Streptococcus satellite phage Javan301
Average proteome isoelectric point is 7.09
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 19 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZJW1|A0A4D5ZJW1_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan301 OX=2558621 GN=JavanS301_0015 PE=4 SV=1
MM1 pKa = 7.27 NRR3 pKa = 11.84 LKK5 pKa = 10.54 EE6 pKa = 3.95 LRR8 pKa = 11.84 QGKK11 pKa = 9.48 KK12 pKa = 8.47 LTQQEE17 pKa = 4.18 LAQEE21 pKa = 4.12 IGVSKK26 pKa = 9.36 LTILNWEE33 pKa = 4.31 KK34 pKa = 11.15 GEE36 pKa = 4.03 HH37 pKa = 5.69 QIKK40 pKa = 10.14 SDD42 pKa = 3.77 KK43 pKa = 10.71 AQQLADD49 pKa = 3.99 FFEE52 pKa = 4.83 VPVGYY57 pKa = 10.66 LLGYY61 pKa = 10.5 DD62 pKa = 4.2 DD63 pKa = 5.93 YY64 pKa = 11.61 KK65 pKa = 11.01 TIQNDD70 pKa = 3.13 AFDD73 pKa = 4.08 SYY75 pKa = 11.93 RR76 pKa = 11.84 NMVKK80 pKa = 10.57 LLLTNPDD87 pKa = 3.34 FKK89 pKa = 11.43 NIISEE94 pKa = 4.03 YY95 pKa = 11.04 DD96 pKa = 2.99 EE97 pKa = 4.35 TNRR100 pKa = 11.84 KK101 pKa = 8.27 NGKK104 pKa = 9.07 RR105 pKa = 11.84 DD106 pKa = 2.98 LSLFVKK112 pKa = 10.69 AEE114 pKa = 4.01 SLPVIEE120 pKa = 5.77 QDD122 pKa = 3.23 IKK124 pKa = 11.61 DD125 pKa = 4.58 LILEE129 pKa = 4.32 EE130 pKa = 4.25 WKK132 pKa = 8.9 KK133 pKa = 7.9 TQPEE137 pKa = 4.06 DD138 pKa = 3.34 NYY140 pKa = 11.17 EE141 pKa = 4.35 EE142 pKa = 4.48 IDD144 pKa = 3.64 GTLSDD149 pKa = 4.57 NISRR153 pKa = 11.84 IYY155 pKa = 9.82 IALGQLPILFQDD167 pKa = 3.99 FFGSFLTLSKK177 pKa = 10.51 SDD179 pKa = 3.7 KK180 pKa = 10.33 KK181 pKa = 10.95 IVMQLVNSLYY191 pKa = 10.69 EE192 pKa = 3.91 KK193 pKa = 10.84 NKK195 pKa = 10.68 DD196 pKa = 2.79 IGIIKK201 pKa = 10.34 DD202 pKa = 3.73 YY203 pKa = 10.18 TDD205 pKa = 3.19 KK206 pKa = 11.22 KK207 pKa = 10.93
Molecular weight: 24.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.101
IPC2_protein 5.156
IPC_protein 5.041
Toseland 4.902
ProMoST 5.156
Dawson 5.003
Bjellqvist 5.143
Wikipedia 4.876
Rodwell 4.889
Grimsley 4.813
Solomon 4.991
Lehninger 4.952
Nozaki 5.105
DTASelect 5.283
Thurlkill 4.902
EMBOSS 4.889
Sillero 5.169
Patrickios 4.393
IPC_peptide 5.003
IPC2_peptide 5.156
IPC2.peptide.svr19 5.243
Protein with the highest isoelectric point:
>tr|A0A4D5ZNN3|A0A4D5ZNN3_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan301 OX=2558621 GN=JavanS301_0011 PE=4 SV=1
MM1 pKa = 7.77 LYY3 pKa = 8.58 KK4 pKa = 10.28 TLLATGLRR12 pKa = 11.84 IRR14 pKa = 11.84 EE15 pKa = 4.14 CLALEE20 pKa = 4.16 WSDD23 pKa = 4.72 IDD25 pKa = 4.27 LQNGTIDD32 pKa = 3.28 INKK35 pKa = 8.44 TLNILNQVNSPKK47 pKa = 9.85 TKK49 pKa = 9.48 SSYY52 pKa = 9.99 RR53 pKa = 11.84 VLDD56 pKa = 3.58 IDD58 pKa = 3.85 HH59 pKa = 6.69 KK60 pKa = 8.91 TVLMLRR66 pKa = 11.84 LYY68 pKa = 10.46 RR69 pKa = 11.84 ARR71 pKa = 11.84 QAEE74 pKa = 4.01 NGRR77 pKa = 11.84 NIGLTYY83 pKa = 10.56 EE84 pKa = 4.48 KK85 pKa = 10.64 VFSDD89 pKa = 4.51 SFDD92 pKa = 3.64 NYY94 pKa = 10.22 VNTRR98 pKa = 11.84 KK99 pKa = 9.81 VDD101 pKa = 3.68 YY102 pKa = 10.13 RR103 pKa = 11.84 LHH105 pKa = 6.06 KK106 pKa = 9.82 HH107 pKa = 6.03 LKK109 pKa = 9.47 SANCTDD115 pKa = 3.87 LGFHH119 pKa = 7.4 AFRR122 pKa = 11.84 HH123 pKa = 4.54 THH125 pKa = 6.71 ASILLNAGLPYY136 pKa = 10.43 KK137 pKa = 10.2 EE138 pKa = 3.68 IQTRR142 pKa = 11.84 LGHH145 pKa = 6.33 AKK147 pKa = 10.05 ISVTMDD153 pKa = 3.68 TYY155 pKa = 11.66 SHH157 pKa = 7.23 LSKK160 pKa = 10.56 EE161 pKa = 4.22 NQKK164 pKa = 10.33 RR165 pKa = 11.84 AVSFFEE171 pKa = 3.95 NALEE175 pKa = 4.56 KK176 pKa = 10.41 IKK178 pKa = 11.0 KK179 pKa = 9.06 FLKK182 pKa = 10.51 KK183 pKa = 8.91 STKK186 pKa = 10.09
Molecular weight: 21.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.06
IPC2_protein 9.209
IPC_protein 9.136
Toseland 9.94
ProMoST 9.633
Dawson 10.131
Bjellqvist 9.78
Wikipedia 10.277
Rodwell 10.628
Grimsley 10.204
Solomon 10.16
Lehninger 10.131
Nozaki 9.94
DTASelect 9.78
Thurlkill 9.984
EMBOSS 10.335
Sillero 10.043
Patrickios 10.218
IPC_peptide 10.16
IPC2_peptide 8.331
IPC2.peptide.svr19 8.133
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
19
0
19
2999
53
496
157.8
18.29
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.235 ± 0.355
0.5 ± 0.139
6.802 ± 0.593
7.836 ± 0.528
4.568 ± 0.306
5.135 ± 0.5
1.434 ± 0.235
7.069 ± 0.566
11.204 ± 0.533
9.603 ± 0.447
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.134 ± 0.253
5.502 ± 0.34
2.568 ± 0.543
3.935 ± 0.559
4.802 ± 0.374
6.369 ± 0.399
6.202 ± 0.553
4.935 ± 0.512
0.834 ± 0.096
4.335 ± 0.277
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here