Equus caballus papillomavirus 1 (strain Olson) (EcPV-1)
Average proteome isoelectric point is 6.42
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q8QQC2|Q8QQC2_ECPVO Protein E6 OS=Equus caballus papillomavirus 1 (strain Olson) OX=654918 GN=E6 PE=3 SV=1
MM1 pKa = 7.66 IGNGSPSLRR10 pKa = 11.84 EE11 pKa = 3.71 IVLSEE16 pKa = 4.4 LPQSLADD23 pKa = 3.64 PAEE26 pKa = 4.43 AEE28 pKa = 4.24 SEE30 pKa = 4.2 EE31 pKa = 4.48 EE32 pKa = 4.04 EE33 pKa = 5.03 VEE35 pKa = 4.29 VEE37 pKa = 3.95 LDD39 pKa = 3.34 AVRR42 pKa = 11.84 PQAPYY47 pKa = 10.4 AVCTVCCRR55 pKa = 11.84 CGEE58 pKa = 4.17 KK59 pKa = 10.62 VGLCVLATDD68 pKa = 4.21 EE69 pKa = 5.31 GIHH72 pKa = 6.0 GLEE75 pKa = 3.84 EE76 pKa = 4.25 LLFEE80 pKa = 4.98 ALQLFCAQCAPPIGRR95 pKa = 11.84 HH96 pKa = 4.7 GRR98 pKa = 3.45
Molecular weight: 10.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.078
IPC2_protein 4.368
IPC_protein 4.228
Toseland 4.088
ProMoST 4.304
Dawson 4.139
Bjellqvist 4.291
Wikipedia 3.973
Rodwell 4.062
Grimsley 4.012
Solomon 4.139
Lehninger 4.088
Nozaki 4.266
DTASelect 4.304
Thurlkill 4.088
EMBOSS 3.999
Sillero 4.329
Patrickios 0.947
IPC_peptide 4.151
IPC2_peptide 4.317
IPC2.peptide.svr19 4.238
Protein with the highest isoelectric point:
>tr|Q8QQB9|Q8QQB9_ECPVO Regulatory protein E2 OS=Equus caballus papillomavirus 1 (strain Olson) OX=654918 GN=E2 PE=3 SV=1
PP1 pKa = 7.59 ALRR4 pKa = 11.84 LSLHH8 pKa = 5.85 LTSTSLTEE16 pKa = 3.97 QQEE19 pKa = 3.78 EE20 pKa = 4.14 DD21 pKa = 4.08 LINRR25 pKa = 11.84 LGLEE29 pKa = 3.94 PWQCRR34 pKa = 11.84 LRR36 pKa = 11.84 SPQKK40 pKa = 10.5 RR41 pKa = 11.84 GTALQLTQSAALGCQGGSAAQPTGAAGSYY70 pKa = 8.72 NARR73 pKa = 11.84 LPPPGCRR80 pKa = 11.84 RR81 pKa = 11.84 RR82 pKa = 11.84 YY83 pKa = 9.31 RR84 pKa = 11.84 KK85 pKa = 9.22 YY86 pKa = 10.03 LKK88 pKa = 10.05 RR89 pKa = 11.84 RR90 pKa = 11.84 RR91 pKa = 11.84 DD92 pKa = 3.12 QYY94 pKa = 11.34 RR95 pKa = 11.84 RR96 pKa = 11.84 LGEE99 pKa = 4.01 HH100 pKa = 5.75 LQEE103 pKa = 6.13 LIQQLDD109 pKa = 3.46 GGTTPRR115 pKa = 11.84 PYY117 pKa = 9.97 HH118 pKa = 5.37 WRR120 pKa = 11.84 LRR122 pKa = 11.84 SPQQ125 pKa = 3.18
Molecular weight: 14.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.307
IPC2_protein 9.867
IPC_protein 10.979
Toseland 10.833
ProMoST 10.979
Dawson 10.935
Bjellqvist 10.818
Wikipedia 11.286
Rodwell 10.833
Grimsley 11.008
Solomon 11.213
Lehninger 11.14
Nozaki 10.847
DTASelect 10.804
Thurlkill 10.862
EMBOSS 11.286
Sillero 10.906
Patrickios 10.54
IPC_peptide 11.213
IPC2_peptide 10.204
IPC2.peptide.svr19 8.559
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2372
98
620
338.9
37.82
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.45 ± 0.525
2.234 ± 0.672
5.523 ± 0.568
6.282 ± 0.607
3.626 ± 0.591
7.884 ± 1.127
2.024 ± 0.122
3.583 ± 0.323
3.836 ± 0.732
9.106 ± 0.613
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.855 ± 0.247
3.668 ± 0.69
7.125 ± 0.73
4.511 ± 0.715
7.083 ± 0.63
7.42 ± 0.763
5.776 ± 0.545
6.914 ± 0.664
1.939 ± 0.344
3.162 ± 0.256
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here