Salinivibrio phage CW02
Average proteome isoelectric point is 5.92
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 70 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|H9D1I7|H9D1I7_9CAUD Uncharacterized protein OS=Salinivibrio phage CW02 OX=1161935 PE=4 SV=1
MM1 pKa = 7.79 LDD3 pKa = 4.82 KK4 pKa = 10.94 IMCYY8 pKa = 10.31 LLALFPVFAAITLYY22 pKa = 10.13 MGWPGEE28 pKa = 4.19 LILWYY33 pKa = 9.63 CVPIGLVVVVAEE45 pKa = 4.54 VYY47 pKa = 10.89 DD48 pKa = 5.37 LITVDD53 pKa = 3.78 PDD55 pKa = 3.5 DD56 pKa = 4.52 YY57 pKa = 11.5 EE58 pKa = 4.01
Molecular weight: 6.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.082
IPC2_protein 3.49
IPC_protein 3.3
Toseland 3.147
ProMoST 3.516
Dawson 3.35
Bjellqvist 3.681
Wikipedia 3.376
Rodwell 3.185
Grimsley 3.096
Solomon 3.236
Lehninger 3.185
Nozaki 3.541
DTASelect 3.656
Thurlkill 3.274
EMBOSS 3.376
Sillero 3.452
Patrickios 0.006
IPC_peptide 3.223
IPC2_peptide 3.401
IPC2.peptide.svr19 3.719
Protein with the highest isoelectric point:
>tr|H9D1G6|H9D1G6_9CAUD Uncharacterized protein OS=Salinivibrio phage CW02 OX=1161935 PE=4 SV=1
MM1 pKa = 7.45 GIEE4 pKa = 4.27 VAVGAAIVGSITTGVMAYY22 pKa = 9.83 KK23 pKa = 10.31 SYY25 pKa = 11.26 KK26 pKa = 7.21 EE27 pKa = 3.85 QKK29 pKa = 9.7 KK30 pKa = 9.66 AANIQRR36 pKa = 11.84 EE37 pKa = 3.99 QAKK40 pKa = 8.79 QQEE43 pKa = 4.75 AVNEE47 pKa = 4.16 AQASQQRR54 pKa = 11.84 QSAARR59 pKa = 11.84 EE60 pKa = 3.82 RR61 pKa = 11.84 RR62 pKa = 11.84 LKK64 pKa = 10.19 RR65 pKa = 11.84 ARR67 pKa = 11.84 ILQRR71 pKa = 11.84 AQAMGITGSSAVGQGQNALASGQDD95 pKa = 3.43 TMGSQQGFATAVAAGRR111 pKa = 11.84 SEE113 pKa = 4.31 TNQALANTQSNINRR127 pKa = 11.84 FNTIGKK133 pKa = 9.55 LGGLATQMGMSYY145 pKa = 11.31 AKK147 pKa = 10.54 GKK149 pKa = 10.15 VSQGG153 pKa = 3.08
Molecular weight: 16.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.294
IPC2_protein 9.823
IPC_protein 10.496
Toseland 10.862
ProMoST 10.482
Dawson 10.935
Bjellqvist 10.599
Wikipedia 11.125
Rodwell 11.286
Grimsley 10.979
Solomon 11.038
Lehninger 11.023
Nozaki 10.818
DTASelect 10.599
Thurlkill 10.847
EMBOSS 11.257
Sillero 10.862
Patrickios 11.023
IPC_peptide 11.052
IPC2_peptide 9.253
IPC2.peptide.svr19 8.64
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
70
0
70
15166
58
1413
216.7
24.28
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.24 ± 0.495
1.068 ± 0.171
6.515 ± 0.272
7.701 ± 0.27
3.686 ± 0.203
7.543 ± 0.295
1.853 ± 0.152
4.846 ± 0.178
6.6 ± 0.328
8.209 ± 0.271
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.585 ± 0.155
4.787 ± 0.203
3.613 ± 0.182
3.877 ± 0.331
5.077 ± 0.271
6.805 ± 0.353
5.486 ± 0.218
7.339 ± 0.206
1.444 ± 0.118
3.725 ± 0.178
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here