Taiyuan leafhopper virus
Average proteome isoelectric point is 6.5
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A455LU23|A0A455LU23_9VIRU Large structural protein OS=Taiyuan leafhopper virus OX=2482723 PE=4 SV=1
MM1 pKa = 7.61 ARR3 pKa = 11.84 QYY5 pKa = 11.58 LNAARR10 pKa = 11.84 GRR12 pKa = 11.84 DD13 pKa = 3.37 AGVSQLIAQLNQAVSEE29 pKa = 4.16 RR30 pKa = 11.84 SEE32 pKa = 4.36 DD33 pKa = 3.49 LTPITLVAGGDD44 pKa = 3.85 NIRR47 pKa = 11.84 DD48 pKa = 3.59 FAVAAVSGQPLDD60 pKa = 3.22 IRR62 pKa = 11.84 ARR64 pKa = 11.84 VPLAYY69 pKa = 9.72 KK70 pKa = 10.32 LATYY74 pKa = 10.07 FNSRR78 pKa = 11.84 LSNPTPDD85 pKa = 3.5 FLQARR90 pKa = 11.84 LWGDD94 pKa = 3.14 LHH96 pKa = 6.96 AVWFVGEE103 pKa = 4.28 GTNDD107 pKa = 3.26 QRR109 pKa = 11.84 ISANARR115 pKa = 11.84 LDD117 pKa = 3.83 SILIANPIEE126 pKa = 4.48 GPMMLQAFQKK136 pKa = 10.79 VYY138 pKa = 9.62 DD139 pKa = 3.91 TVRR142 pKa = 11.84 AAEE145 pKa = 4.24 GAGNIPWDD153 pKa = 3.72 EE154 pKa = 4.06 ASAVVPLAPDD164 pKa = 3.56 AQDD167 pKa = 4.18 LPNEE171 pKa = 4.32 FPLQKK176 pKa = 10.46 YY177 pKa = 8.78 RR178 pKa = 11.84 FFEE181 pKa = 4.49 ASAHH185 pKa = 6.06 AFDD188 pKa = 4.02 NTALVQVTAATQDD201 pKa = 3.29 ALASLRR207 pKa = 11.84 EE208 pKa = 4.09 INEE211 pKa = 3.89 NAVNVPPHH219 pKa = 6.19 NFVLNEE225 pKa = 3.85 AWPQNWARR233 pKa = 11.84 LSRR236 pKa = 11.84 ASRR239 pKa = 11.84 LACFEE244 pKa = 4.12 MLYY247 pKa = 8.74 HH248 pKa = 5.78 TNKK251 pKa = 9.82 VEE253 pKa = 3.68 WDD255 pKa = 3.71 SYY257 pKa = 11.2 LVASVAGVVFSICKK271 pKa = 9.41 SSGMTTAYY279 pKa = 10.49 LQTRR283 pKa = 11.84 VDD285 pKa = 4.45 RR286 pKa = 11.84 LVKK289 pKa = 10.25 DD290 pKa = 3.78 YY291 pKa = 11.39 PEE293 pKa = 6.44 LEE295 pKa = 4.32 LQDD298 pKa = 3.96 KK299 pKa = 11.22 LMMEE303 pKa = 4.85 FFMTFGKK310 pKa = 10.64 LYY312 pKa = 10.69 VPQNLDD318 pKa = 3.06 RR319 pKa = 11.84 TVIANYY325 pKa = 9.33 LQAVYY330 pKa = 10.52 GCLEE334 pKa = 4.34 EE335 pKa = 5.06 GPSRR339 pKa = 11.84 SIAWVIEE346 pKa = 3.65 QARR349 pKa = 11.84 GHH351 pKa = 6.44 NYY353 pKa = 7.61 THH355 pKa = 7.26 ALAFAEE361 pKa = 4.67 SCSKK365 pKa = 9.83 TKK367 pKa = 10.45 YY368 pKa = 10.67 LPIAYY373 pKa = 8.76 LANIRR378 pKa = 11.84 EE379 pKa = 4.27 DD380 pKa = 3.14 QWIGFIKK387 pKa = 10.25 VCYY390 pKa = 9.23 HH391 pKa = 5.58 VCRR394 pKa = 11.84 DD395 pKa = 3.35 PWGSLTGPAIPANNYY410 pKa = 9.47 PDD412 pKa = 3.47 IANIGAIMSKK422 pKa = 10.58 KK423 pKa = 9.63 LDD425 pKa = 3.4 KK426 pKa = 10.69 AARR429 pKa = 11.84 GYY431 pKa = 10.53 AGKK434 pKa = 10.34 FILGGEE440 pKa = 4.17 HH441 pKa = 6.57 TEE443 pKa = 4.23 PFCKK447 pKa = 10.08 AVADD451 pKa = 4.21 QIIEE455 pKa = 4.03 SHH457 pKa = 6.09 AASLEE462 pKa = 4.29 DD463 pKa = 4.02 EE464 pKa = 4.79 LSAANIIAALFPGIKK479 pKa = 8.75 MAVVGDD485 pKa = 3.29 NAYY488 pKa = 10.83 YY489 pKa = 10.31 YY490 pKa = 10.41 DD491 pKa = 4.11 EE492 pKa = 5.22 SGLPLEE498 pKa = 4.7 LTAEE502 pKa = 4.27 NARR505 pKa = 11.84 PVGGWEE511 pKa = 4.15 EE512 pKa = 4.23 EE513 pKa = 4.38 EE514 pKa = 4.47 EE515 pKa = 4.11 PAPAAGAAARR525 pKa = 11.84 PAAGFPGNVNRR536 pKa = 11.84 EE537 pKa = 3.42 VAYY540 pKa = 10.5 RR541 pKa = 11.84 GEE543 pKa = 4.1 QPLPQIAEE551 pKa = 4.07 DD552 pKa = 3.68 ALAEE556 pKa = 5.04 SRR558 pKa = 11.84 ATWANKK564 pKa = 9.43 AKK566 pKa = 10.21 NLPRR570 pKa = 11.84 NAVKK574 pKa = 8.09 MTMKK578 pKa = 10.65 DD579 pKa = 3.2 QVLLLEE585 pKa = 4.97 EE586 pKa = 4.47 YY587 pKa = 11.13 VDD589 pKa = 3.96 TDD591 pKa = 3.21 RR592 pKa = 11.84 HH593 pKa = 5.34 LSAIKK598 pKa = 9.11 TCCNLLLYY606 pKa = 10.45 ASKK609 pKa = 10.49 QSKK612 pKa = 10.11 LEE614 pKa = 4.16 PYY616 pKa = 10.24 GGAGDD621 pKa = 4.1 NIRR624 pKa = 11.84 VKK626 pKa = 10.57 NRR628 pKa = 11.84 YY629 pKa = 7.62 VASPAGLDD637 pKa = 3.16 NALNVWGLDD646 pKa = 3.66 GNDD649 pKa = 4.48 IIPDD653 pKa = 3.58 IPEE656 pKa = 4.35 DD657 pKa = 4.08 APNVPYY663 pKa = 10.88 NEE665 pKa = 3.72 NVMGNVALYY674 pKa = 9.84 ARR676 pKa = 11.84 PIRR679 pKa = 11.84 PIVQNRR685 pKa = 11.84 RR686 pKa = 11.84 GNAGLGGMLNWQPPQQNLL704 pKa = 3.1
Molecular weight: 77.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.028
IPC2_protein 5.105
IPC_protein 5.067
Toseland 4.991
ProMoST 5.194
Dawson 5.054
Bjellqvist 5.181
Wikipedia 4.914
Rodwell 4.965
Grimsley 4.914
Solomon 5.054
Lehninger 5.003
Nozaki 5.169
DTASelect 5.321
Thurlkill 5.003
EMBOSS 4.965
Sillero 5.245
Patrickios 3.897
IPC_peptide 5.054
IPC2_peptide 5.245
IPC2.peptide.svr19 5.185
Protein with the highest isoelectric point:
>tr|A0A455LK58|A0A455LK58_9VIRU Nucleocapsid protein OS=Taiyuan leafhopper virus OX=2482723 PE=4 SV=1
MM1 pKa = 7.72 EE2 pKa = 5.72 KK3 pKa = 9.4 FTSYY7 pKa = 10.98 SEE9 pKa = 4.05 QFVYY13 pKa = 10.62 LCQIYY18 pKa = 10.03 HH19 pKa = 7.28 LYY21 pKa = 9.91 LIAMSEE27 pKa = 4.39 SKK29 pKa = 10.72 QITSVTQARR38 pKa = 11.84 SAALSSGSVVPTNGNQQISRR58 pKa = 11.84 SEE60 pKa = 3.81 AMMALASDD68 pKa = 3.81 ISVIKK73 pKa = 10.36 SDD75 pKa = 3.38 VMVIKK80 pKa = 10.33 EE81 pKa = 4.55 ALTKK85 pKa = 8.67 IQEE88 pKa = 4.39 MIGGAMKK95 pKa = 8.4 TQPHH99 pKa = 7.03 APPPSYY105 pKa = 11.09 DD106 pKa = 3.09 SGIYY110 pKa = 9.63 PKK112 pKa = 10.28 LHH114 pKa = 6.14 RR115 pKa = 11.84 PAYY118 pKa = 10.32 LGGPRR123 pKa = 11.84 II124 pKa = 3.99
Molecular weight: 13.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.06
IPC2_protein 8.039
IPC_protein 8.287
Toseland 8.536
ProMoST 8.419
Dawson 8.785
Bjellqvist 8.712
Wikipedia 8.887
Rodwell 8.814
Grimsley 8.521
Solomon 9.092
Lehninger 9.063
Nozaki 8.712
DTASelect 8.653
Thurlkill 8.697
EMBOSS 8.96
Sillero 8.902
Patrickios 4.024
IPC_peptide 9.077
IPC2_peptide 7.644
IPC2.peptide.svr19 7.681
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
4604
124
2546
1151.0
130.55
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.777 ± 1.901
1.781 ± 0.199
5.061 ± 0.288
5.387 ± 0.39
3.844 ± 0.591
5.039 ± 0.399
3.215 ± 0.512
6.581 ± 0.427
3.975 ± 0.374
10.904 ± 0.754
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.824 ± 0.35
5.169 ± 0.519
4.93 ± 0.419
4.67 ± 0.223
5.864 ± 0.707
6.407 ± 1.314
6.103 ± 0.89
6.581 ± 0.301
1.586 ± 0.176
3.301 ± 0.362
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here