Carrot Ch virus 1
Average proteome isoelectric point is 6.85
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0A0P710|A0A0A0P710_9VIRU ORF1 OS=Carrot Ch virus 1 OX=1425363 PE=4 SV=1
MM1 pKa = 7.52 SLVDD5 pKa = 3.78 VNKK8 pKa = 9.99 FCSLVSGGKK17 pKa = 9.85 SSTEE21 pKa = 4.32 AISSSNIYY29 pKa = 10.58 GDD31 pKa = 4.31 SSWLKK36 pKa = 10.29 LKK38 pKa = 10.87 NITAIKK44 pKa = 10.2 KK45 pKa = 9.69 FEE47 pKa = 4.36 TNIEE51 pKa = 4.12 LEE53 pKa = 4.43 LEE55 pKa = 4.77 DD56 pKa = 5.11 GDD58 pKa = 4.4 SKK60 pKa = 11.46 LVIPNLPLISDD71 pKa = 3.81 SEE73 pKa = 3.9 IRR75 pKa = 11.84 AVKK78 pKa = 10.61 KK79 pKa = 10.63 NMPSVNFVHH88 pKa = 6.96 LGGIVVSIQALFAANKK104 pKa = 8.49 GVKK107 pKa = 8.37 GTAVLVDD114 pKa = 3.68 RR115 pKa = 11.84 RR116 pKa = 11.84 WRR118 pKa = 11.84 NLDD121 pKa = 3.09 QAIIGSFAFNLDD133 pKa = 2.89 KK134 pKa = 11.02 RR135 pKa = 11.84 RR136 pKa = 11.84 ADD138 pKa = 3.54 FMMRR142 pKa = 11.84 PNFDD146 pKa = 3.08 VSLKK150 pKa = 10.69 DD151 pKa = 3.56 SQLSDD156 pKa = 3.51 SLSIMLNFEE165 pKa = 4.27 NLDD168 pKa = 3.67 MFAGSVPINISIGLIARR185 pKa = 11.84 FYY187 pKa = 9.58 NTIDD191 pKa = 3.29 PGVRR195 pKa = 11.84 IISDD199 pKa = 3.45 DD200 pKa = 4.1 LNFQGLIGAEE210 pKa = 4.46 GISNQDD216 pKa = 3.21 MLSNLGDD223 pKa = 3.7 MKK225 pKa = 11.29 DD226 pKa = 3.58 LFSDD230 pKa = 3.68 SVIFKK235 pKa = 8.41 PTIVSNSKK243 pKa = 10.0 FDD245 pKa = 3.51 RR246 pKa = 11.84 GMFKK250 pKa = 10.68 SKK252 pKa = 10.72 GLIKK256 pKa = 10.1 QIRR259 pKa = 11.84 GARR262 pKa = 11.84 SGKK265 pKa = 10.03 EE266 pKa = 3.57 IISTGEE272 pKa = 3.69 VKK274 pKa = 10.43 RR275 pKa = 11.84 PKK277 pKa = 10.22 SSSFRR282 pKa = 11.84 FDD284 pKa = 3.19 QLKK287 pKa = 10.2 VDD289 pKa = 3.54 PVLYY293 pKa = 10.62 DD294 pKa = 3.19 KK295 pKa = 11.35 LKK297 pKa = 10.43 RR298 pKa = 11.84 DD299 pKa = 4.69 QVNLWLNKK307 pKa = 10.13 NNEE310 pKa = 4.06 LPIKK314 pKa = 10.27 NVEE317 pKa = 4.18 DD318 pKa = 3.84 EE319 pKa = 5.06 EE320 pKa = 4.67 IVHH323 pKa = 7.36 DD324 pKa = 4.77 DD325 pKa = 3.62 NSSGRR330 pKa = 11.84 VDD332 pKa = 3.17 IFEE335 pKa = 4.94 HH336 pKa = 6.61 GNN338 pKa = 3.16
Molecular weight: 37.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.195
IPC2_protein 6.059
IPC_protein 6.173
Toseland 6.517
ProMoST 6.453
Dawson 6.415
Bjellqvist 6.364
Wikipedia 6.427
Rodwell 6.389
Grimsley 6.649
Solomon 6.427
Lehninger 6.415
Nozaki 6.62
DTASelect 6.839
Thurlkill 6.825
EMBOSS 6.81
Sillero 6.751
Patrickios 5.105
IPC_peptide 6.44
IPC2_peptide 6.504
IPC2.peptide.svr19 6.763
Protein with the highest isoelectric point:
>tr|A0A0A0P9W1|A0A0A0P9W1_9VIRU Coat protein OS=Carrot Ch virus 1 OX=1425363 PE=4 SV=1
MM1 pKa = 7.28 SCRR4 pKa = 11.84 SRR6 pKa = 11.84 MLKK9 pKa = 8.28 TRR11 pKa = 11.84 RR12 pKa = 11.84 SFMMTILLEE21 pKa = 4.07 EE22 pKa = 5.22 LISLNTGTEE31 pKa = 3.56 IGKK34 pKa = 10.53 SIDD37 pKa = 3.56 SLDD40 pKa = 4.52 LIGNSLLNNYY50 pKa = 8.96 RR51 pKa = 11.84 DD52 pKa = 3.36 HH53 pKa = 7.13 FLINMAEE60 pKa = 4.46 LGTSPDD66 pKa = 3.18 TLYY69 pKa = 11.22 EE70 pKa = 4.02 MEE72 pKa = 4.78 PFEE75 pKa = 4.62 LPKK78 pKa = 10.08 VTLVSNGEE86 pKa = 4.01 KK87 pKa = 9.78 KK88 pKa = 10.37 DD89 pKa = 3.8 YY90 pKa = 9.28 KK91 pKa = 10.81 LKK93 pKa = 10.36 MSIGSAVNSIMNQIEE108 pKa = 4.24 GDD110 pKa = 3.82 HH111 pKa = 7.02 RR112 pKa = 11.84 LLTCNTTLRR121 pKa = 11.84 NTLGAFGTEE130 pKa = 4.35 TKK132 pKa = 10.44 RR133 pKa = 11.84 QLKK136 pKa = 8.8 NRR138 pKa = 11.84 KK139 pKa = 8.63 IDD141 pKa = 4.18 GILARR146 pKa = 11.84 KK147 pKa = 8.67 YY148 pKa = 10.53 PKK150 pKa = 10.0 LCKK153 pKa = 9.55 QAMHH157 pKa = 6.58 MGFDD161 pKa = 4.42 FNQDD165 pKa = 2.54 IPTNEE170 pKa = 3.79 LSKK173 pKa = 11.28 SEE175 pKa = 4.18 MKK177 pKa = 10.47 VKK179 pKa = 10.39 QYY181 pKa = 11.14 LYY183 pKa = 9.95 QQLKK187 pKa = 9.49 RR188 pKa = 11.84 KK189 pKa = 6.93 EE190 pKa = 4.08 ARR192 pKa = 11.84 RR193 pKa = 11.84 GQYY196 pKa = 10.1 EE197 pKa = 3.91 EE198 pKa = 5.53 DD199 pKa = 3.14 ISVVDD204 pKa = 5.41 FEE206 pKa = 4.7
Molecular weight: 23.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.013
IPC2_protein 8.068
IPC_protein 8.053
Toseland 8.507
ProMoST 8.492
Dawson 8.873
Bjellqvist 8.873
Wikipedia 8.916
Rodwell 8.99
Grimsley 8.448
Solomon 9.063
Lehninger 9.048
Nozaki 9.033
DTASelect 8.726
Thurlkill 8.843
EMBOSS 9.063
Sillero 9.063
Patrickios 4.762
IPC_peptide 9.048
IPC2_peptide 7.571
IPC2.peptide.svr19 7.616
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
2401
206
1857
800.3
91.36
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.373 ± 0.453
1.208 ± 0.274
7.08 ± 0.587
6.497 ± 0.647
6.039 ± 0.82
5.623 ± 0.391
1.749 ± 0.306
7.455 ± 0.547
8.705 ± 0.222
10.162 ± 0.557
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.207 ± 0.599
5.748 ± 0.675
2.874 ± 0.126
2.457 ± 0.41
4.831 ± 0.425
9.08 ± 0.731
3.915 ± 0.877
5.248 ± 0.761
0.916 ± 0.271
2.832 ± 0.585
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here