Streptomyces lavendulae subsp. lavendulae
Average proteome isoelectric point is 6.53
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7724 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2K8PQU3|A0A2K8PQU3_STRLA Transglutaminase-activating metalloprotease OS=Streptomyces lavendulae subsp. lavendulae OX=58340 GN=SLAV_36570 PE=4 SV=1
MM1 pKa = 7.67 SDD3 pKa = 4.01 DD4 pKa = 3.8 VLSIIPSDD12 pKa = 4.09 PQWQPDD18 pKa = 3.57 RR19 pKa = 11.84 DD20 pKa = 3.91 VVDD23 pKa = 3.83 RR24 pKa = 11.84 VIALVEE30 pKa = 4.03 DD31 pKa = 4.57 LAPGVVDD38 pKa = 4.49 GVDD41 pKa = 3.45 VEE43 pKa = 5.97 IDD45 pKa = 3.76 ATWHH49 pKa = 6.16 DD50 pKa = 4.1 VVTAVDD56 pKa = 3.84 CGDD59 pKa = 3.28 NLEE62 pKa = 5.37 RR63 pKa = 11.84 IGCPLCQAAIDD74 pKa = 4.13 TEE76 pKa = 4.08 WWGDD80 pKa = 3.64 LLEE83 pKa = 4.24 AHH85 pKa = 7.06 CDD87 pKa = 3.71 DD88 pKa = 5.08 GFTTLAIEE96 pKa = 4.63 VPCCGGSTTLDD107 pKa = 3.29 VLDD110 pKa = 4.62 YY111 pKa = 11.02 DD112 pKa = 4.37 WPCGFARR119 pKa = 11.84 FEE121 pKa = 3.81 IAIWNPEE128 pKa = 3.99 RR129 pKa = 11.84 LWFGEE134 pKa = 4.37 GEE136 pKa = 4.19 LTALADD142 pKa = 4.3 LLGHH146 pKa = 5.93 PVKK149 pKa = 10.59 QIRR152 pKa = 11.84 AHH154 pKa = 4.47 VV155 pKa = 3.43
Molecular weight: 17.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.724
IPC2_protein 3.757
IPC_protein 3.77
Toseland 3.541
ProMoST 3.935
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.719
Rodwell 3.592
Grimsley 3.452
Solomon 3.757
Lehninger 3.719
Nozaki 3.884
DTASelect 4.139
Thurlkill 3.605
EMBOSS 3.732
Sillero 3.884
Patrickios 1.888
IPC_peptide 3.757
IPC2_peptide 3.859
IPC2.peptide.svr19 3.807
Protein with the highest isoelectric point:
>tr|A0A2K8PGU4|A0A2K8PGU4_STRLA Penicillin-insensitive transglycosylase OS=Streptomyces lavendulae subsp. lavendulae OX=58340 GN=pbpG PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 AKK15 pKa = 8.7 THH17 pKa = 5.15 GFRR20 pKa = 11.84 LRR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 AILANRR35 pKa = 11.84 RR36 pKa = 11.84 SKK38 pKa = 10.78 GRR40 pKa = 11.84 AALSAA45 pKa = 3.96
Molecular weight: 5.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.272
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.647
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.369
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.218
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7724
0
7724
2527622
29
11445
327.2
34.84
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.422 ± 0.048
0.802 ± 0.007
5.726 ± 0.022
5.637 ± 0.028
2.686 ± 0.015
9.811 ± 0.029
2.279 ± 0.016
2.927 ± 0.021
1.992 ± 0.024
10.472 ± 0.039
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.677 ± 0.011
1.644 ± 0.014
6.492 ± 0.03
2.587 ± 0.016
8.163 ± 0.034
4.856 ± 0.017
5.988 ± 0.023
8.313 ± 0.027
1.525 ± 0.012
2.001 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here