Hymenobacter sp. KIGAM108

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Hymenobacteraceae; Hymenobacter; unclassified Hymenobacter

Average proteome isoelectric point is 6.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4114 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5D6VG27|A0A5D6VG27_9BACT Uncharacterized protein OS=Hymenobacter sp. KIGAM108 OX=2606448 GN=FY528_01150 PE=4 SV=1
MM1 pKa = 8.43DD2 pKa = 3.81YY3 pKa = 11.36TNDD6 pKa = 3.5PEE8 pKa = 5.64LNGKK12 pKa = 8.58YY13 pKa = 10.13LGTITKK19 pKa = 10.4DD20 pKa = 3.03FATVSDD26 pKa = 4.14TLSEE30 pKa = 4.07ASSQIRR36 pKa = 11.84KK37 pKa = 9.39RR38 pKa = 11.84DD39 pKa = 3.18ISKK42 pKa = 10.51YY43 pKa = 9.21PIFVFARR50 pKa = 11.84QEE52 pKa = 4.02VPLGGLLINADD63 pKa = 3.92EE64 pKa = 5.63LNLEE68 pKa = 3.95WHH70 pKa = 6.2VFASYY75 pKa = 11.36LEE77 pKa = 4.91LFVQQGIVGQDD88 pKa = 3.44GIEE91 pKa = 4.28AFQSTYY97 pKa = 10.62RR98 pKa = 11.84DD99 pKa = 3.27ADD101 pKa = 3.84EE102 pKa = 4.42YY103 pKa = 11.61CCLFVLDD110 pKa = 4.67EE111 pKa = 4.36EE112 pKa = 4.73FTNFVYY118 pKa = 10.29IPYY121 pKa = 10.15PEE123 pKa = 4.46DD124 pKa = 3.11

Molecular weight: 14.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5D6VBM5|A0A5D6VBM5_9BACT Helix-turn-helix domain-containing protein OS=Hymenobacter sp. KIGAM108 OX=2606448 GN=FY528_04650 PE=4 SV=1
GG1 pKa = 7.36LRR3 pKa = 11.84LDD5 pKa = 3.56YY6 pKa = 11.26ARR8 pKa = 11.84THH10 pKa = 5.3GRR12 pKa = 11.84AVGGRR17 pKa = 11.84RR18 pKa = 11.84VRR20 pKa = 11.84GARR23 pKa = 11.84PLKK26 pKa = 10.24RR27 pKa = 11.84GPARR31 pKa = 11.84TLIGTLSVRR40 pKa = 11.84GLGAVQLLDD49 pKa = 3.39GALTKK54 pKa = 10.46RR55 pKa = 11.84RR56 pKa = 11.84FAFYY60 pKa = 10.35VARR63 pKa = 11.84CLAPALRR70 pKa = 11.84RR71 pKa = 11.84GDD73 pKa = 3.85VLVLDD78 pKa = 4.68NLPVHH83 pKa = 6.4HH84 pKa = 7.1LSGLAEE90 pKa = 3.96WLAKK94 pKa = 10.45CGIEE98 pKa = 4.82VVFLPPYY105 pKa = 10.57SPDD108 pKa = 3.32FSPIEE113 pKa = 4.01QAWSKK118 pKa = 11.18LKK120 pKa = 10.1TKK122 pKa = 10.43LRR124 pKa = 11.84AAAARR129 pKa = 11.84TLAEE133 pKa = 4.48LEE135 pKa = 4.31AALHH139 pKa = 6.22EE140 pKa = 4.41ALNWITSQDD149 pKa = 2.97ARR151 pKa = 11.84NWFDD155 pKa = 2.78HH156 pKa = 6.65CGYY159 pKa = 8.76HH160 pKa = 5.5TQTARR165 pKa = 3.19

Molecular weight: 18.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4114

0

4114

1408179

17

3286

342.3

37.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.574 ± 0.048

0.728 ± 0.012

4.743 ± 0.028

5.418 ± 0.04

4.053 ± 0.025

7.575 ± 0.043

2.074 ± 0.018

4.232 ± 0.033

3.874 ± 0.041

11.177 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.821 ± 0.018

3.616 ± 0.036

5.196 ± 0.025

4.963 ± 0.032

6.213 ± 0.036

5.485 ± 0.027

6.263 ± 0.047

7.201 ± 0.03

1.273 ± 0.018

3.52 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski