = 9.96 ITWGGRR480 pKa = 11.84 KK481 pKa = 8.87 HH482 pKa = 6.29 RR483 pKa = 11.84 VLGTLMSGHH492 pKa = 7.18 RR493 pKa = 11.84 GTTFINSVLNAAYY506 pKa = 9.38 IRR508 pKa = 11.84 AAVGGPKK515 pKa = 9.7 FDD517 pKa = 4.2 GLISLHH523 pKa = 6.45 TGDD526 pKa = 4.95 DD527 pKa = 3.83 VYY529 pKa = 11.29 IRR531 pKa = 11.84 APTLSDD537 pKa = 3.29 CAEE540 pKa = 4.02 ILDD543 pKa = 3.67 RR544 pKa = 11.84 TRR546 pKa = 11.84 DD547 pKa = 3.46 YY548 pKa = 11.27 GCRR551 pKa = 11.84 MNPTKK556 pKa = 10.53 QSIGFRR562 pKa = 11.84 RR563 pKa = 11.84 AEE565 pKa = 4.17 FLRR568 pKa = 11.84 MGIGDD573 pKa = 3.96 RR574 pKa = 11.84 AAYY577 pKa = 9.69 GYY579 pKa = 10.11 LARR582 pKa = 11.84 SVAALASGNWFNPNPLTPWEE602 pKa = 4.66 GFNSLITSARR612 pKa = 11.84 SIINRR617 pKa = 11.84 AGGGLRR623 pKa = 11.84 LGALLAPAVRR633 pKa = 11.84 HH634 pKa = 5.0 GHH636 pKa = 5.58 SVDD639 pKa = 3.05 LRR641 pKa = 11.84 RR642 pKa = 11.84 RR643 pKa = 11.84 AAIIDD648 pKa = 3.75 GEE650 pKa = 4.34 HH651 pKa = 6.95 AIEE654 pKa = 3.98 GHH656 pKa = 5.52 PVYY659 pKa = 10.86 NVDD662 pKa = 3.39 YY663 pKa = 10.77 VLRR666 pKa = 11.84 TYY668 pKa = 11.11 SCTLPKK674 pKa = 10.09 VDD676 pKa = 5.0 EE677 pKa = 4.47 VPIHH681 pKa = 6.04 PGWRR685 pKa = 11.84 TNATRR690 pKa = 11.84 DD691 pKa = 3.57 YY692 pKa = 10.89 LLDD695 pKa = 3.62 HH696 pKa = 6.74 VSPVEE701 pKa = 4.03 AEE703 pKa = 4.02 ALALAGADD711 pKa = 3.39 VTTSMVAASFGKK723 pKa = 10.61 GLDD726 pKa = 3.39 RR727 pKa = 11.84 SKK729 pKa = 11.02 LRR731 pKa = 11.84 SLPEE735 pKa = 3.45 FQLRR739 pKa = 11.84 RR740 pKa = 11.84 NRR742 pKa = 11.84 DD743 pKa = 3.07 RR744 pKa = 11.84 KK745 pKa = 10.34 ALGFADD751 pKa = 3.9 VGSLLTSIPRR761 pKa = 11.84 VGCLSGYY768 pKa = 9.4 PLINLVAGRR777 pKa = 11.84 LSTDD781 pKa = 3.3 EE782 pKa = 4.27 LRR784 pKa = 11.84 GLVLLAGGDD793 pKa = 3.67 ANAADD798 pKa = 3.79 IKK800 pKa = 11.24 LEE802 pKa = 4.07 AFGSEE807 pKa = 3.87 SRR809 pKa = 11.84 TKK811 pKa = 10.81 NIIGTLPFSDD821 pKa = 3.77 AVNLGKK827 pKa = 9.1 RR828 pKa = 11.84 TLSDD832 pKa = 3.5 NIYY835 pKa = 8.16 TLTPIFMM842 pKa = 5.56
Molecular weight: 91.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.717
IPC2_protein 8.609
IPC_protein 8.609
Toseland 9.004
ProMoST 9.004
Dawson 9.385
Bjellqvist 9.282
Wikipedia 9.604
Rodwell 9.472
Grimsley 9.458
Solomon 9.443
Lehninger 9.385
Nozaki 9.297
DTASelect 9.194
Thurlkill 9.238
EMBOSS 9.502
Sillero 9.414
Patrickios 4.787
IPC_peptide 9.428
IPC2_peptide 8.112
IPC2.peptide.svr19 7.731
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1601
759
842
800.5
85.52
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.866 ± 1.394
0.687 ± 0.115
5.746 ± 0.058
3.935 ± 0.013
3.498 ± 0.043
9.557 ± 0.989
2.873 ± 0.018
3.935 ± 0.365
1.749 ± 0.782
9.744 ± 1.602
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.936 ± 0.16
3.31 ± 0.39
6.496 ± 1.483
1.936 ± 0.029
7.933 ± 0.492
6.184 ± 0.655
6.059 ± 0.285
6.621 ± 0.637
1.312 ± 0.185
2.623 ± 0.086
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here