Thermodesulfatator indicus (strain DSM 15286 / JCM 11887 / CIR29812)

Taxonomy: cellular organisms; Bacteria; Thermodesulfobacteria; Thermodesulfobacteria; Thermodesulfobacteriales; Thermodesulfobacteriaceae; Thermodesulfatator; Thermodesulfatator indicus

Average proteome isoelectric point is 7.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2184 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F8AAB1|F8AAB1_THEID Primosomal protein N' OS=Thermodesulfatator indicus (strain DSM 15286 / JCM 11887 / CIR29812) OX=667014 GN=priA PE=3 SV=1
MM1 pKa = 7.26FKK3 pKa = 10.49KK4 pKa = 10.59VLSSLFLAVLMLGLAVSNAKK24 pKa = 9.67AAPIVMEE31 pKa = 4.02GNYY34 pKa = 10.1VYY36 pKa = 10.01TQVSEE41 pKa = 4.96DD42 pKa = 3.43GTLGNGFNFPGIQYY56 pKa = 9.88DD57 pKa = 3.78PSGTASFPGSGGKK70 pKa = 9.96DD71 pKa = 3.19FLQPGDD77 pKa = 3.97PFEE80 pKa = 4.64GFYY83 pKa = 11.0LKK85 pKa = 10.86SNEE88 pKa = 4.28SGIIGNNNDD97 pKa = 2.53WSVFIGTLTDD107 pKa = 4.07LSGSGYY113 pKa = 10.81DD114 pKa = 4.44NFIRR118 pKa = 11.84WEE120 pKa = 4.47GSLGTYY126 pKa = 9.98FDD128 pKa = 4.11VAIEE132 pKa = 4.53TYY134 pKa = 10.75FNDD137 pKa = 2.89SDD139 pKa = 4.16KK140 pKa = 11.03FIKK143 pKa = 10.67FSTTVTALNDD153 pKa = 3.41LTDD156 pKa = 3.46VYY158 pKa = 10.19FLRR161 pKa = 11.84VIDD164 pKa = 4.88PDD166 pKa = 3.15QDD168 pKa = 3.82TNDD171 pKa = 3.43FGIFDD176 pKa = 4.22TNNARR181 pKa = 11.84GFGSFSPEE189 pKa = 3.48DD190 pKa = 3.25WVYY193 pKa = 11.17AAGPISNWTIGLYY206 pKa = 10.13SDD208 pKa = 4.59SLVPHH213 pKa = 5.7NTGVSFAWSGDD224 pKa = 3.1PEE226 pKa = 4.44FYY228 pKa = 11.21YY229 pKa = 11.02NGNNDD234 pKa = 3.08GNGDD238 pKa = 3.79YY239 pKa = 10.86TIGLAFYY246 pKa = 10.67LGDD249 pKa = 3.88LSVGDD254 pKa = 4.41SVTFDD259 pKa = 3.33YY260 pKa = 11.18YY261 pKa = 11.48YY262 pKa = 11.64VLGTSPADD270 pKa = 3.2AASNIPVASPIPEE283 pKa = 4.53PSTVVLIGVGLGALGFYY300 pKa = 10.46RR301 pKa = 11.84SRR303 pKa = 11.84RR304 pKa = 11.84KK305 pKa = 9.76

Molecular weight: 32.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F8ABU2|F8ABU2_THEID Cysteine--tRNA ligase OS=Thermodesulfatator indicus (strain DSM 15286 / JCM 11887 / CIR29812) OX=667014 GN=cysS PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 9.91QPSRR9 pKa = 11.84IKK11 pKa = 10.5RR12 pKa = 11.84KK13 pKa = 8.27RR14 pKa = 11.84CHH16 pKa = 5.7GFRR19 pKa = 11.84ARR21 pKa = 11.84MRR23 pKa = 11.84TRR25 pKa = 11.84SGRR28 pKa = 11.84RR29 pKa = 11.84ILANRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.74GRR39 pKa = 11.84WRR41 pKa = 11.84LTVV44 pKa = 3.0

Molecular weight: 5.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2184

0

2184

694740

35

1408

318.1

35.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.625 ± 0.059

1.152 ± 0.025

4.362 ± 0.031

8.21 ± 0.067

5.003 ± 0.044

6.765 ± 0.044

1.811 ± 0.022

7.25 ± 0.049

7.993 ± 0.059

11.425 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.831 ± 0.018

3.277 ± 0.039

4.812 ± 0.032

2.811 ± 0.029

5.345 ± 0.042

4.953 ± 0.036

4.299 ± 0.033

6.707 ± 0.043

1.098 ± 0.02

3.272 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski