Burkholderia virus BcepF1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Bcepfunavirus

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 127 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A1Z014|A1Z014_9CAUD Uncharacterized protein OS=Burkholderia virus BcepF1 OX=417280 GN=BcepF1.110 PE=4 SV=1
MM1 pKa = 7.38FKK3 pKa = 10.27IPLIQVPNQAISFNVDD19 pKa = 2.61SAYY22 pKa = 7.2WTIRR26 pKa = 11.84IFQAIDD32 pKa = 3.15HH33 pKa = 6.21MCADD37 pKa = 4.35VSRR40 pKa = 11.84GGSPIARR47 pKa = 11.84GIRR50 pKa = 11.84CFSGIPLMPYY60 pKa = 9.88EE61 pKa = 4.43YY62 pKa = 9.47MYY64 pKa = 11.11EE65 pKa = 3.92PDD67 pKa = 3.2FGNFVFDD74 pKa = 5.67NDD76 pKa = 4.29ADD78 pKa = 3.78WEE80 pKa = 4.37LFDD83 pKa = 4.73GRR85 pKa = 11.84SNLFYY90 pKa = 10.26LTNSEE95 pKa = 3.76WKK97 pKa = 9.96AYY99 pKa = 10.06QSLSEE104 pKa = 4.08ASAA107 pKa = 3.44

Molecular weight: 12.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A1YZX0|A1YZX0_9CAUD Uncharacterized protein OS=Burkholderia virus BcepF1 OX=417280 GN=BcepF1.066 PE=4 SV=1
MM1 pKa = 8.09RR2 pKa = 11.84IRR4 pKa = 11.84FRR6 pKa = 11.84THH8 pKa = 6.22ADD10 pKa = 2.98RR11 pKa = 11.84VAAAEE16 pKa = 4.06DD17 pKa = 3.55QEE19 pKa = 5.99KK20 pKa = 10.33RR21 pKa = 11.84DD22 pKa = 3.16EE23 pKa = 4.05WSRR26 pKa = 11.84FFAIWPRR33 pKa = 11.84EE34 pKa = 3.84TRR36 pKa = 11.84AGEE39 pKa = 3.83WQFFCWLEE47 pKa = 3.7RR48 pKa = 11.84CPRR51 pKa = 11.84VVKK54 pKa = 10.44RR55 pKa = 11.84VVLAGMPRR63 pKa = 11.84APGSDD68 pKa = 2.84LQYY71 pKa = 10.45RR72 pKa = 11.84KK73 pKa = 10.55NITWRR78 pKa = 11.84YY79 pKa = 7.57RR80 pKa = 11.84APEE83 pKa = 3.96VKK85 pKa = 10.21KK86 pKa = 10.96

Molecular weight: 10.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

127

0

127

22644

31

956

178.3

19.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.272 ± 0.35

1.122 ± 0.085

6.002 ± 0.197

6.505 ± 0.274

4.178 ± 0.165

7.344 ± 0.284

1.771 ± 0.125

5.781 ± 0.172

5.657 ± 0.238

7.154 ± 0.2

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.773 ± 0.13

4.279 ± 0.195

4.209 ± 0.186

4.001 ± 0.191

6.302 ± 0.297

5.874 ± 0.182

5.754 ± 0.267

6.752 ± 0.206

1.479 ± 0.09

2.791 ± 0.137
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski