Wenzhou bivalvia virus 3

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1L3KFM4|A0A1L3KFM4_9VIRU RNA replicase OS=Wenzhou bivalvia virus 3 OX=1923555 PE=3 SV=1
MM1 pKa = 7.96DD2 pKa = 6.64CICADD7 pKa = 3.52QDD9 pKa = 3.85NVSAALTQGMFIGVNYY25 pKa = 9.9GSPRR29 pKa = 11.84SDD31 pKa = 3.93GIFTHH36 pKa = 6.52ARR38 pKa = 11.84KK39 pKa = 7.77TFQDD43 pKa = 3.09ALVNLFRR50 pKa = 11.84HH51 pKa = 6.16PDD53 pKa = 3.28IDD55 pKa = 4.48EE56 pKa = 4.41GAQKK60 pKa = 9.99IKK62 pKa = 10.7RR63 pKa = 11.84MSKK66 pKa = 9.83MIMLPSFAIGGGMLFYY82 pKa = 9.36TICKK86 pKa = 9.72LAGTYY91 pKa = 10.84ARR93 pKa = 11.84MYY95 pKa = 10.19LPRR98 pKa = 11.84RR99 pKa = 11.84TLPEE103 pKa = 3.43PTARR107 pKa = 11.84LDD109 pKa = 3.33VTFGKK114 pKa = 9.95ICLPKK119 pKa = 9.89VTVIDD124 pKa = 3.6TDD126 pKa = 3.4QHH128 pKa = 5.73LKK130 pKa = 9.33CWFWNGVFNWYY141 pKa = 8.55WRR143 pKa = 11.84VDD145 pKa = 3.06CWFNHH150 pKa = 4.54QAASILRR157 pKa = 11.84LLHH160 pKa = 6.14LVSNEE165 pKa = 3.24RR166 pKa = 11.84HH167 pKa = 4.86LWFIKK172 pKa = 10.08GLNVRR177 pKa = 11.84LMNSAYY183 pKa = 10.05EE184 pKa = 4.08DD185 pKa = 3.65LRR187 pKa = 11.84SLFIHH192 pKa = 7.0RR193 pKa = 11.84GNGFDD198 pKa = 3.36YY199 pKa = 10.58HH200 pKa = 6.81KK201 pKa = 10.63RR202 pKa = 11.84PIMKK206 pKa = 9.91RR207 pKa = 11.84RR208 pKa = 11.84LLDD211 pKa = 3.4VEE213 pKa = 4.89PIFAKK218 pKa = 10.63VGGAHH223 pKa = 5.68THH225 pKa = 6.83PEE227 pKa = 3.84AASEE231 pKa = 4.06RR232 pKa = 11.84NAMGPYY238 pKa = 9.76FKK240 pKa = 10.62RR241 pKa = 11.84LAAMMGRR248 pKa = 11.84DD249 pKa = 3.44LFMYY253 pKa = 10.18SLSNADD259 pKa = 2.83IKK261 pKa = 11.46ADD263 pKa = 3.44TRR265 pKa = 11.84GNKK268 pKa = 7.58TMYY271 pKa = 8.85WPKK274 pKa = 11.01DD275 pKa = 3.22LLVPHH280 pKa = 6.76TAALPKK286 pKa = 8.32EE287 pKa = 3.96TDD289 pKa = 3.22MIGVVDD295 pKa = 4.57CDD297 pKa = 3.5WYY299 pKa = 11.74LDD301 pKa = 3.82MNDD304 pKa = 3.52LLTRR308 pKa = 11.84YY309 pKa = 9.15VVTWAIYY316 pKa = 9.82GLNPTEE322 pKa = 4.49PGGKK326 pKa = 9.13VKK328 pKa = 10.97NGDD331 pKa = 3.19FTFINNVLHH340 pKa = 5.95YY341 pKa = 10.23SITGGANYY349 pKa = 9.88KK350 pKa = 10.34HH351 pKa = 6.37EE352 pKa = 4.18LWDD355 pKa = 3.53WNRR358 pKa = 11.84EE359 pKa = 4.2TICASRR365 pKa = 11.84YY366 pKa = 9.61EE367 pKa = 4.24DD368 pKa = 4.16GEE370 pKa = 4.3WYY372 pKa = 10.29AAVYY376 pKa = 9.17MVEE379 pKa = 4.04QKK381 pKa = 9.67TISDD385 pKa = 3.49HH386 pKa = 6.01RR387 pKa = 11.84VVVLLEE393 pKa = 4.86PIFKK397 pKa = 10.55CKK399 pKa = 10.14GLAAQLLIEE408 pKa = 4.59QSHH411 pKa = 5.84TPNAQMGRR419 pKa = 11.84LVRR422 pKa = 11.84MKK424 pKa = 9.63PQPNPDD430 pKa = 2.9VNYY433 pKa = 11.02NIIEE437 pKa = 4.27CLKK440 pKa = 10.22RR441 pKa = 11.84TDD443 pKa = 4.36DD444 pKa = 3.64KK445 pKa = 11.92AGGNVVRR452 pKa = 11.84TVSVSPVGSRR462 pKa = 11.84KK463 pKa = 9.64SATVTAEE470 pKa = 3.41VFAAMRR476 pKa = 11.84ATVLSSKK483 pKa = 10.63QPITAAALGSHH494 pKa = 6.77MGTGPNVRR502 pKa = 11.84RR503 pKa = 11.84DD504 pKa = 3.35AEE506 pKa = 4.1MFRR509 pKa = 11.84LYY511 pKa = 10.34FQQTEE516 pKa = 3.74KK517 pKa = 11.02DD518 pKa = 3.79ANPEE522 pKa = 3.9SLVISNEE529 pKa = 4.09NACKK533 pKa = 10.37GYY535 pKa = 8.39TFNPQHH541 pKa = 6.98SIQEE545 pKa = 4.16INSAAMKK552 pKa = 8.93QKK554 pKa = 10.43CKK556 pKa = 10.28AVCSPFVGSPALTPMSSLASDD577 pKa = 3.49QVGVANRR584 pKa = 11.84ITKK587 pKa = 9.3LQHH590 pKa = 4.25NTEE593 pKa = 4.13LKK595 pKa = 10.73GDD597 pKa = 3.58QFLYY601 pKa = 10.85KK602 pKa = 10.85VMGEE606 pKa = 3.96FAEE609 pKa = 5.23LIVPEE614 pKa = 4.8HH615 pKa = 6.66KK616 pKa = 10.06HH617 pKa = 5.74HH618 pKa = 5.7QLKK621 pKa = 10.03PKK623 pKa = 9.73YY624 pKa = 9.46VEE626 pKa = 4.5DD627 pKa = 3.92VFVQQDD633 pKa = 3.25RR634 pKa = 11.84PSQRR638 pKa = 11.84NTLHH642 pKa = 7.01RR643 pKa = 11.84GDD645 pKa = 4.01NYY647 pKa = 10.75GDD649 pKa = 3.81YY650 pKa = 9.29MRR652 pKa = 11.84HH653 pKa = 6.66DD654 pKa = 4.38IIEE657 pKa = 4.32TFQKK661 pKa = 10.85NEE663 pKa = 4.03VYY665 pKa = 10.67GKK667 pKa = 10.3LADD670 pKa = 4.02PRR672 pKa = 11.84TISQVDD678 pKa = 3.7PRR680 pKa = 11.84TKK682 pKa = 9.78LAYY685 pKa = 10.74SMFYY689 pKa = 9.93MVISEE694 pKa = 5.34EE695 pKa = 3.97ILKK698 pKa = 8.26PQPWYY703 pKa = 9.98TFGKK707 pKa = 7.76TPKK710 pKa = 10.21EE711 pKa = 3.63MAEE714 pKa = 3.85RR715 pKa = 11.84VAYY718 pKa = 9.54IATGADD724 pKa = 3.52SVVMGDD730 pKa = 4.11FSRR733 pKa = 11.84MDD735 pKa = 3.25ATISNLGRR743 pKa = 11.84HH744 pKa = 5.44LTTSIMMRR752 pKa = 11.84AFHH755 pKa = 6.62PSYY758 pKa = 10.75HH759 pKa = 6.86EE760 pKa = 4.02EE761 pKa = 4.08LQTLLQRR768 pKa = 11.84QCHH771 pKa = 4.71RR772 pKa = 11.84TCFTRR777 pKa = 11.84FGVKK781 pKa = 8.24YY782 pKa = 8.86TSGTSRR788 pKa = 11.84LSGSPEE794 pKa = 3.82TSGHH798 pKa = 4.69NTIEE802 pKa = 4.21NAFIAYY808 pKa = 8.98LALRR812 pKa = 11.84MTKK815 pKa = 10.51DD816 pKa = 3.09EE817 pKa = 4.69DD818 pKa = 4.29GNWIEE823 pKa = 4.08RR824 pKa = 11.84DD825 pKa = 2.92RR826 pKa = 11.84AYY828 pKa = 10.76RR829 pKa = 11.84ILCTRR834 pKa = 11.84TEE836 pKa = 4.07FGGDD840 pKa = 3.5DD841 pKa = 3.98SIMFSLGPGITEE853 pKa = 3.7EE854 pKa = 4.89TYY856 pKa = 10.9CKK858 pKa = 10.12AASKK862 pKa = 9.84MGLKK866 pKa = 8.75ATCQVLQRR874 pKa = 11.84WDD876 pKa = 3.44LGVNFLARR884 pKa = 11.84FFSRR888 pKa = 11.84HH889 pKa = 4.24VWAGSPNSTCDD900 pKa = 3.02IRR902 pKa = 11.84RR903 pKa = 11.84QATKK907 pKa = 10.76LHH909 pKa = 6.1TTTQMDD915 pKa = 4.02GDD917 pKa = 4.55PYY919 pKa = 11.48DD920 pKa = 3.61VLDD923 pKa = 4.61RR924 pKa = 11.84KK925 pKa = 9.44MLSYY929 pKa = 11.58ALTDD933 pKa = 3.98PNTPLIGDD941 pKa = 5.26LATTWCRR948 pKa = 11.84LRR950 pKa = 11.84GIPLPTRR957 pKa = 11.84ALDD960 pKa = 3.88PDD962 pKa = 3.99LTRR965 pKa = 11.84VKK967 pKa = 10.61DD968 pKa = 4.01VSWWAALYY976 pKa = 10.82DD977 pKa = 4.61KK978 pKa = 10.79DD979 pKa = 4.57VQFPNDD985 pKa = 3.42NEE987 pKa = 3.94VDD989 pKa = 3.7NFHH992 pKa = 7.29IDD994 pKa = 3.72YY995 pKa = 10.71LRR997 pKa = 11.84EE998 pKa = 4.09AYY1000 pKa = 9.59PDD1002 pKa = 3.13IEE1004 pKa = 4.04YY1005 pKa = 10.92VAFRR1009 pKa = 11.84EE1010 pKa = 4.13WLDD1013 pKa = 3.62DD1014 pKa = 3.56VKK1016 pKa = 10.53TPEE1019 pKa = 3.97EE1020 pKa = 4.42FMRR1023 pKa = 11.84SPPWTAIEE1031 pKa = 4.38EE1032 pKa = 4.52IKK1034 pKa = 10.36PHH1036 pKa = 6.77PDD1038 pKa = 3.04EE1039 pKa = 5.52DD1040 pKa = 3.9VLIIDD1045 pKa = 4.84GDD1047 pKa = 3.72DD1048 pKa = 3.44AYY1050 pKa = 10.04IIDD1053 pKa = 3.83SEE1055 pKa = 4.5KK1056 pKa = 9.82TVEE1059 pKa = 4.09EE1060 pKa = 4.4EE1061 pKa = 3.87KK1062 pKa = 10.86HH1063 pKa = 5.39GTSSLDD1069 pKa = 3.13VDD1071 pKa = 3.96SPKK1074 pKa = 10.38PVSVRR1079 pKa = 11.84NRR1081 pKa = 11.84PKK1083 pKa = 10.24RR1084 pKa = 11.84DD1085 pKa = 3.47PKK1087 pKa = 10.46HH1088 pKa = 6.13AQASNLGKK1096 pKa = 7.91QTKK1099 pKa = 9.51PPGRR1103 pKa = 11.84VVRR1106 pKa = 11.84AHH1108 pKa = 5.03TTKK1111 pKa = 10.76LPPSHH1116 pKa = 6.68GKK1118 pKa = 7.54TPTWKK1123 pKa = 9.59PVSLSGRR1130 pKa = 11.84VALGSRR1136 pKa = 11.84SFSGRR1141 pKa = 11.84GRR1143 pKa = 11.84RR1144 pKa = 11.84PPSNGQRR1151 pKa = 11.84PTAGKK1156 pKa = 10.25GRR1158 pKa = 11.84SAPGKK1163 pKa = 10.27VSDD1166 pKa = 4.28SAA1168 pKa = 3.82

Molecular weight:
132.18 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1L3KFM4|A0A1L3KFM4_9VIRU RNA replicase OS=Wenzhou bivalvia virus 3 OX=1923555 PE=3 SV=1
MM1 pKa = 7.65ALNHH5 pKa = 6.73DD6 pKa = 3.69SRR8 pKa = 11.84RR9 pKa = 11.84FLEE12 pKa = 4.64AATCAPDD19 pKa = 4.69NDD21 pKa = 4.14LQPRR25 pKa = 11.84VPDD28 pKa = 3.8GSGTRR33 pKa = 11.84SVVQKK38 pKa = 9.81FVKK41 pKa = 9.89QDD43 pKa = 3.74NISVKK48 pKa = 10.15PGYY51 pKa = 6.33TTIVVCTPTLPMAYY65 pKa = 8.9YY66 pKa = 9.67TVTYY70 pKa = 10.68SNTTGFVPTAGNYY83 pKa = 8.22PPLSSIGAEE92 pKa = 3.9YY93 pKa = 10.74QEE95 pKa = 4.29AHH97 pKa = 6.0TEE99 pKa = 4.09FPQYY103 pKa = 11.01FNTVNNSSGVSNTASIDD120 pKa = 3.3AARR123 pKa = 11.84VISRR127 pKa = 11.84SAEE130 pKa = 3.9LASTTNALNQYY141 pKa = 8.03GTITTFKK148 pKa = 11.03SPFALCLQPEE158 pKa = 4.31IVGTGILGPTSYY170 pKa = 11.01NITGARR176 pKa = 11.84AIVYY180 pKa = 8.67EE181 pKa = 4.41GAAIGSTVGFVKK193 pKa = 10.69DD194 pKa = 3.33GAYY197 pKa = 9.59SVSMNRR203 pKa = 11.84LGGTGVFPYY212 pKa = 10.65TEE214 pKa = 5.67FIDD217 pKa = 4.38NMADD221 pKa = 3.47NEE223 pKa = 4.59TVPSSILSGPSSLEE237 pKa = 3.37ILPWKK242 pKa = 9.38GAPFFWDD249 pKa = 3.28NNFDD253 pKa = 4.23TICFKK258 pKa = 10.83IFVPDD263 pKa = 4.34GVPSAQTFILKK274 pKa = 9.67NWATVEE280 pKa = 3.86MRR282 pKa = 11.84TVQGSWLNGFAQPAPPLDD300 pKa = 3.29ARR302 pKa = 11.84AFRR305 pKa = 11.84LYY307 pKa = 11.01GAIQEE312 pKa = 4.4KK313 pKa = 10.74LPVSVPSKK321 pKa = 10.3DD322 pKa = 4.49NPNFWNTVLGLVKK335 pKa = 9.84PLSGFASMMPGPIGAVAKK353 pKa = 10.06GVHH356 pKa = 5.93AVSSVLSPNSQHH368 pKa = 7.19RR369 pKa = 11.84EE370 pKa = 3.71AKK372 pKa = 10.65ANLGPFKK379 pKa = 10.71IKK381 pKa = 9.24LTNRR385 pKa = 11.84NKK387 pKa = 10.18PKK389 pKa = 10.29KK390 pKa = 9.61KK391 pKa = 9.84KK392 pKa = 9.43KK393 pKa = 10.14KK394 pKa = 9.23KK395 pKa = 8.76GNRR398 pKa = 11.84PRR400 pKa = 11.84SVVGNQRR407 pKa = 11.84LIRR410 pKa = 4.33

Molecular weight:
44.24 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1578

410

1168

789.0

88.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.605 ± 0.468

1.521 ± 0.274

5.83 ± 1.213

4.373 ± 0.603

4.183 ± 0.472

6.844 ± 0.605

2.535 ± 0.783

4.943 ± 0.09

5.767 ± 0.079

7.288 ± 0.475

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.915 ± 0.606

4.88 ± 0.979

6.401 ± 0.828

3.232 ± 0.031

6.084 ± 0.973

6.717 ± 0.913

7.034 ± 0.387

6.654 ± 0.7

1.711 ± 0.247

3.485 ± 0.158

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski