Streptococcus satellite phage Javan301

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 7.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 19 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A4D5ZJW1|A0A4D5ZJW1_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan301 OX=2558621 GN=JavanS301_0015 PE=4 SV=1
MM1 pKa = 7.27NRR3 pKa = 11.84LKK5 pKa = 10.54EE6 pKa = 3.95LRR8 pKa = 11.84QGKK11 pKa = 9.48KK12 pKa = 8.47LTQQEE17 pKa = 4.18LAQEE21 pKa = 4.12IGVSKK26 pKa = 9.36LTILNWEE33 pKa = 4.31KK34 pKa = 11.15GEE36 pKa = 4.03HH37 pKa = 5.69QIKK40 pKa = 10.14SDD42 pKa = 3.77KK43 pKa = 10.71AQQLADD49 pKa = 3.99FFEE52 pKa = 4.83VPVGYY57 pKa = 10.66LLGYY61 pKa = 10.5DD62 pKa = 4.2DD63 pKa = 5.93YY64 pKa = 11.61KK65 pKa = 11.01TIQNDD70 pKa = 3.13AFDD73 pKa = 4.08SYY75 pKa = 11.93RR76 pKa = 11.84NMVKK80 pKa = 10.57LLLTNPDD87 pKa = 3.34FKK89 pKa = 11.43NIISEE94 pKa = 4.03YY95 pKa = 11.04DD96 pKa = 2.99EE97 pKa = 4.35TNRR100 pKa = 11.84KK101 pKa = 8.27NGKK104 pKa = 9.07RR105 pKa = 11.84DD106 pKa = 2.98LSLFVKK112 pKa = 10.69AEE114 pKa = 4.01SLPVIEE120 pKa = 5.77QDD122 pKa = 3.23IKK124 pKa = 11.61DD125 pKa = 4.58LILEE129 pKa = 4.32EE130 pKa = 4.25WKK132 pKa = 8.9KK133 pKa = 7.9TQPEE137 pKa = 4.06DD138 pKa = 3.34NYY140 pKa = 11.17EE141 pKa = 4.35EE142 pKa = 4.48IDD144 pKa = 3.64GTLSDD149 pKa = 4.57NISRR153 pKa = 11.84IYY155 pKa = 9.82IALGQLPILFQDD167 pKa = 3.99FFGSFLTLSKK177 pKa = 10.51SDD179 pKa = 3.7KK180 pKa = 10.33KK181 pKa = 10.95IVMQLVNSLYY191 pKa = 10.69EE192 pKa = 3.91KK193 pKa = 10.84NKK195 pKa = 10.68DD196 pKa = 2.79IGIIKK201 pKa = 10.34DD202 pKa = 3.73YY203 pKa = 10.18TDD205 pKa = 3.19KK206 pKa = 11.22KK207 pKa = 10.93

Molecular weight:
24.11 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A4D5ZNN3|A0A4D5ZNN3_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan301 OX=2558621 GN=JavanS301_0011 PE=4 SV=1
MM1 pKa = 7.77LYY3 pKa = 8.58KK4 pKa = 10.28TLLATGLRR12 pKa = 11.84IRR14 pKa = 11.84EE15 pKa = 4.14CLALEE20 pKa = 4.16WSDD23 pKa = 4.72IDD25 pKa = 4.27LQNGTIDD32 pKa = 3.28INKK35 pKa = 8.44TLNILNQVNSPKK47 pKa = 9.85TKK49 pKa = 9.48SSYY52 pKa = 9.99RR53 pKa = 11.84VLDD56 pKa = 3.58IDD58 pKa = 3.85HH59 pKa = 6.69KK60 pKa = 8.91TVLMLRR66 pKa = 11.84LYY68 pKa = 10.46RR69 pKa = 11.84ARR71 pKa = 11.84QAEE74 pKa = 4.01NGRR77 pKa = 11.84NIGLTYY83 pKa = 10.56EE84 pKa = 4.48KK85 pKa = 10.64VFSDD89 pKa = 4.51SFDD92 pKa = 3.64NYY94 pKa = 10.22VNTRR98 pKa = 11.84KK99 pKa = 9.81VDD101 pKa = 3.68YY102 pKa = 10.13RR103 pKa = 11.84LHH105 pKa = 6.06KK106 pKa = 9.82HH107 pKa = 6.03LKK109 pKa = 9.47SANCTDD115 pKa = 3.87LGFHH119 pKa = 7.4AFRR122 pKa = 11.84HH123 pKa = 4.54THH125 pKa = 6.71ASILLNAGLPYY136 pKa = 10.43KK137 pKa = 10.2EE138 pKa = 3.68IQTRR142 pKa = 11.84LGHH145 pKa = 6.33AKK147 pKa = 10.05ISVTMDD153 pKa = 3.68TYY155 pKa = 11.66SHH157 pKa = 7.23LSKK160 pKa = 10.56EE161 pKa = 4.22NQKK164 pKa = 10.33RR165 pKa = 11.84AVSFFEE171 pKa = 3.95NALEE175 pKa = 4.56KK176 pKa = 10.41IKK178 pKa = 11.0KK179 pKa = 9.06FLKK182 pKa = 10.51KK183 pKa = 8.91STKK186 pKa = 10.09

Molecular weight:
21.53 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

19

0

19

2999

53

496

157.8

18.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.235 ± 0.355

0.5 ± 0.139

6.802 ± 0.593

7.836 ± 0.528

4.568 ± 0.306

5.135 ± 0.5

1.434 ± 0.235

7.069 ± 0.566

11.204 ± 0.533

9.603 ± 0.447

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.134 ± 0.253

5.502 ± 0.34

2.568 ± 0.543

3.935 ± 0.559

4.802 ± 0.374

6.369 ± 0.399

6.202 ± 0.553

4.935 ± 0.512

0.834 ± 0.096

4.335 ± 0.277

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski