Tobacco leaf chlorosis betasatellite
Average proteome isoelectric point is 5.93
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|I7CPF9|I7CPF9_9VIRU C1 OS=Tobacco leaf chlorosis betasatellite OX=1213187 GN=C1 PE=4 SV=1
MM1 pKa = 7.35 FRR3 pKa = 11.84 PTEE6 pKa = 3.66 LTMTIKK12 pKa = 10.69 YY13 pKa = 9.86 DD14 pKa = 3.55 NKK16 pKa = 9.82 RR17 pKa = 11.84 GMEE20 pKa = 3.79 FTVNVRR26 pKa = 11.84 LTNNYY31 pKa = 9.97 SIIVQVDD38 pKa = 4.04 LVSTRR43 pKa = 11.84 SPAIAKK49 pKa = 7.86 TKK51 pKa = 10.08 FLIPYY56 pKa = 7.56 GHH58 pKa = 7.1 HH59 pKa = 6.83 GFTGPFDD66 pKa = 4.31 FNRR69 pKa = 11.84 LEE71 pKa = 3.99 EE72 pKa = 4.34 RR73 pKa = 11.84 VCQLLKK79 pKa = 11.14 VMYY82 pKa = 9.34 KK83 pKa = 10.38 DD84 pKa = 2.94 KK85 pKa = 10.24 HH86 pKa = 5.53 TGEE89 pKa = 4.36 FRR91 pKa = 11.84 QEE93 pKa = 4.03 DD94 pKa = 4.14 MVEE97 pKa = 4.27 TIDD100 pKa = 3.74 ILMMHH105 pKa = 7.28 EE106 pKa = 4.77 APVIDD111 pKa = 4.29 INVHH115 pKa = 5.79 DD116 pKa = 5.56 LYY118 pKa = 11.42 SVYY121 pKa = 10.54 TSASVV126 pKa = 2.8
Molecular weight: 14.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.928
IPC2_protein 5.855
IPC_protein 5.919
Toseland 6.427
ProMoST 6.287
Dawson 6.224
Bjellqvist 6.186
Wikipedia 6.237
Rodwell 6.211
Grimsley 6.62
Solomon 6.224
Lehninger 6.211
Nozaki 6.478
DTASelect 6.678
Thurlkill 6.707
EMBOSS 6.678
Sillero 6.59
Patrickios 4.151
IPC_peptide 6.237
IPC2_peptide 6.561
IPC2.peptide.svr19 6.537
Protein with the highest isoelectric point:
>tr|I7CPF9|I7CPF9_9VIRU C1 OS=Tobacco leaf chlorosis betasatellite OX=1213187 GN=C1 PE=4 SV=1
MM1 pKa = 7.35 FRR3 pKa = 11.84 PTEE6 pKa = 3.66 LTMTIKK12 pKa = 10.69 YY13 pKa = 9.86 DD14 pKa = 3.55 NKK16 pKa = 9.82 RR17 pKa = 11.84 GMEE20 pKa = 3.79 FTVNVRR26 pKa = 11.84 LTNNYY31 pKa = 9.97 SIIVQVDD38 pKa = 4.04 LVSTRR43 pKa = 11.84 SPAIAKK49 pKa = 7.86 TKK51 pKa = 10.08 FLIPYY56 pKa = 7.56 GHH58 pKa = 7.1 HH59 pKa = 6.83 GFTGPFDD66 pKa = 4.31 FNRR69 pKa = 11.84 LEE71 pKa = 3.99 EE72 pKa = 4.34 RR73 pKa = 11.84 VCQLLKK79 pKa = 11.14 VMYY82 pKa = 9.34 KK83 pKa = 10.38 DD84 pKa = 2.94 KK85 pKa = 10.24 HH86 pKa = 5.53 TGEE89 pKa = 4.36 FRR91 pKa = 11.84 QEE93 pKa = 4.03 DD94 pKa = 4.14 MVEE97 pKa = 4.27 TIDD100 pKa = 3.74 ILMMHH105 pKa = 7.28 EE106 pKa = 4.77 APVIDD111 pKa = 4.29 INVHH115 pKa = 5.79 DD116 pKa = 5.56 LYY118 pKa = 11.42 SVYY121 pKa = 10.54 TSASVV126 pKa = 2.8
Molecular weight: 14.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.928
IPC2_protein 5.855
IPC_protein 5.919
Toseland 6.427
ProMoST 6.287
Dawson 6.224
Bjellqvist 6.186
Wikipedia 6.237
Rodwell 6.211
Grimsley 6.62
Solomon 6.224
Lehninger 6.211
Nozaki 6.478
DTASelect 6.678
Thurlkill 6.707
EMBOSS 6.678
Sillero 6.59
Patrickios 4.151
IPC_peptide 6.237
IPC2_peptide 6.561
IPC2.peptide.svr19 6.537
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
126
126
126
126.0
14.66
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.175 ± 0.0
0.794 ± 0.0
6.349 ± 0.0
6.349 ± 0.0
5.556 ± 0.0
3.968 ± 0.0
3.968 ± 0.0
7.143 ± 0.0
5.556 ± 0.0
7.143 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
5.556 ± 0.0
4.762 ± 0.0
3.968 ± 0.0
2.381 ± 0.0
5.556 ± 0.0
4.762 ± 0.0
8.73 ± 0.0
9.524 ± 0.0
0.0 ± 0.0
4.762 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here