Corynebacterium canis
Average proteome isoelectric point is 6.13
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2534 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5C5UG16|A0A5C5UG16_9CORY Acyl-CoA thioesterase II OS=Corynebacterium canis OX=679663 GN=FRX94_07710 PE=3 SV=1
MM1 pKa = 7.58 NEE3 pKa = 3.78 TEE5 pKa = 4.64 ALQEE9 pKa = 4.03 PATDD13 pKa = 3.04 VSFAVTVCRR22 pKa = 11.84 VPSGAWYY29 pKa = 10.04 VVGMDD34 pKa = 4.0 TDD36 pKa = 4.11 FSDD39 pKa = 4.98 IEE41 pKa = 4.25 DD42 pKa = 4.05 TVRR45 pKa = 11.84 KK46 pKa = 9.02 AQAICEE52 pKa = 4.13 HH53 pKa = 6.05 GQVFTMSCVDD63 pKa = 2.88 EE64 pKa = 5.48 DD65 pKa = 4.76 YY66 pKa = 11.08 FVLARR71 pKa = 11.84 HH72 pKa = 5.6 VGDD75 pKa = 3.5 RR76 pKa = 11.84 VEE78 pKa = 5.3 LLLSDD83 pKa = 3.42 ACMAVNDD90 pKa = 4.94 PYY92 pKa = 11.24 AASVLEE98 pKa = 4.15 KK99 pKa = 10.24 TGGCIPEE106 pKa = 4.8 LGEE109 pKa = 5.15 CDD111 pKa = 3.91 FADD114 pKa = 4.34 PCPEE118 pKa = 4.17 GNPGLLADD126 pKa = 5.42 LGLSADD132 pKa = 3.86 TMRR135 pKa = 11.84 AICNDD140 pKa = 3.07 NDD142 pKa = 4.47 LLPSAQLLCVAAEE155 pKa = 4.21 LGFASEE161 pKa = 4.83 FAQATDD167 pKa = 4.09 LNLDD171 pKa = 3.42
Molecular weight: 18.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.785
IPC2_protein 3.694
IPC_protein 3.681
Toseland 3.465
ProMoST 3.808
Dawson 3.681
Bjellqvist 3.872
Wikipedia 3.617
Rodwell 3.516
Grimsley 3.376
Solomon 3.668
Lehninger 3.617
Nozaki 3.795
DTASelect 4.024
Thurlkill 3.528
EMBOSS 3.617
Sillero 3.808
Patrickios 0.401
IPC_peptide 3.656
IPC2_peptide 3.783
IPC2.peptide.svr19 3.763
Protein with the highest isoelectric point:
>tr|A0A5C5UDK9|A0A5C5UDK9_9CORY Rieske 2Fe-2S domain-containing protein OS=Corynebacterium canis OX=679663 GN=FRX94_09740 PE=4 SV=1
MM1 pKa = 7.69 AKK3 pKa = 10.06 GKK5 pKa = 8.69 RR6 pKa = 11.84 TFQPNNRR13 pKa = 11.84 RR14 pKa = 11.84 RR15 pKa = 11.84 ARR17 pKa = 11.84 VHH19 pKa = 5.99 GFRR22 pKa = 11.84 TRR24 pKa = 11.84 MRR26 pKa = 11.84 TRR28 pKa = 11.84 AGRR31 pKa = 11.84 AIVSARR37 pKa = 11.84 RR38 pKa = 11.84 RR39 pKa = 11.84 KK40 pKa = 9.57 GRR42 pKa = 11.84 ASLTVV47 pKa = 3.04
Molecular weight: 5.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.345
IPC_protein 12.954
Toseland 13.115
ProMoST 13.612
Dawson 13.115
Bjellqvist 13.115
Wikipedia 13.598
Rodwell 12.705
Grimsley 13.159
Solomon 13.612
Lehninger 13.51
Nozaki 13.115
DTASelect 13.115
Thurlkill 13.115
EMBOSS 13.612
Sillero 13.115
Patrickios 12.427
IPC_peptide 13.612
IPC2_peptide 12.603
IPC2.peptide.svr19 9.265
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2534
0
2534
838831
33
3008
331.0
35.92
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.828 ± 0.053
0.908 ± 0.014
5.591 ± 0.036
6.098 ± 0.049
3.416 ± 0.03
8.172 ± 0.044
2.275 ± 0.023
5.163 ± 0.037
3.006 ± 0.041
9.914 ± 0.06
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.171 ± 0.022
2.727 ± 0.028
5.207 ± 0.042
3.412 ± 0.029
6.468 ± 0.051
5.48 ± 0.031
6.045 ± 0.039
8.442 ± 0.046
1.418 ± 0.02
2.259 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here