Lactococcus phage P162

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Nevevirus; Lactococcus virus P162

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 82 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|X4Y8A5|X4Y8A5_9CAUD Uncharacterized protein OS=Lactococcus phage P162 OX=1476889 GN=P162_0029 PE=4 SV=1
MM1 pKa = 8.18DD2 pKa = 5.95SILEE6 pKa = 3.94EE7 pKa = 4.72RR8 pKa = 11.84IQEE11 pKa = 4.34IIRR14 pKa = 11.84DD15 pKa = 3.7YY16 pKa = 10.92CIRR19 pKa = 11.84YY20 pKa = 9.13YY21 pKa = 11.4SNFNIPEE28 pKa = 4.47DD29 pKa = 3.62NLTNLLEE36 pKa = 4.3EE37 pKa = 4.98DD38 pKa = 4.23FFITDD43 pKa = 4.36EE44 pKa = 4.48IIGYY48 pKa = 9.52DD49 pKa = 3.04KK50 pKa = 11.04SLNVMIYY57 pKa = 10.7NNMDD61 pKa = 3.21YY62 pKa = 10.04TIHH65 pKa = 7.12VLEE68 pKa = 5.7CIDD71 pKa = 5.2DD72 pKa = 4.37IIEE75 pKa = 4.02RR76 pKa = 11.84SYY78 pKa = 11.49FSLDD82 pKa = 3.13NGG84 pKa = 3.9

Molecular weight: 10.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|X4YUW7|X4YUW7_9CAUD Uncharacterized protein OS=Lactococcus phage P162 OX=1476889 GN=P162_001 PE=4 SV=1
MM1 pKa = 7.72NKK3 pKa = 9.54RR4 pKa = 11.84QNISGKK10 pKa = 10.16AVYY13 pKa = 9.87SYY15 pKa = 11.34YY16 pKa = 11.01NKK18 pKa = 9.98NSRR21 pKa = 11.84SIRR24 pKa = 11.84ATMEE28 pKa = 3.81HH29 pKa = 5.76FHH31 pKa = 6.92IGSKK35 pKa = 9.38RR36 pKa = 11.84VKK38 pKa = 9.69TIVRR42 pKa = 11.84YY43 pKa = 9.7YY44 pKa = 10.26EE45 pKa = 4.15GADD48 pKa = 3.25VVFKK52 pKa = 8.75SHH54 pKa = 7.04RR55 pKa = 11.84GGRR58 pKa = 11.84KK59 pKa = 8.96SRR61 pKa = 11.84FDD63 pKa = 3.42RR64 pKa = 11.84ALLEE68 pKa = 4.29HH69 pKa = 7.01VEE71 pKa = 4.2DD72 pKa = 4.51MIKK75 pKa = 9.5ATHH78 pKa = 6.88IEE80 pKa = 4.12YY81 pKa = 10.33QGNGTKK87 pKa = 8.94VTVKK91 pKa = 9.62TYY93 pKa = 11.28KK94 pKa = 10.24NLSEE98 pKa = 3.96IAEE101 pKa = 4.23VLGVNHH107 pKa = 6.39KK108 pKa = 8.69TLANNLIEE116 pKa = 4.15YY117 pKa = 9.41RR118 pKa = 11.84KK119 pKa = 10.37NPLKK123 pKa = 10.82YY124 pKa = 9.95RR125 pKa = 3.8

Molecular weight: 14.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

82

0

82

17468

38

1692

213.0

24.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.509 ± 0.597

0.527 ± 0.088

6.772 ± 0.281

6.303 ± 0.468

3.687 ± 0.169

6.406 ± 0.492

1.603 ± 0.172

7.574 ± 0.324

8.163 ± 0.438

7.917 ± 0.328

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.885 ± 0.244

6.269 ± 0.381

3.332 ± 0.227

3.16 ± 0.15

3.715 ± 0.231

6.423 ± 0.3

6.721 ± 0.344

6.366 ± 0.17

1.219 ± 0.111

4.448 ± 0.419
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski