Haloarcula hispanica pleomorphic virus 2
Average proteome isoelectric point is 4.95
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|W0FES0|W0FES0_9VIRU Gp7 OS=Haloarcula hispanica pleomorphic virus 2 OX=1442594 PE=4 SV=1
MM1 pKa = 7.86 SDD3 pKa = 3.45 TEE5 pKa = 4.33 ASSSDD10 pKa = 2.97 SSPRR14 pKa = 11.84 MGRR17 pKa = 11.84 RR18 pKa = 11.84 TFLQTVGVTAGAAAGVKK35 pKa = 8.87 YY36 pKa = 9.4 TDD38 pKa = 3.65 GTALDD43 pKa = 4.21 PVGEE47 pKa = 4.28 ADD49 pKa = 4.19 AVVPLALAGVALAGAAGVGYY69 pKa = 10.75 VGGSYY74 pKa = 11.01 LEE76 pKa = 4.38 DD77 pKa = 4.13 KK78 pKa = 11.09 YY79 pKa = 11.49 LGDD82 pKa = 3.65 SRR84 pKa = 11.84 DD85 pKa = 3.5 YY86 pKa = 11.32 SGYY89 pKa = 9.14 TGSDD93 pKa = 3.11 ALKK96 pKa = 10.69 SAIQEE101 pKa = 4.35 GATSMKK107 pKa = 10.46 VADD110 pKa = 4.6 EE111 pKa = 4.25 KK112 pKa = 11.8 VMASIQNNIANSQNAGLAKK131 pKa = 10.21 GKK133 pKa = 10.2 AAIIKK138 pKa = 9.24 EE139 pKa = 4.12 MNAGNGEE146 pKa = 4.35 SAATTAMQSSINEE159 pKa = 3.77 YY160 pKa = 10.08 FAAIQQNILTHH171 pKa = 6.3 YY172 pKa = 7.31 NTQLEE177 pKa = 4.55 QVKK180 pKa = 9.65 HH181 pKa = 6.2 HH182 pKa = 6.61 VDD184 pKa = 3.32 QVANHH189 pKa = 6.35 SNLTVGTVFASEE201 pKa = 4.6 NGDD204 pKa = 3.63 DD205 pKa = 4.3 SPSNMTVNQRR215 pKa = 11.84 SLSLVDD221 pKa = 3.65 GTDD224 pKa = 3.02 ATEE227 pKa = 4.47 KK228 pKa = 10.86 YY229 pKa = 10.83 LIFNTSQDD237 pKa = 2.84 GSTYY241 pKa = 10.58 EE242 pKa = 4.1 SGISLTTATGAADD255 pKa = 3.77 FEE257 pKa = 4.75 VDD259 pKa = 3.99 SFSLSSGAYY268 pKa = 9.42 ADD270 pKa = 4.3 YY271 pKa = 11.02 SDD273 pKa = 4.39 EE274 pKa = 4.05 YY275 pKa = 11.2 QYY277 pKa = 11.34 FQTVPMGNAFDD288 pKa = 4.54 AVVTEE293 pKa = 4.11 RR294 pKa = 11.84 DD295 pKa = 3.34 KK296 pKa = 11.67 VLNDD300 pKa = 3.66 LSTFVSDD307 pKa = 4.69 VYY309 pKa = 11.0 SQYY312 pKa = 11.33 SAGDD316 pKa = 3.41 IPTEE320 pKa = 4.79 DD321 pKa = 3.11 IVDD324 pKa = 5.43 PITAATEE331 pKa = 3.59 LRR333 pKa = 11.84 TNYY336 pKa = 10.73 DD337 pKa = 3.19 GMQGASAHH345 pKa = 6.03 AAMLGIPTNAEE356 pKa = 3.38 QSVYY360 pKa = 7.89 MTLEE364 pKa = 3.91 SDD366 pKa = 5.1 GVDD369 pKa = 2.87 VWADD373 pKa = 2.99 VFTNKK378 pKa = 10.32 KK379 pKa = 7.97 ITDD382 pKa = 3.58 GSGNEE387 pKa = 3.78 VGFQKK392 pKa = 10.12 GTTYY396 pKa = 11.31 DD397 pKa = 3.43 PTTWDD402 pKa = 3.72 DD403 pKa = 3.62 PLYY406 pKa = 10.51 IAFEE410 pKa = 4.19 YY411 pKa = 11.09 SEE413 pKa = 4.32 TTDD416 pKa = 3.64 ADD418 pKa = 4.11 GNVLNSTQQDD428 pKa = 4.02 TYY430 pKa = 11.55 DD431 pKa = 3.99 GEE433 pKa = 4.77 TTTTEE438 pKa = 3.86 YY439 pKa = 11.32 SDD441 pKa = 4.05 FVAVEE446 pKa = 4.02 QPFTITEE453 pKa = 3.86 ITVDD457 pKa = 3.88 GEE459 pKa = 4.38 SQQSFKK465 pKa = 8.68 TTSRR469 pKa = 11.84 NTQTSDD475 pKa = 2.98 VSKK478 pKa = 10.57 LQEE481 pKa = 4.07 EE482 pKa = 4.77 LDD484 pKa = 4.21 QIRR487 pKa = 11.84 QTQLEE492 pKa = 4.4 LQEE495 pKa = 4.57 EE496 pKa = 4.63 SQSGGGGFDD505 pKa = 4.36 LSGLSLGGIPGEE517 pKa = 4.13 GVVLIAAGAAAYY529 pKa = 10.43 LFGKK533 pKa = 10.04
Molecular weight: 56.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.937
IPC2_protein 3.897
IPC_protein 3.91
Toseland 3.694
ProMoST 4.062
Dawson 3.897
Bjellqvist 4.05
Wikipedia 3.808
Rodwell 3.732
Grimsley 3.605
Solomon 3.884
Lehninger 3.846
Nozaki 3.999
DTASelect 4.228
Thurlkill 3.732
EMBOSS 3.821
Sillero 4.024
Patrickios 2.804
IPC_peptide 3.884
IPC2_peptide 4.012
IPC2.peptide.svr19 3.94
Protein with the highest isoelectric point:
>tr|W0FJS3|W0FJS3_9VIRU Gp8 OS=Haloarcula hispanica pleomorphic virus 2 OX=1442594 PE=4 SV=1
MM1 pKa = 7.48 LRR3 pKa = 11.84 TSAYY7 pKa = 10.12 RR8 pKa = 11.84 IYY10 pKa = 10.42 TRR12 pKa = 11.84 PTTAITLIPTLFISIRR28 pKa = 11.84 IKK30 pKa = 10.8 CLTNFRR36 pKa = 11.84 NAVIAFMLEE45 pKa = 3.66 RR46 pKa = 11.84 RR47 pKa = 11.84 KK48 pKa = 10.38 LIPVGAGRR56 pKa = 11.84 TLVTHH61 pKa = 7.11 RR62 pKa = 11.84 GFPRR66 pKa = 11.84 QRR68 pKa = 11.84 PVLPHH73 pKa = 7.5 RR74 pKa = 11.84 ILQWQLTLTKK84 pKa = 9.82 LLPHH88 pKa = 6.45 ATINVPDD95 pKa = 3.74 NRR97 pKa = 11.84 ITANLALAQEE107 pKa = 4.67 TPYY110 pKa = 10.93 SRR112 pKa = 11.84 SQARR116 pKa = 11.84 SEE118 pKa = 4.05 TGIPLAHH125 pKa = 5.84 RR126 pKa = 11.84 TVVKK130 pKa = 9.41 PHH132 pKa = 4.98 QEE134 pKa = 3.74 RR135 pKa = 11.84 YY136 pKa = 7.04 STHH139 pKa = 5.49 IHH141 pKa = 5.37 TQAQDD146 pKa = 3.27 TSRR149 pKa = 11.84 GPTGGLLSGVLWVGGFVGGNPHH171 pKa = 4.5 QQEE174 pKa = 4.38 RR175 pKa = 11.84 TEE177 pKa = 4.4 TSCPAA182 pKa = 3.7
Molecular weight: 20.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.179
IPC2_protein 10.116
IPC_protein 11.389
Toseland 11.477
ProMoST 11.93
Dawson 11.491
Bjellqvist 11.462
Wikipedia 11.945
Rodwell 11.242
Grimsley 11.535
Solomon 11.959
Lehninger 11.857
Nozaki 11.477
DTASelect 11.462
Thurlkill 11.477
EMBOSS 11.974
Sillero 11.491
Patrickios 10.979
IPC_peptide 11.959
IPC2_peptide 10.935
IPC2.peptide.svr19 9.298
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
0
8
2525
115
725
315.6
34.23
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.139 ± 0.696
1.267 ± 0.529
8.436 ± 0.904
8.396 ± 0.799
3.168 ± 0.642
7.921 ± 0.56
1.782 ± 0.49
3.406 ± 0.626
2.614 ± 0.257
7.802 ± 1.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.178 ± 0.206
3.05 ± 0.594
4.554 ± 0.681
3.01 ± 0.636
5.822 ± 1.172
7.921 ± 0.743
7.089 ± 0.977
7.881 ± 0.894
1.109 ± 0.236
2.455 ± 0.604
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here