Wenzhou bivalvia virus 3
Average proteome isoelectric point is 8.16
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KFM4|A0A1L3KFM4_9VIRU RNA replicase OS=Wenzhou bivalvia virus 3 OX=1923555 PE=3 SV=1
MM1 pKa = 7.96 DD2 pKa = 6.64 CICADD7 pKa = 3.52 QDD9 pKa = 3.85 NVSAALTQGMFIGVNYY25 pKa = 9.9 GSPRR29 pKa = 11.84 SDD31 pKa = 3.93 GIFTHH36 pKa = 6.52 ARR38 pKa = 11.84 KK39 pKa = 7.77 TFQDD43 pKa = 3.09 ALVNLFRR50 pKa = 11.84 HH51 pKa = 6.16 PDD53 pKa = 3.28 IDD55 pKa = 4.48 EE56 pKa = 4.41 GAQKK60 pKa = 9.99 IKK62 pKa = 10.7 RR63 pKa = 11.84 MSKK66 pKa = 9.83 MIMLPSFAIGGGMLFYY82 pKa = 9.36 TICKK86 pKa = 9.72 LAGTYY91 pKa = 10.84 ARR93 pKa = 11.84 MYY95 pKa = 10.19 LPRR98 pKa = 11.84 RR99 pKa = 11.84 TLPEE103 pKa = 3.43 PTARR107 pKa = 11.84 LDD109 pKa = 3.33 VTFGKK114 pKa = 9.95 ICLPKK119 pKa = 9.89 VTVIDD124 pKa = 3.6 TDD126 pKa = 3.4 QHH128 pKa = 5.73 LKK130 pKa = 9.33 CWFWNGVFNWYY141 pKa = 8.55 WRR143 pKa = 11.84 VDD145 pKa = 3.06 CWFNHH150 pKa = 4.54 QAASILRR157 pKa = 11.84 LLHH160 pKa = 6.14 LVSNEE165 pKa = 3.24 RR166 pKa = 11.84 HH167 pKa = 4.86 LWFIKK172 pKa = 10.08 GLNVRR177 pKa = 11.84 LMNSAYY183 pKa = 10.05 EE184 pKa = 4.08 DD185 pKa = 3.65 LRR187 pKa = 11.84 SLFIHH192 pKa = 7.0 RR193 pKa = 11.84 GNGFDD198 pKa = 3.36 YY199 pKa = 10.58 HH200 pKa = 6.81 KK201 pKa = 10.63 RR202 pKa = 11.84 PIMKK206 pKa = 9.91 RR207 pKa = 11.84 RR208 pKa = 11.84 LLDD211 pKa = 3.4 VEE213 pKa = 4.89 PIFAKK218 pKa = 10.63 VGGAHH223 pKa = 5.68 THH225 pKa = 6.83 PEE227 pKa = 3.84 AASEE231 pKa = 4.06 RR232 pKa = 11.84 NAMGPYY238 pKa = 9.76 FKK240 pKa = 10.62 RR241 pKa = 11.84 LAAMMGRR248 pKa = 11.84 DD249 pKa = 3.44 LFMYY253 pKa = 10.18 SLSNADD259 pKa = 2.83 IKK261 pKa = 11.46 ADD263 pKa = 3.44 TRR265 pKa = 11.84 GNKK268 pKa = 7.58 TMYY271 pKa = 8.85 WPKK274 pKa = 11.01 DD275 pKa = 3.22 LLVPHH280 pKa = 6.76 TAALPKK286 pKa = 8.32 EE287 pKa = 3.96 TDD289 pKa = 3.22 MIGVVDD295 pKa = 4.57 CDD297 pKa = 3.5 WYY299 pKa = 11.74 LDD301 pKa = 3.82 MNDD304 pKa = 3.52 LLTRR308 pKa = 11.84 YY309 pKa = 9.15 VVTWAIYY316 pKa = 9.82 GLNPTEE322 pKa = 4.49 PGGKK326 pKa = 9.13 VKK328 pKa = 10.97 NGDD331 pKa = 3.19 FTFINNVLHH340 pKa = 5.95 YY341 pKa = 10.23 SITGGANYY349 pKa = 9.88 KK350 pKa = 10.34 HH351 pKa = 6.37 EE352 pKa = 4.18 LWDD355 pKa = 3.53 WNRR358 pKa = 11.84 EE359 pKa = 4.2 TICASRR365 pKa = 11.84 YY366 pKa = 9.61 EE367 pKa = 4.24 DD368 pKa = 4.16 GEE370 pKa = 4.3 WYY372 pKa = 10.29 AAVYY376 pKa = 9.17 MVEE379 pKa = 4.04 QKK381 pKa = 9.67 TISDD385 pKa = 3.49 HH386 pKa = 6.01 RR387 pKa = 11.84 VVVLLEE393 pKa = 4.86 PIFKK397 pKa = 10.55 CKK399 pKa = 10.14 GLAAQLLIEE408 pKa = 4.59 QSHH411 pKa = 5.84 TPNAQMGRR419 pKa = 11.84 LVRR422 pKa = 11.84 MKK424 pKa = 9.63 PQPNPDD430 pKa = 2.9 VNYY433 pKa = 11.02 NIIEE437 pKa = 4.27 CLKK440 pKa = 10.22 RR441 pKa = 11.84 TDD443 pKa = 4.36 DD444 pKa = 3.64 KK445 pKa = 11.92 AGGNVVRR452 pKa = 11.84 TVSVSPVGSRR462 pKa = 11.84 KK463 pKa = 9.64 SATVTAEE470 pKa = 3.41 VFAAMRR476 pKa = 11.84 ATVLSSKK483 pKa = 10.63 QPITAAALGSHH494 pKa = 6.77 MGTGPNVRR502 pKa = 11.84 RR503 pKa = 11.84 DD504 pKa = 3.35 AEE506 pKa = 4.1 MFRR509 pKa = 11.84 LYY511 pKa = 10.34 FQQTEE516 pKa = 3.74 KK517 pKa = 11.02 DD518 pKa = 3.79 ANPEE522 pKa = 3.9 SLVISNEE529 pKa = 4.09 NACKK533 pKa = 10.37 GYY535 pKa = 8.39 TFNPQHH541 pKa = 6.98 SIQEE545 pKa = 4.16 INSAAMKK552 pKa = 8.93 QKK554 pKa = 10.43 CKK556 pKa = 10.28 AVCSPFVGSPALTPMSSLASDD577 pKa = 3.49 QVGVANRR584 pKa = 11.84 ITKK587 pKa = 9.3 LQHH590 pKa = 4.25 NTEE593 pKa = 4.13 LKK595 pKa = 10.73 GDD597 pKa = 3.58 QFLYY601 pKa = 10.85 KK602 pKa = 10.85 VMGEE606 pKa = 3.96 FAEE609 pKa = 5.23 LIVPEE614 pKa = 4.8 HH615 pKa = 6.66 KK616 pKa = 10.06 HH617 pKa = 5.74 HH618 pKa = 5.7 QLKK621 pKa = 10.03 PKK623 pKa = 9.73 YY624 pKa = 9.46 VEE626 pKa = 4.5 DD627 pKa = 3.92 VFVQQDD633 pKa = 3.25 RR634 pKa = 11.84 PSQRR638 pKa = 11.84 NTLHH642 pKa = 7.01 RR643 pKa = 11.84 GDD645 pKa = 4.01 NYY647 pKa = 10.75 GDD649 pKa = 3.81 YY650 pKa = 9.29 MRR652 pKa = 11.84 HH653 pKa = 6.66 DD654 pKa = 4.38 IIEE657 pKa = 4.32 TFQKK661 pKa = 10.85 NEE663 pKa = 4.03 VYY665 pKa = 10.67 GKK667 pKa = 10.3 LADD670 pKa = 4.02 PRR672 pKa = 11.84 TISQVDD678 pKa = 3.7 PRR680 pKa = 11.84 TKK682 pKa = 9.78 LAYY685 pKa = 10.74 SMFYY689 pKa = 9.93 MVISEE694 pKa = 5.34 EE695 pKa = 3.97 ILKK698 pKa = 8.26 PQPWYY703 pKa = 9.98 TFGKK707 pKa = 7.76 TPKK710 pKa = 10.21 EE711 pKa = 3.63 MAEE714 pKa = 3.85 RR715 pKa = 11.84 VAYY718 pKa = 9.54 IATGADD724 pKa = 3.52 SVVMGDD730 pKa = 4.11 FSRR733 pKa = 11.84 MDD735 pKa = 3.25 ATISNLGRR743 pKa = 11.84 HH744 pKa = 5.44 LTTSIMMRR752 pKa = 11.84 AFHH755 pKa = 6.62 PSYY758 pKa = 10.75 HH759 pKa = 6.86 EE760 pKa = 4.02 EE761 pKa = 4.08 LQTLLQRR768 pKa = 11.84 QCHH771 pKa = 4.71 RR772 pKa = 11.84 TCFTRR777 pKa = 11.84 FGVKK781 pKa = 8.24 YY782 pKa = 8.86 TSGTSRR788 pKa = 11.84 LSGSPEE794 pKa = 3.82 TSGHH798 pKa = 4.69 NTIEE802 pKa = 4.21 NAFIAYY808 pKa = 8.98 LALRR812 pKa = 11.84 MTKK815 pKa = 10.51 DD816 pKa = 3.09 EE817 pKa = 4.69 DD818 pKa = 4.29 GNWIEE823 pKa = 4.08 RR824 pKa = 11.84 DD825 pKa = 2.92 RR826 pKa = 11.84 AYY828 pKa = 10.76 RR829 pKa = 11.84 ILCTRR834 pKa = 11.84 TEE836 pKa = 4.07 FGGDD840 pKa = 3.5 DD841 pKa = 3.98 SIMFSLGPGITEE853 pKa = 3.7 EE854 pKa = 4.89 TYY856 pKa = 10.9 CKK858 pKa = 10.12 AASKK862 pKa = 9.84 MGLKK866 pKa = 8.75 ATCQVLQRR874 pKa = 11.84 WDD876 pKa = 3.44 LGVNFLARR884 pKa = 11.84 FFSRR888 pKa = 11.84 HH889 pKa = 4.24 VWAGSPNSTCDD900 pKa = 3.02 IRR902 pKa = 11.84 RR903 pKa = 11.84 QATKK907 pKa = 10.76 LHH909 pKa = 6.1 TTTQMDD915 pKa = 4.02 GDD917 pKa = 4.55 PYY919 pKa = 11.48 DD920 pKa = 3.61 VLDD923 pKa = 4.61 RR924 pKa = 11.84 KK925 pKa = 9.44 MLSYY929 pKa = 11.58 ALTDD933 pKa = 3.98 PNTPLIGDD941 pKa = 5.26 LATTWCRR948 pKa = 11.84 LRR950 pKa = 11.84 GIPLPTRR957 pKa = 11.84 ALDD960 pKa = 3.88 PDD962 pKa = 3.99 LTRR965 pKa = 11.84 VKK967 pKa = 10.61 DD968 pKa = 4.01 VSWWAALYY976 pKa = 10.82 DD977 pKa = 4.61 KK978 pKa = 10.79 DD979 pKa = 4.57 VQFPNDD985 pKa = 3.42 NEE987 pKa = 3.94 VDD989 pKa = 3.7 NFHH992 pKa = 7.29 IDD994 pKa = 3.72 YY995 pKa = 10.71 LRR997 pKa = 11.84 EE998 pKa = 4.09 AYY1000 pKa = 9.59 PDD1002 pKa = 3.13 IEE1004 pKa = 4.04 YY1005 pKa = 10.92 VAFRR1009 pKa = 11.84 EE1010 pKa = 4.13 WLDD1013 pKa = 3.62 DD1014 pKa = 3.56 VKK1016 pKa = 10.53 TPEE1019 pKa = 3.97 EE1020 pKa = 4.42 FMRR1023 pKa = 11.84 SPPWTAIEE1031 pKa = 4.38 EE1032 pKa = 4.52 IKK1034 pKa = 10.36 PHH1036 pKa = 6.77 PDD1038 pKa = 3.04 EE1039 pKa = 5.52 DD1040 pKa = 3.9 VLIIDD1045 pKa = 4.84 GDD1047 pKa = 3.72 DD1048 pKa = 3.44 AYY1050 pKa = 10.04 IIDD1053 pKa = 3.83 SEE1055 pKa = 4.5 KK1056 pKa = 9.82 TVEE1059 pKa = 4.09 EE1060 pKa = 4.4 EE1061 pKa = 3.87 KK1062 pKa = 10.86 HH1063 pKa = 5.39 GTSSLDD1069 pKa = 3.13 VDD1071 pKa = 3.96 SPKK1074 pKa = 10.38 PVSVRR1079 pKa = 11.84 NRR1081 pKa = 11.84 PKK1083 pKa = 10.24 RR1084 pKa = 11.84 DD1085 pKa = 3.47 PKK1087 pKa = 10.46 HH1088 pKa = 6.13 AQASNLGKK1096 pKa = 7.91 QTKK1099 pKa = 9.51 PPGRR1103 pKa = 11.84 VVRR1106 pKa = 11.84 AHH1108 pKa = 5.03 TTKK1111 pKa = 10.76 LPPSHH1116 pKa = 6.68 GKK1118 pKa = 7.54 TPTWKK1123 pKa = 9.59 PVSLSGRR1130 pKa = 11.84 VALGSRR1136 pKa = 11.84 SFSGRR1141 pKa = 11.84 GRR1143 pKa = 11.84 RR1144 pKa = 11.84 PPSNGQRR1151 pKa = 11.84 PTAGKK1156 pKa = 10.25 GRR1158 pKa = 11.84 SAPGKK1163 pKa = 10.27 VSDD1166 pKa = 4.28 SAA1168 pKa = 3.82
Molecular weight: 132.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.454
IPC2_protein 7.834
IPC_protein 7.629
Toseland 7.41
ProMoST 8.244
Dawson 8.404
Bjellqvist 8.712
Wikipedia 8.346
Rodwell 8.448
Grimsley 7.497
Solomon 8.434
Lehninger 8.448
Nozaki 8.858
DTASelect 8.463
Thurlkill 8.521
EMBOSS 8.595
Sillero 8.814
Patrickios 4.444
IPC_peptide 8.434
IPC2_peptide 7.571
IPC2.peptide.svr19 7.78
Protein with the highest isoelectric point:
>tr|A0A1L3KFM4|A0A1L3KFM4_9VIRU RNA replicase OS=Wenzhou bivalvia virus 3 OX=1923555 PE=3 SV=1
MM1 pKa = 7.65 ALNHH5 pKa = 6.73 DD6 pKa = 3.69 SRR8 pKa = 11.84 RR9 pKa = 11.84 FLEE12 pKa = 4.64 AATCAPDD19 pKa = 4.69 NDD21 pKa = 4.14 LQPRR25 pKa = 11.84 VPDD28 pKa = 3.8 GSGTRR33 pKa = 11.84 SVVQKK38 pKa = 9.81 FVKK41 pKa = 9.89 QDD43 pKa = 3.74 NISVKK48 pKa = 10.15 PGYY51 pKa = 6.33 TTIVVCTPTLPMAYY65 pKa = 8.9 YY66 pKa = 9.67 TVTYY70 pKa = 10.68 SNTTGFVPTAGNYY83 pKa = 8.22 PPLSSIGAEE92 pKa = 3.9 YY93 pKa = 10.74 QEE95 pKa = 4.29 AHH97 pKa = 6.0 TEE99 pKa = 4.09 FPQYY103 pKa = 11.01 FNTVNNSSGVSNTASIDD120 pKa = 3.3 AARR123 pKa = 11.84 VISRR127 pKa = 11.84 SAEE130 pKa = 3.9 LASTTNALNQYY141 pKa = 8.03 GTITTFKK148 pKa = 11.03 SPFALCLQPEE158 pKa = 4.31 IVGTGILGPTSYY170 pKa = 11.01 NITGARR176 pKa = 11.84 AIVYY180 pKa = 8.67 EE181 pKa = 4.41 GAAIGSTVGFVKK193 pKa = 10.69 DD194 pKa = 3.33 GAYY197 pKa = 9.59 SVSMNRR203 pKa = 11.84 LGGTGVFPYY212 pKa = 10.65 TEE214 pKa = 5.67 FIDD217 pKa = 4.38 NMADD221 pKa = 3.47 NEE223 pKa = 4.59 TVPSSILSGPSSLEE237 pKa = 3.37 ILPWKK242 pKa = 9.38 GAPFFWDD249 pKa = 3.28 NNFDD253 pKa = 4.23 TICFKK258 pKa = 10.83 IFVPDD263 pKa = 4.34 GVPSAQTFILKK274 pKa = 9.67 NWATVEE280 pKa = 3.86 MRR282 pKa = 11.84 TVQGSWLNGFAQPAPPLDD300 pKa = 3.29 ARR302 pKa = 11.84 AFRR305 pKa = 11.84 LYY307 pKa = 11.01 GAIQEE312 pKa = 4.4 KK313 pKa = 10.74 LPVSVPSKK321 pKa = 10.3 DD322 pKa = 4.49 NPNFWNTVLGLVKK335 pKa = 9.84 PLSGFASMMPGPIGAVAKK353 pKa = 10.06 GVHH356 pKa = 5.93 AVSSVLSPNSQHH368 pKa = 7.19 RR369 pKa = 11.84 EE370 pKa = 3.71 AKK372 pKa = 10.65 ANLGPFKK379 pKa = 10.71 IKK381 pKa = 9.24 LTNRR385 pKa = 11.84 NKK387 pKa = 10.18 PKK389 pKa = 10.29 KK390 pKa = 9.61 KK391 pKa = 9.84 KK392 pKa = 9.43 KK393 pKa = 10.14 KK394 pKa = 9.23 KK395 pKa = 8.76 GNRR398 pKa = 11.84 PRR400 pKa = 11.84 SVVGNQRR407 pKa = 11.84 LIRR410 pKa = 4.33
Molecular weight: 44.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.867
IPC2_protein 8.931
IPC_protein 8.843
Toseland 9.502
ProMoST 9.355
Dawson 9.794
Bjellqvist 9.516
Wikipedia 9.97
Rodwell 10.087
Grimsley 9.867
Solomon 9.823
Lehninger 9.78
Nozaki 9.604
DTASelect 9.487
Thurlkill 9.633
EMBOSS 9.94
Sillero 9.736
Patrickios 4.978
IPC_peptide 9.809
IPC2_peptide 8.141
IPC2.peptide.svr19 7.941
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1578
410
1168
789.0
88.21
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.605 ± 0.468
1.521 ± 0.274
5.83 ± 1.213
4.373 ± 0.603
4.183 ± 0.472
6.844 ± 0.605
2.535 ± 0.783
4.943 ± 0.09
5.767 ± 0.079
7.288 ± 0.475
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.915 ± 0.606
4.88 ± 0.979
6.401 ± 0.828
3.232 ± 0.031
6.084 ± 0.973
6.717 ± 0.913
7.034 ± 0.387
6.654 ± 0.7
1.711 ± 0.247
3.485 ± 0.158
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here