Carrot Ch virus 1

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Betaflexiviridae; Trivirinae; Chordovirus

Average proteome isoelectric point is 6.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A0A0P710|A0A0A0P710_9VIRU ORF1 OS=Carrot Ch virus 1 OX=1425363 PE=4 SV=1
MM1 pKa = 7.52SLVDD5 pKa = 3.78VNKK8 pKa = 9.99FCSLVSGGKK17 pKa = 9.85SSTEE21 pKa = 4.32AISSSNIYY29 pKa = 10.58GDD31 pKa = 4.31SSWLKK36 pKa = 10.29LKK38 pKa = 10.87NITAIKK44 pKa = 10.2KK45 pKa = 9.69FEE47 pKa = 4.36TNIEE51 pKa = 4.12LEE53 pKa = 4.43LEE55 pKa = 4.77DD56 pKa = 5.11GDD58 pKa = 4.4SKK60 pKa = 11.46LVIPNLPLISDD71 pKa = 3.81SEE73 pKa = 3.9IRR75 pKa = 11.84AVKK78 pKa = 10.61KK79 pKa = 10.63NMPSVNFVHH88 pKa = 6.96LGGIVVSIQALFAANKK104 pKa = 8.49GVKK107 pKa = 8.37GTAVLVDD114 pKa = 3.68RR115 pKa = 11.84RR116 pKa = 11.84WRR118 pKa = 11.84NLDD121 pKa = 3.09QAIIGSFAFNLDD133 pKa = 2.89KK134 pKa = 11.02RR135 pKa = 11.84RR136 pKa = 11.84ADD138 pKa = 3.54FMMRR142 pKa = 11.84PNFDD146 pKa = 3.08VSLKK150 pKa = 10.69DD151 pKa = 3.56SQLSDD156 pKa = 3.51SLSIMLNFEE165 pKa = 4.27NLDD168 pKa = 3.67MFAGSVPINISIGLIARR185 pKa = 11.84FYY187 pKa = 9.58NTIDD191 pKa = 3.29PGVRR195 pKa = 11.84IISDD199 pKa = 3.45DD200 pKa = 4.1LNFQGLIGAEE210 pKa = 4.46GISNQDD216 pKa = 3.21MLSNLGDD223 pKa = 3.7MKK225 pKa = 11.29DD226 pKa = 3.58LFSDD230 pKa = 3.68SVIFKK235 pKa = 8.41PTIVSNSKK243 pKa = 10.0FDD245 pKa = 3.51RR246 pKa = 11.84GMFKK250 pKa = 10.68SKK252 pKa = 10.72GLIKK256 pKa = 10.1QIRR259 pKa = 11.84GARR262 pKa = 11.84SGKK265 pKa = 10.03EE266 pKa = 3.57IISTGEE272 pKa = 3.69VKK274 pKa = 10.43RR275 pKa = 11.84PKK277 pKa = 10.22SSSFRR282 pKa = 11.84FDD284 pKa = 3.19QLKK287 pKa = 10.2VDD289 pKa = 3.54PVLYY293 pKa = 10.62DD294 pKa = 3.19KK295 pKa = 11.35LKK297 pKa = 10.43RR298 pKa = 11.84DD299 pKa = 4.69QVNLWLNKK307 pKa = 10.13NNEE310 pKa = 4.06LPIKK314 pKa = 10.27NVEE317 pKa = 4.18DD318 pKa = 3.84EE319 pKa = 5.06EE320 pKa = 4.67IVHH323 pKa = 7.36DD324 pKa = 4.77DD325 pKa = 3.62NSSGRR330 pKa = 11.84VDD332 pKa = 3.17IFEE335 pKa = 4.94HH336 pKa = 6.61GNN338 pKa = 3.16

Molecular weight:
37.59 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A0A0P9W1|A0A0A0P9W1_9VIRU Coat protein OS=Carrot Ch virus 1 OX=1425363 PE=4 SV=1
MM1 pKa = 7.28SCRR4 pKa = 11.84SRR6 pKa = 11.84MLKK9 pKa = 8.28TRR11 pKa = 11.84RR12 pKa = 11.84SFMMTILLEE21 pKa = 4.07EE22 pKa = 5.22LISLNTGTEE31 pKa = 3.56IGKK34 pKa = 10.53SIDD37 pKa = 3.56SLDD40 pKa = 4.52LIGNSLLNNYY50 pKa = 8.96RR51 pKa = 11.84DD52 pKa = 3.36HH53 pKa = 7.13FLINMAEE60 pKa = 4.46LGTSPDD66 pKa = 3.18TLYY69 pKa = 11.22EE70 pKa = 4.02MEE72 pKa = 4.78PFEE75 pKa = 4.62LPKK78 pKa = 10.08VTLVSNGEE86 pKa = 4.01KK87 pKa = 9.78KK88 pKa = 10.37DD89 pKa = 3.8YY90 pKa = 9.28KK91 pKa = 10.81LKK93 pKa = 10.36MSIGSAVNSIMNQIEE108 pKa = 4.24GDD110 pKa = 3.82HH111 pKa = 7.02RR112 pKa = 11.84LLTCNTTLRR121 pKa = 11.84NTLGAFGTEE130 pKa = 4.35TKK132 pKa = 10.44RR133 pKa = 11.84QLKK136 pKa = 8.8NRR138 pKa = 11.84KK139 pKa = 8.63IDD141 pKa = 4.18GILARR146 pKa = 11.84KK147 pKa = 8.67YY148 pKa = 10.53PKK150 pKa = 10.0LCKK153 pKa = 9.55QAMHH157 pKa = 6.58MGFDD161 pKa = 4.42FNQDD165 pKa = 2.54IPTNEE170 pKa = 3.79LSKK173 pKa = 11.28SEE175 pKa = 4.18MKK177 pKa = 10.47VKK179 pKa = 10.39QYY181 pKa = 11.14LYY183 pKa = 9.95QQLKK187 pKa = 9.49RR188 pKa = 11.84KK189 pKa = 6.93EE190 pKa = 4.08ARR192 pKa = 11.84RR193 pKa = 11.84GQYY196 pKa = 10.1EE197 pKa = 3.91EE198 pKa = 5.53DD199 pKa = 3.14ISVVDD204 pKa = 5.41FEE206 pKa = 4.7

Molecular weight:
23.75 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

2401

206

1857

800.3

91.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.373 ± 0.453

1.208 ± 0.274

7.08 ± 0.587

6.497 ± 0.647

6.039 ± 0.82

5.623 ± 0.391

1.749 ± 0.306

7.455 ± 0.547

8.705 ± 0.222

10.162 ± 0.557

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.207 ± 0.599

5.748 ± 0.675

2.874 ± 0.126

2.457 ± 0.41

4.831 ± 0.425

9.08 ± 0.731

3.915 ± 0.877

5.248 ± 0.761

0.916 ± 0.271

2.832 ± 0.585

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski