Amino acid dipepetide frequency for Paenibacillus phage Gryphonian

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.296AlaAla: 5.296 ± 1.052
0.745AlaCys: 0.745 ± 0.193
4.634AlaAsp: 4.634 ± 0.699
6.289AlaGlu: 6.289 ± 0.63
2.648AlaPhe: 2.648 ± 0.449
4.634AlaGly: 4.634 ± 0.647
0.828AlaHis: 0.828 ± 0.263
4.717AlaIle: 4.717 ± 0.912
4.717AlaLys: 4.717 ± 0.731
6.124AlaLeu: 6.124 ± 0.783
1.407AlaMet: 1.407 ± 0.312
3.227AlaAsn: 3.227 ± 0.512
1.49AlaPro: 1.49 ± 0.356
2.234AlaGln: 2.234 ± 0.393
1.655AlaArg: 1.655 ± 0.31
3.889AlaSer: 3.889 ± 0.737
4.138AlaThr: 4.138 ± 0.874
4.138AlaVal: 4.138 ± 0.469
1.324AlaTrp: 1.324 ± 0.354
2.317AlaTyr: 2.317 ± 0.37
0.0AlaXaa: 0.0 ± 0.0
Cys
0.579CysAla: 0.579 ± 0.225
0.166CysCys: 0.166 ± 0.099
0.414CysAsp: 0.414 ± 0.182
0.662CysGlu: 0.662 ± 0.251
0.248CysPhe: 0.248 ± 0.132
0.745CysGly: 0.745 ± 0.245
0.083CysHis: 0.083 ± 0.087
0.745CysIle: 0.745 ± 0.253
0.91CysLys: 0.91 ± 0.283
1.076CysLeu: 1.076 ± 0.298
0.248CysMet: 0.248 ± 0.15
0.248CysAsn: 0.248 ± 0.131
0.414CysPro: 0.414 ± 0.224
0.331CysGln: 0.331 ± 0.157
0.497CysArg: 0.497 ± 0.171
0.662CysSer: 0.662 ± 0.187
0.166CysThr: 0.166 ± 0.117
0.414CysVal: 0.414 ± 0.187
0.083CysTrp: 0.083 ± 0.08
0.331CysTyr: 0.331 ± 0.169
0.0CysXaa: 0.0 ± 0.0
Asp
3.31AspAla: 3.31 ± 0.604
0.166AspCys: 0.166 ± 0.111
4.138AspAsp: 4.138 ± 0.71
4.386AspGlu: 4.386 ± 0.808
3.227AspPhe: 3.227 ± 0.622
3.641AspGly: 3.641 ± 0.536
1.076AspHis: 1.076 ± 0.34
3.889AspIle: 3.889 ± 0.548
4.22AspLys: 4.22 ± 0.601
4.8AspLeu: 4.8 ± 0.602
1.655AspMet: 1.655 ± 0.362
1.986AspAsn: 1.986 ± 0.311
1.738AspPro: 1.738 ± 0.336
2.234AspGln: 2.234 ± 0.477
2.896AspArg: 2.896 ± 0.505
2.814AspSer: 2.814 ± 0.421
2.814AspThr: 2.814 ± 0.542
4.303AspVal: 4.303 ± 0.53
0.91AspTrp: 0.91 ± 0.302
1.986AspTyr: 1.986 ± 0.383
0.0AspXaa: 0.0 ± 0.0
Glu
4.882GluAla: 4.882 ± 0.694
0.414GluCys: 0.414 ± 0.168
5.048GluAsp: 5.048 ± 0.787
5.793GluGlu: 5.793 ± 0.809
2.234GluPhe: 2.234 ± 0.333
4.138GluGly: 4.138 ± 0.52
1.159GluHis: 1.159 ± 0.33
6.538GluIle: 6.538 ± 0.984
7.696GluLys: 7.696 ± 0.638
7.117GluLeu: 7.117 ± 0.85
3.889GluMet: 3.889 ± 0.544
2.979GluAsn: 2.979 ± 0.475
2.234GluPro: 2.234 ± 0.316
4.303GluGln: 4.303 ± 0.782
3.145GluArg: 3.145 ± 0.663
4.138GluSer: 4.138 ± 0.604
5.214GluThr: 5.214 ± 0.553
5.793GluVal: 5.793 ± 0.731
1.076GluTrp: 1.076 ± 0.3
3.31GluTyr: 3.31 ± 0.614
0.0GluXaa: 0.0 ± 0.0
Phe
2.152PheAla: 2.152 ± 0.423
0.745PheCys: 0.745 ± 0.221
2.234PheAsp: 2.234 ± 0.371
2.896PheGlu: 2.896 ± 0.546
1.572PhePhe: 1.572 ± 0.551
2.234PheGly: 2.234 ± 0.393
0.91PheHis: 0.91 ± 0.26
2.979PheIle: 2.979 ± 0.509
2.648PheLys: 2.648 ± 0.452
3.558PheLeu: 3.558 ± 0.459
1.49PheMet: 1.49 ± 0.383
2.069PheAsn: 2.069 ± 0.394
1.076PhePro: 1.076 ± 0.311
1.49PheGln: 1.49 ± 0.341
1.324PheArg: 1.324 ± 0.41
2.317PheSer: 2.317 ± 0.483
2.069PheThr: 2.069 ± 0.364
2.814PheVal: 2.814 ± 0.495
0.414PheTrp: 0.414 ± 0.196
1.159PheTyr: 1.159 ± 0.281
0.0PheXaa: 0.0 ± 0.0
Gly
3.31GlyAla: 3.31 ± 0.539
0.579GlyCys: 0.579 ± 0.178
2.4GlyAsp: 2.4 ± 0.498
5.131GlyGlu: 5.131 ± 0.635
2.4GlyPhe: 2.4 ± 0.478
3.062GlyGly: 3.062 ± 0.541
1.159GlyHis: 1.159 ± 0.338
5.793GlyIle: 5.793 ± 1.019
6.455GlyLys: 6.455 ± 0.627
5.545GlyLeu: 5.545 ± 0.839
1.986GlyMet: 1.986 ± 0.565
3.476GlyAsn: 3.476 ± 0.543
0.993GlyPro: 0.993 ± 0.322
2.814GlyGln: 2.814 ± 0.608
2.896GlyArg: 2.896 ± 0.49
4.303GlySer: 4.303 ± 0.793
2.648GlyThr: 2.648 ± 0.596
3.476GlyVal: 3.476 ± 0.459
1.241GlyTrp: 1.241 ± 0.269
2.4GlyTyr: 2.4 ± 0.507
0.0GlyXaa: 0.0 ± 0.0
His
1.324HisAla: 1.324 ± 0.314
0.414HisCys: 0.414 ± 0.168
0.662HisAsp: 0.662 ± 0.3
1.407HisGlu: 1.407 ± 0.314
0.745HisPhe: 0.745 ± 0.274
0.497HisGly: 0.497 ± 0.211
0.497HisHis: 0.497 ± 0.192
2.4HisIle: 2.4 ± 0.394
0.91HisLys: 0.91 ± 0.352
2.234HisLeu: 2.234 ± 0.453
0.414HisMet: 0.414 ± 0.212
0.91HisAsn: 0.91 ± 0.258
1.076HisPro: 1.076 ± 0.268
0.331HisGln: 0.331 ± 0.143
1.241HisArg: 1.241 ± 0.261
1.407HisSer: 1.407 ± 0.318
0.331HisThr: 0.331 ± 0.15
1.324HisVal: 1.324 ± 0.41
0.248HisTrp: 0.248 ± 0.121
0.662HisTyr: 0.662 ± 0.213
0.0HisXaa: 0.0 ± 0.0
Ile
4.138IleAla: 4.138 ± 0.626
0.745IleCys: 0.745 ± 0.261
4.882IleAsp: 4.882 ± 0.683
6.786IleGlu: 6.786 ± 0.767
2.979IlePhe: 2.979 ± 0.531
4.469IleGly: 4.469 ± 0.769
1.49IleHis: 1.49 ± 0.325
3.724IleIle: 3.724 ± 0.755
6.455IleLys: 6.455 ± 1.005
5.545IleLeu: 5.545 ± 0.678
1.407IleMet: 1.407 ± 0.275
2.648IleAsn: 2.648 ± 0.41
2.4IlePro: 2.4 ± 0.412
3.641IleGln: 3.641 ± 0.526
3.889IleArg: 3.889 ± 0.634
3.476IleSer: 3.476 ± 0.544
4.551IleThr: 4.551 ± 0.504
5.048IleVal: 5.048 ± 0.626
0.91IleTrp: 0.91 ± 0.304
2.565IleTyr: 2.565 ± 0.409
0.0IleXaa: 0.0 ± 0.0
Lys
6.455LysAla: 6.455 ± 0.761
0.662LysCys: 0.662 ± 0.226
3.724LysAsp: 3.724 ± 0.412
8.524LysGlu: 8.524 ± 0.934
1.986LysPhe: 1.986 ± 0.324
5.876LysGly: 5.876 ± 0.671
1.655LysHis: 1.655 ± 0.333
5.296LysIle: 5.296 ± 0.555
7.944LysLys: 7.944 ± 1.041
6.703LysLeu: 6.703 ± 0.678
3.476LysMet: 3.476 ± 0.485
3.972LysAsn: 3.972 ± 0.526
2.896LysPro: 2.896 ± 0.535
4.469LysGln: 4.469 ± 0.505
5.214LysArg: 5.214 ± 0.624
5.131LysSer: 5.131 ± 0.783
5.627LysThr: 5.627 ± 0.722
4.386LysVal: 4.386 ± 0.559
1.241LysTrp: 1.241 ± 0.252
2.814LysTyr: 2.814 ± 0.532
0.0LysXaa: 0.0 ± 0.0
Leu
6.951LeuAla: 6.951 ± 0.685
0.662LeuCys: 0.662 ± 0.233
4.965LeuAsp: 4.965 ± 0.529
7.117LeuGlu: 7.117 ± 0.758
3.145LeuPhe: 3.145 ± 0.651
5.214LeuGly: 5.214 ± 0.731
1.738LeuHis: 1.738 ± 0.378
5.131LeuIle: 5.131 ± 0.632
6.538LeuLys: 6.538 ± 0.653
6.207LeuLeu: 6.207 ± 0.737
2.152LeuMet: 2.152 ± 0.542
4.469LeuAsn: 4.469 ± 0.548
3.062LeuPro: 3.062 ± 0.52
3.558LeuGln: 3.558 ± 0.404
3.641LeuArg: 3.641 ± 0.524
5.793LeuSer: 5.793 ± 0.624
5.214LeuThr: 5.214 ± 0.67
4.469LeuVal: 4.469 ± 0.484
1.324LeuTrp: 1.324 ± 0.413
3.227LeuTyr: 3.227 ± 0.513
0.0LeuXaa: 0.0 ± 0.0
Met
1.821MetAla: 1.821 ± 0.353
0.166MetCys: 0.166 ± 0.095
1.655MetAsp: 1.655 ± 0.325
2.648MetGlu: 2.648 ± 0.374
1.159MetPhe: 1.159 ± 0.295
1.076MetGly: 1.076 ± 0.282
0.331MetHis: 0.331 ± 0.144
2.069MetIle: 2.069 ± 0.441
3.641MetLys: 3.641 ± 0.549
2.731MetLeu: 2.731 ± 0.435
0.497MetMet: 0.497 ± 0.2
2.317MetAsn: 2.317 ± 0.451
1.241MetPro: 1.241 ± 0.336
1.159MetGln: 1.159 ± 0.267
1.324MetArg: 1.324 ± 0.296
2.152MetSer: 2.152 ± 0.348
1.655MetThr: 1.655 ± 0.348
0.91MetVal: 0.91 ± 0.321
0.331MetTrp: 0.331 ± 0.166
0.745MetTyr: 0.745 ± 0.216
0.0MetXaa: 0.0 ± 0.0
Asn
3.062AsnAla: 3.062 ± 0.467
0.745AsnCys: 0.745 ± 0.22
1.986AsnAsp: 1.986 ± 0.402
3.31AsnGlu: 3.31 ± 0.683
1.076AsnPhe: 1.076 ± 0.288
3.889AsnGly: 3.889 ± 0.533
0.91AsnHis: 0.91 ± 0.25
2.896AsnIle: 2.896 ± 0.452
3.807AsnLys: 3.807 ± 0.518
4.138AsnLeu: 4.138 ± 0.549
1.324AsnMet: 1.324 ± 0.411
1.738AsnAsn: 1.738 ± 0.49
2.152AsnPro: 2.152 ± 0.397
1.407AsnGln: 1.407 ± 0.28
2.648AsnArg: 2.648 ± 0.598
2.483AsnSer: 2.483 ± 0.394
2.069AsnThr: 2.069 ± 0.401
2.814AsnVal: 2.814 ± 0.468
0.579AsnTrp: 0.579 ± 0.223
2.069AsnTyr: 2.069 ± 0.345
0.0AsnXaa: 0.0 ± 0.0
Pro
2.483ProAla: 2.483 ± 0.388
0.166ProCys: 0.166 ± 0.095
1.324ProAsp: 1.324 ± 0.229
2.979ProGlu: 2.979 ± 0.749
1.241ProPhe: 1.241 ± 0.294
2.648ProGly: 2.648 ± 0.487
0.91ProHis: 0.91 ± 0.26
2.565ProIle: 2.565 ± 0.403
3.558ProLys: 3.558 ± 0.615
2.483ProLeu: 2.483 ± 0.493
0.828ProMet: 0.828 ± 0.261
1.821ProAsn: 1.821 ± 0.38
0.745ProPro: 0.745 ± 0.238
0.828ProGln: 0.828 ± 0.239
0.91ProArg: 0.91 ± 0.266
2.4ProSer: 2.4 ± 0.592
1.655ProThr: 1.655 ± 0.302
2.069ProVal: 2.069 ± 0.474
0.91ProTrp: 0.91 ± 0.28
0.828ProTyr: 0.828 ± 0.271
0.0ProXaa: 0.0 ± 0.0
Gln
2.979GlnAla: 2.979 ± 0.514
0.248GlnCys: 0.248 ± 0.145
2.152GlnAsp: 2.152 ± 0.316
3.393GlnGlu: 3.393 ± 0.603
1.572GlnPhe: 1.572 ± 0.3
2.483GlnGly: 2.483 ± 0.474
0.91GlnHis: 0.91 ± 0.279
3.393GlnIle: 3.393 ± 0.654
3.31GlnLys: 3.31 ± 0.568
4.303GlnLeu: 4.303 ± 0.884
1.655GlnMet: 1.655 ± 0.425
1.903GlnAsn: 1.903 ± 0.391
1.572GlnPro: 1.572 ± 0.401
1.655GlnGln: 1.655 ± 0.35
2.234GlnArg: 2.234 ± 0.423
2.4GlnSer: 2.4 ± 0.379
2.317GlnThr: 2.317 ± 0.41
1.903GlnVal: 1.903 ± 0.41
0.579GlnTrp: 0.579 ± 0.214
0.91GlnTyr: 0.91 ± 0.278
0.0GlnXaa: 0.0 ± 0.0
Arg
2.565ArgAla: 2.565 ± 0.436
0.497ArgCys: 0.497 ± 0.182
2.731ArgAsp: 2.731 ± 0.497
3.641ArgGlu: 3.641 ± 0.655
1.821ArgPhe: 1.821 ± 0.375
2.648ArgGly: 2.648 ± 0.537
0.91ArgHis: 0.91 ± 0.285
3.393ArgIle: 3.393 ± 0.559
5.627ArgLys: 5.627 ± 0.738
3.889ArgLeu: 3.889 ± 0.523
1.076ArgMet: 1.076 ± 0.306
2.317ArgAsn: 2.317 ± 0.416
1.407ArgPro: 1.407 ± 0.363
2.483ArgGln: 2.483 ± 0.448
2.152ArgArg: 2.152 ± 0.395
1.903ArgSer: 1.903 ± 0.363
1.986ArgThr: 1.986 ± 0.344
2.234ArgVal: 2.234 ± 0.395
0.662ArgTrp: 0.662 ± 0.213
1.821ArgTyr: 1.821 ± 0.337
0.0ArgXaa: 0.0 ± 0.0
Ser
4.8SerAla: 4.8 ± 0.985
0.414SerCys: 0.414 ± 0.179
4.22SerAsp: 4.22 ± 0.523
4.8SerGlu: 4.8 ± 0.724
2.731SerPhe: 2.731 ± 0.505
4.469SerGly: 4.469 ± 0.6
1.076SerHis: 1.076 ± 0.275
5.462SerIle: 5.462 ± 0.747
4.386SerLys: 4.386 ± 0.632
4.551SerLeu: 4.551 ± 0.701
1.49SerMet: 1.49 ± 0.301
2.069SerAsn: 2.069 ± 0.463
1.986SerPro: 1.986 ± 0.339
2.4SerGln: 2.4 ± 0.499
2.979SerArg: 2.979 ± 0.501
3.476SerSer: 3.476 ± 0.604
2.896SerThr: 2.896 ± 0.491
4.303SerVal: 4.303 ± 0.68
0.497SerTrp: 0.497 ± 0.194
1.738SerTyr: 1.738 ± 0.26
0.0SerXaa: 0.0 ± 0.0
Thr
4.22ThrAla: 4.22 ± 0.787
0.331ThrCys: 0.331 ± 0.153
2.317ThrAsp: 2.317 ± 0.388
3.724ThrGlu: 3.724 ± 0.447
2.069ThrPhe: 2.069 ± 0.435
4.055ThrGly: 4.055 ± 0.626
1.407ThrHis: 1.407 ± 0.394
4.22ThrIle: 4.22 ± 0.672
4.634ThrLys: 4.634 ± 0.582
4.22ThrLeu: 4.22 ± 0.692
1.572ThrMet: 1.572 ± 0.386
1.655ThrAsn: 1.655 ± 0.349
3.062ThrPro: 3.062 ± 0.551
2.317ThrGln: 2.317 ± 0.419
2.069ThrArg: 2.069 ± 0.36
4.138ThrSer: 4.138 ± 0.5
1.986ThrThr: 1.986 ± 0.442
4.055ThrVal: 4.055 ± 0.678
0.331ThrTrp: 0.331 ± 0.146
2.152ThrTyr: 2.152 ± 0.346
0.0ThrXaa: 0.0 ± 0.0
Val
3.972ValAla: 3.972 ± 0.619
0.414ValCys: 0.414 ± 0.19
3.145ValAsp: 3.145 ± 0.546
4.22ValGlu: 4.22 ± 0.585
2.896ValPhe: 2.896 ± 0.477
3.145ValGly: 3.145 ± 0.606
0.828ValHis: 0.828 ± 0.332
3.558ValIle: 3.558 ± 0.365
5.71ValLys: 5.71 ± 0.613
4.8ValLeu: 4.8 ± 0.517
1.738ValMet: 1.738 ± 0.32
2.565ValAsn: 2.565 ± 0.454
1.903ValPro: 1.903 ± 0.422
2.317ValGln: 2.317 ± 0.423
2.814ValArg: 2.814 ± 0.491
4.717ValSer: 4.717 ± 0.56
4.965ValThr: 4.965 ± 0.638
3.641ValVal: 3.641 ± 0.514
1.821ValTrp: 1.821 ± 0.926
2.483ValTyr: 2.483 ± 0.533
0.0ValXaa: 0.0 ± 0.0
Trp
0.662TrpAla: 0.662 ± 0.22
0.166TrpCys: 0.166 ± 0.111
0.91TrpAsp: 0.91 ± 0.315
0.828TrpGlu: 0.828 ± 0.21
0.91TrpPhe: 0.91 ± 0.237
1.076TrpGly: 1.076 ± 0.294
0.497TrpHis: 0.497 ± 0.187
0.579TrpIle: 0.579 ± 0.184
1.324TrpLys: 1.324 ± 0.38
1.407TrpLeu: 1.407 ± 0.334
0.331TrpMet: 0.331 ± 0.154
1.655TrpAsn: 1.655 ± 1.131
0.414TrpPro: 0.414 ± 0.224
0.745TrpGln: 0.745 ± 0.245
0.414TrpArg: 0.414 ± 0.176
0.828TrpSer: 0.828 ± 0.237
0.579TrpThr: 0.579 ± 0.204
0.745TrpVal: 0.745 ± 0.267
0.248TrpTrp: 0.248 ± 0.125
0.662TrpTyr: 0.662 ± 0.235
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.49TyrAla: 1.49 ± 0.365
0.579TyrCys: 0.579 ± 0.221
2.731TyrAsp: 2.731 ± 0.476
2.565TyrGlu: 2.565 ± 0.453
1.49TyrPhe: 1.49 ± 0.272
2.069TyrGly: 2.069 ± 0.441
0.662TyrHis: 0.662 ± 0.225
2.4TyrIle: 2.4 ± 0.395
3.558TyrLys: 3.558 ± 0.392
3.145TyrLeu: 3.145 ± 0.513
0.91TyrMet: 0.91 ± 0.279
1.076TyrAsn: 1.076 ± 0.287
1.49TyrPro: 1.49 ± 0.358
1.159TyrGln: 1.159 ± 0.276
1.821TyrArg: 1.821 ± 0.292
2.317TyrSer: 2.317 ± 0.464
1.655TyrThr: 1.655 ± 0.358
2.814TyrVal: 2.814 ± 0.404
0.248TyrTrp: 0.248 ± 0.138
1.821TyrTyr: 1.821 ± 0.382
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 66 proteins (12085 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski